##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991488 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.38565434489186 31.0 31.0 34.0 26.0 34.0 2 30.340523267024455 31.0 31.0 34.0 26.0 34.0 3 30.462965882797185 31.0 31.0 34.0 26.0 34.0 4 33.844972201690396 37.0 35.0 37.0 28.0 37.0 5 33.90906347414597 37.0 35.0 37.0 30.0 37.0 6 33.97616053925507 37.0 35.0 37.0 30.0 37.0 7 33.83249208631938 37.0 35.0 37.0 29.0 37.0 8 33.76613918838577 37.0 35.0 37.0 28.0 37.0 9 35.169933235851786 39.0 35.0 39.0 28.0 39.0 10-11 35.12103110839734 39.0 35.0 39.0 28.0 39.0 12-13 35.09923333708262 39.0 35.0 39.0 27.0 39.0 14-15 36.32417820242954 40.0 36.5 41.0 27.0 41.0 16-17 36.342810501494355 40.0 36.5 41.0 27.0 41.0 18-19 36.218525293649776 40.0 36.0 41.0 27.0 41.0 20-21 36.17945300197641 40.0 36.0 41.0 27.0 41.0 22-23 36.2028915062506 40.0 36.0 41.0 27.0 41.0 24-25 36.057852470112806 40.0 36.0 41.0 27.0 41.0 26-27 36.05500685919272 40.0 36.0 41.0 27.0 41.0 28-29 36.134954114712215 40.0 36.0 41.0 27.0 41.0 30-31 36.02550429628499 40.0 36.0 41.0 27.0 41.0 32-33 35.84677110783495 40.0 35.5 41.0 26.5 41.0 34-35 35.79467237563068 40.0 35.0 41.0 26.5 41.0 36-37 35.811287338914426 40.0 35.0 41.0 27.0 41.0 38-39 35.64719797457981 40.0 35.0 41.0 26.0 41.0 40-41 35.63847384468298 40.0 35.0 41.0 26.0 41.0 42-43 35.55420193342867 40.0 35.0 41.0 26.0 41.0 44-45 35.53298438152778 40.0 35.0 41.0 26.5 41.0 46-47 35.44396677258412 39.5 35.0 41.0 26.5 41.0 48-49 35.34297570459877 39.0 35.0 41.0 26.0 41.0 50-51 35.21261739965293 39.0 35.0 41.0 26.0 41.0 52-53 35.057339537069765 39.0 34.5 41.0 26.0 41.0 54-55 34.856503780088055 39.0 34.0 41.0 26.0 41.0 56-57 34.65998740640165 38.5 34.0 40.5 25.0 41.0 58-59 34.4287823978854 38.0 34.0 40.0 24.5 41.0 60-61 34.18351805283285 38.0 33.5 40.0 24.0 41.0 62-63 33.88900083756468 37.0 33.0 40.0 23.0 41.0 64-65 33.58597867524183 37.0 33.0 40.0 23.0 41.0 66-67 33.2920218449722 36.0 33.0 39.5 22.0 41.0 68-69 33.07848327481763 36.0 33.0 39.0 22.0 41.0 70-71 32.92279190233634 35.5 33.0 39.0 22.0 41.0 72-73 32.62166756716586 35.0 33.0 38.5 22.0 40.0 74-75 32.2981077465694 35.0 32.0 37.0 21.5 39.5 76-77 31.97600286820709 35.0 32.0 37.0 20.0 39.0 78-79 31.64379474041521 35.0 32.0 36.5 20.0 39.0 80-81 31.34989666018575 35.0 32.0 36.0 19.0 38.0 82-83 31.086463739676063 35.0 32.0 36.0 18.0 37.0 84-85 30.846738971060837 35.0 32.0 35.0 17.5 37.0 86-87 30.638528577626378 35.0 31.0 35.0 15.5 36.0 88-89 30.467200655991903 35.0 31.0 35.0 12.5 36.0 90-91 30.32372502370087 35.0 31.0 35.0 10.5 36.0 92-93 30.1944741821191 35.0 31.0 35.0 7.5 36.0 94-95 30.06770942129704 35.0 31.0 35.0 7.0 35.0 96-97 29.974107802808753 35.0 31.0 35.0 6.0 35.0 98-99 29.897715677925248 35.0 31.0 35.0 2.0 35.0 100-101 29.793549848153745 35.0 31.0 35.0 2.0 35.0 102-103 28.810239629864704 33.5 29.0 34.5 2.0 35.0 104-105 29.5070015485908 34.0 30.0 35.0 2.0 35.0 106-107 29.622505131841116 34.0 31.0 35.0 2.0 35.0 108-109 29.590658090834594 34.0 31.0 35.0 2.0 35.0 110-111 29.525838719590578 34.0 31.0 35.0 2.0 35.0 112-113 29.68716055532346 35.0 31.0 35.0 2.0 35.0 114-115 29.682333260356074 35.0 31.0 35.0 2.0 35.0 116-117 29.594618948243728 35.0 31.0 35.0 2.0 35.0 118-119 29.35088612133239 35.0 31.0 35.0 2.0 35.0 120-121 29.28730075200051 35.0 31.0 35.0 2.0 35.0 122-123 29.151516604669474 35.0 31.0 35.0 2.0 35.0 124-125 28.26307288821223 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0250468775668828 1101 2 