##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1889462 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.329495909417602 31.0 31.0 34.0 26.0 34.0 2 30.354486621059326 31.0 31.0 34.0 26.0 34.0 3 30.402206024783773 31.0 31.0 34.0 26.0 34.0 4 33.74121469497666 37.0 35.0 37.0 28.0 37.0 5 33.864043838934045 37.0 35.0 37.0 28.0 37.0 6 33.85416271933492 37.0 35.0 37.0 29.0 37.0 7 33.778830693604846 37.0 35.0 37.0 28.0 37.0 8 33.72872330853968 37.0 35.0 37.0 28.0 37.0 9 35.09354779296964 39.0 35.0 39.0 27.0 39.0 10-11 35.076267741822804 39.0 35.0 39.0 27.0 39.0 12-13 35.02132432406685 39.0 35.0 39.0 27.0 39.0 14-15 36.233130647771695 40.0 36.0 41.0 27.0 41.0 16-17 36.262079629016085 40.0 36.0 41.0 27.0 41.0 18-19 36.14359828353256 40.0 36.0 41.0 27.0 41.0 20-21 36.10715642865535 40.0 36.0 41.0 27.0 41.0 22-23 36.14385893974052 40.0 36.0 41.0 27.0 41.0 24-25 36.01055538560712 40.0 36.0 41.0 26.5 41.0 26-27 36.01587330150063 40.0 36.0 41.0 27.0 41.0 28-29 36.10891936434816 40.0 36.0 41.0 27.0 41.0 30-31 35.99663343322067 40.0 36.0 41.0 27.0 41.0 32-33 35.80307410257523 40.0 35.5 41.0 26.5 41.0 34-35 35.757331716647386 40.0 35.0 41.0 26.5 41.0 36-37 35.77621910363902 40.0 35.0 41.0 27.0 41.0 38-39 35.60323891139383 40.0 35.0 41.0 26.0 41.0 40-41 35.59557720663342 40.0 35.0 41.0 26.0 41.0 42-43 35.50922855288965 40.0 35.0 41.0 26.0 41.0 44-45 35.48856711593035 40.0 35.0 41.0 26.0 41.0 46-47 35.406546413741054 40.0 35.0 41.0 26.0 41.0 48-49 35.30411910903739 39.0 35.0 41.0 26.0 41.0 50-51 35.17330779872789 39.0 35.0 41.0 26.0 41.0 52-53 35.020836619101104 39.0 34.0 41.0 26.0 41.0 54-55 34.82785734775296 39.0 34.0 41.0 26.0 41.0 56-57 34.6302730618557 38.5 34.0 40.5 25.0 41.0 58-59 34.39595794993495 38.0 34.0 40.0 24.5 41.0 60-61 34.14209997343159 38.0 33.0 40.0 24.0 41.0 62-63 33.85180702231641 37.0 33.0 40.0 23.0 41.0 64-65 33.5436341138377 37.0 33.0 40.0 23.0 41.0 66-67 33.23758138560077 36.0 33.0 39.5 22.0 41.0 68-69 33.013998164556895 36.0 32.5 39.0 22.0 41.0 70-71 32.85811701955372 35.5 33.0 39.0 22.0 41.0 72-73 32.5545822038231 35.0 32.5 38.5 21.5 40.0 74-75 32.23307031313675 35.0 32.0 37.0 20.5 39.5 76-77 31.91478500229166 35.0 32.0 37.0 20.0 39.0 78-79 31.58229326654889 35.0 32.0 36.5 19.5 39.0 80-81 31.298374087438646 35.0 32.0 36.0 18.0 38.0 82-83 31.033370345632775 35.0 32.0 36.0 17.5 37.0 84-85 30.791545688666933 35.0 32.0 35.0 15.5 37.0 86-87 30.584971277538262 35.0 31.0 35.0 11.5 36.0 88-89 30.413471665479378 35.0 31.0 35.0 9.0 36.0 90-91 30.26407199509702 35.0 31.0 35.0 7.0 36.0 92-93 30.13623534106534 35.0 31.0 35.0 7.0 36.0 94-95 30.00394980158373 35.0 31.0 35.0 3.5 35.0 96-97 29.907251905568884 35.0 31.0 35.0 2.0 35.0 98-99 29.830945793035266 35.0 31.0 35.0 2.0 35.0 100-101 29.724822198064846 35.0 31.0 35.0 2.0 35.0 102-103 28.73828661280301 33.5 29.0 34.5 2.0 35.0 104-105 29.434743593679045 34.0 30.0 35.0 2.0 35.0 106-107 29.550924813518346 34.0 31.0 35.0 2.0 35.0 108-109 29.521118709981998 34.0 31.0 35.0 2.0 35.0 110-111 29.4526322836871 34.0 30.5 35.0 2.0 35.0 112-113 29.61090379166133 35.0 31.0 35.0 2.0 35.0 114-115 29.60124257592902 35.0 31.0 35.0 2.0 35.0 116-117 29.511127770762258 35.0 31.0 35.0 2.0 35.0 118-119 29.277919058440975 35.0 31.0 35.0 2.0 35.0 120-121 29.212752889446836 35.0 31.0 35.0 2.0 35.0 122-123 29.075452694999953 35.0 31.0 35.0 2.0 35.0 124-125 28.192038262743573 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.828127770588015 1101 2 0.8594139566295027 1101 3 0.9053073614931328 1101 4 0.9114839805110293 1101 