##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1917413 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16180342993398 33.0 31.0 34.0 28.0 34.0 2 31.19383043715673 33.0 31.0 34.0 28.0 34.0 3 31.31702663953984 34.0 31.0 34.0 28.0 34.0 4 34.645364874442805 37.0 35.0 37.0 32.0 37.0 5 34.79794493935318 37.0 35.0 37.0 32.0 37.0 6 34.82425069612024 37.0 35.0 37.0 32.0 37.0 7 34.72434629367799 37.0 35.0 37.0 32.0 37.0 8 34.70871794443868 37.0 35.0 37.0 32.0 37.0 9 36.197344025517715 39.0 37.0 39.0 32.0 39.0 10-11 36.18826330060347 39.0 37.0 39.0 32.0 39.0 12-13 36.16078982462307 39.0 37.0 39.0 32.0 39.0 14-15 37.51275755405852 40.0 38.0 41.0 31.5 41.0 16-17 37.56191415203715 40.0 38.0 41.0 32.0 41.0 18-19 37.47239692231147 40.0 38.0 41.0 31.0 41.0 20-21 37.46165354047355 40.0 38.0 41.0 31.0 41.0 22-23 37.53266275966628 40.0 38.0 41.0 31.0 41.0 24-25 37.40126748905948 40.0 38.0 41.0 30.0 41.0 26-27 37.445803799181505 40.0 38.0 41.0 30.5 41.0 28-29 37.54138805776324 40.0 38.0 41.0 31.0 41.0 30-31 37.43986871894579 40.0 38.0 41.0 30.0 41.0 32-33 37.25129640823339 40.0 37.5 41.0 30.0 41.0 34-35 37.21308606961567 40.0 37.0 41.0 30.0 41.0 36-37 37.23828538765514 40.0 37.0 41.0 30.0 41.0 38-39 37.0697742218291 40.0 37.0 41.0 30.0 41.0 40-41 37.06524885353338 40.0 37.0 41.0 30.0 41.0 42-43 36.989811271750014 40.0 37.0 41.0 30.0 41.0 44-45 36.97944600354749 40.0 37.0 41.0 30.0 41.0 46-47 36.900620262822876 40.0 36.5 41.0 30.0 41.0 48-49 36.806704919597394 40.0 36.0 41.0 30.0 41.0 50-51 36.68699023110827 40.0 36.0 41.0 30.0 41.0 52-53 36.53326226535441 40.0 35.5 41.0 30.0 41.0 54-55 36.343957457261425 39.5 35.0 41.0 30.0 41.0 56-57 36.140500507715345 39.0 35.0 41.0 30.0 41.0 58-59 35.89753981015045 39.0 35.0 41.0 29.0 41.0 60-61 35.64894130789767 38.5 35.0 40.5 29.0 41.0 62-63 35.341503630151664 38.0 35.0 40.0 29.0 41.0 64-65 35.00810858171923 37.0 34.0 40.0 28.0 41.0 66-67 34.68317649875118 37.0 34.0 40.0 28.0 41.0 68-69 34.42933108307913 36.0 34.0 39.0 28.0 41.0 70-71 34.23433918514165 36.0 34.0 39.0 28.0 41.0 72-73 33.91941120666231 35.5 34.0 39.0 28.0 40.5 74-75 33.588356551249 35.0 34.0 37.5 28.0 40.0 76-77 33.27248276714511 35.0 34.0 37.0 27.5 39.0 78-79 32.945217592662615 35.0 34.0 36.5 27.0 39.0 80-81 32.670128188345444 35.0 33.0 36.0 27.0 38.0 82-83 32.417758719691584 35.0 33.0 36.0 26.5 37.0 84-85 32.185881967004505 35.0 33.0 35.0 26.0 37.0 86-87 32.00601487525118 35.0 33.0 35.0 26.0 36.5 88-89 31.84944088727885 35.0 33.0 35.0 26.0 36.0 90-91 31.712347053034478 35.0 33.0 35.0 25.5 36.0 92-93 31.593764880075394 35.0 33.0 35.0 25.0 36.0 94-95 31.471625309727223 35.0 33.0 35.0 25.0 35.0 96-97 31.387904431648266 35.0 33.0 35.0 25.0 35.0 98-99 31.318808467450673 35.0 33.0 35.0 25.0 35.0 100-101 31.225862920507996 35.0 33.0 35.0 24.0 35.0 102-103 30.266759952081266 34.0 30.5 35.0 21.5 35.0 104-105 30.94755876798582 35.0 32.0 35.0 23.0 35.0 106-107 31.045451866655746 35.0 33.0 35.0 23.0 35.0 108-109 31.02228758227883 35.0 33.0 35.0 23.0 35.0 110-111 30.954335607404353 35.0 33.0 35.0 23.0 35.0 112-113 31.101324545103218 35.0 33.0 35.0 23.0 35.0 114-115 31.097536368012527 35.0 33.0 35.0 23.0 35.0 116-117 