FastQCFastQC Report
Thu 26 May 2016
SRR1364191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991488
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.2531273098306392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48770.24489226146479415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.13773620528971303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.070.794211
GTATCAA60500.051.6698041
ATCAACG74300.041.0886043
TCAACGC75300.040.541924
CAACGCA77100.039.5844845
TATCAAC78550.039.0957072
AACGCAG79050.038.6080136
GTGGTAT15250.035.1403051
GTACATG75700.035.0809941
TACATGG77500.033.7890932
ACATGGG77800.032.5853843
ACGCAGA93050.032.4778677
TGGTATC15450.031.9723972
CGCAGAG94950.031.7653168
CATGGGG43250.028.8944974
GCAGAGT106950.027.811829
GAGTACT69650.025.92320312-13
CAGAGTA103650.025.56928410-11
CATGGGA40850.024.182324
AGAGTAC101700.023.7197510-11
Produced by FastQC (version 0.11.5)