##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2360214 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.760700512750116 31.0 31.0 34.0 27.0 34.0 2 30.801734503735677 33.0 31.0 34.0 27.0 34.0 3 30.914764932332407 33.0 31.0 34.0 27.0 34.0 4 34.273104896420406 37.0 35.0 37.0 30.0 37.0 5 34.3869526237875 37.0 35.0 37.0 31.0 37.0 6 34.41967508031051 37.0 35.0 37.0 31.0 37.0 7 34.3137656161687 37.0 35.0 37.0 30.0 37.0 8 34.27690370449459 37.0 35.0 37.0 30.0 37.0 9 35.715506729474534 39.0 37.0 39.0 30.0 39.0 10-11 35.67355820277314 39.0 37.0 39.0 30.0 39.0 12-13 35.63009074600862 39.0 37.0 39.0 30.0 39.0 14-15 36.90464847679066 40.0 37.0 41.0 30.0 41.0 16-17 36.937857118040995 40.0 37.5 41.0 30.0 41.0 18-19 36.84303266568201 40.0 37.0 41.0 29.5 41.0 20-21 36.818226440483784 40.0 37.0 41.0 29.0 41.0 22-23 36.86771665620151 40.0 37.0 41.0 29.5 41.0 24-25 36.737616165313824 40.0 37.0 41.0 28.5 41.0 26-27 36.74404630258104 40.0 37.0 41.0 29.5 41.0 28-29 36.83031030237089 40.0 37.0 41.0 30.0 41.0 30-31 36.71606769555642 40.0 37.0 41.0 30.0 41.0 32-33 36.531106289514426 40.0 36.0 41.0 29.5 41.0 34-35 36.48128157023049 40.0 36.0 41.0 29.0 41.0 36-37 36.49608573629341 40.0 36.0 41.0 29.0 41.0 38-39 36.32823782080777 40.0 36.0 41.0 28.5 41.0 40-41 36.31681745807795 40.0 36.0 41.0 29.0 41.0 42-43 36.233872860681274 40.0 36.0 41.0 28.0 41.0 44-45 36.21500211421507 40.0 35.0 41.0 28.5 41.0 46-47 36.11999081439225 40.0 35.0 41.0 28.0 41.0 48-49 36.01313906281379 40.0 35.0 41.0 28.0 41.0 50-51 35.878783449297394 39.5 35.0 41.0 28.0 41.0 52-53 35.71670598513525 39.0 35.0 41.0 28.0 41.0 54-55 35.51091405270878 39.0 35.0 41.0 28.0 41.0 56-57 35.29991136396954 39.0 34.5 41.0 28.0 41.0 58-59 35.05936601511558 38.0 34.0 40.0 27.0 41.0 60-61 34.79490397904597 38.0 34.0 40.0 27.0 41.0 62-63 34.4949729134731 37.0 34.0 40.0 26.0 41.0 64-65 34.169558565452114 37.0 34.0 40.0 26.0 41.0 66-67 33.86152230263866 36.0 33.0 39.5 26.0 41.0 68-69 33.62664084697404 36.0 33.0 39.0 26.0 41.0 70-71 33.45999536482709 35.5 33.0 39.0 26.0 41.0 72-73 33.16401860170306 35.0 33.0 38.5 26.0 40.0 74-75 32.84594235946402 35.0 33.0 37.0 26.0 39.5 76-77 32.5343087533588 35.0 33.0 37.0 25.0 39.0 78-79 32.20857176510266 35.0 33.0 36.0 25.0 39.0 80-81 31.934889590520182 35.0 33.0 36.0 24.0 38.0 82-83 31.6789591960729 35.0 33.0 35.5 24.0 37.0 84-85 31.44789476716942 35.0 32.0 35.0 23.0 37.0 86-87 31.250080077484498 35.0 32.0 35.0 23.0 36.0 88-89 31.08552402451642 35.0 32.0 35.0 21.5 36.0 90-91 30.945351565578378 35.0 32.0 35.0 20.0 36.0 92-93 30.825810074849144 35.0 32.0 35.0 20.0 36.0 94-95 30.695260683988828 35.0 32.0 35.0 19.5 35.0 96-97 30.60649352135018 35.0 32.0 35.0 19.0 35.0 98-99 30.527678634225538 35.0 32.0 35.0 18.5 35.0 100-101 30.429090540095093 35.0 32.0 35.0 18.0 35.0 102-103 29.44379344415379 33.5 29.5 34.5 15.0 35.0 104-105 30.137196881299747 34.5 31.0 35.0 15.0 35.0 106-107 30.245951426438452 35.0 31.5 35.0 15.0 35.0 108-109 30.2166439144925 35.0 31.5 35.0 12.5 35.0 110-111 30.141812776299098 35.0 31.0 35.0 10.0 35.0 112-113 30.3010345672045 35.0 32.0 35.0 8.5 35.0 114-115 30.29322701246582 35.0 32.0 35.0 4.5 35.0 116-117 30.20288075572808 35.0 32.0 35.0 2.0 35.0 118-119 29.96362236644643 35.0 32.0 35.0 2.0 35.0 120-121 29.901095197299902 35.0 32.0 35.0 2.0 35.0 122-123 29.775663986401234 35.0 32.0 35.0 2.0 35.0 124-125 28.890187711792237 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5212489769248556 1101 2 0.5754917793236523 1101 3 0.6398681849314869 1101 4 0.6336919943825308 1101 5 0.4927676308159974 1101 6 0.5811993249797638 