31.014890897266262 35.0 33.0 35.0 21.5 35.0 118-119 30.7808398607916 35.0 33.0 35.0 18.5 35.0 120-121 30.725490022233082 35.0 33.0 35.0 18.0 35.0 122-123 30.604754948464418 35.0 33.0 35.0 8.5 35.0 124-125 29.756575396119665 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23654109480859375 1101 2 0.31917260085783994 1101 3 0.37287760669039827 1101 4 0.38274191782912226 1101 5 0.2884905604621011 1101 6 0.26829125679369525 1101 7 0.23152848024697903 1101 8 0.21605102753076721 1101 9 0.2693571409820308 1101 10-11 0.3072229948114895 1101 12-13 0.3971946333869383 1101 14-15 0.4792012349993158 1101 16-17 0.4327595747229154 1101 18-19 0.5598428995412377 1101 20-21 0.6424841243887229 1101 22-23 0.5625810080628497 1101 24-25 0.742654406156305 1101 26-27 0.7663759520881399 1101 28-29 0.6532996813055618 1101 30-31 0.7424505733518174 1101 32-33 0.8686939289270654 1101 34-35 0.7666222868567232 1101 36-37 0.7383287480100336 1101 38-39 0.7969915238237846 1101 40-41 0.6162055550568937 1101 42-43 0.5997301281980398 1101 44-45 0.4990268823955972 1101 46-47 0.45589906132099856 1101 48-49 0.47633316380375135 1101 50-51 0.45264412484105776 1101 52-53 0.41813116584010146 1101 54-55 0.3595560183192532 1101 56-57 0.329299210663919 1101 58-59 0.30541332980020997 1101 60-61 0.2483422049144579 1101 62-63 0.2508945619652678 1101 64-65 0.23148297414988406 1101 66-67 0.17802782942445816 1101 68-69 0.13485438941344796 1101 70-71 0.1543673020866123 1101 72-73 0.1696305911953786 1101 74-75 0.14995273422434963 1101 76-77 0.16831193235903896 1101 78-79 0.1734529469741446 1101 80-81 0.15616975561339785 1101 82-83 0.18649921023163785 1101 84-85 0.16159321655823078 1101 86-87 0.20638653692246578 1101 88-89 0.25494356888340874 1101 90-91 0.2854235717665006 1101 92-93 0.2058564831288443 1101 94-95 0.2816877387218071 1101 96-97 0.31852928368160605 1101 98-99 0.28659019804761954 1101 100-101 0.21128251803028775 1101 102-103 0.1247970800610787 1101 104-105 0.2788393134356575 1101 106-107 0.2647078301913339 1101 108-109 0.20569559899962897 1101 110-111 0.19308547256558484 1101 112-113 0.25583095980867654 1101 114-115 0.2709438436828968 1101 116-117 0.24916495369662428 1101 118-119 0.11578503126577644 1101 120-121 0.0735494856063994 1101 122-123 0.06922720141591299 1101 124-125 0.05485610628666748 1102 1 0.1297521954394476 1102 2 0.20067456295507924 1102 3 0.12657659189392234 1102 4 0.22748165155906008 1102 5 0.23469491903282602 1102 6 0.26355475298593234 1102 7 0.2605654537347917 1102 8 0.32533895845979544 1102 9 0.349295166179715 1102 10-11 0.3518521224856954 1102 12-13 0.380375363628346 1102 14-15 0.4000696172804581 1102 16-17 0.312564285151268 1102 18-19 0.4130830426450345 1102 20-21 0.5647794803830166 1102 22-23 0.4953993646441148 1102 24-25 0.7818373324228602 1102 26-27 0.6587397346811059 1102 28-29 0.5261425547129974 1102 30-31 0.6466041083996927 1102 32-33 0.7277553092520108 1102 34-35 0.6580914024697009 1102 36-37 0.5613937322198979 1102 38-39 0.6121824326122862 1102 40-41 0.5861299899009964 1102 42-43 0.48524266641252467 1102 44-45 0.35094125512003416 1102 46-47 0.42044704390731624 1102 48-49 0.3879540830760817 1102 50-51 0.36971842163549695 