FastQCFastQC Report
Thu 26 May 2016
SRR1364186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586822
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49800.3138348220531351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.051.6738051
GTATCAA44100.047.8887831
GTACATG118700.040.7960131
TACATGG118250.039.4860882
TCAACGC52100.038.4728244
ATCAACG52350.038.175483
CAACGCA53350.037.799145
ACATGGG116900.037.2950633
AACGCAG55000.036.440816
CATGGGG36150.034.881044
TATCAAC59850.034.2870832
CATGGGA82350.033.079874
ACGCAGA64350.030.6838847
CGCAGAG64100.030.4314698
ATGGGAG33750.027.1433565
ATGGGGA22800.027.1340835
GCAGAGT71350.026.422419
ATGGGAT30450.026.1778585
GAGTACA81550.024.2920551
AGTACAT80850.023.6892572
Produced by FastQC (version 0.11.5)