##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1586822 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.375093740822855 33.0 31.0 34.0 31.0 34.0 2 32.33369905383213 34.0 31.0 34.0 30.0 34.0 3 32.48374360829381 34.0 31.0 34.0 30.0 34.0 4 36.06632312887016 37.0 35.0 37.0 35.0 37.0 5 36.02716498762936 37.0 35.0 37.0 35.0 37.0 6 36.09259261593298 37.0 35.0 37.0 35.0 37.0 7 36.03645903573306 37.0 35.0 37.0 35.0 37.0 8 36.05375713217992 37.0 35.0 37.0 35.0 37.0 9 37.76148994657246 39.0 38.0 39.0 35.0 39.0 10-11 37.74043780587867 39.0 38.0 39.0 35.0 39.0 12-13 37.69753475815183 39.0 38.0 39.0 35.0 39.0 14-15 39.06299099709987 41.0 38.0 41.0 36.0 41.0 16-17 39.10513970691105 41.0 38.5 41.0 36.0 41.0 18-19 39.189316445070716 41.0 39.0 41.0 36.0 41.0 20-21 39.222205137060115 41.0 39.0 41.0 36.0 41.0 22-23 39.170285325008095 41.0 39.0 41.0 35.5 41.0 24-25 39.11981022446122 41.0 39.0 41.0 35.0 41.0 26-27 39.012015210275635 41.0 39.0 41.0 35.0 41.0 28-29 38.905418188051335 41.0 38.0 41.0 35.0 41.0 30-31 38.707814739145284 40.0 38.0 41.0 35.0 41.0 32-33 38.471379272533405 40.0 38.0 41.0 34.0 41.0 34-35 38.33905976851846 40.0 38.0 41.0 34.0 41.0 36-37 38.20135686296257 40.0 38.0 41.0 34.0 41.0 38-39 38.072639212211584 40.0 37.5 41.0 34.0 41.0 40-41 37.90769223013041 40.0 37.0 41.0 33.0 41.0 42-43 37.71585061210394 40.0 37.0 41.0 33.0 41.0 44-45 37.44056768812129 40.0 37.0 41.0 33.0 41.0 46-47 37.045085397101886 40.0 37.0 41.0 31.5 41.0 48-49 36.6465479429955 40.0 36.5 41.0 30.5 41.0 50-51 36.42303011932025 40.0 36.0 41.0 30.0 41.0 52-53 36.2741769398206 40.0 36.0 41.0 30.0 41.0 54-55 36.08778142728043 40.0 35.0 41.0 29.5 41.0 56-57 35.9088357106216 39.0 35.0 41.0 29.0 41.0 58-59 35.69902200750935 39.0 35.0 41.0 28.5 41.0 60-61 35.475328360710904 39.0 35.0 41.0 28.0 41.0 62-63 35.23059486193158 38.5 35.0 41.0 28.0 41.0 64-65 34.94558809998853 38.0 35.0 40.0 28.0 41.0 66-67 34.650447561226144 37.0 35.0 40.0 27.5 41.0 68-69 34.315601875950804 37.0 34.0 39.5 26.5 41.0 70-71 33.97530882480833 36.0 34.0 39.0 26.0 41.0 72-73 33.63731754412278 36.0 34.0 39.0 26.0 41.0 74-75 33.50971816624675 35.5 34.0 38.5 26.0 40.0 76-77 33.22022949013815 35.0 34.0 37.0 26.0 39.5 78-79 32.91275265908841 35.0 34.0 37.0 26.0 39.0 80-81 32.608902573823656 35.0 34.0 36.5 26.0 39.0 82-83 32.33424826477072 35.0 34.0 36.0 26.0 37.5 84-85 32.097768369735235 35.0 34.0 36.0 25.0 37.0 86-87 31.906776248375685 35.0 34.0 35.0 25.0 37.0 88-89 31.736849816803648 35.0 33.0 35.0 25.0 36.0 90-91 31.600318435212017 35.0 33.0 35.0 24.5 36.0 92-93 31.47908744648108 35.0 33.0 35.0 24.0 36.0 94-95 31.38175516850661 35.0 33.0 35.0 24.0 36.0 96-97 31.3048549238667 35.0 33.0 35.0 24.0 35.0 98-99 31.232000501631564 35.0 33.0 35.0 23.0 35.0 100-101 31.163199779181284 35.0 33.0 35.0 23.0 35.0 102-103 30.261995044182648 34.0 31.5 35.0 19.5 35.0 104-105 30.936552429951185 35.0 33.0 35.0 20.0 35.0 106-107 31.033403872646083 35.0 33.0 35.0 20.0 35.0 108-109 31.022975796907275 35.0 33.0 35.0 20.0 35.0 110-111 30.96738386536108 35.0 33.0 35.0 19.5 35.0 112-113 31.065344128074855 35.0 33.0 35.0 20.0 35.0 114-115 31.035918017269736 35.0 33.0 35.0 19.0 35.0 116-117 30.98507110438348 35.0 33.0 35.0 19.0 35.0 118-119 30.894358346430792 35.0 33.0 35.0 17.5 35.0 120-121 30.78747773852392 35.0 33.0 35.0 7.5 35.0 122-123 30.656565134589762 35.0 33.0 35.0 2.0 35.0 124-125 29.995323356999084 