##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1586822 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.754686410952203 31.0 31.0 34.0 26.0 34.0 2 30.739797532426447 33.0 31.0 34.0 26.0 34.0 3 30.858843020830314 33.0 31.0 34.0 26.0 34.0 4 34.06911613274835 37.0 35.0 37.0 30.0 37.0 5 34.32071208995086 37.0 35.0 37.0 31.0 37.0 6 34.30404355371932 37.0 35.0 37.0 30.0 37.0 7 34.23501501743737 37.0 35.0 37.0 30.0 37.0 8 34.145814086268025 37.0 35.0 37.0 30.0 37.0 9 35.52761368319824 39.0 37.0 39.0 29.0 39.0 10-11 35.56237309540705 39.0 37.0 39.0 30.0 39.0 12-13 35.50697526250581 39.0 37.0 39.0 29.5 39.0 14-15 36.80211674655381 40.0 38.0 41.0 29.5 41.0 16-17 36.862985577462375 40.0 38.0 41.0 30.0 41.0 18-19 36.791693082147845 40.0 38.0 41.0 28.5 41.0 20-21 36.80579233209522 40.0 37.5 41.0 28.5 41.0 22-23 36.899755297065454 40.0 37.0 41.0 28.5 41.0 24-25 36.808178548066515 40.0 37.0 41.0 28.5 41.0 26-27 36.874735792672396 40.0 37.0 41.0 29.5 41.0 28-29 36.97336279683543 40.0 37.0 41.0 30.0 41.0 30-31 36.87283891955116 40.0 37.0 41.0 30.0 41.0 32-33 36.68925090526852 40.0 37.0 41.0 29.5 41.0 34-35 36.6357792493424 40.0 37.0 41.0 29.5 41.0 36-37 36.65155984729226 40.0 37.0 41.0 29.0 41.0 38-39 36.47582369036981 40.0 36.5 41.0 28.5 41.0 40-41 36.4704768398724 40.0 36.0 41.0 29.0 41.0 42-43 36.39976916125438 40.0 36.0 41.0 28.0 41.0 44-45 36.404249499943916 40.0 36.0 41.0 28.5 41.0 46-47 36.342647127403076 40.0 36.0 41.0 28.5 41.0 48-49 36.2583263907357 40.0 35.5 41.0 28.5 41.0 50-51 36.16328643036207 40.0 35.0 41.0 29.0 41.0 52-53 36.0305346157288 40.0 35.0 41.0 29.0 41.0 54-55 35.856986794990235 39.5 35.0 41.0 28.0 41.0 56-57 35.68378085254679 39.0 35.0 41.0 28.0 41.0 58-59 35.467552441294615 39.0 35.0 41.0 28.0 41.0 60-61 35.229710389697146 38.5 35.0 41.0 28.0 41.0 62-63 34.94530735016278 38.0 34.0 40.0 27.5 41.0 64-65 34.645328209465205 37.5 34.0 40.0 27.0 41.0 66-67 34.339844985764 37.0 34.0 40.0 26.5 41.0 68-69 34.09677109341817 37.0 34.0 39.5 26.0 41.0 70-71 33.91698281218687 36.0 34.0 39.0 26.0 41.0 72-73 33.60435070852307 36.0 34.0 39.0 26.0 41.0 74-75 33.26598982116457 35.0 34.0 38.5 26.0 40.5 76-77 32.934941663274145 35.0 33.5 37.0 26.0 39.5 78-79 32.59352340716224 35.0 33.0 37.0 26.0 39.0 80-81 32.28815676868609 35.0 33.0 36.5 25.0 39.0 82-83 32.008226505556394 35.0 33.0 36.0 25.0 37.5 84-85 31.761673331980525 35.0 33.0 36.0 24.5 37.0 86-87 31.546653941021738 35.0 33.0 35.0 24.0 37.0 88-89 31.363556529969966 35.0 33.0 35.0 24.0 36.0 90-91 31.207321930248007 35.0 33.0 35.0 23.0 36.0 92-93 31.074211852369075 35.0 33.0 35.0 23.0 36.0 94-95 30.937941054510212 35.0 32.0 35.0 20.5 36.0 96-97 30.84300633593434 35.0 32.0 35.0 20.0 35.0 98-99 30.76374476784416 35.0 32.0 35.0 20.0 35.0 100-101 30.667297907389738 35.0 32.0 35.0 19.0 35.0 102-103 29.734179700054575 34.0 30.0 35.0 17.0 35.0 104-105 30.377806395424315 35.0 31.5 35.0 18.0 35.0 106-107 30.473356179836173 35.0 32.0 35.0 17.5 35.0 108-109 30.43353444809815 35.0 32.0 35.0 16.5 35.0 110-111 30.369856543456038 35.0 32.0 35.0 15.0 35.0 112-113 30.502985211951938 35.0 32.5 35.0 13.0 35.0 114-115 30.487103468441955 35.0 33.0 35.0 8.5 35.0 116-117 30.397583030736907 35.0 32.5 35.0 2.0 35.0 118-119 30.16214011401405 35.0 32.0 35.0 2.0 35.0 120-121 30.106909911760738 35.0 32.0 35.0 2.0 35.0 122-123 29.976076711817708 35.0 32.0 35.0 2.0 35.0 124-125 29.144040100275898 