5 0.7250578922503621 1101 6 0.7249521451989835 1101 7 0.7462090862363766 1101 8 0.640789604881121 1101 9 0.7322753029638989 1101 10-11 0.7291767049081059 1101 12-13 0.8168415854587536 1101 14-15 1.0392611322590284 1101 16-17 1.0261314601134046 1101 18-19 1.0946686511985675 1101 20-21 1.2674473288310253 1101 22-23 1.230302844350355 1101 24-25 1.3660976952183717 1101 26-27 1.3798367821917452 1101 28-29 1.2735995244581204 1101 30-31 1.380281857667839 1101 32-33 1.532089705792913 1101 34-35 1.3963076339867726 1101 36-37 1.3410997155933018 1101 38-39 1.4648618166396474 1101 40-41 1.3140861917897126 1101 42-43 1.3094945453242488 1101 44-45 1.1271015108463018 1101 46-47 1.010623849640588 1101 48-49 0.9867392266832056 1101 50-51 0.9611232735082496 1101 52-53 0.8044158599465732 1101 54-55 0.8815205297218682 1101 56-57 0.814361133236261 1101 58-59 0.869642604483019 1101 60-61 0.7889755237914002 1101 62-63 0.8623927365141881 1101 64-65 0.8353149405792664 1101 66-67 0.726617379562498 1101 68-69 0.7516633762269578 1101 70-71 0.7065361783380908 1101 72-73 0.7117007656001562 1101 74-75 0.7621882321681142 1101 76-77 0.7124894521330809 1101 78-79 0.6439383880485536 1101 80-81 0.7487185337203002 1101 82-83 0.6413334650978193 1101 84-85 0.6665838902940031 1101 86-87 0.6865304357742019 1101 88-89 0.7182192061831429 1101 90-91 0.651204799699336 1101 92-93 0.6554781204253466 1101 94-95 0.6547440913344467 1101 96-97 0.6222827416154253 1101 98-99 0.681699608288298 1101 100-101 0.6445470590849638 1101 102-103 0.5674016889546003 1101 104-105 0.71525112328926 1101 106-107 0.7392482958508246 1101 108-109 0.6510241441806919 1101 110-111 0.6034508083846113 1101 112-113 0.7338385499182714 1101 114-115 0.6623407101548864 1101 116-117 0.6693711187052784 1101 118-119 0.5619810051957437 1101 120-121 0.5241509222379257 1101 122-123 0.584125332623799 1101 124-125 0.5896211877248092 1102 1 0.20646214005103047 1102 2 0.24279350099368102 1102 3 0.19756874365129917 1102 4 0.26099797037990413 1102 5 0.28870970395929874 1102 6 0.3385052509932933 1102 7 0.31329918927391276 1102 8 0.31712316732797063 1102 9 0.3611185586069183 1102 10-11 0.2671069943854434 1102 12-13 0.4316641266277017 1102 14-15 0.5254657362756845 1102 16-17 0.4799467296642206 1102 18-19 0.6092007671589599 1102 20-21 0.692794229285262 1102 22-23 0.663455269794035 1102 24-25 0.8510157862544503 1102 26-27 0.8152836965198844 1102 28-29 0.8067874024821862 1102 30-31 0.9821680461474145 1102 32-33 1.0054474490706937 1102 34-35 0.9641230719307288 1102 36-37 0.7662348007801114 1102 38-39 0.8541724532610218 1102 40-41 0.7063635660005758 1102 42-43 0.6770201140747147 1102 44-45 0.658506301472201 1102 46-47 0.6503293893511923 1102 48-49 0.5656851597771109 1102 50-51 0.4964436017371838 1102 52-53 0.4572189967188649 1102 54-55 0.6540339773436656 1102 56-57 0.6700509943807589 1102 58-59 0.6711611081352444 1102 60-61 0.6423749999722403 1102 62-63 0.6409733677025002 1102 64-65 0.5669321554414921 1102 66-67 0.5128009873907615 1102 68-69 0.5086036358489281 1102 70-71 0.5548375258009202 1102 72-73 0.48843171996561807 1102 74-75 0.4870844162101591 1102 76-77 0.5379663806846118 1102 78-79 0.5984326579160353 1102 80-81 0.6039512710407706 1102 82-83 0.5208612812082727 1102 84-85 0.5265451386908246 1102 86-87 0.5408274791507885 1102 88-89 0.4663471563726631 1102 90-91 0.48856363806204683 1102 92-93 0.5236687891155576 1102 94-95 0.561071172234108 1102 96-97 0.5127784846580887 1102 98-99 0.4950413753995271 1102 100-101 0.33553638465456004 1102 102-103 