FastQCFastQC Report
Thu 26 May 2016
SRR1364189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758070
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29010.16501049446267782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.051.497961
GTATCAA37000.045.022261
GTACATG103450.037.8988341
CAACGCA42500.037.6506545
ATCAACG42750.037.429413
TCAACGC43100.037.2624134
AACGCAG44650.035.959666
TACATGG106550.035.6754072
ACATGGG105250.034.305473
TATCAAC50350.033.0811122
CATGGGG38450.030.6304444
CATGGGA69550.030.2754484
ACGCAGA55900.028.6155037
CGCAGAG56450.027.9145418
GAGTACA73550.027.178631
AGTACAT72300.026.5757642
ATGGGAG31350.025.9951425
GCAGAGT63500.023.2260829
CATATAG5450.022.9202633
ATGGGGA22150.022.827295
Produced by FastQC (version 0.11.5)