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9156155614571659 1101 2 0.9367563430316856 1101 3 0.8932753277676291 1101 4 0.936789371626034 1101 5 0.7600573131879855 1101 6 0.8677299804745999 1101 7 0.8939032255121617 1101 8 0.8981159960785661 1101 9 1.051334256750053 1101 10-11 0.9915655869821904 1101 12-13 1.0061423790116706 1101 14-15 1.1324420172216634 1101 16-17 1.1502229780758242 1101 18-19 1.2979526343102208 1101 20-21 1.2672440016772129 1101 22-23 1.174813516441823 1101 24-25 1.3748499445392284 1101 26-27 1.3872156478813338 1101 28-29 1.1921834227622696 1101 30-31 1.3206549887281156 1101 32-33 1.4809856558943864 1101 34-35 1.356620753493516 1101 36-37 1.324397754356312 1101 38-39 1.378851289990962 1101 40-41 1.3332133588106387 1101 42-43 1.2932172147935859 1101 44-45 1.203274400606361 1101 46-47 1.1321559055513575 1101 48-49 1.0955855478829122 1101 50-51 1.0173623300148549 1101 52-53 1.000658003871905 1101 54-55 1.033104625503249 1101 56-57 0.9912157273020838 1101 58-59 1.0389189554680485 1101 60-61 1.0346219775132681 1101 62-63 0.9670975647419837 1101 64-65 0.9695920681325916 1101 66-67 1.0012157815244436 1101 68-69 0.8993069750793197 1101 70-71 0.9378969982933967 1101 72-73 0.9155577881535706 1101 74-75 0.9201980153799809 1101 76-77 0.9088486612392614 1101 78-79 0.8634087710408025 1101 80-81 0.9623687207231271 1101 82-83 1.02368150760946 1101 84-85 0.9441888569406842 1101 86-87 0.9860290008124011 1101 88-89 0.9228359530924273 1101 90-91 0.9417621707456867 1101 92-93 0.9585394739700028 1101 94-95 0.9882366057405285 1101 96-97 0.9158039815154844 1101 98-99 0.9623720917447898 1101 100-101 0.9365406358354562 1101 102-103 0.7221173191845729 1101 104-105 0.8173372871997877 1101 106-107 0.8703415105627599 1101 108-109 0.8815098005934381 1101 110-111 0.9189686751698964 1101 112-113 0.798337070080084 1101 114-115 0.723708877472788 1101 116-117 0.7670278979207197 1101 118-119 0.7296529514471644 1101 120-121 0.7304321695778491 1101 122-123 0.6645608607458016 1101 124-125 0.6250361671419817 1102 1 0.3105488477676346 1102 2 0.31200300314873886 1102 3 0.21175447396675295 1102 4 0.3427520388301062 1102 5 0.1506367860280804 1102 6 0.28433576932513915 1102 7 0.22132091450629332 1102 8 0.27798937022102876 1102 9 0.29399323181953463 1102 10-11 0.3092827108622487 1102 12-13 0.24603036383000898 1102 14-15 0.4307580108735749 1102 16-17 0.4026140067849653 1102 18-19 0.4601710219297743 1102 20-21 0.519689610497359 1102 22-23 0.48335488396749327 1102 24-25 0.6712094511350344 1102 26-27 0.6453816721820758 1102 28-29 0.5060820381068396 1102 30-31 0.5701175845581901 1102 32-33 0.7276415744776514 1102 34-35 0.6036356412684043 1102 36-37 0.5654424176809982 1102 38-39 0.6606924870587463 1102 40-41 0.5848507621408743 1102 42-43 0.5321479484342433 1102 44-45 0.4052816891504136 1102 46-47 0.4487478394506468 1102 48-49 0.37020117794731533 1102 50-51 0.28245582994347984 1102 52-53 0.237764502263893 1102 54-55 0.28332294594728324 1102 56-57 0.36858261480826826 1102 58-59 0.37060438929596273 1102 60-61 0.24421590148737948 1102 62-63 0.3739062236666726 1102 64-65 0.3960537654900733 1102 66-67 0.42923930975075564 1102 68-69 0.43529835981872367 1102 70-71 0.3941993217267381 1102 72-73 0.3698385482445872 1102 74-75 0.4141858062289927 1102 76-77 0.3048000765435148 1102 78-79 0.29145329161750055 1102 80-81 0.3371304575462304 1102 82-83 0.3290656256368507 1102 84-85 0.30339526212663515 1102 