##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1727991 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.349195684468263 31.0 31.0 34.0 26.0 34.0 2 30.390466154048255 31.0 31.0 34.0 26.0 34.0 3 30.472689383220168 31.0 31.0 34.0 26.0 34.0 4 33.838791984448996 37.0 35.0 37.0 28.0 37.0 5 33.92292205225606 37.0 35.0 37.0 30.0 37.0 6 34.02875940904785 37.0 35.0 37.0 30.0 37.0 7 33.938082432142295 37.0 35.0 37.0 29.0 37.0 8 33.92609857343007 37.0 35.0 37.0 29.0 37.0 9 35.37435322290452 39.0 35.0 39.0 29.0 39.0 10-11 35.31552971051354 39.0 35.0 39.0 28.5 39.0 12-13 35.26088359256501 39.0 35.0 39.0 28.0 39.0 14-15 36.48816486891425 40.0 37.0 41.0 28.0 41.0 16-17 36.50864587836395 40.0 37.0 41.0 28.5 41.0 18-19 36.39047743882925 40.0 37.0 41.0 27.5 41.0 20-21 36.33573323009206 40.0 36.5 41.0 27.0 41.0 22-23 36.3611034432471 40.0 36.5 41.0 27.0 41.0 24-25 36.22209693221782 40.0 36.0 41.0 27.0 41.0 26-27 36.19939021673145 40.0 36.0 41.0 27.0 41.0 28-29 36.27469356032526 40.0 36.0 41.0 28.0 41.0 30-31 36.157690925473574 40.0 36.0 41.0 27.5 41.0 32-33 35.96436005743085 40.0 36.0 41.0 27.0 41.0 34-35 35.917586665671294 40.0 35.5 41.0 27.0 41.0 36-37 35.94472801073617 40.0 35.5 41.0 27.0 41.0 38-39 35.785544889990746 40.0 35.0 41.0 26.5 41.0 40-41 35.7778810769269 40.0 35.0 41.0 27.0 41.0 42-43 35.69893737872478 40.0 35.0 41.0 27.0 41.0 44-45 35.67426074557101 40.0 35.0 41.0 27.0 41.0 46-47 35.59174411209318 40.0 35.0 41.0 27.0 41.0 48-49 35.48361797023249 39.0 35.0 41.0 27.0 41.0 50-51 35.349882898695654 39.0 35.0 41.0 26.5 41.0 52-53 35.188732753816424 39.0 35.0 41.0 26.0 41.0 54-55 34.993682258761766 39.0 34.0 41.0 26.0 41.0 56-57 34.786891251169706 38.5 34.0 40.5 26.0 41.0 58-59 34.542272789615225 38.0 34.0 40.0 26.0 41.0 60-61 34.27944908277878 38.0 34.0 40.0 25.0 41.0 62-63 33.9725377620601 37.0 33.0 40.0 24.0 41.0 64-65 33.65304217440948 37.0 33.0 40.0 23.0 41.0 66-67 33.34124801575935 36.0 33.0 39.0 23.0 41.0 68-69 33.1047679067773 36.0 33.0 39.0 22.5 41.0 70-71 32.934604115414956 35.0 33.0 39.0 23.0 41.0 72-73 32.627067502087684 35.0 33.0 38.0 22.5 40.0 74-75 32.30236615815707 35.0 33.0 37.0 22.0 39.5 76-77 31.982554596638522 35.0 32.0 37.0 21.5 39.0 78-79 31.656978826857316 35.0 32.0 36.0 20.0 39.0 80-81 31.379952499752605 35.0 32.0 36.0 20.0 37.5 82-83 31.130628863229035 35.0 32.0 35.5 19.5 37.0 84-85 30.905670515645046 35.0 32.0 35.0 18.0 37.0 86-87 30.708735172810506 35.0 32.0 35.0 18.0 36.0 88-89 30.54817154718977 35.0 32.0 35.0 16.5 36.0 90-91 30.40991677618691 35.0 31.0 35.0 14.0 36.0 92-93 30.289164700510593 35.0 31.0 35.0 10.5 35.5 94-95 30.166811922052837 35.0 31.0 35.0 8.5 35.0 96-97 30.070726641516075 35.0 31.0 35.0 7.0 35.0 98-99 29.995350091522468 35.0 31.0 35.0 7.0 35.0 100-101 29.89495170981793 35.0 31.0 35.0 3.5 35.0 102-103 28.90810658157363 33.5 29.5 34.5 2.0 35.0 104-105 29.60094178731255 34.0 30.5 35.0 2.0 35.0 106-107 29.716917796446857 34.5 31.0 35.0 2.0 35.0 108-109 29.687382631043796 35.0 31.0 35.0 2.0 35.0 110-111 29.612209496461496 34.0 31.0 35.0 2.0 35.0 112-113 29.773514734741095 35.0 31.0 35.0 2.0 35.0 114-115 29.763155595139096 35.0 31.0 35.0 2.0 35.0 116-117 29.67056599253121 35.0 31.0 35.0 2.0 35.0 118-119 29.42738040881 35.0 31.0 35.0 2.0 35.0 120-121 29.35415288621295 35.0 31.0 35.0 2.0 35.0 122-123 29.200200984843093 35.0 31.0 35.0 2.0 35.0 124-125 28.288803298165327 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7690860552881134 1101 2 0.8427401033190591 1101 3 0.7946067922971487 1101 4 0.8001036016998668 1101 5 0.5922632783521351 1101 6 0.8758331035145019 1101 7 0.9021313280753205 1101 8 0.805874178148251 1101 9 0.9613823880223862 1101 10-11 0.9801193905326357 1101 12-13 0.975189251729006 1101 14-15 1.07343827262779 1101 16-17 