1101 7 0.5629835664664711 1101 8 0.5366227678200559 1101 9 0.686700648856494 1101 10-11 0.6259575768161483 1101 12-13 0.6412478911149506 1101 14-15 0.8124230868581037 1101 16-17 0.7282573701885156 1101 18-19 0.7863858521051199 1101 20-21 0.8722769903623089 1101 22-23 0.8516084680957192 1101 24-25 1.0560989324808787 1101 26-27 0.9699787232757515 1101 28-29 0.956840504744747 1101 30-31 0.9445296304157651 1101 32-33 1.0842172603627986 1101 34-35 0.985442622185623 1101 36-37 0.8823839150663773 1101 38-39 0.9216638245780189 1101 40-41 0.809594551615632 1101 42-43 0.8088770810841694 1101 44-45 0.6679566170647533 1101 46-47 0.7145874340783607 1101 48-49 0.7040937050226788 1101 50-51 0.6216792891922864 1101 52-53 0.6378377593181099 1101 54-55 0.6007541061238157 1101 56-57 0.5745890181190987 1101 58-59 0.5067084323995559 1101 60-61 0.38361716829101766 1101 62-63 0.3989229496210456 1101 64-65 0.37327967206742585 1101 66-67 0.40795136925662945 1101 68-69 0.3869975825420937 1101 70-71 0.43700648325254576 1101 72-73 0.41779586198827445 1101 74-75 0.446025540058713 1101 76-77 0.5247627877856615 1101 78-79 0.511187834955237 1101 80-81 0.5158289450877724 1101 82-83 0.6035959429474467 1101 84-85 0.5116042532706793 1101 86-87 0.5010958691481697 1101 88-89 0.5571482661216081 1101 90-91 0.5981307196748347 1101 92-93 0.5517758167129436 1101 94-95 0.48757959657962857 1101 96-97 0.5408063482986663 1101 98-99 0.48028022605962306 1101 100-101 0.4309240061711428 1101 102-103 0.33900539259042617 1101 104-105 0.5460068284332991 1101 106-107 0.4085956332837526 1101 108-109 0.3240980186693889 1101 110-111 0.2876169686710526 1101 112-113 0.35512615550173976 1101 114-115 0.37043565975959325 1101 116-117 0.41741541284578787 1101 118-119 0.2968297596413372 1101 120-121 0.2010015274410435 1101 122-123 0.210311621295439 1101 124-125 0.1434788545148784 1102 1 0.18034508475355082 1102 2 0.11564614411312135 1102 3 0.0942888843630314 1102 4 0.24241905311880885 1102 5 0.2757303098326176 1102 6 0.31122082374879767 1102 7 0.20958717671223326 1102 8 0.27315690235293744 1102 9 0.2898225051250307 1102 10-11 0.2980030290632101 1102 12-13 0.25826464917506087 1102 14-15 0.30770862335376137 1102 16-17 0.2880902599181212 1102 18-19 0.33067463259856567 1102 20-21 0.4867953371931719 1102 22-23 0.41604164975140634 1102 24-25 0.6937362814914749 1102 26-27 0.7353930501273567 1102 28-29 0.49073846769905316 1102 30-31 0.6082970212151579 1102 32-33 0.79628496743954 1102 34-35 0.7382640045437938 1102 36-37 0.541237072197454 1102 38-39 0.5985658428104728 1102 40-41 0.5284809304687883 1102 42-43 0.4897145073381566 1102 44-45 0.4357425825566068 1102 46-47 0.4173828439073688 1102 48-49 0.3903602005082334 1102 50-51 0.344668380569523 1102 52-53 0.32758237455306727 1102 54-55 0.4063776964359036 1102 56-57 0.3355057472691172 1102 58-59 0.3668731300178436 1102 60-61 0.32198369846546626 1102 62-63 0.3038251681854902 1102 64-65 0.36457048750371257 1102 66-67 0.42581992353864706 1102 68-69 0.37000407615595066 1102 70-71 0.3692833141352594 1102 72-73 0.4489181914769418 1102 74-75 0.4645279572857248 1102 76-77 0.33688528919384453 1102 78-79 0.38908809402239086 1102 80-81 0.4081901481648593 1102 82-83 0.28544819200313043 1102 84-85 0.26032039487650493 1102 86-87 0.18478774538088416 1102 88-89 0.21274362053827645 1102 90-91 0.13705259324768804 1102 92-93 0.26649041826657793 1102 94-95 0.2901795462719008 1102 96-97 0.2270274560671659 1102 98-99 0.17256105555606993 1102 100-101 0.21204368703971 1102 102-103 0.11876584978399052 1102 104-105 0.24164679042801396 1102 