1.0500312029221703 1101 3 1.1183626888042504 1101 4 1.1206851963878606 1101 5 0.9197973400341226 1101 6 0.9982284365192484 1101 7 1.0665885766724799 1101 8 0.9819491209561235 1101 9 1.3213300837348285 1101 10-11 1.3106854037897975 1101 12-13 1.2504419560441065 1101 14-15 1.4639288167607631 1101 16-17 1.4826476786709932 1101 18-19 1.5323928777215627 1101 20-21 1.5596652085530351 1101 22-23 1.5405847007154563 1101 24-25 1.7447694259846855 1101 26-27 1.7773220617128516 1101 28-29 1.6443903875442416 1101 30-31 1.7475753359870367 1101 32-33 1.87719133787769 1101 34-35 1.8174448387980107 1101 36-37 1.7352607500333121 1101 38-39 1.7934916205719134 1101 40-41 1.6767863830725034 1101 42-43 1.6271262209195498 1101 44-45 1.5686763210822292 1101 46-47 1.602033885374091 1101 48-49 1.6195139648618166 1101 50-51 1.5368453152185069 1101 52-53 1.431397160415976 1101 54-55 1.4958759440858245 1101 56-57 1.5388782880407348 1101 58-59 1.5265221036834618 1101 60-61 1.4812481528301547 1101 62-63 1.4234509252315135 1101 64-65 1.3954236123432295 1101 66-67 1.3670844291285107 1101 68-69 1.3594630660469988 1101 70-71 1.4029176569131323 1101 72-73 1.3568040839963373 1101 74-75 1.363482979827495 1101 76-77 1.3816516988231626 1101 78-79 1.3221734436409172 1101 80-81 1.2995901938310759 1101 82-83 1.337518299895656 1101 84-85 1.3750749405826745 1101 86-87 1.3597169937053515 1101 88-89 1.3346794368519603 1101 90-91 1.4136780468378944 1101 92-93 1.3772583497965094 1101 94-95 1.4290561042802565 1101 96-97 1.469013847737802 1101 98-99 1.4341859180123713 1101 100-101 1.3538676779038923 1101 102-103 1.2642207192503072 1101 104-105 1.3612317700357117 1101 106-107 1.3327868264160827 1101 108-109 1.3210567550914547 1101 110-111 1.3336012197101574 1101 112-113 1.5136860006500115 1101 114-115 1.4861896657244245 1101 116-117 1.489791396698969 1101 118-119 1.2890515569156769 1101 120-121 1.2933094510408907 1101 122-123 1.429472203731116 1101 124-125 1.3529809039468255 1102 1 0.26931079633688526 1102 2 0.3246146164243555 1102 3 0.27546885568373014 1102 4 0.26607760104523237 1102 5 0.2454956835999127 1102 6 0.24388583566683764 1102 7 0.2834544264914456 1102 8 0.25652123793851445 1102 9 0.39246135366771995 1102 10-11 0.39250409246062645 1102 12-13 0.4207858221067369 1102 14-15 0.46610667645652626 1102 16-17 0.4203706938360199 1102 18-19 0.5188490792922948 1102 20-21 0.5910733598302045 1102 22-23 0.5758167239440155 1102 24-25 0.7651621950459315 1102 26-27 0.7907758523876112 1102 28-29 0.5831873008977482 1102 30-31 0.7297796732675934 1102 32-33 0.8456742298450592 1102 34-35 0.7306857447351689 1102 36-37 0.6328379380598719 1102 38-39 0.7521458115078801 1102 40-41 0.6658048904159699 1102 42-43 0.6810430522967721 1102 44-45 0.5097933938405674 1102 46-47 0.517701538846417 1102 48-49 0.45910994300924557 1102 50-51 0.34732761102115717 1102 52-53 0.3577307327982382 1102 54-55 0.38322978169580324 1102 56-57 0.3861234677064118 1102 58-59 0.460503055564665 1102 60-61 0.4323757641482402 1102 62-63 0.4606004415947069 1102 64-65 0.4238491179900379 1102 66-67 0.49412782379830844 1102 68-69 0.44695552288700213 1102 70-71 0.45500372327544625 1102 72-73 0.4175766274770041 1102 74-75 0.4436219488852373 1102 76-77 0.3482342246375367 1102 78-79 0.6072070489652965 1102 80-81 0.5201347006614618 1102 82-83 0.46163953997658425 1102 84-85 0.437110143453598 1102 86-87 0.4644187874469381 1102 88-89 0.3812136281377505 1102 90-91 0.4297919857229964 1102 92-93 