FastQCFastQC Report
Thu 26 May 2016
SRR1364190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360214
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76970.32611449639736056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65020.2754834942933141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.1603668141956619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45350.070.6420061
GTATCAA87700.056.695021
ATCAACG113450.043.804533
CAACGCA116100.042.7938045
TATCAAC116650.042.605572
TCAACGC117150.042.4210324
AACGCAG121650.041.0344736
ACGCAGA140700.035.2634627
CGCAGAG143600.034.3441778
GTGGTAT19650.032.727981
GTACATG115100.032.6963841
TACATGG115300.032.1605452
ACATGGG114600.031.0046463
GCAGAGT164200.029.6731599
TGGTATC21050.029.4066772
CATGGGG58250.026.7696344
AGAGTAC147600.026.60177410-11
GAGTACT100950.026.51912512-13
AGTACTT104000.025.68419812-13
CAGAGTA155150.025.49898510-11
Produced by FastQC (version 0.11.5)