86-87 0.3103608142995604 1102 88-89 0.35742588851995905 1102 90-91 0.295705793689482 1102 92-93 0.3636078670475662 1102 94-95 0.2587566282443312 1102 96-97 0.27932221547103 1102 98-99 0.24344706802343552 1102 100-101 0.26438656877749267 1102 102-103 0.22400033301433453 1102 104-105 0.2433560459640951 1102 106-107 0.2869305055329967 1102 108-109 0.25868985618118145 1102 110-111 0.2780155384928982 1102 112-113 0.2856052095700541 1102 114-115 0.29589527275034655 1102 116-117 0.33694711935643085 1102 118-119 0.18623237390561798 1102 120-121 0.24300238898989335 1102 122-123 0.27014188701982533 1102 124-125 0.2643686943690646 1103 1 0.4075955952266739 1103 2 0.033688939703019116 1103 3 0.3059584484183837 1103 4 0.3923299461586325 1103 5 0.30459021824030685 1103 6 0.28801026815688857 1103 7 0.3286385804282759 1103 8 0.28549550802638635 1103 9 0.4335981899761592 1103 10-11 0.2929787471369707 1103 12-13 0.30084897191883186 1103 14-15 0.52958741310362 1103 16-17 0.4119922498464845 1103 18-19 0.4995433895676058 1103 20-21 0.5778670891183069 1103 22-23 0.5313868096063175 1103 24-25 0.6887458865611151 1103 26-27 0.6561761022573123 1103 28-29 0.6024380236923079 1103 30-31 0.6678682851894635 1103 32-33 0.7952187681280378 1103 34-35 0.7307446843947076 1103 36-37 0.5816318000528469 1103 38-39 0.7309717786263192 1103 40-41 0.6319293516617392 1103 42-43 0.6410067817033536 1103 44-45 0.6361911357956558 1103 46-47 0.537391248220878 1103 48-49 0.4416882917816807 1103 50-51 0.45285784873212265 1103 52-53 0.5694728986891846 1103 54-55 0.4431095319178624 1103 56-57 0.4518633273771897 1103 58-59 0.37649204141234094 1103 60-61 0.44138036925121327 1103 62-63 0.43612601689257957 1103 64-65 0.38060877676814897 1103 66-67 0.4270848914456309 1103 68-69 0.5379642153649513 1103 70-71 0.4759028556660212 1103 72-73 0.4105785743229191 1103 74-75 0.4996976777237876 1103 76-77 0.39063845675218545 1103 78-79 0.36979362211634026 1103 80-81 0.39056137952942294 1103 82-83 0.3814083136229627 1103 84-85 0.35712342162080546 1103 86-87 0.3562203159919939 1103 88-89 0.33078148722486134 1103 90-91 0.29574132353773663 1103 92-93 0.33593820335635627 1103 94-95 0.3670557460449366 1103 96-97 0.3210329093006017 1103 98-99 0.2895117569966814 1103 100-101 0.3151735501018891 1103 102-103 0.24955640402550117 1103 104-105 0.2770115743603405 1103 106-107 0.3090696450405126 1103 108-109 0.3151811594564542 1103 110-111 0.34682212266484314 1103 112-113 0.3683083936029199 1103 114-115 0.37885178748984316 1103 116-117 0.5239301191424772 1103 118-119 0.49091661369300255 1103 120-121 0.4775494566027163 1103 122-123 0.41283409050013375 1103 124-125 0.4309605461520185 1104 1 0.5415761071926575 1104 2 0.5660532478051366 1104 3 0.5585596400665409 1104 4 0.5759821783019845 1104 5 0.4574960888631665 1104 6 0.5502538800766388 1104 7 0.5678823966538715 1104 8 0.4810953681640129 1104 9 0.6290775430348319 1104 10-11 0.6180972055955891 1104 12-13 0.6224674413157842 1104 14-15 0.607033604838918 1104 16-17 0.636935861803309 1104 18-19 0.8309842055748007 1104 20-21 0.8357242433640266 1104 22-23 0.8181620475892615 1104 24-25 1.0320058514768746 1104 26-27 1.115154565602957 1104 28-29 0.8575578132906614 1104 30-31 0.8803947550328459 1104 32-33 1.016087294163313 1104 34-35 1.0325008062470147 1104 36-37 1.0300584781695363 1104 38-39 1.1075021721801903 1104 40-41 1.030725467403414 1104 42-43 0.8879402473349245 1104 44-45 0.8266844621136187 