0.4560481135461991 1102 94-95 0.4525927676818675 1102 96-97 0.4544473498630026 1102 98-99 0.4379017442179638 1102 100-101 0.4406599327183969 1102 102-103 0.36494777784061583 1102 104-105 0.41325443012562246 1102 106-107 0.4698970600426904 1102 108-109 0.3744193281908679 1102 110-111 0.32685045591966144 1102 112-113 0.3831725701811841 1102 114-115 0.44397967289589246 1102 116-117 0.3634997672014961 1102 118-119 0.30248239014698086 1102 120-121 0.33580313227788494 1102 122-123 0.3706916739753545 1102 124-125 0.26592099449389295 1103 1 0.29190357761995145 1103 2 0.09622874730877484 1103 3 0.37055143679523184 1103 4 0.3569342412807188 1103 5 0.19078052638446508 1103 6 0.26172579421299247 1103 7 0.373819020030389 1103 8 0.40522602298377564 1103 9 0.5572055547014543 1103 10-11 0.4803899463358192 1103 12-13 0.40827999810269233 1103 14-15 0.5399060607449968 1103 16-17 0.44557611244584194 1103 18-19 0.5712193945069828 1103 20-21 0.6991015588957481 1103 22-23 0.7012167840346777 1103 24-25 0.9982910895084416 1103 26-27 1.0200434866009687 1103 28-29 0.9109306789507201 1103 30-31 0.9011643275068124 1103 32-33 1.2010179440573694 1103 34-35 1.0257482956271673 1103 36-37 0.7749283962729194 1103 38-39 1.034940941551902 1103 40-41 0.9202376650803501 1103 42-43 0.8842888187356976 1103 44-45 0.7876332217548523 1103 46-47 0.7894702507726734 1103 48-49 0.6850366672410217 1103 50-51 0.644039389225668 1103 52-53 0.5733016158261179 1103 54-55 0.570565675413711 1103 56-57 0.6685256135525321 1103 58-59 0.7578081201621245 1103 60-61 0.623720967563834 1103 62-63 0.6857934501772149 1103 64-65 0.5422483986438849 1103 66-67 0.5135582253171194 1103 68-69 0.612684905912694 1103 70-71 0.6227285080594456 1103 72-73 0.5818570853852449 1103 74-75 0.6292008440009127 1103 76-77 0.6810983718509362 1103 78-79 0.5804046557895042 1103 80-81 0.7186592137311827 1103 82-83 0.6176292287679317 1103 84-85 0.585577613941684 1103 86-87 0.692384457392464 1103 88-89 0.7311894612424794 1103 90-91 0.7113722394100819 1103 92-93 0.7338411317083811 1103 94-95 0.6565004022435765 1103 96-97 0.7697306250651579 1103 98-99 0.7409317503576638 1103 100-101 0.7412177307876604 1103 102-103 0.6567292916897713 1103 104-105 0.6959711810498383 1103 106-107 0.7417408852920886 1103 108-109 0.6473669927108148 1103 110-111 0.7375711954701991 1103 112-113 0.8550986935063776 1103 114-115 0.8167703614564594 1103 116-117 0.8648877307813301 1103 118-119 0.7153890362608983 1103 120-121 0.7414771273922511 1103 122-123 0.8399485528317143 1103 124-125 0.7310216028029508 1104 1 0.496436676887555 1104 2 0.5667150228797162 1104 3 0.5379326562744282 1104 4 0.5530302308172992 1104 5 0.48340344377042044 1104 6 0.5061476572778503 1104 7 0.4300428288929581 1104 8 0.5894675312099196 1104 9 0.6963372900071718 1104 10-11 0.6758441756897753 1104 12-13 0.7595543464851389 1104 14-15 0.7994455915451439 1104 16-17 0.8166717509284069 1104 18-19 0.9495095229348038 1104 20-21 0.9590212867720496 1104 22-23 0.9379990429458189 1104 24-25 1.1372473535269236 1104 26-27 1.122277104024306 1104 28-29 1.0200632879908937 1104 30-31 1.0390689339819446 1104 32-33 1.0895661495695208 1104 34-35 1.152822272627887 1104 36-37 1.0389869953244286 1104 38-39 1.1077073631345726 1104 40-41 1.038979124158935 1104 42-43 0.9763760834097042 1104 44-45 0.8807066138884352 1104 46-47 0.9087130484258026 1104 48-49 0.919392474198311 1104 50-51 0.8739471745785394 1104 52-53 0.9440957936341974 1104 54-55 