FastQCFastQC Report
Thu 26 May 2016
SRR1364190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360214
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24700.053.728991
GTATCAA44150.050.9520721
ATCAACG50350.043.1359063
CAACGCA51300.042.2202035
TCAACGC52550.041.4432564
AACGCAG54950.039.1767736
TATCAAC59200.037.5927932
GTACATG118500.036.861961
TACATGG117500.036.3108182
ACATGGG119400.033.8883253
ACGCAGA66950.031.6198357
CGCAGAG67500.031.0979068
CATGGGG61150.029.6789764
CATGGGA62550.026.3510514
TAAGGTA8450.024.6465914
GCAGAGT86200.023.38839
GGTAAGG11750.022.7887333
ATGGGGG32500.022.1532945
GTGTAAG11650.021.965611
AGGTAAG11450.021.8272252
Produced by FastQC (version 0.11.5)