FastQCFastQC Report
Thu 26 May 2016
SRR1364188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889462
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21000.11114274857075718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.053.3625141
GTATCAA35700.040.8327871
GTACATG94300.036.6585771
TACATGG93800.035.644062
ATCAACG40850.034.076463
TCAACGC41450.033.151764
ACATGGG97100.032.899233
CAACGCA42450.032.2323765
AACGCAG44000.031.2213766
TATCAAC47350.029.9027162
CATGGGG44250.027.5587674
ACGCAGA52100.026.2532377
CATGGGA56650.026.2518144
CGCAGAG52700.025.6158038
AGTACAT64650.023.833342
GAGTACA67200.023.728851
GTATAGG6100.022.4341281
TATACGA1350.00255844322.0332435
ATGGGAG27800.021.6131865
ATGGGGA26350.021.447925
Produced by FastQC (version 0.11.5)