0.8660082252561381 1104 56-57 0.8380568438754068 1104 58-59 0.9153543662356043 1104 60-61 0.8167792810914065 1104 62-63 0.8893458828255518 1104 64-65 0.8961233886416338 1104 66-67 0.9223737267036185 1104 68-69 0.8888551472751516 1104 70-71 0.783109598930281 1104 72-73 0.8409973618909916 1104 74-75 0.7900865053722015 1104 76-77 0.8207586384870567 1104 78-79 0.8133725177412465 1104 80-81 0.8286803795024547 1104 82-83 0.7590318935242735 1104 84-85 0.7850238847338318 1104 86-87 0.7645471217832629 1104 88-89 0.8208605826917896 1104 90-91 0.8585621132735852 1104 92-93 0.7775062337514278 1104 94-95 0.7829029187771042 1104 96-97 0.830661511109902 1104 98-99 0.8137661538299525 1104 100-101 0.7504167949110752 1104 102-103 0.6609475122181188 1104 104-105 0.7736220456791756 1104 106-107 0.7006912471451976 1104 108-109 0.7570024448427617 1104 110-111 0.7298673428585012 1104 112-113 0.7697997548485525 1104 114-115 0.8095838956030939 1104 116-117 0.7414792592571615 1104 118-119 0.7879756958960833 1104 120-121 0.8818432912232552 1104 122-123 0.8981490187194083 1104 124-125 0.8579321446542743 1105 1 0.4877216156820232 1105 2 0.45898434524406895 1105 3 0.4752478744505275 1105 4 0.544916067084614 1105 5 0.45561050215670207 1105 6 0.3534874016664844 1105 7 0.3712246204474141 1105 8 0.3491492650150292 1105 9 0.37480342987340265 1105 10-11 0.4647689624004272 1105 12-13 0.49329199546763647 1105 14-15 0.49160253964597445 1105 16-17 0.5226147615078247 1105 18-19 0.6725839039782215 1105 20-21 0.7566044385196662 1105 22-23 0.6412880994384906 1105 24-25 0.8978164496565881 1105 26-27 0.9523062033767076 1105 28-29 0.863946087356311 1105 30-31 0.8419357707722241 1105 32-33 1.0877183459580237 1105 34-35 1.0679187362731213 1105 36-37 0.8833518583976172 1105 38-39 0.9446951126664587 1105 40-41 0.8861699606538593 1105 42-43 0.930716651969874 1105 44-45 0.7819179576625217 1105 46-47 0.7017066775083478 1105 48-49 0.6390011922318237 1105 50-51 0.6255497336177385 1105 52-53 0.6505460272118739 1105 54-55 0.6864647996771396 1105 56-57 0.6076712315257708 1105 58-59 0.6262806716312497 1105 60-61 0.6081785717866453 1105 62-63 0.6704385824068879 1105 64-65 0.6475494815875393 1105 66-67 0.7545322967010293 1105 68-69 0.7189113367473112 1105 70-71 0.7252713155943127 1105 72-73 0.6020645534623696 1105 74-75 0.5738527013728998 1105 76-77 0.5486709170516519 1105 78-79 0.4960221210260407 1105 80-81 0.4772761809902697 1105 82-83 0.44735228386539916 1105 84-85 0.4880239019357653 1105 86-87 0.5075772669430059 1105 88-89 0.5368196823449303 1105 90-91 0.5000941221516548 1105 92-93 0.4546053464944215 1105 94-95 0.5889902032599892 1105 96-97 0.5549165649970078 1105 98-99 0.5309710479321588 1105 100-101 0.6420418053989891 1105 102-103 0.5258061971642363 1105 104-105 0.5072977911050707 1105 106-107 0.5526289396357136 1105 108-109 0.5762596232271626 1105 110-111 0.5812945357919936 1105 112-113 0.5177276532368467 1105 114-115 0.5907179617387968 1105 116-117 0.5976449412594107 1105 118-119 0.39817210101963596 1105 120-121 0.4970207092738903 1105 122-123 0.5388198777430091 1105 124-125 0.5357406323691087 1106 1 0.42562818273623293 1106 2 0.4488244101004142 1106 3 0.4698498523828434 1106 4 0.6059450504916555 1106 5 0.49581189205193965 1106 6 0.4704361270914603 1106 7 0.4457975457165375 1106 8 0.45060952217067296 1106 9 0.5158489881972628 1106 10-11 0.590075391757118 1106 12-13 0.6069136322523079 1106 14-15 0.7553088260672851 1106 16-17 0.6820252461902925 