1102 52-53 0.3847520395072195 1102 54-55 0.4100817659496627 1102 56-57 0.3946740828351878 1102 58-59 0.4366163094838882 1102 60-61 0.4784906579319639 1102 62-63 0.3983431246499052 1102 64-65 0.38569666783951106 1102 66-67 0.37533489522795094 1102 68-69 0.39903398918209376 1102 70-71 0.3169930115159474 1102 72-73 0.36964868573444676 1102 74-75 0.28441796212700865 1102 76-77 0.2374297182849361 1102 78-79 0.25698466298565137 1102 80-81 0.25821262773316533 1102 82-83 0.28054722018108436 1102 84-85 0.24069532502137747 1102 86-87 0.26389658884802714 1102 88-89 0.21077686465950407 1102 90-91 0.1491026140784797 1102 92-93 0.1263599566365059 1102 94-95 0.1425103521473403 1102 96-97 0.09684120134327046 1102 98-99 0.1275793053765426 1102 100-101 0.09697405670726766 1102 102-103 0.07561588142730358 1102 104-105 0.1261414565587664 1102 106-107 0.08934659666152456 1102 108-109 -0.010310409084834049 1102 110-111 -0.011812648070399945 1102 112-113 0.1264911542907683 1102 114-115 0.19552516099753348 1102 116-117 0.32990952050611 1102 118-119 0.08762300720982807 1102 120-121 0.029002044917319836 1102 122-123 0.14282771155045992 1102 124-125 -0.06911127096287828 1103 1 0.4317581196716347 1103 2 0.2451833498939493 1103 3 0.4906256238737612 1103 4 0.6052906784507925 1103 5 0.5765273046803259 1103 6 0.5306079917032207 1103 7 0.5746383103631487 1103 8 0.5674899454569413 1103 9 0.6769376015112414 1103 10-11 0.6110601616951499 1103 12-13 0.6505073208975531 1103 14-15 0.719036351452452 1103 16-17 0.768301759500666 1103 18-19 0.8368971038672726 1103 20-21 0.9025330422519957 1103 22-23 0.8663603886567159 1103 24-25 1.1011401066047455 1103 26-27 0.990645722075115 1103 28-29 0.9337833250338221 1103 30-31 0.9787194502064835 1103 32-33 1.1308156842465493 1103 34-35 1.0486547603929566 1103 36-37 0.9026497630360382 1103 38-39 1.1168295964190946 1103 40-41 1.0858013183390938 1103 42-43 1.0327286941546063 1103 44-45 0.8951695885865121 1103 46-47 0.894202959025904 1103 48-49 0.855611314768268 1103 50-51 0.8394341003742696 1103 52-53 0.8293910799232904 1103 54-55 0.6941409948273645 1103 56-57 0.8203572736989315 1103 58-59 0.8283061350533316 1103 60-61 0.7716634935408493 1103 62-63 0.8202848890907006 1103 64-65 0.7548911387629715 1103 66-67 0.7988043199176289 1103 68-69 0.8044449637226876 1103 70-71 0.8011519471294122 1103 72-73 0.7633768567072678 1103 74-75 0.7824877519297502 1103 76-77 0.715181723730872 1103 78-79 0.7386398128973752 1103 80-81 0.6401602016134902 1103 82-83 0.5601893876630655 1103 84-85 0.625529068273103 1103 86-87 0.6890427879052226 1103 88-89 0.6555106607313768 1103 90-91 0.6380127120322996 1103 92-93 0.610463111805938 1103 94-95 0.7534019395256308 1103 96-97 0.713529236509121 1103 98-99 0.6737630568886885 1103 100-101 0.8028722149313694 1103 102-103 0.7731498170084521 1103 104-105 0.7933020031476516 1103 106-107 0.8320838923583764 1103 108-109 0.7139059693979526 1103 110-111 0.7298560287605866 1103 112-113 0.8350764045193877 1103 114-115 0.8508555996942455 1103 116-117 0.8669074674238146 1103 118-119 0.7381598826724591 1103 120-121 0.7239652324096788 1103 122-123 0.7968289210101034 1103 124-125 0.7963253561184196 1104 1 