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3079675787314997 1101 2 0.34624915488156915 1101 3 0.30153478828258784 1101 4 0.21122841664210057 1101 5 0.16358305596457967 1101 6 0.2525329858702321 1101 7 0.27545779195592246 1101 8 0.23355402237742595 1101 9 0.35909059260380616 1101 10-11 0.31400301629660277 1101 12-13 0.32128827072065747 1101 14-15 0.39134872402370746 1101 16-17 0.38732411574473957 1101 18-19 0.420741363747247 1101 20-21 0.31127482581799626 1101 22-23 0.2860676463626959 1101 24-25 0.2893286244562958 1101 26-27 0.2732959498304979 1101 28-29 0.32885577883801176 1101 30-31 0.36466002263624375 1101 32-33 0.45430967786362686 1101 34-35 0.363251681838932 1101 36-37 0.2921972229613914 1101 38-39 0.3766607889443776 1101 40-41 0.40825815666560317 1101 42-43 0.47728742089708476 1101 44-45 0.4657437898595873 1101 46-47 0.5959299264675266 1101 48-49 0.7832071962542599 1101 50-51 0.7565982377188973 1101 52-53 0.6861474824965512 1101 54-55 0.7173480587674774 1101 56-57 0.6686914960081225 1101 58-59 0.6729796299621924 1101 60-61 0.640976134407758 1101 62-63 0.6611342311339499 1101 64-65 0.6716004771556996 1101 66-67 0.6679166909821461 1101 68-69 0.6552133204121375 1101 70-71 0.6748716774110264 1101 72-73 0.6466832252854431 1101 74-75 0.6475228845372172 1101 76-77 0.6407983575155214 1101 78-79 0.6456892182655594 1101 80-81 0.6555871454297986 1101 82-83 0.685114067420102 1101 84-85 0.6490326813465259 1101 86-87 0.4974310349609645 1101 88-89 0.6504926936160125 1101 90-91 0.69896842069058 1101 92-93 0.5571084074398662 1101 94-95 0.6163542501209776 1101 96-97 0.6478974887860716 1101 98-99 0.6733007464730179 1101 100-101 0.6127085743559668 1101 102-103 0.6434166330541018 1101 104-105 0.6552730056521803 1101 106-107 0.7030976348615638 1101 108-109 0.7030817302708243 1101 110-111 0.6499330344423555 1101 112-113 0.7604084522724612 1101 114-115 0.7303545907010829 1101 116-117 0.678943358092436 1101 118-119 0.759987692093226 1101 120-121 0.8233958915300299 1101 122-123 0.7951290180064348 1101 124-125 0.6899609220719682 1102 1 0.24806380766567315 1102 2 0.31742245932942836 1102 3 0.2522699403931554 1102 4 0.17827462500377322 1102 5 0.19106624109191728 1102 6 0.1905005904751107 1102 7 0.19762235518908255 1102 8 0.16086590992560446 1102 9 0.2879097309098029 1102 10-11 0.269033203296452 1102 12-13 0.3004638591362436 1102 14-15 0.3466812008691491 1102 16-17 0.359887113788254 1102 18-19 0.26726629006409297 1102 20-21 0.24333728225887086 1102 22-23 0.2405856200130998 1102 24-25 0.2359534743609899 1102 26-27 0.2715346916329935 1102 28-29 0.23969286980206306 1102 30-31 0.22150268916855964 1102 32-33 0.18914480394368383 1102 34-35 0.2623267588808602 1102 36-37 0.2261091054714086 1102 38-39 0.2672528571947055 1102 40-41 0.2751624567386557 1102 42-43 0.21764353512905643 1102 44-45 0.24507430182102752 1102 46-47 0.17997909894907593 1102 48-49 0.11684072019813385 1102 50-51 0.2556871697120826 1102 52-53 0.23368465994908405 1102 54-55 0.22694786059968663 1102 56-57 0.26734294741922326 1102 58-59 0.3216663485219513 1102 60-61 0.23300659850664118 1102 62-63 0.12928673636104548 1102 64-65 0.2818627975661414 1102 66-67 0.2168141727397952 1102 68-69 0.1408503542769992 1102 70-71 0.12477343321121026 1102 72-73 0.07716915621220721 1102 74-75 0.06181741561009346 1102 76-77 0.14557411722868352 1102 78-79 0.10669592742074485 1102 80-81 0.16972549341578969 1102 82-83 0.224655258610035 1102 84-85 0.2273257513518132 1102 