1104 46-47 0.7323367549197712 1104 48-49 0.6470427364720805 1104 50-51 0.699939352675294 1104 52-53 0.7173013425868433 1104 54-55 0.7584174163808655 1104 56-57 0.6971137726045029 1104 58-59 0.7798418415745232 1104 60-61 0.8004116517765425 1104 62-63 0.8051784827084276 1104 64-65 0.8287449401268034 1104 66-67 0.7840643092073307 1104 68-69 0.7610045570562889 1104 70-71 0.6953157488852568 1104 72-73 0.6900209565513435 1104 74-75 0.7028617672075228 1104 76-77 0.7391095185530361 1104 78-79 0.7329691430869829 1104 80-81 0.707455415244759 1104 82-83 0.6917334157666133 1104 84-85 0.8201176443260394 1104 86-87 0.7810681155512711 1104 88-89 0.8412683840266801 1104 90-91 0.8746919998049201 1104 92-93 0.8446511432581794 1104 94-95 0.875273309154494 1104 96-97 0.8301886663687164 1104 98-99 0.7466612936747694 1104 100-101 0.8278745327351515 1104 102-103 0.8697682406386669 1104 104-105 0.9064858631553712 1104 106-107 0.8552060583123087 1104 108-109 0.8504380082445415 1104 110-111 0.8173662065644685 1104 112-113 0.8548369072463622 1104 114-115 0.8797440033628874 1104 116-117 0.9212025619173758 1104 118-119 0.9440073792540815 1104 120-121 0.7885072209108905 1104 122-123 0.8126304149971482 1104 124-125 0.7072608453432849 1105 1 0.46854955499243545 1105 2 0.43516571094324874 1105 3 0.5374248830313206 1105 4 0.5140542013814908 1105 5 0.35655197345398904 1105 6 0.3273814676831819 1105 7 0.2473065146235598 1105 8 0.18299778362717234 1105 9 0.3192037936476808 1105 10-11 0.3184584048454724 1105 12-13 0.33168767865762305 1105 14-15 0.3631515512515051 1105 16-17 0.3989788070884046 1105 18-19 0.5564236749672489 1105 20-21 0.5900143709967409 1105 22-23 0.5692273507842174 1105 24-25 0.7823015467645149 1105 26-27 0.7288030308243876 1105 28-29 0.6552953260750272 1105 30-31 0.6367480210654932 1105 32-33 0.7806089151990676 1105 34-35 0.7500745263919839 1105 36-37 0.6635734476980062 1105 38-39 0.6560287512948619 1105 40-41 0.6674825375395699 1105 42-43 0.5895640618259534 1105 44-45 0.4644282743351056 1105 46-47 0.4284949348043483 1105 48-49 0.36585743118215674 1105 50-51 0.23683161338761494 1105 52-53 0.17992025719236437 1105 54-55 0.27044745000210924 1105 56-57 0.28930250694137527 1105 58-59 0.32374659059359345 1105 60-61 0.30966404097991074 1105 62-63 0.27469877256982755 1105 64-65 0.31396563137727895 1105 66-67 0.42334879207882636 1105 68-69 0.4360307077219474 1105 70-71 0.3946936612837959 1105 72-73 0.3925456778412624 1105 74-75 0.36543961908491696 1105 76-77 0.4065721620179801 1105 78-79 0.3654720927656854 1105 80-81 0.31076291070203155 1105 82-83 0.34789435256420376 1105 84-85 0.38595421830515164 1105 86-87 0.3102279263062897 1105 88-89 0.4015340296898877 1105 90-91 0.3552044801100891 1105 92-93 0.323262335597601 1105 94-95 0.36502744925222785 1105 96-97 0.3428759218654456 1105 98-99 0.3871702023591226 1105 100-101 0.4055069118419681 1105 102-103 0.4075314021704912 1105 104-105 0.39568516541632803 1105 106-107 0.45389336036376804 1105 108-109 0.34293687711004495 1105 110-111 0.39596114111869696 1105 112-113 0.37660524508241267 1105 114-115 0.3689180278594044 1105 116-117 0.41949785858012945 1105 118-119 0.34034126687969035 1105 120-121 0.3144302048364125 1105 122-123 0.3692009606913409 1105 124-125 0.40439231008607734 1106 1 0.32214008563245855 1106 2 0.33121488668075827 1106 3 0.4015013226994171 1106 4 0.281485288358823 1106 5 0.2539795868676151 1106 6 0.2859849178534972 