1.0651519698704064 1101 18-19 1.1410922240390278 1101 20-21 1.2214194882913887 1101 22-23 1.153728075597023 1101 24-25 1.4252833679634094 1101 26-27 1.3944084360038502 1101 28-29 1.2899743821231198 1101 30-31 1.3812989217893303 1101 32-33 1.5468699278626161 1101 34-35 1.4417547548313223 1101 36-37 1.4267181728408218 1101 38-39 1.417195743974652 1101 40-41 1.337237565677306 1101 42-43 1.253290858319744 1101 44-45 1.1527247992132175 1101 46-47 1.0845314014057266 1101 48-49 1.1483005425642148 1101 50-51 1.0904968629133407 1101 52-53 1.095165148274667 1101 54-55 1.061708798397646 1101 56-57 1.1096110802854255 1101 58-59 1.183504361519951 1101 60-61 0.9195713123758935 1101 62-63 0.8350562203532945 1101 64-65 0.8820302900973118 1101 66-67 0.7403979178611806 1101 68-69 0.8821016013134368 1101 70-71 0.8258382293506585 1101 72-73 0.8099806179584448 1101 74-75 0.8016615431233021 1101 76-77 0.7739572864913526 1101 78-79 0.7991705093582766 1101 80-81 0.8122066248508197 1101 82-83 0.760770712975944 1101 84-85 0.7912232944303597 1101 86-87 0.8037025929537052 1101 88-89 0.7852644960364366 1101 90-91 0.7806372368864345 1101 92-93 0.8260090288667641 1101 94-95 0.8326228817676196 1101 96-97 0.7718173119015326 1101 98-99 0.6913459761832677 1101 100-101 0.6179265355861645 1101 102-103 0.4540057578810277 1101 104-105 0.6208955098425406 1101 106-107 0.5721548646060199 1101 108-109 0.5315127182642065 1101 110-111 0.5182136683469807 1101 112-113 0.5995968697376703 1101 114-115 0.7589145819874012 1101 116-117 0.7681225600471429 1101 118-119 0.5766904732393137 1101 120-121 0.6651415271339225 1101 122-123 0.7219477067863487 1101 124-125 0.5328973311512826 1102 1 0.06922407604466585 1102 2 0.06791233749857639 1102 3 0.057940506462117014 1102 4 0.08205583681303352 1102 5 0.10212773914367546 1102 6 0.2386200785810857 1102 7 0.2374511283721219 1102 8 0.326281993550559 1102 9 0.32347803081704996 1102 10-11 0.4562530710370041 1102 12-13 0.4113641726143058 1102 14-15 0.5180574440609931 1102 16-17 0.4166161907380115 1102 18-19 0.5813589351770005 1102 20-21 0.5459621371351346 1102 22-23 0.564340440586065 1102 24-25 0.8340969944879717 1102 26-27 0.8710990827788265 1102 28-29 0.6945983231654651 1102 30-31 0.7498133436377685 1102 32-33 0.9110515452423016 1102 34-35 0.8271812494112254 1102 36-37 0.7374577478818551 1102 38-39 0.8449495252642691 1102 40-41 0.766735494444589 1102 42-43 0.7967729248350537 1102 44-45 0.6296123113752614 1102 46-47 0.5842867785027579 1102 48-49 0.5998059331746362 1102 50-51 0.5356710043222748 1102 52-53 0.6351692084313214 1102 54-55 0.553855758255537 1102 56-57 0.5753355418386334 1102 58-59 0.6069141283772836 1102 60-61 0.6044834231905227 1102 62-63 0.6100169701248035 1102 64-65 0.5954697618224074 1102 66-67 0.5666873247220465 1102 68-69 0.539954242196572 1102 70-71 0.5155608733541612 1102 72-73 0.47880213138337524 1102 74-75 0.35373934825543785 1102 76-77 0.3191801221391657 1102 78-79 0.4403809612211518 1102 80-81 0.41784816952851145 1102 82-83 0.4045579976217972 1102 84-85 0.37480915906559176 1102 86-87 0.34730512818677184 1102 88-89 0.3371558779393169 1102 90-91 0.3584288556420212 1102 92-93 0.4120898014144565 1102 94-95 0.37290673032930144 1102 96-97 0.3154718275819697 1102 98-99 0.38459423050017705 1102 100-101 0.39254952762063766 1102 102-103 0.32467080355625555 1102 104-105 0.45226625497035045 1102 106-107 0.45866995413830836 1102 108-109 0.452102181144177 1102 110-111 0.45559174079835785 1102 112-113 0.4487985207042442 1102 114-115 0.4050578017100612 1102 116-117 0.48152932324848763 1102 118-119 0.2952665652433204 1102 120-121 0.2970020248194487 1102 122-123 0.3183537003392374 1102 124-125 0.336416154062114 1103 1 0.4338022050993331 1103 2 0.10683113904741148 1103 3 