86-87 0.18889386590536716 1102 88-89 0.12159882254262655 1102 90-91 0.1815174798134329 1102 92-93 0.17133618283850893 1102 94-95 0.16974199562652004 1102 96-97 0.17818525192518564 1102 98-99 0.13019085571720623 1102 100-101 0.05975117203337277 1102 102-103 0.07563023441776195 1102 104-105 0.1284342856817382 1102 106-107 0.02812009667648141 1102 108-109 0.040313343071119334 1102 110-111 0.11396054281830459 1102 112-113 0.05433494545834705 1102 114-115 0.06989116357392788 1102 116-117 0.10733005398045137 1102 118-119 0.09094888983276661 1102 120-121 0.18318378482171482 1102 122-123 0.17045334981992255 1102 124-125 0.1825674162654849 1103 1 0.1443542026478184 1103 2 0.24248548388816005 1103 3 0.1861788380107683 1103 4 0.11842156259083225 1103 5 0.08431724531563844 1103 6 0.11930108546656015 1103 7 0.09765022060393846 1103 8 0.15755999716187574 1103 9 0.2816722686614739 1103 10-11 0.21934167748300837 1103 12-13 0.2710037414390456 1103 14-15 0.3002878848713024 1103 16-17 0.34919734752073595 1103 18-19 0.25303470824636776 1103 20-21 0.27809539139308725 1103 22-23 0.32793079316284945 1103 24-25 0.2410113263900442 1103 26-27 0.2816742748303227 1103 28-29 0.27902845586257996 1103 30-31 0.34636584148069005 1103 32-33 0.40509031097773374 1103 34-35 0.39620678970035783 1103 36-37 0.42243356674165966 1103 38-39 0.36526873181535535 1103 40-41 0.39306965431561736 1103 42-43 0.4323329801836451 1103 44-45 0.4186652448486754 1103 46-47 0.4067306867674034 1103 48-49 0.24068457989895364 1103 50-51 0.2179114306479093 1103 52-53 0.33468889329680707 1103 54-55 0.3116592032100698 1103 56-57 0.4430611558598514 1103 58-59 0.4877767660467782 1103 60-61 0.46205664976013594 1103 62-63 0.3901793308815158 1103 64-65 0.2929260605311086 1103 66-67 0.3099017011468277 1103 68-69 0.23868049156086357 1103 70-71 0.2752711303185862 1103 72-73 0.18514648335468564 1103 74-75 0.23470806436586855 1103 76-77 0.23384499216997057 1103 78-79 0.26530299945891045 1103 80-81 0.25934255482966506 1103 82-83 0.2579576068345091 1103 84-85 0.23705033821863708 1103 86-87 0.28710038849223807 1103 88-89 0.2767916591089836 1103 90-91 0.2891058080753375 1103 92-93 0.19842908828848138 1103 94-95 0.17100576055410954 1103 96-97 0.2284812665931426 1103 98-99 0.19800096726167382 1103 100-101 0.23092993685408558 1103 102-103 0.24241802994377437 1103 104-105 0.28320028263483366 1103 106-107 0.29441380585205934 1103 108-109 0.2761082882810122 1103 110-111 0.2656927336503685 1103 112-113 0.2844032301677828 1103 114-115 0.4594512039142664 1103 116-117 0.4702152663805812 1103 118-119 0.3482495033533297 1103 120-121 0.3087287443302067 1103 122-123 0.2810260890479732 1103 124-125 0.33115680426048044 1104 1 0.21709134852849843 1104 2 0.2892746747368875 1104 3 0.24503264141811343 1104 4 0.15420196828810617 1104 5 0.15808481941914465 1104 6 0.2041228666205157 1104 7 0.18099921985754008 1104 8 0.16005134197267523 1104 9 0.2673436061825214 1104 10-11 0.2887430020226631 1104 12-13 0.19743416973603445 1104 14-15 0.3571802038242424 1104 16-17 0.34843110789407916 1104 18-19 0.2989758073524982 1104 20-21 0.340433610639586 1104 22-23 0.4022304523688405 1104 24-25 0.38211093309202226 1104 26-27 0.41352292387180967 1104 28-29 0.37041129127919703 1104 30-31 0.2934895599805998 1104 32-33 0.409982527592355 1104 34-35 0.4259877041229032 1104 36-37 0.38899609675256386 1104 38-39 0.348767141307313 1104 40-41 0.2930740852682163 1104 42-43 