FastQCFastQC Report
Thu 26 May 2016
SRR1364187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240183
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.049.554331
GTATCAA32900.036.88991
ATCAACG33600.033.4586523
CAACGCA33700.033.3623475
TCAACGC33900.033.164044
GTACATG74200.032.2325971
AACGCAG36000.031.3869936
TACATGG75450.030.0382
ACATGGG73650.029.397783
TATCAAC40550.028.7521632
ACGCAGA42200.026.3499227
CATGGGG47300.024.6490544
CGCAGAG44000.024.3254188
GAGTACA56150.023.8399851
AGTACAT59850.020.5736582
ATGGGGA32250.018.630155
CATGGGA35450.018.2900774
GCAGAGT58300.018.157259
CAGAGTA55500.017.680310-11
TAGCCCG1700.00970751417.49554
Produced by FastQC (version 0.11.5)