0.25197314430553064 1102 104-105 0.4908883165675135 1102 106-107 0.40409749926655536 1102 108-109 0.3935453442412715 1102 110-111 0.4492650474541513 1102 112-113 0.5445569849423499 1102 114-115 0.5339430608807199 1102 116-117 0.4733121270942213 1102 118-119 0.33527022623450264 1102 120-121 0.4408967989889767 1102 122-123 0.41234076781415396 1102 124-125 0.2802655191775756 1103 1 0.4922851583106116 1103 2 0.2235772968338452 1103 3 0.45139470800505777 1103 4 0.6089786014687917 1103 5 0.49180861206448157 1103 6 0.4224411215619881 1103 7 0.5620395575035886 1103 8 0.592544996880271 1103 9 0.6790996282937058 1103 10-11 0.5908973467293208 1103 12-13 0.6794110973603864 1103 14-15 0.7103616281110163 1103 16-17 0.6563550835635255 1103 18-19 0.7637268300367808 1103 20-21 0.893140266981149 1103 22-23 0.8800707488766548 1103 24-25 1.1169129543026983 1103 26-27 1.0153509303685198 1103 28-29 0.9336495714878623 1103 30-31 0.9928573990229594 1103 32-33 1.1480440502579015 1103 34-35 1.0941233554147303 1103 36-37 1.0353578028864519 1103 38-39 1.1182337949213021 1103 40-41 1.0795280070503424 1103 42-43 1.0446144955286556 1103 44-45 0.9466838416226295 1103 46-47 0.7878599173695662 1103 48-49 0.773486693158155 1103 50-51 0.8194061005470843 1103 52-53 0.783637593483725 1103 54-55 0.7293536724218441 1103 56-57 0.7404359282220128 1103 58-59 0.7625140970729447 1103 60-61 0.7136431469603082 1103 62-63 0.7038200338770864 1103 64-65 0.6773487753065552 1103 66-67 0.6634783537066582 1103 68-69 0.7191369120575573 1103 70-71 0.7409057309834282 1103 72-73 0.6728550493087795 1103 74-75 0.7114189508738349 1103 76-77 0.6771527494898173 1103 78-79 0.642539426551366 1103 80-81 0.6112632863767189 1103 82-83 0.6052235812584463 1103 84-85 0.5577824019683071 1103 86-87 0.6402851688951934 1103 88-89 0.598089529315665 1103 90-91 0.6141522685347596 1103 92-93 0.6529132935373703 1103 94-95 0.6499233508354507 1103 96-97 0.668049629088376 1103 98-99 0.565913447089958 1103 100-101 0.5766293706183561 1103 102-103 0.4893897973328727 1103 104-105 0.6281931271306611 1103 106-107 0.7195467358387759 1103 108-109 0.6826930148050394 1103 110-111 0.6958026647122288 1103 112-113 0.7763300329944975 1103 114-115 0.7270657388525201 1103 116-117 0.7603328652865784 1103 118-119 0.6867945411392391 1103 120-121 0.7078992872297363 1103 122-123 0.8545223323677398 1103 124-125 0.9329042739018654 1104 1 0.4115184404572858 1104 2 0.4479324698072915 1104 3 0.2923217691002833 1104 4 0.33933702250609343 1104 5 0.29952208747199904 1104 6 0.2927128582045597 1104 7 0.4188495676160784 1104 8 0.33078116920035683 1104 9 0.5574012716766887 1104 10-11 0.5274797322772073 1104 12-13 0.5185639217818263 1104 14-15 0.6087291951218461 1104 16-17 0.5795600132963088 1104 18-19 0.717816586671276 1104 20-21 0.6842987084098766 1104 22-23 0.7242270169532432 1104 24-25 0.9284049569078121 1104 26-27 0.9206271354397444 1104 28-29 0.7049388510044565 1104 30-31 0.7662738638560569 1104 32-33 0.8802085695819031 1104 34-35 0.8467001145919184 1104 36-37 0.8566300201842125 1104 38-39 0.9064591472977312 1104 40-41 0.8834516411364888 1104 42-43 0.749809915155204 1104 44-45 0.6480602893102798 1104 46-47 0.6276261753752905 1104 48-49 0.5766985778258871 1104 50-51 0.5548831872356459 1104 52-53 0.6221357592951975 1104 54-55 0.6903001782042679 1104 56-57 0.6442042336939977 1104 58-59 0.6336946049444592 1104 60-61 0.5223391304723819 1104 62-63 0.5733680567404562 1104 64-65 0.5717086716015558 1104 66-67 0.5828801772833145 1104 68-69 0.5915216588500698 1104 70-71 