106-107 0.24668115289244952 1102 108-109 0.2232536546359789 1102 110-111 0.2761441898239312 1102 112-113 0.32406634949951396 1102 114-115 0.34341720658262176 1102 116-117 0.3863562489332857 1102 118-119 0.09770357151450426 1102 120-121 0.18753951451224538 1102 122-123 0.2098725523191085 1102 124-125 0.09149266233324838 1103 1 0.24920575694366676 1103 2 -0.04571009435878892 1103 3 0.3375486987836176 1103 4 0.24590304719926337 1103 5 0.2477284599351819 1103 6 0.2997199385487903 1103 7 0.3437549088759795 1103 8 0.2511215273867222 1103 9 0.4541241618977381 1103 10-11 0.41374920041305074 1103 12-13 0.3409582597583807 1103 14-15 0.5094259376002981 1103 16-17 0.4458940439030741 1103 18-19 0.5957294773339186 1103 20-21 0.6559833458319986 1103 22-23 0.6374637908193677 1103 24-25 0.7919194256595219 1103 26-27 0.7570625439695462 1103 28-29 0.7116543722323669 1103 30-31 0.7356606225381839 1103 32-33 0.9030897620452052 1103 34-35 0.9092824993502973 1103 36-37 0.7553726565274772 1103 38-39 0.8603787747111227 1103 40-41 0.7804398474328735 1103 42-43 0.7398363390762199 1103 44-45 0.6790827819978986 1103 46-47 0.5408825229075589 1103 48-49 0.4459669920275431 1103 50-51 0.3570697478017948 1103 52-53 0.38658038742609335 1103 54-55 0.38777680331703124 1103 56-57 0.4324473422839077 1103 58-59 0.49577902003631635 1103 60-61 0.49270959642750256 1103 62-63 0.43795542850329383 1103 64-65 0.4570535888975158 1103 66-67 0.4961348583885794 1103 68-69 0.4212703852848634 1103 70-71 0.38640864237088124 1103 72-73 0.37441698381066857 1103 74-75 0.39282193321760417 1103 76-77 0.40452876314191855 1103 78-79 0.38281871845384785 1103 80-81 0.33233655803784856 1103 82-83 0.28371182924663785 1103 84-85 0.26406468514739245 1103 86-87 0.3003240975480068 1103 88-89 0.2761044599817204 1103 90-91 0.28546171078455984 1103 92-93 0.2835491384629556 1103 94-95 0.21728086782788125 1103 96-97 0.23635983666627425 1103 98-99 0.23828891162466448 1103 100-101 0.2358456415032748 1103 102-103 0.19431159962766031 1103 104-105 0.2446619426378014 1103 106-107 0.25415885077659794 1103 108-109 0.25595496026160447 1103 110-111 0.26544553400966464 1103 112-113 0.33089834303104837 1103 114-115 0.30481915993476605 1103 116-117 0.3589444551888228 1103 118-119 0.27862918245223867 1103 120-121 0.2290850633740682 1103 122-123 0.2546079547775406 1103 124-125 0.23269541519797698 1104 1 0.19429006504714508 1104 2 0.20164412378575136 1104 3 0.1828078070273662 1104 4 0.17929714169068944 1104 5 0.17125222055890532 1104 6 0.2146617446454684 1104 7 0.21014800657454202 1104 8 0.21549470791628522 1104 9 0.23921407012267082 1104 10-11 0.24517432350670276 1104 12-13 0.265924458968243 1104 14-15 0.21795502960034696 1104 16-17 0.28022157449788665 1104 18-19 0.43734946500757843 1104 20-21 0.4017554817456386 1104 22-23 0.432910848946662 1104 24-25 0.6688547599900474 1104 26-27 0.6617570542292626 1104 28-29 0.4814179012736588 1104 30-31 0.529259488841042 1104 32-33 0.7088329932817388 1104 34-35 0.7372342213836447 1104 36-37 0.5946544417490784 1104 38-39 0.5950424323906844 1104 40-41 0.5017554574839309 1104 42-43 0.4642204355299313 1104 44-45 0.3616925040866832 1104 46-47 0.3587864514342911 1104 48-49 0.32811562254148186 1104 50-51 0.34561833001348674 1104 52-53 0.324486604878679 1104 54-55 0.2709685083647315 1104 56-57 0.23996245450556586 1104 58-59 0.32137544854527533 1104 60-61 0.2592274589541077 1104 62-63 0.26848774272952625 1104 64-65 0.2810926134061944 1104 66-67 0.3330110352466775 1104 68-69 0.23274435689947381 1104 70-71 0.22757555689803155 1104 72-73 0.20633375476082705 1104 