FastQCFastQC Report
Thu 26 May 2016
SRR1364188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889462
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62100.3286649850592391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58350.30881806567160386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.17216541004793956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40450.072.853171
GTATCAA71600.050.3041881
ATCAACG92800.038.7896843
TCAACGC93450.038.456214
CAACGCA94900.037.8656235
TATCAAC97800.036.748652
AACGCAG98450.036.6805536
TACATGG95850.033.7714842
GTACATG97950.033.367961
TGGTATC18750.031.735262
GTGGTAT20150.031.3178981
ACGCAGA114750.031.21017
ACATGGG99500.031.1546943
CGCAGAG115400.030.82818
GCAGAGT130300.027.1658929
CATGGGA56500.026.3269424
GAGTACT80950.026.19991112-13
CAGAGTA124150.025.51668710-11
CATGGGG46450.024.8499134
AGAGTAC119900.024.46127910-11
Produced by FastQC (version 0.11.5)