1106 18-19 0.7317946516012555 1106 20-21 0.756091186442994 1106 22-23 0.739710921609074 1106 24-25 0.9108337537999134 1106 26-27 0.8809873277008009 1106 28-29 0.7908720048111348 1106 30-31 0.8738997529192645 1106 32-33 1.0641406431786464 1106 34-35 1.0816174277975108 1106 36-37 0.9736276550033693 1106 38-39 0.9711499137179871 1106 40-41 0.9209606994190835 1106 42-43 0.9521949622828174 1106 44-45 0.8341943606960598 1106 46-47 0.8141662659638769 1106 48-49 0.8062585149055295 1106 50-51 0.8037070428475985 1106 52-53 0.7503293544052738 1106 54-55 0.7646080830820381 1106 56-57 0.5999428273678404 1106 58-59 0.7001806482936672 1106 60-61 0.6375145450013946 1106 62-63 0.641092191670694 1106 64-65 0.6274347026165046 1106 66-67 0.6420018807393006 1106 68-69 0.5863334774141151 1106 70-71 0.6452899507360428 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scores pass #Quality Count 2 54877.0 3 10161.0 4 6569.0 5 5418.0 6 5431.0 7 6130.0 8 6357.0 9 6927.0 10 7762.0 11 8416.0 12 8876.0 13 8820.0 14 8559.0 15 8483.0 16 7972.0 17 7476.0 18 7014.0 19 6519.0 20 6390.0 21 6623.0 22 7477.0 23 10241.0 24 15450.0 25 21896.0 26 24878.0 27 25131.0 28 28647.0 29 33966.0 30 40065.0 31 47744.0 32 60631.0 33 79595.0 34 110902.0 35 173508.0 36 336300.0 37 568515.0 38 211080.0 39 682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.108109085866225 18.19710663989565 11.972543628517968 24.722240645720152 2 16.340231460362563 20.215152836585126 38.449287914746705 24.995327788305605 3 19.107538554822725 24.39060045503708 29.000450616319544 27.50141037382065 4 12.6458475459232 16.24264168800858 36.341094119077304 34.77041664699092 5 13.915709012432256 38.21587961186118 33.742668142917715 14.125743232788848 6 33.156622416123824 36.19631131652897 17.039070236438103 13.607996030909094 7 28.957588283676746 32.46101639084137 20.592595047497387 17.98880027798449 8 26.234855545200375 35.75135777870617 19.52926655847286 18.484520117620594 9 26.528293170371636 16.277824923297864 19.11040588108282 38.083476025247684 10-11 25.127566232377806 26.836073719385173 27.51506277064592 20.521297277591103 12-13 25.331538647141045 24.366028609025303 27.368663142071707 22.93376960176195 14-15 22.37188576312629 26.032299704944233 24.69419131093293 26.90162322099654 16-17 23.290597485863724 28.936695658549088 25.209254001982 22.563452853605177 18-19 23.013192613462174 27.67568913911186 26.71502342973803 22.59609481768794 20-21 23.68924028288958 27.112268474926264 26.50804220544597 22.690449036738183 22-23 23.492681623502374 27.05054661823909 26.292266989160044 23.164504769098492 24-25 23.332525700914047 27.126958560924567 26.595979999171444 22.94453573898994 26-27 23.251243748612616 27.365608354535404 26.279205366927183 23.103942529924797 28-29 23.05463246712975 27.28700719736963 26.617587795229625 23.040772540270996 30-31 23.16986515720946 27.070925540184675 26.56744951681166 23.191759785794204 32-33 22.88172531123332 27.179537137095316 26.715197907837172 23.223539643834183 34-35 23.57980898216735 27.18470933237173 26.255359412753503 22.980122272707412 36-37 23.5183717582869 27.01709661834293 26.138184188666475 23.326347434703695 38-39 23.13372188804642 27.114398624186826 26.555851224128162 23.196028263638592 40-41 23.364250150022723 26.937301800523763 26.60509248681181 23.093355562641705 42-43 23.300910665133788 27.05808537620581 26.43914140228521 23.201862556375197 44-45 23.258063649535636 27.246848894304172 26.23850511440341 