FastQCFastQC Report
Thu 26 May 2016
SRR1364191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15950.051.4800871
GTATCAA28850.044.5480651
ATCAACG29100.043.1332473
TCAACGC30950.040.938394
CAACGCA31700.039.408845
AACGCAG32550.038.3672036
GTACATG75500.037.4341161
TATCAAC35650.035.3778762
TACATGG80500.034.5864032
ACATGGG78450.033.6676453
ACGCAGA40700.030.2452267
CGCAGAG42350.028.5051638
CATGGGG42000.027.4766794
CATGGGA41200.026.8551484
GTGGTAT10000.023.8002171
GCAGAGT50650.023.6009929
GAGTACA55300.023.4559081
AGTACAT54550.022.4661122
ATGGGAG22550.021.3684045
TGGTATC10850.020.8357582
Produced by FastQC (version 0.11.5)