1106 7 0.2527773774496893 1106 8 0.24489509374197382 1106 9 0.27698386744318526 1106 10-11 0.3509423396133684 1106 12-13 0.3913950486382376 1106 14-15 0.4462487649571969 1106 16-17 0.5046861735245685 1106 18-19 0.5519798173266466 1106 20-21 0.5893029107176488 1106 22-23 0.45799125833072196 1106 24-25 0.723949221071841 1106 26-27 0.6930460597719375 1106 28-29 0.5460118726438168 1106 30-31 0.5973062369297608 1106 32-33 0.695865037355091 1106 34-35 0.7174851441546508 1106 36-37 0.6252233550105686 1106 38-39 0.5796609469770075 1106 40-41 0.42404828182148435 1106 42-43 0.43152666076049684 1106 44-45 0.415638527728035 1106 46-47 0.4242340595371843 1106 48-49 0.3158731153855925 1106 50-51 0.32324563736938927 1106 52-53 0.34977885124973085 1106 54-55 0.3201541465367015 1106 56-57 0.36316987706377546 1106 58-59 0.25742420843779 1106 60-61 0.2938490472358808 1106 62-63 0.32130256655205613 1106 64-65 0.2904260345233425 1106 66-67 0.3203218294267245 1106 68-69 0.32077981363374164 1106 70-71 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-2.825066614846804 2216 108-109 -2.7798947845472846 2216 110-111 -2.920931426648689 2216 112-113 -2.8993293951940444 2216 114-115 -2.939230936086357 2216 116-117 -2.8708282566789194 2216 118-119 -2.7300364301779787 2216 120-121 -2.6513727003805556 2216 122-123 -2.7282415694324023 2216 124-125 -2.52717123225683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29378.0 3 6352.0 4 5099.0 5 4109.0 6 4766.0 7 4888.0 8 4458.0 9 4279.0 10 5020.0 11 6413.0 12 7183.0 13 6897.0 14 6484.0 15 6083.0 16 5376.0 17 4766.0 18 4277.0 19 3967.0 20 3788.0 21 3898.0 22 4476.0 23 6674.0 24 12024.0 25 18207.0 26 20645.0 27 16189.0 28 17388.0 29 21749.0 30 25093.0 31 30429.0 32 39957.0 33 54258.0 34 77841.0 35 125189.0 36 246561.0 37 494699.0 38 246679.0 39 1283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.900005359952324 19.523279534125557 12.56448495587498 23.012230150047134 2 15.271362404589055 21.65905648933125 39.81894461278888 23.250636493290813 3 18.221349081805005 28.034721622393533 29.07488236877727 24.669046927024194 4 11.5524121221139 17.849205563608187 37.433492235322895 33.16489007895502 5 12.517597897267999 41.346815229801905 33.202259339888926 12.933327533041176 6 30.053909205151186 38.53461999493301 18.014648884317676 13.396821915598126 7 27.435071073321854 35.170076858506235 20.898190837982067 16.49666123018984 8 25.666709939741196 37.37986995390787 19.563441898335164 17.38997820801577 9 26.71993516527561 17.870070909828815 19.337990073128125 36.072003851767455 10-11 23.96912942054327 29.408159490431245 27.162032413982384 19.4606786750431 12-13 24.215115225669635 27.27628794837336 27.095260677694256 21.41333614826275 14-15 21.856454137429992 28.020569846123205 25.01330598922349 25.109670027223313 16-17 21.806613474937066 31.03287137972574 25.649059996199842 21.511455149137355 18-19 21.869953259203918 29.53931341164326 27.46306159934633 21.1276717298065 20-21 23.43079568200955 28.46163396913272 26.902324625755675 21.205245723102056 22-23 22.78504628717747 28.702493598581842 26.585873547370493 21.9265865668702 24-25 22.190312244072892 28.67713393777428 27.28337793692853 21.849175881224298 26-27 22.142325671395223 28.921623469059426 26.858676808489783 22.077374051055564 28-29 21.948951427313656 28.895140840654328 27.107378847164043 22.048528884867977 30-31 22.094564764924186 28.52988712818934 27.3492692275817 