23.256582341756786 46-47 23.267462089532902 27.206429733727255 26.071645303099196 23.454462873640647 48-49 23.362479239034506 27.16710584294855 26.16179178901461 23.30862312900233 50-51 23.02779745922676 27.149009184806378 26.22010895627839 23.603084399688466 52-53 23.462181979233304 26.97706906196365 26.215943293807854 23.344805664995192 54-55 23.19484774871476 26.81420384284629 26.349396455944106 23.641551952494837 56-57 23.15311318450025 27.29918844566367 26.252551250348056 23.295147119488025 58-59 23.372157576527584 26.915778920010275 26.165800701995078 23.546262801467062 60-61 23.309207706306 27.012725404026618 26.41569083975312 23.26237604991426 62-63 23.221701691386233 27.02574505840969 26.363091476075297 23.38946177412879 64-65 23.0112125626498 26.920695561524475 26.317535114267304 23.75055676155842 66-67 22.98081003110869 27.342554628516048 26.118243149936855 23.55839219043841 68-69 23.030360401711867 26.897725983011917 26.486055688859118 23.585857926417106 70-71 22.878913910723327 26.92867492518599 26.498006340642704 23.694404823447975 72-73 23.006891821081304 26.8054655513143 26.53194192983544 23.655700697768953 74-75 22.81445498302632 26.947592112441814 26.415145027619108 23.822807876912755 76-77 22.927534834628098 26.913990141005613 26.501407746295346 23.657067278070944 78-79 22.98871209180571 26.980154392110567 26.460048919792527 23.571084596291193 80-81 22.908847228739727 27.05234902631599 26.519290269336405 23.519513475607873 82-83 23.036382631121864 26.927751695553788 26.47002184579794 23.565843827526407 84-85 23.03152260623773 26.750009478953835 26.35488148545627 23.863586429352164 86-87 23.036653026933557 26.603154070063052 26.640440080377086 23.719752822626305 88-89 22.964643108126463 26.655821974987575 26.580474885014716 23.799060031871242 90-91 22.91668026631544 26.89082402824442 26.67151399993874 23.520981705501406 92-93 22.973769965781017 26.459491468102076 26.761911050730415 23.80482751538649 94-95 22.86644458816724 26.4441965003053 26.694587162965583 23.99477174856188 96-97 22.872684982932068 26.504930568318425 26.645380711781442 23.977003736968065 98-99 22.72777727272499 26.4893462602292 26.588166395528656 24.19471007151715 100-101 22.530002764676325 26.45986084211388 26.867079334157463 24.14305705905233 102-103 22.88614039259267 26.36219672920627 26.747136304163277 24.004526574037783 104-105 22.608315283087947 26.47505199123544 26.789058462706677 24.127574262969937 106-107 22.461191642424396 26.57430366721995 26.912653223600785 24.05185146675487 108-109 22.621086383586317 26.607984441569393 26.541651215276424 24.229277959567867 110-111 22.29927093433789 26.766185292848988 26.83312134406386 24.10142242874926 112-113 22.490148323199787 26.629522461421328 26.923145495449006 23.95718371992988 114-115 22.20422045117835 26.779871045257725 26.75744347838754 24.25846502517639 116-117 22.226612552565694 26.672814245569736 26.95902582560888 24.141547376255694 118-119 22.213714300112123 26.726633659624326 26.565932196984015 24.49371984327954 120-121 22.142797713921457 26.78176657053782 26.834024534347357 24.241411181193367 122-123 22.238692593480796 26.728307093880698 26.82488602505879 24.20811428757971 124-125 22.187786419890838 26.75491411212762 26.720509093528506 24.33679037445304 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1092.0 1 909.5 2 657.0 3 503.5 4 369.0 5 306.0 6 280.5 7 265.5 8 289.0 9 288.5 10 270.0 11 266.5 12 281.0 13 298.0 14 