74-75 0.2028436828051241 1104 76-77 0.14682166786310802 1104 78-79 0.19528377260179752 1104 80-81 0.2547980765700437 1104 82-83 0.20186707640151624 1104 84-85 0.2060057533729598 1104 86-87 0.24768319543328943 1104 88-89 0.2400623034941063 1104 90-91 0.2532340979529337 1104 92-93 0.15293604895373392 1104 94-95 0.23883211105564328 1104 96-97 0.30572882549054725 1104 98-99 0.24921689535651836 1104 100-101 0.2813910731275229 1104 102-103 0.18362109394994874 1104 104-105 0.24226763514688798 1104 106-107 0.2820198942887906 1104 108-109 0.2682015521700052 1104 110-111 0.17468191687747847 1104 112-113 0.20855565537047838 1104 114-115 0.17637911242158566 1104 116-117 0.15213465318730002 1104 118-119 0.1534358396964528 1104 120-121 0.19303563918746747 1104 122-123 0.22262764337341068 1104 124-125 0.25473095871843654 1105 1 0.40823467744047903 1105 2 0.32882123430098176 1105 3 0.41395371096844613 1105 4 0.4103443859525271 1105 5 0.44799795378266083 1105 6 0.26063331230620435 1105 7 0.2025611713519666 1105 8 0.25775618539753253 1105 9 0.23224256386935593 1105 10-11 0.3472698770529874 1105 12-13 0.3593356863926829 1105 14-15 0.36923153135084874 1105 16-17 0.4144439115355141 1105 18-19 0.5822422620118388 1105 20-21 0.6349948844765692 1105 22-23 0.5997008402123782 1105 24-25 0.8149729211900834 1105 26-27 0.7437656774616741 1105 28-29 0.6358542184505609 1105 30-31 0.717948164458754 1105 32-33 0.9097281769404972 1105 34-35 0.7922981254851891 1105 36-37 0.694527407608831 1105 38-39 0.7417977249374132 1105 40-41 0.66500456227282 1105 42-43 0.5918175947271962 1105 44-45 0.5143133419991059 1105 46-47 0.5542144536706815 1105 48-49 0.5378854325698299 1105 50-51 0.48635165690021864 1105 52-53 0.4521705814566559 1105 54-55 0.44800216629467826 1105 56-57 0.5260526239625776 1105 58-59 0.5152409339936384 1105 60-61 0.4888324916329694 1105 62-63 0.473660858704136 1105 64-65 0.4714574840353478 1105 66-67 0.46680861805316454 1105 68-69 0.563604963399257 1105 70-71 0.5680227001317206 1105 72-73 0.603891245395026 1105 74-75 0.46447448652153867 1105 76-77 0.49901315579144523 1105 78-79 0.4349580137124107 1105 80-81 0.32091562918573047 1105 82-83 0.46958619402009205 1105 84-85 0.4641663714876145 1105 86-87 0.41194364036021014 1105 88-89 0.421400715369721 1105 90-91 0.4439058095956341 1105 92-93 0.43523334978019435 1105 94-95 0.4547009401103388 1105 96-97 0.4765124162683918 1105 98-99 0.41545982732769104 1105 100-101 0.3995919566257342 1105 102-103 0.420457261553139 1105 104-105 0.32094923910858597 1105 106-107 0.4392154670258783 1105 108-109 0.3541724438001914 1105 110-111 0.3578786677272028 1105 112-113 0.41497240875649055 1105 114-115 0.4325281867263584 1105 116-117 0.49913886143827213 1105 118-119 0.38734841043752155 1105 120-121 0.5191365827833856 1105 122-123 0.5310733275731891 1105 124-125 0.47113861235677135 1106 1 0.19678284742072094 1106 2 0.27781797502110805 1106 3 0.32296330715473687 1106 4 0.2688406814213238 1106 5 0.2714998954441441 1106 6 0.20733716290332893 1106 7 0.2787570860994748 1106 8 0.19423222533123408 1106 9 0.24193210391555198 1106 10-11 0.24444640164579567 1106 12-13 0.19178639564709954 1106 14-15 0.3465608023093054 1106 16-17 0.323100606742706 1106 18-19 0.36244366976566056 1106 20-21 0.3968733136569256 1106 22-23 0.35936665160701864 1106 24-25 0.5889881932995564 1106 26-27 0.6679491609971819 1106 28-29 0.5285250121559315 1106 30-31 0.6250131632743674 1106 32-33 0.6892032315312591 1106 34-35 0.7250921922297024 1106 36-37 0.6093043482501841 1106 38-39 0.6280175586116457 1106 40-41 0.5446784841465728 1106 