0.42745299177644824 1103 4 0.48712010048458154 1103 5 0.3659742019670489 1103 6 0.38427463544329044 1103 7 0.5580826110873005 1103 8 0.5670346351041857 1103 9 0.7351977875285911 1103 10-11 0.7124186827384946 1103 12-13 0.6975840493099668 1103 14-15 0.8122933519574147 1103 16-17 0.6593483190984841 1103 18-19 0.9213783738982855 1103 20-21 0.9387810547332549 1103 22-23 0.9138691420677105 1103 24-25 1.1291378228394322 1103 26-27 1.1063407836611958 1103 28-29 0.9984921994656375 1103 30-31 1.0650791447432866 1103 32-33 1.1622206186043798 1103 34-35 1.2328068750914838 1103 36-37 1.1263205640177745 1103 38-39 1.1503318940740854 1103 40-41 1.2271316847654106 1103 42-43 1.1229959120707917 1103 44-45 0.9595299624838134 1103 46-47 0.9007947851276654 1103 48-49 0.9328412329216889 1103 50-51 0.9814024917621396 1103 52-53 0.9188640331446365 1103 54-55 0.9049100748048318 1103 56-57 0.9613005725326005 1103 58-59 0.8715647936178286 1103 60-61 0.727148561489642 1103 62-63 0.764876341689444 1103 64-65 0.7467252295013012 1103 66-67 0.7771655508698032 1103 68-69 0.8542942770731017 1103 70-71 0.9016285834638893 1103 72-73 0.8593818780337656 1103 74-75 0.9612343568151687 1103 76-77 0.9602759283528002 1103 78-79 0.9270210049582772 1103 80-81 0.903228918444885 1103 82-83 0.8699972551361732 1103 84-85 0.8435300650685491 1103 86-87 0.8953358303373378 1103 88-89 0.8130334674172452 1103 90-91 0.8977066761270969 1103 92-93 0.8696207334089436 1103 94-95 0.892692766531912 1103 96-97 0.8416199665498674 1103 98-99 0.781006855846254 1103 100-101 0.8572028208797988 1103 102-103 0.8069362015553132 1103 104-105 0.7821686836760122 1103 106-107 0.7845037648471518 1103 108-109 0.7577599892818725 1103 110-111 0.7691914786283718 1103 112-113 0.8315972197919521 1103 114-115 0.88248764546071 1103 116-117 0.9506065914475528 1103 118-119 0.8549901551282382 1103 120-121 0.7902261886320332 1103 122-123 0.9521672382540061 1103 124-125 0.9151154744105483 1104 1 0.7546315261723677 1104 2 0.8088699858975374 1104 3 0.7861467515590697 1104 4 0.8826271159581935 1104 5 0.9283059934252265 1104 6 0.8266676350266025 1104 7 0.8440088742052012 1104 8 0.9289084161065517 1104 9 1.064624959314024 1104 10-11 1.0691415268853817 1104 12-13 1.0520232141061925 1104 14-15 1.114019014899533 1104 16-17 1.1230600246271791 1104 18-19 1.2954198959776804 1104 20-21 1.38937412224778 1104 22-23 1.3709689694383869 1104 24-25 1.5455214311074386 1104 26-27 1.4736583760949173 1104 28-29 1.3674215124849667 1104 30-31 1.3408185269894304 1104 32-33 1.410119688226871 1104 34-35 1.4178639688260546 1104 36-37 1.3438922682158534 1104 38-39 1.3638923651105301 1104 40-41 1.2919419150662321 1104 42-43 1.238663791461363 1104 44-45 1.1554647345115683 1104 46-47 1.1889917716429679 1104 48-49 1.2073791430914156 1104 50-51 1.0185581347801005 1104 52-53 1.1211690723101313 1104 54-55 1.1241586995478983 1104 56-57 1.0663092740309352 1104 58-59 1.1623862360706312 1104 60-61 1.1644956024549629 1104 62-63 1.0616998220786797 1104 64-65 1.1319635665171575 1104 66-67 1.1034323804780044 1104 68-69 1.0923171986055493 1104 70-71 1.166834215451786 1104 72-73 1.044986833510805 1104 74-75 1.093513028864976 1104 76-77 1.0056074781999556 1104 78-79 1.0570824581960423 1104 80-81 1.1061021286849915 1104 82-83 1.1032245830933682 1104 84-85 1.1550774610970223 1104 86-87 1.217702637885406 1104 88-89 1.1488302760508127 1104 90-91 1.2180982960764624 1104 92-93 1.2060393577668371 1104 94-95 1.2190524588105127 1104 96-97 1.2131609196178026 1104 98-99 1.2223990255482349 1104 100-101 1.3494936785288871 1104 102-103 1.2389074323358535 1104 104-105 1.2743262700869664 1104 106-107 1.3158346213757284 1104 108-109 1.285104334008274 1104 110-111 1.2594786455815878 1104 112-113 1.327568128424467 1104 114-115 