321.0 15 403.0 16 481.0 17 544.0 18 695.5 19 958.0 20 1235.5 21 1678.5 22 2349.0 23 3252.0 24 4412.5 25 5864.5 26 8055.5 27 10576.5 28 13001.0 29 15824.5 30 19010.0 31 22444.0 32 26537.0 33 30653.5 34 34974.0 35 38539.0 36 41885.0 37 46272.5 38 49537.0 39 51216.0 40 53285.0 41 57875.5 42 62969.0 43 67145.0 44 70046.0 45 72585.5 46 73116.0 47 73161.5 48 76865.5 49 78907.5 50 78353.0 51 76784.5 52 74610.5 53 74053.5 54 73405.5 55 69940.0 56 64472.5 57 59204.5 58 54519.0 59 48374.5 60 40559.0 61 32711.5 62 27159.5 63 22140.0 64 17316.5 65 13933.5 66 10518.0 67 8572.0 68 6361.5 69 3864.5 70 2862.5 71 2324.0 72 1952.5 73 1589.0 74 1206.0 75 957.0 76 650.5 77 344.5 78 186.5 79 102.5 80 59.0 81 42.0 82 25.5 83 20.5 84 18.5 85 14.5 86 15.0 87 11.5 88 8.5 89 9.0 90 5.5 91 3.0 92 2.0 93 3.0 94 3.5 95 1.5 96 2.5 97 2.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06407269338303821 2 0.05001285470964424 3 0.04423827811164315 4 0.04428849182119099 5 0.043183790211138606 6 0.0050213709547835585 7 5.02137095478356E-4 8 0.0 9 9.038467718610406E-4 10-11 0.0035902802326702446 12-13 0.002686433460809204 14-15 0.008511223768358132 16-17 0.003439639104026738 18-19 0.0054481874859401615 20-21 0.009088681428158241 22-23 0.01687180640807276 24-25 0.003540066523122409 26-27 0.016570524150785745 28-29 0.006803957643731722 30-31 0.0066282096603142975 32-33 0.002811967734678793 34-35 0.01832800398495999 36-37 0.016771378988977084 38-39 0.026111128964874504 40-41 0.006176286274383777 42-43 0.0015817318507568212 44-45 2.2596169296526015E-4 46-47 0.002636219751261368 48-49 0.003540066523122409 50-51 0.002360044348748273 52-53 0.0015064112864350675 54-55 0.006301820548253367 56-57 0.003138356846739724 58-59 0.008209941511071118 60-61 0.015741997943246457 62-63 0.01262874795128065 64-65 0.003063036282417971 66-67 0.004167737892470354 68-69 0.010544879005045473 70-71 0.0024855786226178614 72-73 0.003489852813574573 74-75 0.0011298084648263007 76-77 3.514959668348491E-4 78-79 0.003263891120609313 80-81 0.002837074589452711 82-83 0.0014813044316611499 84-85 0.011775114888967445 86-87 0.00625160683870553 88-89 0.0018830141080438344 90-91 7.532056432175338E-4 92-93 0.0024855786226178614 94-95 0.0 96-97 0.00170726612462641 98-99 0.024956213645274287 100-101 0.014888364880933253 102-103 0.0068541713532795576 104-105 0.014411334640228814 106-107 0.032513376932223545 108-109 0.0013808770125654786 110-111 0.0017574798341742457 112-113 0.016545417296011826 114-115 0.031860598708101685 116-117 0.05033924382170518 118-119 0.3546594305363628 120-121 0.11637027187710897 122-123 0.018604179387473087 124-125 0.3169740415207122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1991488.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.69190159386055 #Duplication Level Percentage of deduplicated Percentage of total 1 88.04856831111275 70.16757841329506 2 8.099212081042758 12.908832243005323 3 1.978731062239534 4.730665232781242 4 0.7273465294749727 2.3185451220622206 5 0.3541897740864682 1.411302831102526 6 0.19308631688003075 0.9232449458354632 7 0.1250688159096944 0.6976880238955218 8 0.08985109142126607 0.5728323468515604 9 0.054583139952740736 0.391485079601796 >10 0.30631842701795403 4.204426725061445 >50 0.01819438191856721 0.9979053777011144 >100 0.004737277564481247 0.537249614294898 >500 0.0 0.0 >1k 1.1279137881426683E-4 0.13824404451175493 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.021370954783559E-5 