0.5549980313450646 1104 72-73 0.6467272262398325 1104 74-75 0.6618655596538048 1104 76-77 0.5239277820536863 1104 78-79 0.6521872529911406 1104 80-81 0.5988252929768869 1104 82-83 0.5872858833263024 1104 84-85 0.631120942399523 1104 86-87 0.5538555370229687 1104 88-89 0.6138069279931386 1104 90-91 0.5973957911338772 1104 92-93 0.6187090067589267 1104 94-95 0.609986444631879 1104 96-97 0.6352541457906256 1104 98-99 0.6004154123662637 1104 100-101 0.660821536979153 1104 102-103 0.5744303929291554 1104 104-105 0.559243816485921 1104 106-107 0.5413646320255765 1104 108-109 0.5915187336030279 1104 110-111 0.647397331936542 1104 112-113 0.68722896743812 1104 114-115 0.7804589095953922 1104 116-117 0.7504415901146047 1104 118-119 0.5694105070064772 1104 120-121 0.5336499468301525 1104 122-123 0.5688382362283768 1104 124-125 0.6092761179115698 1105 1 0.27643611865050843 1105 2 0.2967642129648951 1105 3 0.2957537220054114 1105 4 0.28226659526841047 1105 5 0.28539331805908574 1105 6 0.24497353300166935 1105 7 0.15344264922507023 1105 8 0.09958752348522637 1105 9 0.18377313109668592 1105 10-11 0.2356088314689373 1105 12-13 0.1631070296092645 1105 14-15 0.21511064577997274 1105 16-17 0.202776954505552 1105 18-19 0.35942138567006054 1105 20-21 0.48354533790644183 1105 22-23 0.3833492676015382 1105 24-25 0.695572274597474 1105 26-27 0.749390633840008 1105 28-29 0.6423361791814983 1105 30-31 0.5912295434257544 1105 32-33 0.879766901515552 1105 34-35 0.8816205682528775 1105 36-37 0.8255283950505543 1105 38-39 0.8425944569825745 1105 40-41 0.7648764392749854 1105 42-43 0.7064443840064669 1105 44-45 0.630559096166408 1105 46-47 0.567313776510872 1105 48-49 0.4654859186205442 1105 50-51 0.427548363411276 1105 52-53 0.4288406902055115 1105 54-55 0.5013381928947851 1105 56-57 0.5348459402686814 1105 58-59 0.5589415806063656 1105 60-61 0.4523142587993547 1105 62-63 0.4075764902705714 1105 64-65 0.5290893674553558 1105 66-67 0.6038119151153012 1105 68-69 0.5454486384447534 1105 70-71 0.6148628732355803 1105 72-73 0.6203093309251457 1105 74-75 0.5433539835977967 1105 76-77 0.5203340122106219 1105 78-79 0.4834668511525777 1105 80-81 0.417565486665616 1105 82-83 0.4944393185565126 1105 84-85 0.5118558422164021 1105 86-87 0.4042269734657822 1105 88-89 0.4094908964408823 1105 90-91 0.463330330037536 1105 92-93 0.5095330134282428 1105 94-95 0.5671450262552113 1105 96-97 0.5001271450982934 1105 98-99 0.4889090759017378 1105 100-101 0.5285807410856158 1105 102-103 0.3888488965935899 1105 104-105 0.5502156091308272 1105 106-107 0.5533580323524916 1105 108-109 0.5102726766106933 1105 110-111 0.49475313563415924 1105 112-113 0.5595616763765605 1105 114-115 0.5877368568143915 1105 116-117 0.5770471879564951 1105 118-119 0.4589928503205698 1105 120-121 0.49171132358504366 1105 122-123 0.5571104768399238 1105 124-125 0.5039645281318457 1106 1 0.5052666580689547 1106 2 0.570347146263245 1106 3 0.5126923288636434 1106 4 0.5860194446661211 1106 5 0.5131119503288915 1106 6 0.5146881820829563 1106 7 0.4488150802457156 1106 8 0.5910657957199348 1106 9 0.5885800376050909 1106 10-11 0.5491972121718689 1106 12-13 0.447656150175348 1106 14-15 0.6222803248364528 1106 16-17 0.564710628798025 1106 18-19 0.6144688557376696 1106 20-21 0.6914159694026694 1106 22-23 0.5978130913130144 1106 24-25 0.8157803249402704 1106 26-27 0.748949765428705 1106 28-29 0.735639199320012 1106 30-31 0.7876515221960645 1106 32-33 0.9515093885463202 1106 34-35 0.9532479701206213 1106 36-37 0.7436320085920443 1106 38-39 