1.3226975319035326 1104 116-117 1.19906724911975 1104 118-119 1.1204600185304692 1104 120-121 1.2535653229642563 1104 122-123 1.2424146969612835 1104 124-125 1.2385482417671447 1105 1 0.4989700484136286 1105 2 0.5275016694365569 1105 3 0.5217696365150744 1105 4 0.5008185823406919 1105 5 0.5664531068851844 1105 6 0.35870369337661856 1105 7 0.4233720929601503 1105 8 0.5120845461700583 1105 9 0.5759212742773769 1105 10-11 0.6256471502023473 1105 12-13 0.6032011586074333 1105 14-15 0.601746431629806 1105 16-17 0.6377892989960827 1105 18-19 0.7499955911521425 1105 20-21 0.8824404500274028 1105 22-23 0.7695658042525508 1105 24-25 0.9962212136629134 1105 26-27 0.998068899833811 1105 28-29 0.8970430726134211 1105 30-31 0.920240426964952 1105 32-33 1.056321105216341 1105 34-35 1.0728798091555234 1105 36-37 0.9533740734374021 1105 38-39 1.1403793400396012 1105 40-41 1.023400449403752 1105 42-43 0.9717667680032775 1105 44-45 0.9559242480694508 1105 46-47 0.754565551577592 1105 48-49 0.7634530454285908 1105 50-51 0.7064237943858203 1105 52-53 0.7777420170273857 1105 54-55 0.784547003624624 1105 56-57 0.8253173813995645 1105 58-59 0.8321029821789523 1105 60-61 0.7774860406560862 1105 62-63 0.7986651749580176 1105 64-65 0.8292614223125483 1105 66-67 0.917750094286454 1105 68-69 0.997756911751587 1105 70-71 0.9240650378528841 1105 72-73 0.8979342868178364 1105 74-75 0.8578562312035842 1105 76-77 0.8076617529288725 1105 78-79 0.757385008489095 1105 80-81 0.758418560866783 1105 82-83 0.7060612980970369 1105 84-85 0.7782746875993354 1105 86-87 0.7104887358362433 1105 88-89 0.7688487084342093 1105 90-91 0.705585875818123 1105 92-93 0.715592300468046 1105 94-95 0.7108496161913855 1105 96-97 0.7501228164252574 1105 98-99 0.7373270960520983 1105 100-101 0.8135717713532173 1105 102-103 0.7851602583946367 1105 104-105 0.8138152754582784 1105 106-107 0.9209872645507104 1105 108-109 0.8786082868904934 1105 110-111 0.8559386199490504 1105 112-113 0.8916755978598303 1105 114-115 0.8506768098725139 1105 116-117 0.8778244312691399 1105 118-119 0.768599067822695 1105 120-121 0.9003925049696448 1105 122-123 0.9258721368760341 1105 124-125 0.8914358618296419 1106 1 0.284090382577908 1106 2 0.27535173272702806 1106 3 0.25584271026933436 1106 4 0.3787346745743605 1106 5 0.3189894269175255 1106 6 0.3805967894852813 1106 7 0.2883089376510952 1106 8 0.2723752709722831 1106 9 0.41449913683421613 1106 10-11 0.4115364312531682 1106 12-13 0.4576994832023331 1106 14-15 0.48020213829111213 1106 16-17 0.5605271789005002 1106 18-19 0.5947911400162553 1106 20-21 0.5753299097254114 1106 22-23 0.6155360759490804 1106 24-25 0.778462184046262 1106 26-27 0.6963799198975309 1106 28-29 0.5763502035747408 1106 30-31 0.6630747058355695 1106 32-33 0.7893430106532122 1106 34-35 0.8043614701983302 1106 36-37 0.6243689678886426 1106 38-39 0.6861258032365214 1106 40-41 0.5858421980776995 1106 42-43 0.5460925217592774 1106 44-45 0.4743469461357108 1106 46-47 0.3874737751075443 1106 48-49 0.39601954743424983 1106 50-51 0.3856808277376089 1106 52-53 0.3628126061752681 1106 54-55 0.3794185069290137 1106 56-57 0.43576542151056685 1106 58-59 0.43716192987646707 1106 60-61 0.4825860948378988 1106 62-63 0.4428809137758236 1106 64-65 0.34416421238211825 1106 66-67 0.3707075574492862 1106 68-69 0.45110766318634177 1106 70-71 0.47285423298851725 1106 72-73 0.3656949324863987 1106 74-75 0.4879165305081514 1106 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72-73 -3.1528074047683887 2216 74-75 -3.2815161022495793 2216 76-77 -3.1414236019999997 2216 78-79 -3.073244258997274 2216 80-81 -3.143692656459894 2216 82-83 -3.2488855472429563 2216 84-85 -3.2433771525563913 2216 86-87 -3.220255169323405 2216 88-89 -3.0721421633207093 2216 90-91 -3.149751610325371 