0.3030241568777967 1104 44-45 0.36313660254075586 1104 46-47 0.2772804993881621 1104 48-49 0.3157560405967672 1104 50-51 0.3504776529956999 1104 52-53 0.32330486764103483 1104 54-55 0.2997661741923352 1104 56-57 0.32743878499987034 1104 58-59 0.1733203104454759 1104 60-61 0.36805584440205763 1104 62-63 0.2836273424590985 1104 64-65 0.34269483525591937 1104 66-67 0.34776826977634556 1104 68-69 0.40032599903842225 1104 70-71 0.48745294991615395 1104 72-73 0.4170570730347549 1104 74-75 0.42366520352039316 1104 76-77 0.37707701798295545 1104 78-79 0.3681004507898109 1104 80-81 0.33872946672267545 1104 82-83 0.3393903581371944 1104 84-85 0.3635805463769941 1104 86-87 0.4294185071579726 1104 88-89 0.3915391485757418 1104 90-91 0.3797346399687065 1104 92-93 0.3525615355796141 1104 94-95 0.4045560807877706 1104 96-97 0.4080907643307583 1104 98-99 0.4583573456272063 1104 100-101 0.3672482861518276 1104 102-103 0.3450400044281636 1104 104-105 0.3178977004833037 1104 106-107 0.4365238180622093 1104 108-109 0.42929299374502605 1104 110-111 0.4157313700738392 1104 112-113 0.37059953360386544 1104 114-115 0.2955885515794172 1104 116-117 0.3371669230192076 1104 118-119 0.34320554051696917 1104 120-121 0.35990355857317624 1104 122-123 0.37599790336918204 1104 124-125 0.44668907290449766 1105 1 0.1388655784477706 1105 2 0.27050402516744754 1105 3 0.2181862007455777 1105 4 0.13888764383363394 1105 5 0.08579004940679624 1105 6 0.11767400335612876 1105 7 0.1253343091771555 1105 8 0.146887264185537 1105 9 0.18157742441248104 1105 10-11 0.2099802158383639 1105 12-13 0.23134529093400147 1105 14-15 0.24848490745245755 1105 16-17 0.264898643970362 1105 18-19 0.29014059218962274 1105 20-21 0.28574253038068065 1105 22-23 0.24254644988397445 1105 24-25 0.2015504068489733 1105 26-27 0.24401951010062817 1105 28-29 0.13801825441221638 1105 30-31 0.21637219259248752 1105 32-33 0.29143654097393323 1105 34-35 0.24564558503881528 1105 36-37 0.29173235110432927 1105 38-39 0.27567948602182213 1105 40-41 0.29737439674846655 1105 42-43 0.11826333908574327 1105 44-45 0.16682584679742973 1105 46-47 0.11968091227043942 1105 48-49 0.17186409412889248 1105 50-51 -0.0038640485506462596 1105 52-53 -0.032249621035255416 1105 54-55 0.05461659529442642 1105 56-57 0.02716985868291033 1105 58-59 -0.10609755173214808 1105 60-61 -0.01664536151097451 1105 62-63 0.02824208750647017 1105 64-65 -0.008179084346970455 1105 66-67 -0.027633810347253984 1105 68-69 -0.11821351356263676 1105 70-71 -0.076062070531556 1105 72-73 0.06944871325978852 1105 74-75 -0.031916126868345884 1105 76-77 0.039912012010859144 1105 78-79 0.10913957422240372 1105 80-81 0.07321292015266323 1105 82-83 0.07831233240698054 1105 84-85 0.011464742936894368 1105 86-87 0.10207182134567105 1105 88-89 0.10879189566861314 1105 90-91 0.04398227913462094 1105 92-93 0.04444722771026122 1105 94-95 0.10853418260587588 1105 96-97 0.1395572529255773 1105 98-99 0.09372013792181733 1105 100-101 0.07441956973852015 1105 102-103 0.11617948967831282 1105 104-105 0.15086968908321552 1105 106-107 0.2586460082295581 1105 108-109 0.1493733952213958 1105 110-111 0.16473971791915432 1105 112-113 0.15441499937668013 1105 114-115 0.1335304396356527 1105 116-117 0.038522502240081025 1105 118-119 0.14928908007321695 1105 120-121 0.12262463899211085 1105 122-123 0.07275838509426791 1105 124-125 0.13556652921215573 1106 1 0.0447001021264839 1106 2 0.177191875846745 1106 3 