42-43 0.467560178882529 1106 44-45 0.4375708692588134 1106 46-47 0.36599637394702 1106 48-49 0.2993854297082734 1106 50-51 0.3839470098454214 1106 52-53 0.32797303539687306 1106 54-55 0.268829441110519 1106 56-57 0.3089812100493461 1106 58-59 0.28767963510100714 1106 60-61 0.21102944293419768 1106 62-63 0.29447995763377577 1106 64-65 0.2938391063080701 1106 66-67 0.3024319427862068 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120-121 -1.9452118750222844 2216 122-123 -2.051237112104431 2216 124-125 -1.9267999390756323 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45087.0 3 8651.0 4 5854.0 5 5131.0 6 5581.0 7 6431.0 8 6493.0 9 7145.0 10 8211.0 11 9429.0 12 9971.0 13 9654.0 14 9510.0 15 9367.0 16 8597.0 17 7933.0 18 7270.0 19 7003.0 20 6781.0 21 7043.0 22 8278.0 23 11075.0 24 16918.0 25 24286.0 26 27966.0 27 27143.0 28 31105.0 29 37356.0 30 43685.0 31 54256.0 32 69233.0 33 91895.0 34 132031.0 35 209291.0 36 415981.0 37 698425.0 38 269409.0 39 739.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.284733869094154 17.98480051416759 12.520031677607424 24.210433939130834 2 15.998599447493906 20.12259921180771 39.12662156130236 24.752179779396023 3 19.07712497313675 25.096315612560517 28.62687783123818 27.19968158306456 4 12.607896927597368 16.366338362730353 36.43792207175665 34.58784263791563 5 13.723129822050902 38.7904191819818 33.583727461773286 13.902723534194013 6 32.73216155328156 36.358894680042866 17.177440232734618 13.731503533940959 7 28.975406797290916 32.81448009812707 20.564400126260217 17.645712978321797 8 26.58199637829451 35.34709140781302 19.481623276533398 18.58928893735907 9 26.69274921309601 16.624615127792193 18.88667014942384 37.79596550968796 10-11 24.964657554851495 26.963558035652813 27.282757568727895 20.7890268407678 12-13 25.45336987889375 24.473901970163876 27.151984251908452 22.920743899033923 14-15 22.677671690791534 25.969094238040213 24.46249236762679 26.890741703541465 16-17 23.03208220019105 28.845909809182967 25.275507962343767 22.846500028282215 18-19 23.216370230105728 27.551079641488098 26.699035502474693 22.53351462593148 20-21 23.986032046546285 26.84201750570504 26.421928042836072 22.7500224049126 22-23 23.66058517036446 26.739436695816632 26.36288071680393 23.237097417014983 24-25 23.270543113230012 26.917348760705963 26.67768016561772 23.134427960446313 26-27 23.08891717737076 27.115738782802374 26.33446958107715 23.460874458749714 28-29 23.19692172948058 27.301240049635034 26.24205702774948 23.259781193134906 30-31 23.261307565353903 26.953922293525306 26.226221221295447 23.55854891982534 32-33 22.859243205682684 27.155300346058148 26.248430980625194 23.737025467633977 34-35 23.59853104576964 27.14302301830599 26.1178400982467 23.140605837677676 36-37 23.79855039545456 26.725664204327792 26.065676306966306 23.41010909325134 38-39 23.304068337353808 26.854094867409056 26.18304551472545 23.658791280511682 40-41 23.50369714080151 26.78173967095002 26.385649220975505 23.328913967272964 42-43 23.264467720952226 26.866804241123493 26.07601592508018 23.792712112844104 44-45 23.315461324709236 26.997508906522455 26.28367366696629 23.40335610180202 46-47 23.657853654754295 27.14080855770496 25.86663943693779 23.334698350602956 48-49 23.3269841511957 27.122744287272 25.89387989262531 23.65639166890699 50-51 23.301587086375836 27.178949518128746 26.03729640272285 23.482166992772566 52-53 23.65118610882962 26.843406348383837 26.086301633077706 23.419105909708833 54-55 23.155476659741037 27.01342690665062 26.164767256287885 23.66632917732046 56-57 23.14112038994107 