2216 92-93 -3.1916920610691797 2216 94-95 -3.101401790265772 2216 96-97 -3.2377821432507154 2216 98-99 -3.251855869951342 2216 100-101 -3.2272641791598318 2216 102-103 -3.3120666195883395 2216 104-105 -3.276443758868872 2216 106-107 -3.298731426678337 2216 108-109 -3.2831526367764816 2216 110-111 -3.284544112444369 2216 112-113 -3.344926069891468 2216 114-115 -3.4142230533829796 2216 116-117 -3.385127140599174 2216 118-119 -3.2421838667399143 2216 120-121 -3.01208667414717 2216 122-123 -2.9508545081904742 2216 124-125 -2.861644212888578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44710.0 3 7866.0 4 5548.0 5 4652.0 6 4833.0 7 5365.0 8 5538.0 9 5927.0 10 6725.0 11 7325.0 12 7680.0 13 7720.0 14 7345.0 15 7257.0 16 6673.0 17 6134.0 18 5752.0 19 5465.0 20 5477.0 21 5844.0 22 6794.0 23 8778.0 24 13067.0 25 18492.0 26 20596.0 27 21242.0 28 24509.0 29 28991.0 30 34144.0 31 41290.0 32 52855.0 33 69427.0 34 97848.0 35 152452.0 36 299445.0 37 501141.0 38 172659.0 39 425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39263238111121 17.994068010571496 12.079276950480281 24.534022657837014 2 16.70223643726384 20.280153920800036 38.23111511520321 24.78649452673291 3 19.79147729443874 24.326162952944596 28.148480950070574 27.73387880254609 4 13.190487383787378 16.31012110776727 35.58118180523407 34.91820970321128 5 14.387892753136525 37.98799233454722 33.20432137005495 14.419793542261308 6 33.34465725835622 35.29441722779898 17.140583539025585 14.220341974819217 7 29.318177346344243 32.00870840388753 20.286217596670344 18.386896653097885 8 26.491515291456956 34.893526644525345 19.597960869009157 19.01699719500854 9 26.603868254123498 16.62199942938593 18.819558057666743 37.95457425882383 10-11 25.283547365923386 26.623127094268867 27.05106688349644 21.042258656311308 12-13 25.69524079043048 24.140387610828938 26.88662743508255 23.277744163658035 14-15 22.918847841801355 25.851244289878828 24.327040552639605 26.90286731568021 16-17 23.480000567150473 28.394185781711307 25.088364068934244 23.037449582203976 18-19 23.34321717432879 27.423361649906 26.44862606700775 22.784795108757457 20-21 24.108117962652685 26.863589405238347 26.18233222500991 22.84596040709906 22-23 23.844905190673522 26.566348585652232 26.061227484213877 23.527518739460366 24-25 23.553783099005628 26.6710823752461 26.36779900944603 23.40733551630224 26-27 23.378048967540867 27.0460274973223 26.132990448837774 23.44293308629906 28-29 23.410579670177828 26.965128463041644 26.220975472409535 23.40331639437099 30-31 23.672610481837655 26.683650924879093 26.109043220442224 23.534695372841025 32-33 23.39189899687288 26.78650641093344 26.273845862704658 23.547748729489022 34-35 23.76325850208229 26.848173691080156 26.05450130667693 23.33406650016062 36-37 23.84210738034003 26.781735566297403 25.9203105135472 23.45584653981537 38-39 23.469131993426465 26.71511919019781 26.19689464687505 23.61885416950067 40-41 23.661466777823467 26.664262963574924 26.151151448052666 23.523118810548937 42-43 23.596867875468504 26.700403856676452 25.991882356379424 23.710845911475612 44-45 23.39595147417197 26.80584402544695 26.109773973994017 23.688430526387062 46-47 23.561861054605327 26.868187355500318 25.88595123108664 23.684000358807715 48-49 23.58985458641782 26.692173049483674 26.011880665546443 23.706091698552058 50-51 23.453728987812475 26.770857235617466 26.066498818980005 23.708914957590054 52-53 23.6473439946434 26.695820479796566 25.971325171307345 23.685510354252685 54-55 23.417787451797896 26.580129071388598 26.070603241774172 23.931480235039334 56-57 23.36941188725898 26.80366968229556 25.991171581230276 23.835746849215177 58-59 23.53025606359804 26.616457891069373 26.043173836648048 23.81011220868454 60-61 