0.9129921536877745 1106 40-41 0.799536183871524 1106 42-43 0.7381181237787189 1106 44-45 0.6766766568962836 1106 46-47 0.6104814434759902 1106 48-49 0.5381083339066564 1106 50-51 0.5564461574173976 1106 52-53 0.5715746265714401 1106 54-55 0.5810076904916812 1106 56-57 0.5383250810979519 1106 58-59 0.6136419986747796 1106 60-61 0.5916884800604336 1106 62-63 0.5513417319711138 1106 64-65 0.576406309703458 1106 66-67 0.5246867962622872 1106 68-69 0.47162014994157175 1106 70-71 0.46967043082820226 1106 72-73 0.4669622999053189 1106 74-75 0.47260880083725 1106 76-77 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124-125 -2.9976200378623936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52565.0 3 9470.0 4 6483.0 5 5270.0 6 5435.0 7 6200.0 8 6244.0 9 6803.0 10 7515.0 11 8484.0 12 8975.0 13 8725.0 14 8670.0 15 8333.0 16 7979.0 17 7205.0 18 6565.0 19 6157.0 20 6046.0 21 6338.0 22 7208.0 23 9851.0 24 15177.0 25 21638.0 26 23751.0 27 23015.0 28 26953.0 29 32062.0 30 37161.0 31 44819.0 32 56952.0 33 74494.0 34 105554.0 35 162753.0 36 314718.0 37 536654.0 38 206442.0 39 798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.061222025630585 18.334409262131736 12.239215248073522 24.36515346416416 2 16.019202328171094 20.52442096222388 39.136287073323516 24.320089636281512 3 19.057177622013274 25.278115181250087 28.847785085931104 26.816922110805535 4 12.564843177070786 16.653385188460206 36.550186779060624 34.231584855408386 5 13.575188682542086 38.916416307763015 33.70028518147093 13.808109828223964 6 32.09287481303411 36.77874564275515 17.34556590461855 13.782813639592206 7 28.58343503798444 33.218203315448136 20.49508377534722 17.703277871220212 8 26.14273269322167 35.894873778885206 19.69560647422388 18.266787053669244 9 26.737096482249296 16.89934509903961 18.928511320249427 37.435047098461666 10-11 24.859366320797484 27.54408886021421 27.2081082060946 20.388436612893706 12-13 25.03473308320221 25.07683619586866 27.22345982262178 22.664970898307356 14-15 22.50239769820972 26.453121559594177 24.64090102640538 26.40357971579072 16-17 22.786713146993904 29.25788933691436 25.377268939396945 22.578128576694787 18-19 22.753025680273595 28.071363527019745 26.942097319818764 22.233513472887896 20-21 23.79819423463517 27.300477112469178 26.574010655899965 22.327317996995685 22-23 23.338533177990843 27.198769399505284 26.430332227197397 23.032365195306483 24-25 23.11912014163264 27.265315087633873 26.83374836918696 22.781816401546525 26-27 22.962085044641796 27.38502892042925 26.69133032915879 22.961555705770166 28-29 22.832140710682953 27.593633382530836 26.657769302080876 22.916456604705335 30-31 23.14447426384871 27.215109642988423 26.59481251751607 23.045603575646794 32-33 22.860189153935863 27.283837021382563 26.735439330776455 23.12053449390512 34-35 23.182326355473823 27.429017139215233 26.474206481543238 22.914450023767703 36-37 23.389403634177018 27.15694567366937 26.425806806777135 23.02784388537648 38-39 22.98645997748491 27.31271329578701 26.615237892051987 23.085588834676095 40-41 23.14120842987214 27.160733637582187 26.677049412430982 23.021008520114695 42-43 23.04306681993992 27.223494206736827 26.48996843502506 23.2434705382982 44-45 23.02696777939288 27.36029926034364 26.484201690590186 23.128531269673296 46-47 23.112458965636325 27.437484204829783 26.20419731425872 23.245859515275168 48-49 23.056961568069955 27.307452000113265 26.465335260218094 23.170251171598686 50-51 22.96888264596481 27.3473770897859 26.444511248907077 23.239229015342207 52-53 