27.32555516033611 25.900519521734093 23.632804927988733 58-59 23.249289571849058 26.817839082330003 26.024858682371615 23.90801266344932 60-61 23.023079460748264 26.98176199892746 26.30554674487584 23.689611795448435 62-63 23.107065955143714 26.97260854153826 26.492379278995067 23.427946224322955 64-65 22.923158990559532 26.998604755018622 26.216941316241833 23.861294938180013 66-67 22.954792793506606 27.189490032610607 26.01707157873016 23.83864559515262 68-69 23.35003269107968 26.802617489791185 26.3004079287415 23.546941890387636 70-71 23.187502211183432 26.654913233691847 26.254474534899412 23.90311002022531 72-73 23.128121245581028 26.952075421093408 26.03661950765115 23.883183825674415 74-75 23.01146390270957 27.254107824324386 26.11686935506491 23.617558917901135 76-77 23.241621764188107 26.851262018275936 26.222228342211434 23.68488787532452 78-79 23.298908093741108 27.039726694005456 26.136834766264776 23.524530445988656 80-81 23.386988115162172 27.094591445458978 25.980912230154864 23.53750820922399 82-83 23.258428061237677 27.145375714828653 26.14481685938494 23.451379364548735 84-85 23.095306902650666 27.20673544260136 26.16971491575612 23.528242738991853 86-87 23.24328916503959 27.008631969302527 26.116368030697473 23.63171083496041 88-89 23.18032975118143 27.042821216321595 26.07155787487657 23.705291157620405 90-91 23.07912791803714 27.10045500554828 26.17273347792615 23.647683598488427 92-93 23.20143888702343 26.851830862982663 26.255493542274195 23.691236707719717 94-95 23.01454444385128 26.625890703131155 26.563481108069016 23.79608374494855 96-97 23.1939353598232 26.417343135439648 26.462954198696455 23.9257673060407 98-99 22.81057928125203 26.73731613120865 26.270844890578232 24.181259696961092 100-101 22.804424838761623 26.67545956116041 26.54451959014518 23.975596009932794 102-103 22.773745978646705 26.395818745941575 26.64348220329378 24.186953072117948 104-105 22.570814132104456 26.801186503522434 26.321161078446952 24.306838285926162 106-107 22.83042126349593 26.804209350311503 26.147406940100527 24.21796244609204 108-109 22.770169871845116 26.756203205588026 26.128775171161994 24.344851751404864 110-111 22.640578499230564 26.916785019422278 26.29805997518829 24.144576506158863 112-113 22.863889704036197 27.029185334128584 26.313583697147997 23.793341264687218 114-115 22.610147531607332 27.284711907658554 26.15530118007909 23.94983938065503 116-117 22.647234119757595 27.161119476124107 26.281754550171428 23.909891853946874 118-119 22.481416504168266 27.23212425451289 26.12340750857422 24.163051732744627 120-121 22.44671236970382 27.263686361475912 26.280533055520856 24.009068213299408 122-123 22.570270879017148 27.261373958652708 26.175133017635176 23.993222144694965 124-125 22.587781749556893 27.344041552082494 26.074909359844607 23.99326733851601 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1648.0 1 1283.5 2 790.0 3 577.5 4 443.5 5 383.5 6 346.0 7 335.0 8 350.5 9 347.5 10 331.5 11 321.0 12 343.0 13 382.5 14 434.0 15 501.5 16 559.0 17 642.5 18 785.5 19 979.0 20 1262.0 21 1707.0 22 2348.0 23 3238.0 24 4548.5 25 6328.5 26 8924.0 27 12300.5 28 15449.5 29 19255.5 30 23790.0 31 29912.0 32 38051.0 33 42358.0 34 44347.0 35 47867.0 36 52234.0 37 56349.5 38 59159.5 39 61145.0 40 63026.0 41 66101.5 42 68073.0 43 70235.5 44 73298.5 45 76795.0 46 78779.5 47 79802.5 48 83319.5 49 85839.0 50 85699.5 51 85376.5 52 86195.5 53 88775.5 54 92422.5 55 90232.0 56 82545.5 57 75219.5 58 70699.0 59 63840.5 60 52627.0 61 43942.5 62 38016.5 63 29836.0 64 21123.5 65 15657.0 66 11606.0 