FastQCFastQC Report
Thu 26 May 2016
SRR1364185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917413
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36550.047.8551331
GGTATCA24600.044.2572631
CAACGCA44450.038.2791985
ATCAACG45200.037.376893
AACGCAG46550.036.6657756
GTACATG95850.036.0622331
TCAACGC46900.036.0230184
TACATGG94950.035.7110942
TATCAAC49000.035.2066882
ACATGGG99900.032.8102383
ACGCAGA55850.030.2400477
CGCAGAG56300.029.787098
CATGGGA56050.027.8073944
TGGTATC13350.025.844612
CATGGGG48450.025.7845844
GTGGTAT15250.025.747951
CATGGGT13950.023.8807744
GCAGAGT70050.023.4333069
TAGACCG2102.796336E-622.6623694
GTATAGG6150.022.2495541
Produced by FastQC (version 0.11.5)