23.146196995025228 27.2423718537858 26.452399621367995 23.159031529820975 54-55 22.878970360061174 27.156901584902187 26.552940295375233 23.41118775966141 56-57 22.979919202590004 27.48411282422038 26.37030364392826 23.165664329261354 58-59 22.93634746893062 27.22730929646645 26.453327122986046 23.38301611161688 60-61 22.975739442164645 27.221079014544692 26.619653261803087 23.18352828148758 62-63 22.870932252261696 27.201808773014918 26.578329393911243 23.348929580812143 64-65 22.740486282405175 27.137875635149793 26.50948057244128 23.61215751000375 66-67 22.747006112814628 27.537649026154714 26.225295286868818 23.49004957416184 68-69 22.812116996914405 27.1788186242946 26.55762417471147 23.45144020407953 70-71 22.699773872106086 27.026239568544273 26.66763698335598 23.60634957599366 72-73 22.758209685140702 27.150054819004666 26.58889768945107 23.50283780640357 74-75 22.596967576739424 27.346402067060925 26.475802960232787 23.58082739596686 76-77 22.718387305145892 27.1582307841109 26.553322023214587 23.570059887528622 78-79 22.80295564416678 27.295355885089567 26.53781461012532 23.363873860618334 80-81 22.73216122078385 27.18871350776883 26.593395077508365 23.48573019393895 82-83 22.84843201886701 27.21581995897188 26.5476046820321 23.388143340129012 84-85 22.81082068172465 27.106090901030967 26.507017987272746 23.576070429971644 86-87 22.705098076577006 26.976536780039588 26.645654037918003 23.6727111054654 88-89 22.76389868386407 26.953463539978273 26.59017985559156 23.692457920566095 90-91 22.697002903761977 27.106788737754012 26.733980948378772 23.462227410105243 92-93 22.700253782255352 26.865458356157855 26.78090837640222 23.653379485184566 94-95 22.568302511508566 26.758648758217944 26.833114399760355 23.83993433051313 96-97 22.76400819294708 26.723720910539157 26.768125836892608 23.744145059621154 98-99 22.363587323779257 26.782588579204408 26.735738431123536 24.118085665892803 100-101 22.24997373207992 26.89027790533121 26.914177080981684 23.94557128160719 102-103 22.469159326169486 26.64605108377363 26.919558207264675 23.965231382792208 104-105 22.255481335682468 26.772324782918115 26.86381822209483 24.10837565930458 106-107 22.160826726788017 26.960846209578175 26.801277922795734 24.07704914083807 108-109 22.234086385753265 26.967067287272684 26.602965370491365 24.19588095648269 110-111 22.04541148301555 27.04735915623409 26.879345878183624 24.027883482566736 112-113 22.216996487847744 27.03049776222703 26.845309146152648 23.907196603772586 114-115 21.989720191958224 27.21925554114054 26.747036620459504 24.043987646441735 116-117 21.86499288721396 27.156386773527462 26.896630421861158 24.08198991739743 118-119 21.90677634155895 27.10589734908084 26.774934108758725 24.212392200601485 120-121 21.769712595762336 27.14228527207935 26.96626564181065 24.121736490347665 122-123 21.947636717819503 27.0715310384255 26.90010110581173 24.080731137943264 124-125 21.923852259750078 27.159819043730742 26.745149714714085 24.17117898180509 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1468.0 1 1175.5 2 796.0 3 614.0 4 463.0 5 369.5 6 323.5 7 312.0 8 342.0 9 366.5 10 347.0 11 348.0 12 364.0 13 392.0 14 443.0 15 508.0 16 584.0 17 691.0 18 864.5 19 1094.5 20 1431.5 21 1942.0 22 2542.5 23 3456.5 24 4602.0 25 6256.0 26 8993.0 27 12125.0 28 14742.5 29 17466.5 30 20847.0 31 24908.0 32 29266.0 33 32401.0 34 36277.0 35 39891.5 36 43368.0 37 47212.0 38 48455.5 39 48860.0 40 50272.5 41 52895.0 42 55469.0 43 58718.5 44 61887.0 45 64802.5 46 66968.0 47 67925.0 48 70278.5 49 72505.0 50 72057.0 51 70434.0 52 69696.5 53 69939.5 54 69911.5 55 66260.5 56 59875.5 57 54411.0 58 50749.5 59 45680.5 60 39008.5 61 32479.0 62 26410.5 63 20632.5 64 15360.5 65 11632.0 66 8693.0 67 7105.5 68 5592.0 69 3954.0 70 3012.5 71 2216.0 72 1799.5 73 1495.5 74 1084.0 75 767.5 76 516.5 77 309.0 78 180.5 79 82.5 80 47.0 81 33.5 82 22.0 83 17.0 84 15.0 85 17.0 86 15.5 87 9.5 88 9.0 89 6.0 90 2.0 91 3.5 92 3.0 93 5.0 94 5.0 95 3.0 96 3.5 97 1.