0.10388132109746806 1106 4 0.06481283461380372 1106 5 0.0641407092066757 1106 6 0.008505390194152085 1106 7 0.08360050466790625 1106 8 0.038170745972365694 1106 9 0.24314598653432995 1106 10-11 0.19711323206131937 1106 12-13 0.14513906049495517 1106 14-15 0.11894233680265387 1106 16-17 0.1127041846589023 1106 18-19 0.1303430158864174 1106 20-21 0.10509515380739032 1106 22-23 0.14241557096602264 1106 24-25 0.08978911510860854 1106 26-27 0.07205675166571268 1106 28-29 0.17622648037816901 1106 30-31 0.18032767781033954 1106 32-33 0.1396759226795936 1106 34-35 0.12109971804794384 1106 36-37 0.080078722116383 1106 38-39 0.08201129642196037 1106 40-41 0.10246910452605107 1106 42-43 0.004607271514302624 1106 44-45 0.09176638860247976 1106 46-47 0.07701323307177432 1106 48-49 0.17503531600809197 1106 50-51 0.18286383672251105 1106 52-53 0.0993179840843581 1106 54-55 0.22099568869430897 1106 56-57 0.14311635162413694 1106 58-59 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100-101 -0.6426350306203616 2216 102-103 -0.607629610538595 2216 104-105 -0.6430174023890558 2216 106-107 -0.6771110589363545 2216 108-109 -0.7482799451654571 2216 110-111 -0.6403267681680163 2216 112-113 -0.5873567140509621 2216 114-115 -0.7071760000773857 2216 116-117 -0.597399160112392 2216 118-119 -0.6306323101139881 2216 120-121 -0.6775067897341636 2216 122-123 -0.6051374062409778 2216 124-125 -0.6817347282856439 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55.0 3 11.0 4 37.0 5 142.0 6 501.0 7 1024.0 8 1637.0 9 2575.0 10 4653.0 11 6914.0 12 8512.0 13 12709.0 14 20564.0 15 19619.0 16 7736.0 17 5872.0 18 5464.0 19 4605.0 20 4368.0 21 4408.0 22 4470.0 23 4869.0 24 5902.0 25 7485.0 26 9380.0 27 12153.0 28 15612.0 29 19884.0 30 24956.0 31 31772.0 32 40742.0 33 53579.0 34 74640.0 35 119269.0 36 234153.0 37 526831.0 38 287830.0 39 1889.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73089370867378 19.31867978050566 13.485043275290368 22.46538323553019 2 15.92925652293655 21.23772724952371 39.63725786152424 23.1957583660155 3 18.550996808071517 27.116771646348187 30.835919751199732 23.496311794380567 4 11.931173892571719 17.33492342534562 37.870558358036696 32.86334432404596 5 12.04583897371519 39.63713753456703 34.167920785090736 14.14910270662704 6 29.998008535567806 38.023484155253556 17.587025735266316 14.391481573912321 7 26.634543700321206 34.618945197908516 21.60340908446395 17.143102017306322 8 26.621952556745494 34.86207022589805 19.870092549762987 18.645884667593467 9 27.580048411257472 14.581165739550963 20.421143909741552 37.41764193945001 10-11 24.80432587902109 27.06365301212108 27.636307033807196 20.495714075050635 12-13 25.83475021142888 25.15537344453253 28.363452233457814 20.64642411058077 14-15 22.77328522039649 25.526429555425878 27.604104304074433 24.0961809201032 16-17 21.001977537493175 29.074180973039194 27.9813992999845 21.94244218948313 18-19 20.838055877744157 26.191711828213133 32.13211339652326 20.838118897519447 20-21 22.160235993703136 25.32385484950423 32.18376100154901 20.332148155243626 22-23 21.49863059624835 25.60586505606804 32.00674051657968 20.88876383110393 24-25 21.02642892523547 25.783862336166248 32.35375486349446 20.835953875103826 26-27 21.03483360226819 25.988245010210704 32.0426350763526 20.934286311168503 28-29 20.758939262172145 25.92584540149481 32.34213710439747 20.973078231935567 30-31 20.943857158206814 25.642598696700347 