22.026278879304776 32-33 21.94089410056473 28.665554560527962 27.071192648755748 22.32235869015156 34-35 22.338370328163133 28.7450331793703 26.869963845579782 22.04663264688679 36-37 22.520484312168314 28.4106379760239 26.784750847735378 22.284126864072405 38-39 22.14117696747232 28.655097388260458 26.965061132223433 22.238664512043794 40-41 22.224403506328418 28.48475891628764 27.251468585412397 22.039368991971546 42-43 22.218258966088005 28.575524869729836 26.88740882979156 22.3188073343906 44-45 22.053904783571383 28.664688071998246 26.972116813758866 22.309290330671505 46-47 22.23102410065652 28.76912872432107 26.662349754928606 22.337497420093804 48-49 22.154127617041738 28.7176156741744 26.8275728637484 22.30068384503546 50-51 22.05863215173512 28.59732989494839 26.81892989721712 22.52510805609937 52-53 22.42253228215454 28.587179147839347 26.745914708310487 22.244373861695625 54-55 22.003028254783054 28.467044489505884 26.982663950375695 22.54726330533537 56-57 22.04652332342224 28.923243007510834 26.719179921022153 22.311053748044777 58-59 22.233461556278026 28.43937014771951 26.75904769017505 22.568120605827417 60-61 22.072911194219646 28.71431754442747 26.925873272545115 22.286897988807766 62-63 22.01157930297285 28.621116282118507 26.97022104796095 22.397083366947683 64-65 21.824252446470073 28.533075872082765 26.91107629558299 22.731595385864168 66-67 21.95233469134896 28.93903046570304 26.507490303302063 22.601144539645937 68-69 22.044187578191718 28.592785405683063 26.8840540889739 22.478972927151318 70-71 21.738638598012574 28.712023625262795 27.080287324738965 22.469050451985662 72-73 22.029033914914937 28.69523842534969 26.794137758269184 22.48158990146619 74-75 21.810008703055647 28.93293353474511 26.729283292969054 22.52777446923019 76-77 21.96165842846884 28.710174560306474 26.85731264913911 22.470854362085575 78-79 22.18936011318626 28.882348677970587 26.744413949135502 22.183877259707643 80-81 22.094874314095335 28.991670516198596 26.708231108041293 22.205224061664776 82-83 22.096251582204193 28.890599540770843 26.70886718118566 22.304281695839304 84-85 22.057554188083962 28.711357083724494 26.7273047563494 22.503783971842147 86-87 22.106912402316393 28.4374120615129 26.84646007116065 22.609215465010056 88-89 22.124659531909156 28.425887296711714 26.848043781581133 22.601409389797993 90-91 22.03267636309325 28.63657747378458 26.95316278722426 22.377583375897913 92-93 22.08793213680153 28.441835527976302 26.853493599146194 22.616738736075973 94-95 21.976472471392505 28.23653188574396 26.93014717466735 22.85684846819618 96-97 22.212814737603654 28.32786529854462 26.80089085063127 22.65842911322046 98-99 21.795509414219346 28.434787541839224 26.620651399683563 23.149051644257863 100-101 21.632602252332457 28.64461395677173 26.887655976465062 22.83512781443075 102-103 22.08748487828735 28.517336360144284 26.761909953844704 22.63326880772366 104-105 21.840130569457674 28.596797468278634 26.770993234855794 22.792078727407894 106-107 21.703045495679078 28.82209376764136 26.783599630207146 22.691261106472417 108-109 21.767205930452178 29.110917429053444 26.233825079893847 22.88805156060053 110-111 21.590486924973114 29.190195644083992 26.621510356321227 22.597807074621663 112-113 21.78368000645423 29.205159348198645 26.493217703492533 22.517942941854592 114-115 21.585895307912896 29.442403718317173 26.3526210845428 22.61907988922713 