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061975313607788886 2 0.04980253638337262 3 0.04530390132217531 4 0.04588607762421261 5 0.04445709942830287 6 0.004234009469362178 7 2.1170047346810893E-4 8 0.0 9 8.468018938724357E-4 10-11 0.003572445489774338 12-13 0.0028050312734524433 14-15 0.007938767755054085 16-17 0.0032019696612051477 18-19 0.004948498567317046 20-21 0.009526521306064902 22-23 0.016036310865209253 24-25 0.003545982930590824 26-27 0.016565562048879524 28-29 0.007330128893833271 30-31 0.006642102355061917 32-33 0.0025668682408008206 34-35 0.018206240718257365 36-37 0.015136583852969787 38-39 0.02680657245289929 40-41 0.005795300461189481 42-43 0.0013760530775427078 44-45 1.852379142845953E-4 46-47 0.0020905421754975756 48-49 0.0029902691877370384 50-51 0.0017994540244789256 52-53 0.0015083658734602761 54-55 0.005424824632620291 56-57 0.0023816303265162255 58-59 0.007515366808117867 60-61 0.015057196175419245 62-63 0.01193461419176464 64-65 0.0024080928856997385 66-67 0.003731220844875419 68-69 0.010505635995854905 70-71 0.0023551677673327117 72-73 0.0033078198979392013 74-75 8.468018938724357E-4 76-77 1.852379142845953E-4 78-79 0.0031755071020216335 80-81 0.0028050312734524433 82-83 0.0013760530775427078 84-85 0.011087812297892205 86-87 0.006456864440777322 88-89 0.0015612909918273031 90-91 8.732644530559492E-4 92-93 0.0024874805632502796 94-95 0.0 96-97 0.0016936037877448714 98-99 0.02437201700801604 100-101 0.014580870110116002 102-103 0.006059926053024617 104-105 0.013522367742775456 106-107 0.03257541035490526 108-109 9.791146897900037E-4 110-111 0.0014819033142767623 112-113 0.016248011338677357 114-115 0.0324695601181712 116-117 0.05088750130989668 118-119 0.3506024466223719 120-121 0.11497981965236666 122-123 0.018629641665193583 124-125 0.312946224904232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1889462.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.67492059686356 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2333832294547 64.39471045458436 2 8.682074762326275 12.966664869854954 3 2.457988448669316 5.50650276697167 4 0.983675746527575 2.938236330600286 5 0.5153562166540215 1.9242092278869534 6 0.27733981525322393 1.2426197209429983 7 0.17246350861572549 0.9015089168215057 8 0.12145304696514045 0.7255597310695203 9 0.08834105109987743 0.5937174878694133 >10 0.4273995212251975 5.760025401832369 >50 0.02706877949131252 1.3459528945025212 >100 0.013318569002448167 1.4849746043517404 >500 0.0 0.0 >1k 1.3730471513872267E-4 0.21531759271174078 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2100 0.11114274857075718 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0585023673405445E-4 0.0 0.0 0.0 0.0 7 1.0585023673405445E-4 0.0 0.0 5.2925118367027225E-5 0.0 8 1.0585023673405445E-4 0.0 0.0 5.2925118367027225E-5 0.0 9 1.0585023673405445E-4 0.0 0.0 5.2925118367027225E-5 0.0 10-11 1.0585023673405445E-4 0.0 0.0 5.2925118367027225E-5 0.0 12-13 1.3231279591756806E-4 0.0 0.0 5.2925118367027225E-5 0.0 14-15 1.5877535510108167E-4 0.0 0.0 5.2925118367027225E-5 