32.47885627751378 20.934687867579058 32-33 20.977526149750886 25.759726043626824 32.10010322518846 21.162644581433835 34-35 21.346943765589334 25.84366740566995 31.82426888460079 20.98511994413992 36-37 21.51447988495244 25.548076595862675 31.670628463684018 21.266815055500867 38-39 21.224529279276442 25.855546494817943 31.658875412617167 21.261048813288447 40-41 21.344359984925845 25.706884578106433 31.779966499078032 21.168788937889694 42-43 21.413439587085065 25.905559573157376 31.29466037084828 21.386340468909275 44-45 21.450515558770928 26.031716222739536 30.901039940207536 21.616728278282 46-47 21.718724595449267 26.256505140463137 30.225129220542694 21.799641043544895 48-49 21.928699585764623 26.557479018817364 29.60717907156573 21.90664232385228 50-51 21.875207765964856 26.666042777968414 29.30080667548303 22.1579427805837 52-53 22.1215239941539 26.82159628259676 29.11922803253799 21.937651690711345 54-55 21.76075829551141 26.818950077576435 29.153584964161073 22.266706662751083 56-57 21.84996174744237 27.07584719647194 28.93141133662125 22.14277971946444 58-59 21.99897530135971 26.55154246764574 29.11048469632117 22.338997534673382 60-61 21.96292337766933 26.711219027717036 29.337316976951417 21.98854061766222 62-63 21.83715364703547 26.537013900595696 29.526579394726188 22.09925305764265 64-65 21.584935172312964 26.40315044787632 29.57830178810226 22.43361259170846 66-67 21.75682816551513 26.901896058807978 29.050886647678663 22.29038912799823 68-69 21.884015270519168 26.563266618033772 29.27682463954674 22.275893471900325 70-71 21.608461113278505 26.67067722057899 29.36295849045188 22.357903175690623 72-73 21.85487723260706 26.71670168424688 29.032588406261066 22.395832676884993 74-75 21.636862861745048 26.93301454101342 28.92246263285989 22.507659964381638 76-77 21.74798195369695 26.778871028423744 28.921979799685783 22.551167218193516 78-79 21.816474562171905 26.946452316282354 28.771592062061618 22.465481059484123 80-81 21.65408070601536 27.18072800901929 28.64075406110708 22.524437223858268 82-83 21.68702678056861 27.067214056434842 28.594529820485022 22.651229342511535 84-85 21.655778404405595 26.793191708724567 28.58629676509429 22.96473312177555 86-87 21.618839416141192 26.58666189402466 28.64615564946793 23.148343040366214 88-89 21.66468576815799 26.580958670852812 28.657247000608766 23.097108560380434 90-91 21.518642328880254 26.90584951967963 28.67396161659384 22.90154653484627 92-93 21.51751667973862 26.7240088975412 28.55358865289348 23.204885769826696 94-95 21.39169816267507 26.506498671148897 28.57269354368066 23.529109622495366 96-97 21.657681639191672 26.508084003941253 28.490619366633553 23.34361499023353 98-99 21.306773239819215 26.524147554487755 28.31998542833571 23.84909377735732 100-101 21.18698577705868 26.60964169886165 28.487450651773194 23.71592187230647 102-103 21.668813614559866 26.40408658687606 28.344597586740218 23.582502211823858 104-105 21.367006929950804 26.386848881316045 28.37630663338509 23.869837555348063 106-107 21.287953075252265 26.47680791182162 28.36612852234262 23.8691104905835 108-109 21.41979569516281 26.6155431791749 27.90078513321419 24.063875992448096 110-111 21.230574594251472 26.665622366267037 28.194787028906532 23.90901601057496 112-113 21.23797318295131 26.76911946036921 27.988652022780407 24.00425533389907 114-115 21.067573899351746 26.89904578607244 27.82250606329013 24.210874251285684 116-117 