116-117 21.675707695503906 29.432844234007277 26.377536592601373 22.51391147788744 118-119 21.64257031414543 29.48659241798185 26.18689214924201 22.68394511863071 120-121 21.488741359163512 29.496515903681964 26.46121308637177 22.553529650782757 122-123 21.70849415677879 29.503059099090358 26.188585981752876 22.599860762377983 124-125 21.681742816634713 29.578473691947078 26.20234337584581 22.5374401155724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3927.0 1 3158.5 2 2177.5 3 1716.5 4 1227.5 5 912.0 6 776.5 7 715.0 8 710.5 9 702.0 10 655.0 11 598.0 12 557.0 13 577.5 14 665.5 15 750.5 16 834.5 17 949.0 18 1197.5 19 1492.0 20 1883.0 21 2501.0 22 3321.0 23 4228.0 24 5288.5 25 6839.5 26 9554.5 27 12618.5 28 15155.0 29 17810.5 30 20647.5 31 24225.0 32 28659.5 33 32407.5 34 36639.0 35 40170.5 36 43345.5 37 47330.0 38 48854.0 39 48551.5 40 48558.0 41 50431.0 42 52686.0 43 53966.5 44 55367.5 45 57112.5 46 56885.5 47 55532.5 48 56139.5 49 56734.0 50 55581.5 51 53065.0 52 51026.0 53 50462.0 54 49022.5 55 45141.0 56 40642.0 57 36499.5 58 33560.0 59 30443.0 60 26341.5 61 22222.0 62 18254.0 63 14136.5 64 10573.0 65 8504.0 66 6309.0 67 4895.5 68 3802.0 69 2354.0 70 1741.5 71 1484.0 72 1212.5 73 1064.0 74 831.0 75 685.5 76 492.0 77 256.5 78 156.0 79 79.0 80 43.5 81 33.0 82 33.5 83 22.0 84 10.0 85 9.0 86 10.0 87 9.5 88 6.5 89 4.0 90 3.5 91 4.0 92 3.5 93 4.0 94 4.0 95 3.0 96 2.0 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.062199793045470755 2 0.049659003971459934 3 0.045436728253074384 4 0.04594088057765773 5 0.04379823319817849 6 0.005041523245833496 7 6.30190405729187E-4 8 0.0 9 0.0011343427303125366 10-11 0.004316804279244931 12-13 0.0028043473054948824 14-15 0.00822398479476589 16-17 0.0029303853866407196 18-19 0.00582926125299498 20-21 0.009957008410521155 22-23 0.016353441028672405 24-25 0.004190766198099094 26-27 0.01616438390695365 28-29 0.007404737267317947 30-31 0.006963603983307516 32-33 0.002709818744635504 34-35 0.018527597928438097 36-37 0.015250607818646325 38-39 0.02933536338669366 40-41 0.005734732692135602 42-43 0.0014809474534635894 44-45 3.4660472315105285E-4 46-47 0.0024892521026302888 48-49 0.0037181233938022033 50-51 0.0019220807374740203 52-53 0.0020166092983333983 54-55 0.00627039453700541 56-57 0.0030249139475000976 58-59 0.008350022875911727 60-61 0.017361745677839102 62-63 0.012477770033437903 64-65 0.0028988758663542604 66-67 0.004442842360390768 68-69 0.01115437018140661 70-71 0.0028988758663542604 72-73 0.0035920853126563657 74-75 0.0014494379331771302 76-77 4.096237637239715E-4 78-79 0.0035920853126563657 80-81 0.003087932988073016 82-83 0.002048118818619858 84-85 0.012320222432005607 86-87 0.00822398479476589 88-89 0.002268685460625073 90-91 0.0011028332100260772 92-93 0.002615290183776126 94-95 0.0 96-97 0.0015124569737500488 98-99 0.024703463904584128 100-101 0.015408155420078623 102-103 0.007152661105026272 104-105 0.014714945973776518 106-107 0.03330556294278753 108-109 0.0018275521766146423 110-111 0.002363214021484451 112-113 0.016700045751823454 114-115 0.0323287678139073 116-117 0.05113995142492353 118-119 0.36166627384798045 120-121 0.12011429133198305 122-123 0.020512697706485037 124-125 0.3232561686187865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1586822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.52406800214607 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20886062173922 