0.0 16-17 1.5877535510108167E-4 0.0 0.0 1.0585023673405445E-4 0.0 18-19 1.852379142845953E-4 0.0 0.0 1.0585023673405445E-4 0.0 20-21 2.117004734681089E-4 0.0 0.0 1.3231279591756806E-4 0.0 22-23 2.646255918351361E-4 0.0 0.0 2.9108815101864974E-4 0.0 24-25 3.1755071020216335E-4 0.0 0.0 8.468018938724356E-4 0.0 26-27 3.1755071020216335E-4 0.0 0.0 0.0017994540244789256 0.0 28-29 3.4401326938567696E-4 0.0 0.0 0.004260472028545691 0.0 30-31 3.704758285691906E-4 0.0 0.0 0.011537675804011936 0.0 32-33 3.704758285691906E-4 0.0 0.0 0.02802385017534092 0.0 34-35 3.704758285691906E-4 0.0 0.0 0.05554491172619508 0.0 36-37 3.704758285691906E-4 0.0 0.0 0.09949922253001119 0.0 38-39 4.234009469362178E-4 0.0 0.0 0.15753161481945654 0.0 40-41 4.234009469362178E-4 0.0 0.0 0.22167685828029354 0.0 42-43 4.763260653032451E-4 0.0 0.0 0.2824878192840078 0.0 44-45 4.763260653032451E-4 0.0 0.0 0.3581707385488567 0.0 46-47 5.821763020372995E-4 0.0 0.0 0.45415044070746063 0.0 48-49 5.821763020372995E-4 0.0 0.0 0.5536761257966554 0.0 50-51 5.821763020372995E-4 0.0 0.0 0.6613787416735557 0.0 52-53 5.821763020372995E-4 0.0 0.0 0.7644504096933413 0.0 54-55 5.821763020372995E-4 0.0 0.0 0.8811238331334528 0.0 56-57 7.409516571383811E-4 0.0 0.0 1.029261239442762 0.0 58-59 7.409516571383811E-4 0.0 0.0 1.1888833964377161 0.0 60-61 7.409516571383811E-4 0.0 0.0 1.363324586575438 0.0 62-63 7.674142163218948E-4 0.0 0.0 1.538506728370298 0.0 64-65 7.938767755054084E-4 0.0 0.0 1.714959073005967 0.0 66-67 8.997270122394628E-4 0.0 0.0 1.9259450573761208 0.0 68-69 8.997270122394628E-4 0.0 0.0 2.1737139990113588 0.0 70-71 9.5265213060649E-4 0.0 0.0 2.446066658128081 0.0 72-73 0.0010055772489735173 0.0 0.0 2.716858026252976 0.0 74-75 0.0010055772489735173 0.0 0.0 2.9862468787411443 0.0 76-77 0.0010055772489735173 0.0 0.0 3.2952766448862163 0.0 78-79 0.0010320398081570309 0.0 0.0 3.645032289614716 0.0 80-81 0.001164352604074599 0.0 0.0 4.033873134257265 0.0 82-83 0.001164352604074599 0.0 0.0 4.441899334307861 0.0 84-85 0.0012172777224416262 0.0 0.0 4.842436630109523 0.0 86-87 0.0012702028408086534 0.0 0.0 5.263852885106978 0.0 88-89 0.0012702028408086534 0.0 0.0 5.731208142847011 0.0 90-91 0.0015348284326437895 0.0 0.0 6.242464786272494 0.0 92-93 0.0015877535510108167 0.0 0.0 6.779443037224352 0.0 94-95 0.0015877535510108167 0.0 0.0 7.324360055931265 0.0 96-97 0.0015877535510108167 0.0 0.0 7.869250612078995 0.0 98-99 0.001640678669377844 0.0 0.0 8.442694269585733 0.0 100-101 0.001640678669377844 0.0 0.0 9.082955889030845 0.0 102-103 0.001640678669377844 0.0 0.0 9.775454600304213 0.0 104-105 0.001931766820396494 0.0 0.0 10.46109950874905 0.0 106-107 0.0019582293795800073 0.0 0.0 11.126209471267483 0.0 108-109 0.0019582293795800073 0.0 0.0 11.810928190140897 0.0 110-111 0.0019582293795800073 0.0 0.0 12.5624384083935 0.0 112-113 0.0019582293795800073 0.0 0.0 13.35104913462139 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2230 0.0 53.362514 1 GTATCAA 3570 0.0 40.832787 1 GTACATG 9430 0.0 36.658577 1 TACATGG 9380 0.0 35.64406 2 ATCAACG 4085 0.0 34.07646 3 TCAACGC 4145 0.0 33.15176 4 ACATGGG 9710 0.0 32.89923 3 CAACGCA 4245 0.0 32.232376 5 AACGCAG 4400 0.0 31.221376 6 TATCAAC 4735 0.0 29.902716 2 CATGGGG 4425 0.0 27.558767 4 ACGCAGA 5210 0.0 26.253237 7 CATGGGA 5665 0.0 26.251814 4 CGCAGAG 5270 0.0 25.615803 8 AGTACAT 6465 0.0 23.83334 2 GAGTACA 6720 0.0 23.72885 1 GTATAGG 610 0.0 22.434128 1 TATACGA 135 0.002558443 22.033243 5 ATGGGAG 2780 0.0 21.613186 5 ATGGGGA 2635 0.0 21.44792 5 >>END_MODULE