0.0 5 0.0 0.0 0.0 5.021370954783559E-5 0.0 6 0.0 0.0 0.0 5.021370954783559E-5 0.0 7 0.0 0.0 0.0 5.021370954783559E-5 0.0 8 0.0 0.0 0.0 5.021370954783559E-5 0.0 9 0.0 0.0 0.0 5.021370954783559E-5 0.0 10-11 0.0 0.0 0.0 5.021370954783559E-5 0.0 12-13 0.0 0.0 0.0 5.021370954783559E-5 0.0 14-15 0.0 0.0 0.0 5.021370954783559E-5 0.0 16-17 0.0 0.0 0.0 1.0042741909567118E-4 0.0 18-19 0.0 0.0 0.0 1.0042741909567118E-4 0.0 20-21 0.0 0.0 0.0 2.2596169296526015E-4 0.0 22-23 0.0 0.0 0.0 4.770302407044381E-4 0.0 24-25 0.0 0.0 0.0 8.536330623132051E-4 0.0 26-27 0.0 0.0 0.0 0.001230235883921972 0.0 28-29 0.0 0.0 0.0 0.0033392116849310666 0.0 30-31 0.0 0.0 0.0 0.011348298357810843 0.0 32-33 0.0 0.0 0.0 0.027165616865379057 0.0 34-35 0.0 0.0 0.0 0.05199629623678375 0.0 36-37 0.0 0.0 0.0 0.09236811871324356 0.0 38-39 0.0 0.0 0.0 0.1461972153485233 0.0 40-41 0.0 0.0 0.0 0.20128165472249895 0.0 42-43 0.0 0.0 0.0 0.2560145981296397 0.0 44-45 0.0 0.0 0.0 0.3287240495549057 0.0 46-47 0.0 0.0 0.0 0.41757720859980074 0.0 48-49 0.0 0.0 0.0 0.5101461821512356 0.0 50-51 0.0 0.0 0.0 0.6030666516695055 0.0 52-53 0.0 0.0 0.0 0.6933509014365138 0.0 54-55 0.0 0.0 0.0 0.7930502217437414 0.0 56-57 0.0 0.0 0.0 0.9204675097213741 0.0 58-59 0.0 0.0 0.0 1.0621454912105923 0.0 60-61 0.0 0.0 0.0 1.2166279686345085 0.0 62-63 0.0 0.0 0.0 1.3708844843654595 0.0 64-65 0.0 2.5106854773917794E-5 0.0 1.535334383134621 0.0 66-67 0.0 5.021370954783559E-5 0.0 1.7264226548189092 0.0 68-69 0.0 5.021370954783559E-5 0.0 1.946760412314812 0.0 70-71 0.0 1.0042741909567118E-4 0.0 2.1944897483690586 0.0 72-73 0.0 1.0042741909567118E-4 0.0 2.439984574348427 0.0 74-75 0.0 1.0042741909567118E-4 0.0 2.6935135938554486 0.0 76-77 0.0 1.0042741909567118E-4 0.0 2.9778738310248416 0.0 78-79 0.0 1.0042741909567118E-4 0.0 3.2922367596490663 0.0 80-81 0.0 1.0042741909567118E-4 0.0 3.6431301619693413 0.0 82-83 0.0 1.0042741909567118E-4 0.0 4.01134729408362 0.0 84-85 0.0 1.0042741909567118E-4 0.0 4.378309083459202 0.0 86-87 0.0 1.0042741909567118E-4 0.0 4.76696319535945 0.0 88-89 0.0 1.0042741909567118E-4 0.0 5.1984496015040005 0.0 90-91 0.0 1.0042741909567118E-4 0.0 5.668575457145612 0.0 92-93 0.0 1.0042741909567118E-4 0.0 6.163431564739532 0.0 94-95 0.0 1.0042741909567118E-4 0.0 6.665744408201305 0.0 96-97 0.0 1.0042741909567118E-4 0.0 7.174710568178167 0.0 98-99 0.0 1.0042741909567118E-4 0.0 7.707377599061607 0.0 100-101 0.0 1.0042741909567118E-4 0.0 8.298618922132597 0.0 102-103 0.0 1.0042741909567118E-4 0.0 8.921846378185558 0.0 104-105 0.0 1.0042741909567118E-4 0.0 9.538119235466144 0.0 106-107 0.0 1.0042741909567118E-4 0.0 10.163079064498506 0.0 108-109 0.0 1.0042741909567118E-4 0.0 10.796299048751486 0.0 110-111 0.0 1.0042741909567118E-4 0.0 11.487365226403572 0.0 112-113 0.0 1.0042741909567118E-4 0.0 12.227665946267315 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1595 0.0 51.480087 1 GTATCAA 2885 0.0 44.548065 1 ATCAACG 2910 0.0 43.133247 3 TCAACGC 3095 0.0 40.93839 4 CAACGCA 3170 0.0 39.40884 5 AACGCAG 3255 0.0 38.367203 6 GTACATG 7550 0.0 37.434116 1 TATCAAC 3565 0.0 35.377876 2 TACATGG 8050 0.0 34.586403 2 ACATGGG 7845 0.0 33.667645 3 ACGCAGA 4070 0.0 30.245226 7 CGCAGAG 4235 0.0 28.505163 8 CATGGGG 4200 0.0 27.476679 4 CATGGGA 4120 0.0 26.855148 4 GTGGTAT 1000 0.0 23.800217 1 GCAGAGT 5065 0.0 23.600992 9 GAGTACA 5530 0.0 23.455908 1 AGTACAT 5455 0.0 22.466112 2 ATGGGAG 2255 0.0 21.368404 5 TGGTATC 1085 0.0 20.835758 2 >>END_MODULE