53.68974181138195 2 9.73161266179984 12.55846474321037 3 3.0553873217083467 5.91438057956413 4 1.3489979796448561 3.4817134949344943 5 0.6837784806931893 2.2060084593325735 6 0.4303950578181409 1.6662503987067188 7 0.3063275434547162 1.3835849471361723 8 0.20422328746265822 1.054185383028991 9 0.13788897180585466 0.8007441654192272 >10 0.7893644449081262 9.489850364896444 >50 0.06939769094754554 3.1237677021711745 >100 0.0331444790362072 3.797782123666789 >500 4.143059875583909E-4 0.1678814044496593 >1k 2.0715299377919546E-4 0.6656444221013561 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4980 0.3138348220531351 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.30190405729187E-5 0.0 14-15 0.0 0.0 0.0 6.30190405729187E-5 0.0 16-17 0.0 0.0 0.0 6.30190405729187E-5 0.0 18-19 0.0 0.0 0.0 9.452856085937804E-5 0.0 20-21 0.0 0.0 0.0 2.520761622916748E-4 0.0 22-23 0.0 0.0 0.0 5.35661844869809E-4 0.0 24-25 0.0 0.0 0.0 0.001071323689739618 0.0 26-27 0.0 0.0 0.0 0.0020166092983333987 0.0 28-29 0.0 0.0 0.0 0.0038441614749480406 0.0 30-31 0.0 0.0 0.0 0.011406446343698286 0.0 32-33 0.0 0.0 0.0 0.028862720582396764 0.0 34-35 0.0 0.0 0.0 0.05205372751323085 0.0 36-37 0.0 0.0 0.0 0.08932949001211225 0.0 38-39 0.0 0.0 0.0 0.1501428641649788 0.0 40-41 0.0 0.0 0.0 0.22192155137753322 0.0 42-43 0.0 0.0 0.0 0.28780795829651973 0.0 44-45 0.0 0.0 0.0 0.36944912535873586 0.0 46-47 0.0 0.0 0.0 0.46397768621811397 0.0 48-49 0.0 0.0 0.0 0.566667212831685 0.0 50-51 0.0 0.0 0.0 0.6923586892543713 0.0 52-53 0.0 0.0 0.0 0.8066752288536458 0.0 54-55 0.0 0.0 0.0 0.9277032962739362 0.0 56-57 0.0 0.0 0.0 1.0691180233195658 0.0 58-59 0.0 0.0 0.0 1.2404983041576183 0.0 60-61 0.0 0.0 0.0 1.4364875203393954 0.0 62-63 0.0 0.0 0.0 1.6341467410963548 0.0 64-65 0.0 0.0 0.0 1.827457648053783 0.0 66-67 0.0 0.0 0.0 2.037720676925326 0.0 68-69 0.0 0.0 0.0 2.2887885345678343 0.0 70-71 0.0 0.0 0.0 2.5917525721221413 0.0 72-73 0.0 0.0 0.0 2.898655299712255 0.0 74-75 0.0 0.0 0.0 3.189362133875129 0.0 76-77 0.0 0.0 0.0 3.507671307808941 0.0 78-79 0.0 0.0 0.0 3.879893270952886 0.0 80-81 0.0 0.0 0.0 4.297110829065893 0.0 82-83 0.0 0.0 0.0 4.752896040009528 0.0 84-85 0.0 0.0 0.0 5.181992687270531 0.0 86-87 0.0 0.0 0.0 5.6161623672976555 0.0 88-89 0.0 0.0 0.0 6.102732379561161 0.0 90-91 0.0 0.0 0.0 6.650210294538391 0.0 92-93 0.0 0.0 0.0 7.238650585888021 0.0 94-95 0.0 0.0 0.0 7.82592502498705 0.0 96-97 0.0 0.0 0.0 8.38178447236048 0.0 98-99 0.0 0.0 0.0 8.970791935075264 0.0 100-101 0.0 0.0 0.0 9.653729277763983 0.0 102-103 0.0 0.0 0.0 10.383206181915805 0.0 104-105 0.0 0.0 0.0 11.106979862895777 0.0 106-107 0.0 0.0 0.0 11.812541041150173 0.0 108-109 0.0 0.0 0.0 12.51561296730194 0.0 110-111 0.0 0.0 0.0 13.293488494613763 0.0 112-113 0.0 0.0 0.0 14.139204019102332 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2740 0.0 51.673805 1 GTATCAA 4410 0.0 47.888783 1 GTACATG 11870 0.0 40.796013 1 TACATGG 11825 0.0 39.486088 2 TCAACGC 5210 0.0 38.472824 4 ATCAACG 5235 0.0 38.17548 3 CAACGCA 5335 0.0 37.79914 5 ACATGGG 11690 0.0 37.295063 3 AACGCAG 5500 0.0 36.44081 6 CATGGGG 3615 0.0 34.88104 4 TATCAAC 5985 0.0 34.287083 2 CATGGGA 8235 0.0 33.07987 4 ACGCAGA 6435 0.0 30.683884 7 CGCAGAG 6410 0.0 30.431469 8 ATGGGAG 3375 0.0 27.143356 5 ATGGGGA 2280 0.0 27.134083 5 GCAGAGT 7135 0.0 26.42241 9 ATGGGAT 3045 0.0 26.177858 5 GAGTACA 8155 0.0 24.292055 1 AGTACAT 8085 0.0 23.689257 2 >>END_MODULE