23.49906741119425 26.589647902507206 26.192158150855104 23.71912653544344 62-63 23.347710130432972 26.694685215281538 26.14206984206359 23.815534812221898 64-65 23.179334545369315 26.53693271174773 26.15841983533634 24.12531290754662 66-67 23.323804555004894 26.76695467131502 25.882714136360786 24.026526637319304 68-69 23.254716994782157 26.46694117418958 26.248747338773132 24.029594492255132 70-71 23.185348641655512 26.324648470527777 26.292471594937115 24.197531292879592 72-73 23.258173269877997 26.584746123189273 26.083974826592094 24.073105780340633 74-75 23.085625991041564 26.719522216692322 26.064306299841434 24.13054549242468 76-77 23.258329984065295 26.40713383964428 26.152676274942642 24.181859901347778 78-79 23.224146257689444 26.449959243293954 26.296163082381792 24.02973141663481 80-81 23.151055155111997 26.577234807820847 26.227136859101417 24.044573177965734 82-83 23.232203105377987 26.586890976116486 26.205576485702874 23.975329432802653 84-85 23.202383625768903 26.375371283976968 26.11393874711481 24.30830634313932 86-87 23.078158462891377 26.28492438914783 26.31600269345304 24.320914454507758 88-89 23.21231601157896 26.16212046329722 26.40749622966246 24.21806729546136 90-91 23.216383541920095 26.27683917272095 26.444867932346362 24.061909353012595 92-93 23.129840461009376 26.08091391665545 26.476064810996863 24.31318081133831 94-95 23.06490600934843 26.058324377846876 26.43836107942692 24.43840853337778 96-97 23.001438400103243 26.008507712700773 26.553080031007635 24.436973856188345 98-99 22.90369803527043 25.996951780713445 26.43187004143402 24.667480142582107 100-101 22.73703852903453 26.067582084299918 26.646287395759536 24.549091990906017 102-103 22.805666626733128 25.83989198267695 26.689926504930057 24.664514885659866 104-105 22.715339189501265 26.019895227958195 26.592257796407004 24.672507786133536 106-107 22.628537323524576 26.094367606093655 26.58049270711042 24.696602363271346 108-109 22.7057736234063 26.107637882173336 26.395575409191895 24.791013085228467 110-111 22.473883928958962 26.329328415778384 26.601904327591107 24.59488332767155 112-113 22.509943152631266 26.266361018847928 26.711549163872245 24.512146664648565 114-115 22.378713166465406 26.343321944572125 26.591146174036172 24.6868187149263 116-117 22.242290880256892 26.355423299914627 26.74792494933058 24.654360870497904 118-119 22.27932210752879 26.26373061553754 26.56965397121039 24.88729330572328 120-121 22.342278923979862 26.29192521393519 26.68175945399452 24.684036408090428 122-123 22.275135674529306 26.323145009677805 26.67795937172969 24.723759944063197 124-125 22.273085237285642 26.401030135707494 26.50053715794824 24.825347469058627 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1095.0 1 841.0 2 530.0 3 414.5 4 322.5 5 286.0 6 273.5 7 253.0 8 266.0 9 279.0 10 257.5 11 237.5 12 242.5 13 274.0 14 312.5 15 372.5 16 413.0 17 490.0 18 609.0 19 741.0 20 946.5 21 1224.0 22 1620.5 23 2190.0 24 2942.0 25 3992.0 26 5669.0 27 7821.0 28 9714.5 29 11954.0 30 15078.0 31 18279.0 32 21998.5 33 25563.5 34 28734.5 35 31856.0 36 35574.0 37 39389.5 38 40794.0 39 41986.0 40 44285.0 41 47015.5 42 49587.5 43 52788.0 44 56092.5 45 58406.0 46 59828.0 47 61215.0 48 63828.0 49 65932.5 50 66840.0 51 67261.5 52 68100.5 53 69445.5 54 69339.0 55 65838.0 56 60654.5 57 56026.0 58 52780.5 59 47887.0 60 40746.0 61 33734.0 62 27546.5 63 21713.0 64 16075.5 65 11841.0 66 8885.0 67 7304.5 68 5720.5 69 3942.5 70 2941.0 71 2283.0 72 1874.0 73 1570.5 74 1126.0 75 809.5 76 562.0 77 303.0 78 176.5 79 108.5 80 60.0 81 46.0 82 31.0 83 24.5 84 23.0 85 19.0 86 16.5 87 10.0 88 9.5 89 10.0 90 6.0 91 4.5 92 5.0 93 6.5 94 5.0 95 2.5 96 2.0 97 1.0 98 0.