FastQCFastQC Report
Thu 26 May 2016
SRR1364192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727991
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44570.25792958412399136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.22824192950079022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20260.11724598102652155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.073.145721
GTATCAA51850.057.073431
ATCAACG63800.046.1712843
CAACGCA64750.045.4820185
TCAACGC64800.045.458764
TATCAAC67050.043.9358442
AACGCAG67850.043.4891556
TGGTATC12150.037.22632
ACGCAGA79600.036.994857
CGCAGAG82300.035.7088858
GTGGTAT13800.034.0858651
GTACATG73200.031.31671
GCAGAGT94900.030.4662659
TACATGG74350.030.0967392
ACATGGG73650.029.8962273
GAGTACT55600.026.58969512-13
CAGAGTA90100.026.34494210-11
CATGGGG42300.024.7605174
GTACTTT58400.024.75455914-15
AGAGTAC84500.024.46511510-11
Produced by FastQC (version 0.11.5)