FastQCFastQC Report
Thu 26 May 2016
SRR1364186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586822
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109400.6894283038677307No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98930.6234473683878847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.40590564033016935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.15137173545615074No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.1246516622532332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.079.568131
GTATCAA97400.053.7245031
ATCAACG125600.041.5477033
TCAACGC127200.041.0250934
CAACGCA128450.040.5731475
TATCAAC128950.040.4696122
AACGCAG132150.039.8873526
TGGTATC19100.037.385112
GTACATG113500.035.6136441
TACATGG114800.035.298482
GTGGTAT21200.034.819961
ACATGGG115050.034.2897263
CGCAGAG155700.033.776818
ACGCAGA156400.033.7017147
GCAGAGT167950.031.0652289
CATGGGA81850.030.5328754
GAGTACT109250.029.9227312-13
CATGGGG35200.028.5680854
CAGAGTA166450.027.61020910-11
GTACTTT119100.027.39806214-15
Produced by FastQC (version 0.11.5)