20.951928112421704 26.90685910294295 27.926482738019843 24.214730046615507 118-119 20.964462654180352 26.986154256294682 27.702705683567814 24.346677405957156 120-121 20.805263602344812 27.097998389233325 27.806994105198946 24.28974390322292 122-123 20.857421132185102 27.182317552155528 27.469496315421477 24.4907650002379 124-125 20.735125415374295 27.256403329110107 27.426383270089993 24.58208798542561 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 360.0 1 312.5 2 554.5 3 868.5 4 591.0 5 425.5 6 1049.0 7 2507.0 8 4037.5 9 4468.0 10 3457.0 11 2284.0 12 2101.0 13 2300.5 14 2488.0 15 2669.5 16 2918.0 17 3102.5 18 3278.0 19 3563.5 20 3834.0 21 4308.5 22 5021.0 23 5830.5 24 6801.0 25 8135.0 26 10651.5 27 13566.0 28 15782.5 29 18206.0 30 21029.0 31 24339.0 32 28461.5 33 32024.5 34 35600.5 35 38492.0 36 41595.0 37 45874.5 38 47716.0 39 47211.0 40 46992.0 41 48818.0 42 51064.0 43 52498.0 44 53929.0 45 55656.0 46 55701.0 47 54580.0 48 55535.0 49 56280.5 50 54810.0 51 52072.5 52 49900.0 53 49575.5 54 48257.0 55 44294.5 56 39625.0 57 35181.5 58 32382.5 59 29450.0 60 25825.5 61 22024.5 62 17912.5 63 13736.0 64 10268.5 65 8294.5 66 6123.5 67 4787.0 68 3621.0 69 2245.0 70 1623.0 71 1294.0 72 1136.0 73 1081.5 74 859.5 75 715.5 76 492.0 77 227.5 78 142.5 79 66.5 80 32.0 81 24.5 82 19.0 83 8.0 84 6.0 85 5.5 86 4.0 87 2.5 88 1.5 89 2.0 90 2.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051234479985782906 2 0.004726428042968902 3 4.4113328401043086E-4 4 0.02085930242963609 5 0.003150952028645935 6 0.003150952028645935 7 1.890571217187561E-4 8 0.0 9 6.30190405729187E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.001165852250598996 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.096237637239715E-4 28-29 1.260380811458374E-4 30-31 2.2056664200521543E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.003434537711224069 44-45 0.0 46-47 0.0 48-49 0.002709818744635504 50-51 3.150952028645935E-5 52-53 0.007751341990469 54-55 0.0 56-57 0.0 58-59 8.822665680208617E-4 60-61 0.0 62-63 0.0012288712911719146 64-65 0.0 66-67 0.0019220807374740203 68-69 0.006585489739870004 70-71 0.011028332100260774 72-73 0.0 74-75 0.0 76-77 9.452856085937804E-4 78-79 7.562284868750244E-4 80-81 6.30190405729187E-5 82-83 0.001165852250598996 84-85 0.001354909372317752 86-87 0.0 88-89 0.0 90-91 0.0010398141694531586 92-93 0.005766242212422061 94-95 0.007121151584739813 96-97 9.767951288802397E-4 98-99 0.011406446343698286 100-101 0.016731555272109917 102-103 0.029083287224401982 104-105 0.009988517930807614 106-107 0.0338727343079438 108-109 0.029429891947553036 110-111 0.0272872445680738 112-113 0.00677454686158876 114-115 0.001165852250598996 116-117 6.932094463021057E-4 118-119 0.007877380071614837 120-121 0.0 122-123 3.4660472315105285E-4 124-125 0.04742182803112133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1586822.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.167036131675175 #Duplication Level Percentage of deduplicated Percentage of total 1 80.27273336185672 45.08681515123734 2 11.099312489875878 12.468309713112241 3 3.6574591044930522 6.162859130165569 4 1.6336217623371077 3.6702277020271676 5 0.8868270296951053 2.4905222909715574 6 0.5216729083471854 1.7580492655231459 7 0.3592467616236658 1.4124478088212615 8 0.24108176533102374 1.0832678579228519 9 0.1752148697220281 0.885716992663552 >10 1.0038702555064198 10.550374612960915 >50 0.09233260977941238 