67 9076.5 68 7269.5 69 5611.5 70 4482.0 71 3317.5 72 2457.5 73 2062.5 74 1667.5 75 1264.0 76 883.5 77 525.0 78 337.0 79 192.5 80 112.0 81 83.5 82 57.5 83 35.5 84 33.0 85 25.5 86 18.5 87 14.0 88 10.0 89 9.5 90 9.5 91 7.0 92 6.0 93 6.0 94 6.0 95 4.5 96 2.0 97 1.5 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06143510715553759 2 0.04851255013316589 3 0.044190908112569455 4 0.043131682127129145 5 0.04236903941761213 6 0.0042792729811788255 7 3.813213547585092E-4 8 0.0 9 6.35535591264182E-4 10-11 0.00387676710671151 12-13 0.002330297167968667 14-15 0.008516176922940039 16-17 0.003114124397194492 18-19 0.005296129927201516 20-21 0.010189753979935719 22-23 0.017180645483841718 24-25 0.0033895231534089706 26-27 0.016947615767044854 28-29 0.007944194890802274 30-31 0.007584058055752571 32-33 0.002605695924183146 34-35 0.018642377343749336 36-37 0.01686287768820963 38-39 0.026523018675425197 40-41 0.006228248794388984 42-43 0.001588838978160455 44-45 2.3302971679686673E-4 46-47 0.0020548984117541884 48-49 0.002859910160688819 50-51 0.0016947615767044853 52-53 0.001631208017578067 54-55 0.005910480998756892 56-57 0.002648064963600758 58-59 0.008198409127307947 60-61 0.01646037181374231 62-63 0.012689527305574834 64-65 0.002330297167968667 66-67 0.003919136146129122 68-69 0.010295676578479749 70-71 0.002224374569424637 72-73 0.0033259695942825527 74-75 9.74487906605079E-4 76-77 2.753987562144789E-4 78-79 0.003198862476029716 80-81 0.0027116185227271765 82-83 0.0013981783007812004 84-85 0.011863331036931397 86-87 0.00635535591264182 88-89 0.0017159460964132913 90-91 9.74487906605079E-4 92-93 0.0023938507270950854 94-95 0.0 96-97 0.0017371306161220975 98-99 0.024701149980467872 100-101 0.014998639953834695 102-103 0.006630754668856299 104-105 0.014363104362570512 106-107 0.031882702161753124 108-109 9.74487906605079E-4 110-111 0.001610023497869261 112-113 0.016163788537819027 114-115 0.03192507120117074 116-117 0.05008020459161754 118-119 0.35573045495027145 120-121 0.11645130483930695 122-123 0.019934633045986506 124-125 0.31793727178976144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2360214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.73551735198117 #Duplication Level Percentage of deduplicated Percentage of total 1 84.27232766375504 58.767743680876315 2 9.36363935562187 13.059564695233256 3 2.8638345420862783 5.991329502085821 4 1.2640726902411314 3.5260305209790377 5 0.6588697045582643 2.297330985745877 6 0.3635546834491053 1.521160436163546 7 0.23812402602952681 1.1623991504373976 8 0.17713397273818127 0.988202338360704 9 0.1273813137303467 0.7994701632564672 >10 0.6088426915147466 7.79198419151329 >50 0.045076885928629656 2.139009197155808 >100 0.01700641921308001 1.9033087312174106 >500 1.3605113374523317E-4 0.05246640697520222 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.236903941761213E-5 0.0 14-15 0.0 0.0 0.0 4.236903941761213E-5 8.473807883522427E-5 16-17 0.0 0.0 0.0 4.236903941761213E-5 8.473807883522427E-5 18-19 0.0 0.0 0.0 1.4829163796164246E-4 1.6947615767044853E-4 20-21 0.0 0.0 0.0 2.753987562144789E-4 1.6947615767044853E-4 22-23 0.0 0.0 0.0 4.448749138849274E-4 1.906606773792546E-4 24-25 0.0 0.0 0.0 6.56720110972988E-4 2.1184519708806066E-4 26-27 0.0 0.0 0.0 0.001588838978160455 2.753987562144789E-4 28-29 0.0 0.0 0.0 0.0027751720818535944 2.9658327592328493E-4 30-31 0.0 0.0 0.0 0.008473807883522427 3.1776779563209097E-4 32-33 0.0 0.0 0.0 0.02256151348987846 4.236903941761213E-4 34-35 2.1184519708806066E-5 0.0 0.0 0.04397906291548139 4.448749138849274E-4 36-37 