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06267393753786912 2 0.050289613776923606 3 0.04589144272163455 4 0.04450254659891169 5 0.04403958122467073 6 0.0049768777730902535 7 2.893533589005961E-4 8 0.0 9 0.0016203788098433382 10-11 0.0035301109785872728 12-13 0.002690986237775544 14-15 0.007986152705656453 16-17 0.0031539516120164977 18-19 0.005005813108980313 20-21 0.0090278247976986 22-23 0.016579947465004158 24-25 0.003993076352828227 26-27 0.01710078351102523 28-29 0.006973415949504367 30-31 0.00674193326238389 32-33 0.0025752448942153055 34-35 0.01886583900031887 36-37 0.01576975806008249 38-39 0.026504767675294603 40-41 0.005873873185682102 42-43 0.0015335728021731595 44-45 2.314826871204769E-4 46-47 0.0026620509018854843 48-49 0.0033275636273568557 50-51 0.0020544088481942325 52-53 0.001707184817513517 54-55 0.0063657738958131154 56-57 0.002806727581335782 58-59 0.008044023377436572 60-61 0.01582762873186261 62-63 0.01197922905848468 64-65 0.0025752448942153055 66-67 0.003906270345158047 68-69 0.009809078866730209 70-71 0.0024305682147650076 72-73 0.003269692955576736 74-75 6.365773895813115E-4 76-77 8.680600767017885E-5 78-79 0.0031539516120164977 80-81 0.0026620509018854843 82-83 0.0012152841073825038 84-85 0.011747746371364203 86-87 0.005642390498561624 88-89 0.0016782494816234576 90-91 7.523187331415499E-4 92-93 0.0025173742224351863 94-95 0.0 96-97 0.001707184817513517 98-99 0.02459503550655067 100-101 0.014757021303930402 102-103 0.006944480613614308 104-105 0.013888961227228616 106-107 0.03211822283796617 108-109 6.944480613614308E-4 110-111 0.0018518614969638152 112-113 0.01704291283924511 114-115 0.03223396418152641 116-117 0.05002919575391307 118-119 0.3561650494707438 120-121 0.11579921423201857 122-123 0.018923709672098988 124-125 0.3182597594547657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1727991.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.14956561679584 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6560998431029 69.28435692017774 2 7.466488578009301 11.67005678108389 3 1.8723237023468924 4.389638520973218 4 0.7188576069983443 2.247136389089998 5 0.3521763268424715 1.3761213481628936 6 0.20812305873038045 0.9758835980770872 7 0.1353382784845034 0.740363937242222 8 0.09554335132353754 0.5973337122805901 9 0.07721461714269806 0.5430859910072147 >10 0.37980801602235337 5.4802908726616 >50 0.02966993994526985 1.5863542784907148 >100 0.008221895991536655 0.9339966427653424 >500 0.0 0.0 >1k 1.3478505977589786E-4 0.17538100798745265 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.7870671780119224E-5 0.0 0.0 0.0 0.0 3 5.7870671780119224E-5 0.0 0.0 0.0 0.0 4 5.7870671780119224E-5 0.0 0.0 0.0 0.0 5 5.7870671780119224E-5 0.0 0.0 0.0 0.0 6 5.7870671780119224E-5 0.0 0.0 0.0 0.0 7 3.4722403068071533E-4 0.0 0.0 0.0 0.0 8 3.4722403068071533E-4 0.0 0.0 0.0 0.0 9 3.4722403068071533E-4 0.0 0.0 5.7870671780119224E-5 0.0 10-11 4.0509470246083456E-4 5.7870671780119224E-5 0.0 1.7361201534035766E-4 0.0 12-13 4.3403003835089415E-4 1.1574134356023845E-4 0.0 1.7361201534035766E-4 0.0 14-15 4.629653742409538E-4 1.7361201534035766E-4 0.0 1.7361201534035766E-4 0.0 16-17 4.629653742409538E-4 1.7361201534035766E-4 0.0 2.0254735123041728E-4 0.0 18-19 5.20836046021073E-4 1.7361201534035766E-4 0.0 2.893533589005961E-4 0.0 20-21 5.787067178011923E-4 1.7361201534035766E-4 0.0 4.919007101310134E-4 0.0 22-23 5.787067178011923E-4 1.7361201534035766E-4 0.0 8.101894049216691E-4 0.0 24-25 8.101894049216691E-4 1.7361201534035766E-4 0.0 0.0011574134356023844 0.0 26-27 8.680600767017883E-4 1.7361201534035766E-4 0.0 0.002025473512304173 0.0 28-29 0.0011284780997123248 1.7361201534035766E-4 0.0 0.005555584490891446 0.0 30-31 0.0013310254509427421 1.7361201534035766E-4 0.0 0.015133180670501178 0.0 32-33 0.0013599607868328017 