3.6409564051870373 >100 0.05207872147918674 5.311816266523107 >500 0.002729015971892239 1.0108864717079684 >1k 0.001137089988288433 0.9839346084870458 >5k 4.5483599531537326E-4 2.086406321566585 >10k+ 2.2741799765768663E-4 1.3974094011227158 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10940 0.6894283038677307 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9893 0.6234473683878847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6441 0.40590564033016935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2402 0.15137173545615074 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.1246516622532332 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.30190405729187E-5 0.0 14-15 0.0 0.0 0.0 6.30190405729187E-5 0.0 16-17 0.0 0.0 0.0 6.30190405729187E-5 0.0 18-19 0.0 0.0 0.0 1.260380811458374E-4 0.0 20-21 0.0 0.0 0.0 2.8358568257813414E-4 0.0 22-23 0.0 0.0 0.0 5.041523245833496E-4 0.0 24-25 0.0 0.0 0.0 0.0010398141694531586 0.0 26-27 0.0 0.0 0.0 0.0020166092983333987 0.0 28-29 0.0 0.0 0.0 0.0038441614749480406 0.0 30-31 0.0 0.0 0.0 0.011469465384271203 0.0 32-33 0.0 0.0 0.0 0.02917781578526136 0.0 34-35 0.0 0.0 0.0 0.052746936959532954 0.0 36-37 0.0 0.0 0.0 0.0904638327424248 0.0 38-39 0.0 0.0 0.0 0.15266362578789555 0.0 40-41 0.0 0.0 0.0 0.2254191081293302 0.0 42-43 0.0 0.0 0.0 0.2920932530554782 0.0 44-45 0.0 0.0 0.0 0.37398649627998604 0.0 46-47 0.0 0.0 0.0 0.46961789034939017 0.0 48-49 0.0 0.0 0.0 0.5723389264832477 0.0 50-51 0.0 0.0 0.0 0.6980619124262204 0.0 52-53 0.0 0.0 0.0 0.8131976995529429 0.0 54-55 0.0 0.0 0.0 0.9347929383383895 0.0 56-57 0.0 0.0 0.0 1.0767433272288889 0.0 58-59 0.0 0.0 0.0 1.2486277603915248 0.0 60-61 0.0 0.0 0.0 1.4458458478644738 0.0 62-63 0.0 0.0 0.0 1.6437886543040114 0.0 64-65 0.0 0.0 0.0 1.8374776755048772 0.0 66-67 0.0 0.0 0.0 2.048496932863295 0.0 68-69 0.0 0.0 0.0 2.300825171317262 0.0 70-71 0.0 0.0 0.0 2.6046399659193025 0.0 72-73 0.0 0.0 0.0 2.9127400552803024 0.0 74-75 0.0 0.0 0.0 3.204738779774921 0.0 76-77 0.0 0.0 0.0 3.525001543966494 0.0 78-79 0.0 0.0 0.0 3.898578416482756 0.0 80-81 0.0 0.0 0.0 4.317056355407223 0.0 82-83 0.0 0.0 0.0 4.773597794837732 0.0 84-85 0.0 0.0 0.0 5.204112370511627 0.0 86-87 0.0 0.0 0.0 5.639227336147344 0.0 88-89 0.0 0.0 0.0 6.127498862506318 0.0 90-91 0.0 0.0 0.0 6.676867348700736 0.0 92-93 0.0 0.0 0.0 7.268080477835573 0.0 94-95 0.0 0.0 0.0 7.859388135531269 0.0 96-97 0.0 0.0 0.0 8.419249291981078 0.0 98-99 0.0 0.0 0.0 9.011533744805655 0.0 100-101 0.0 0.0 0.0 9.69815770136789 0.0 102-103 0.0 0.0 0.0 10.432424052603254 0.0 104-105 0.0 0.0 0.0 11.16237359955937 0.0 106-107 0.0 0.0 0.0 11.874929891317363 0.0 108-109 0.0 0.0 0.0 12.588904111488244 0.0 110-111 0.0 0.0 0.0 13.377020232893166 0.0 112-113 0.0 0.0 0.0 14.23461484652973 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5125 0.0 79.56813 1 GTATCAA 9740 0.0 53.724503 1 ATCAACG 12560 0.0 41.547703 3 TCAACGC 12720 0.0 41.025093 4 CAACGCA 12845 0.0 40.573147 5 TATCAAC 12895 0.0 40.469612 2 AACGCAG 13215 0.0 39.887352 6 TGGTATC 1910 0.0 37.38511 2 GTACATG 11350 0.0 35.613644 1 TACATGG 11480 0.0 35.29848 2 GTGGTAT 2120 0.0 34.81996 1 ACATGGG 11505 0.0 34.289726 3 CGCAGAG 15570 0.0 33.77681 8 ACGCAGA 15640 0.0 33.701714 7 GCAGAGT 16795 0.0 31.065228 9 CATGGGA 8185 0.0 30.532875 4 GAGTACT 10925 0.0 29.92273 12-13 CATGGGG 3520 0.0 28.568085 4 CAGAGTA 16645 0.0 27.610209 10-11 GTACTTT 11910 0.0 27.398062 14-15 >>END_MODULE