4.236903941761213E-5 0.0 0.0 0.07624308643199303 4.6605943359373346E-4 38-39 4.236903941761213E-5 0.0 0.0 0.1257724935111816 4.6605943359373346E-4 40-41 4.236903941761213E-5 0.0 0.0 0.1815301493847592 4.6605943359373346E-4 42-43 4.236903941761213E-5 0.0 0.0 0.2361014721546436 4.6605943359373346E-4 44-45 4.236903941761213E-5 0.0 0.0 0.3050358992870985 4.6605943359373346E-4 46-47 4.236903941761213E-5 0.0 0.0 0.3899858233194109 4.872439533025395E-4 48-49 4.236903941761213E-5 0.0 0.0 0.4797870023650398 5.507975124289577E-4 50-51 4.236903941761213E-5 0.0 0.0 0.5818752028417762 5.507975124289577E-4 52-53 4.236903941761213E-5 0.0 0.0 0.6805103266059772 6.355355912641819E-4 54-55 4.236903941761213E-5 0.0 0.0 0.7877887344113712 7.202736700994062E-4 56-57 6.35535591264182E-5 0.0 0.0 0.9130527994495414 7.414581898082123E-4 58-59 8.473807883522427E-5 0.0 0.0 1.0590141402432152 7.626427095170184E-4 60-61 8.473807883522427E-5 0.0 0.0 1.2220290194024779 7.626427095170184E-4 62-63 8.473807883522427E-5 0.0 0.0 1.385848910310675 8.050117489346305E-4 64-65 8.473807883522427E-5 0.0 0.0 1.5487154978319762 8.050117489346305E-4 66-67 8.473807883522427E-5 0.0 0.0 1.7465789119122248 8.050117489346305E-4 68-69 8.473807883522427E-5 0.0 0.0 1.9714102195817835 8.050117489346305E-4 70-71 8.473807883522427E-5 0.0 0.0 2.2255185334889127 8.473807883522427E-4 72-73 8.473807883522427E-5 0.0 0.0 2.4957482668944424 8.473807883522427E-4 74-75 8.473807883522427E-5 0.0 0.0 2.7566356271083894 8.473807883522427E-4 76-77 8.473807883522427E-5 0.0 0.0 3.048346463498649 8.473807883522427E-4 78-79 8.473807883522427E-5 0.0 0.0 3.385752308900803 8.473807883522427E-4 80-81 8.473807883522427E-5 0.0 0.0 3.7558034991742275 9.109343474786608E-4 82-83 8.473807883522427E-5 0.0 0.0 4.147484084070342 0.0011015950248579153 84-85 8.473807883522427E-5 0.0 0.0 4.542638930198702 0.0011015950248579153 86-87 8.473807883522427E-5 0.0 0.0 4.941310406598724 0.0011015950248579153 88-89 8.473807883522427E-5 0.0 0.0 5.374470281084681 0.0011015950248579153 90-91 8.473807883522427E-5 0.0 0.0 5.871416744413853 0.0011015950248579153 92-93 8.473807883522427E-5 0.0 0.0 6.38622175785755 0.0011439640642755275 94-95 8.473807883522427E-5 0.0 0.0 6.905433151400678 0.0011439640642755275 96-97 8.473807883522427E-5 0.0 0.0 7.4279281454986705 0.0011439640642755275 98-99 8.473807883522427E-5 0.0 0.0 7.979488300637145 0.0011439640642755275 100-101 8.473807883522427E-5 0.0 0.0 8.59900839500147 0.0011863331036931397 102-103 8.473807883522427E-5 0.0 0.0 9.262126230926517 0.001228702143110752 104-105 8.473807883522427E-5 0.0 0.0 9.925222882331855 0.001228702143110752 106-107 8.473807883522427E-5 0.0 0.0 10.576858708574731 0.001228702143110752 108-109 8.473807883522427E-5 0.0 0.0 11.242730532061923 0.00129225570223717 110-111 8.473807883522427E-5 0.0 0.0 11.973723569133986 0.0013558092613635882 112-113 8.473807883522427E-5 0.0 0.0 12.751110704368333 0.0013558092613635882 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2470 0.0 53.72899 1 GTATCAA 4415 0.0 50.952072 1 ATCAACG 5035 0.0 43.135906 3 CAACGCA 5130 0.0 42.220203 5 TCAACGC 5255 0.0 41.443256 4 AACGCAG 5495 0.0 39.176773 6 TATCAAC 5920 0.0 37.592793 2 GTACATG 11850 0.0 36.86196 1 TACATGG 11750 0.0 36.310818 2 ACATGGG 11940 0.0 33.888325 3 ACGCAGA 6695 0.0 31.619835 7 CGCAGAG 6750 0.0 31.097906 8 CATGGGG 6115 0.0 29.678976 4 CATGGGA 6255 0.0 26.351051 4 TAAGGTA 845 0.0 24.646591 4 GCAGAGT 8620 0.0 23.3883 9 GGTAAGG 1175 0.0 22.788733 3 ATGGGGG 3250 0.0 22.153294 5 GTGTAAG 1165 0.0 21.96561 1 AGGTAAG 1145 0.0 21.827225 2 >>END_MODULE