FastQCFastQC Report
Thu 26 May 2016
SRR1364187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240183
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.24605132705676278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55020.24560493495397476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31150.13905114001847171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.065.589211
GTATCAA67700.047.9210741
ATCAACG79250.040.170613
TCAACGC80500.039.4729234
CAACGCA81400.039.02695
AACGCAG83500.038.187886
TATCAAC84750.037.634722
TGGTATC19700.033.2269172
ACGCAGA98150.032.304637
CGCAGAG100000.031.7664818
GTGGTAT22850.028.6566371
GTACATG74250.028.3006881
GCAGAGT116550.027.1025459
TACATGG75850.026.5170172
ACATGGG75050.026.244073
GAGTACT76400.026.16216912-13
CAGAGTA111800.024.9816810-11
GTACTTT81550.024.40057214-15
CGTTCGG1351.718945E-924.23576734-35
AGAGTAC104200.023.92071510-11
Produced by FastQC (version 0.11.5)