FastQCFastQC Report
Thu 26 May 2016
SRR1364189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758070
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76870.4372408379643586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.39389785389660253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39820.22649837606011136No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20170.11472808249955917No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.076.0956651
GTATCAA79900.054.3103181
ATCAACG102550.042.005563
TCAACGC104150.041.3602524
CAACGCA106350.040.4429635
AACGCAG108650.039.804716
TATCAAC108300.039.721542
TGGTATC17600.036.1732372
GTACATG102750.035.4544831
TACATGG104500.034.3903772
ACGCAGA126950.034.1136677
CGCAGAG128150.033.7478078
ACATGGG103650.033.580753
GTGGTAT20250.032.9226341
GCAGAGT140200.030.6775059
CATGGGG39150.029.4831494
CATGGGA66050.028.2852944
CGCGTCA557.76224E-427.04079426-27
CAGAGTA137300.027.03608710-11
GAGTACT90050.026.98674212-13
Produced by FastQC (version 0.11.5)