1.7361201534035766E-4 0.0 0.03576407516011368 0.0 34-35 0.0014757021303930403 1.7361201534035766E-4 0.0 0.07173069767145777 0.0 36-37 0.0017361201534035768 1.7361201534035766E-4 0.0 0.12682357720613127 0.0 38-39 0.0021122795199743517 1.7361201534035766E-4 0.0 0.20367582933012962 0.0 40-41 0.0021412148558644113 1.7361201534035766E-4 0.0 0.27728732383444127 0.0 42-43 0.002285891535314709 1.7361201534035766E-4 0.0 0.3521430377820255 0.0 44-45 0.0023726975429848884 1.7361201534035766E-4 0.0 0.44684839215019057 0.0 46-47 0.0024305682147650076 1.7361201534035766E-4 0.0 0.5638628904895917 0.0 48-49 0.002546309558325246 2.0254735123041728E-4 0.0 0.6887478001910889 0.0 50-51 0.002546309558325246 2.314826871204769E-4 0.0 0.8142982226180576 0.0 52-53 0.002546309558325246 2.314826871204769E-4 0.0 0.9395592916861257 0.0 54-55 0.002546309558325246 2.314826871204769E-4 0.0 1.07758084388171 0.0 56-57 0.0027777922454457226 2.314826871204769E-4 0.0 1.2473444595486898 0.0 58-59 0.0027777922454457226 2.604180230105365E-4 0.0 1.4300711056944162 0.0 60-61 0.0028356629172258422 2.8935335890059615E-4 0.0 1.627467967136403 0.0 62-63 0.0028356629172258422 2.8935335890059615E-4 0.0 1.8257328886550914 0.0 64-65 0.0028356629172258422 3.1828869479065574E-4 0.0 2.0350221731478926 0.0 66-67 0.0029514042607860806 3.4722403068071533E-4 0.0 2.2807699808621686 0.0 68-69 0.0029514042607860806 3.4722403068071533E-4 0.0 2.5604589375754854 0.0 70-71 0.0030092749325661998 3.4722403068071533E-4 0.0 2.875854098777135 0.0 72-73 0.0030092749325661998 3.4722403068071533E-4 0.0 3.1946636296138116 0.0 74-75 0.0030092749325661998 3.4722403068071533E-4 0.0 3.5145148325425306 0.0 76-77 0.0030382102684562593 3.4722403068071533E-4 0.0 3.8700143692878033 0.0 78-79 0.003067145604346319 3.4722403068071533E-4 0.0 4.269900711288427 0.0 80-81 0.003645852322147511 3.4722403068071533E-4 0.0 4.705522193113275 0.0 82-83 0.0038773350092679883 3.4722403068071533E-4 0.0 5.155437730867812 0.0 84-85 0.0039352056810481074 3.4722403068071533E-4 0.0 5.606857906088631 0.0 86-87 0.003993076352828227 3.4722403068071533E-4 0.0 6.085477297046108 0.0 88-89 0.003993076352828227 3.4722403068071533E-4 0.0 6.609698777366317 0.0 90-91 0.004022011688718286 3.4722403068071533E-4 0.0 7.186958728373007 0.0 92-93 0.004108817696388465 3.4722403068071533E-4 0.0 7.7808564975164805 0.0 94-95 0.004108817696388465 3.4722403068071533E-4 0.0 8.380859622532757 0.0 96-97 0.004108817696388465 3.4722403068071533E-4 0.0 8.997905660388277 0.0 98-99 0.0044271063911791204 3.4722403068071533E-4 0.0 9.650310678701452 0.0 100-101 0.004687524414189657 3.4722403068071533E-4 0.0 10.368369974149171 0.0 102-103 0.004861136429530015 3.4722403068071533E-4 0.0 11.114496545410248 0.0 104-105 0.0053241018037709685 3.4722403068071533E-4 0.0 11.869564135461353 0.0 106-107 0.005410907811441147 3.4722403068071533E-4 0.0 12.606142045878713 0.0 108-109 0.0054687784832212665 3.4722403068071533E-4 0.0 13.37249441692694 0.0 110-111 0.005497713819111326 3.4722403068071533E-4 0.0 14.17845926280866 0.0 112-113 0.005526649155001386 3.4722403068071533E-4 0.0 15.017265714925598 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1740 0.0 54.02533 1 GTATCAA 2860 0.0 43.89409 1 ATCAACG 3055 0.0 40.30867 3 TCAACGC 3100 0.0 39.532784 4 CAACGCA 3155 0.0 38.843624 5 AACGCAG 3315 0.0 36.594006 6 GTACATG 7415 0.0 35.625534 1 TATCAAC 3670 0.0 33.87912 2 TACATGG 7705 0.0 33.664 2 ACATGGG 7530 0.0 33.181316 3 ACGCAGA 4215 0.0 28.639257 7 CATGGGG 4270 0.0 28.421968 4 CGCAGAG 4370 0.0 27.487373 8 TAGTACG 150 1.6887888E-4 23.796434 4 CATGGGA 3485 0.0 23.045326 4 GAGTACA 5285 0.0 22.515137 1 GCAGAGT 5250 0.0 22.200361 9 GTGGTAT 1185 0.0 21.087278 1 GTGTAGC 1190 0.0 20.998676 1 CAGAGTA 4680 0.0 20.901785 10-11 >>END_MODULE