0.4673712317719989 1104 2 0.4300741025249657 1104 3 0.5325885127881556 1104 4 0.5149985132146497 1104 5 0.4390625644398085 1104 6 0.4891844437207524 1104 7 0.4197236384777341 1104 8 0.3974732241263226 1104 9 0.6410740721326391 1104 10-11 0.537749685544938 1104 12-13 0.5438578738793112 1104 14-15 0.5903367193015967 1104 16-17 0.6242247447363738 1104 18-19 0.8088035570324479 1104 20-21 0.8012982711459742 1104 22-23 0.7951587028325093 1104 24-25 0.963229522938704 1104 26-27 0.8950998275090996 1104 28-29 0.8098377201968177 1104 30-31 0.851090068784984 1104 32-33 0.8764717624149938 1104 34-35 0.8908675884018464 1104 36-37 0.8287155087711398 1104 38-39 0.8950506169739469 1104 40-41 0.8657422903306085 1104 42-43 0.7541706798956653 1104 44-45 0.5879721429937561 1104 46-47 0.5499888061963603 1104 48-49 0.5196361420684354 1104 50-51 0.5370669398192973 1104 52-53 0.6119043029622873 1104 54-55 0.6086446350640529 1104 56-57 0.6223676840107899 1104 58-59 0.6226107401138279 1104 60-61 0.6463874053456564 1104 62-63 0.6547078521789444 1104 64-65 0.6673947125572965 1104 66-67 0.646258419311394 1104 68-69 0.61558163669131 1104 70-71 0.6456007875086556 1104 72-73 0.6851794684965569 1104 74-75 0.6739098401934172 1104 76-77 0.608477663095158 1104 78-79 0.7161986496323607 1104 80-81 0.6349833591966743 1104 82-83 0.6268153583760707 1104 84-85 0.6461712539453117 1104 86-87 0.600337563521272 1104 88-89 0.6260828554035065 1104 90-91 0.5648902375975169 1104 92-93 0.5139442391518081 1104 94-95 0.6051311274588898 1104 96-97 0.613849317609084 1104 98-99 0.5613725106317098 1104 100-101 0.5823183925821738 1104 102-103 0.5435121393506037 1104 104-105 0.6038829762666786 1104 106-107 0.6660637804533138 1104 108-109 0.6162196992110474 1104 110-111 0.662989456119945 1104 112-113 0.6961382291477634 1104 114-115 0.667754890170233 1104 116-117 0.671287907393129 1104 118-119 0.6098680909229905 1104 120-121 0.6223338204689774 1104 122-123 0.6392564283539954 1104 124-125 0.6020474790126471 1105 1 0.26843927691942326 1105 2 0.2116372444147494 1105 3 0.30971075585140895 1105 4 0.3281193472787436 1105 5 0.2768064515930462 1105 6 0.18646302921241897 1105 7 0.24247473907254857 1105 8 0.2253869392766319 1105 9 0.3083633687923566 1105 10-11 0.28826214458536725 1105 12-13 0.34723653645968255 1105 14-15 0.25129765772278034 1105 16-17 0.392320401114965 1105 18-19 0.43806436753393996 1105 20-21 0.5344877567621964 1105 22-23 0.48840270294348187 1105 24-25 0.6513962460842393 1105 26-27 0.6176316513703313 1105 28-29 0.546112549843663 1105 30-31 0.5381233749688192 1105 32-33 0.6841726067245517 1105 34-35 0.6685710312348263 1105 36-37 0.5616891113402431 1105 38-39 0.57866335969684 1105 40-41 0.5878964117522543 1105 42-43 0.4719853415129265 1105 44-45 0.4524045873298661 1105 46-47 0.45155746963878585 1105 48-49 0.44346633587022666 1105 50-51 0.40257440246149656 1105 52-53 0.4025558362644972 1105 54-55 0.4633827787126137 1105 56-57 0.43174177675702197 1105 58-59 0.44881082939007655 1105 60-61 0.3974037772817951 1105 62-63 0.48033980861688974 1105 64-65 0.5193526343132504 1105 66-67 0.5377678369151653 1105 68-69 0.5152441382890629 1105 70-71 0.5143404268563998 1105 72-73 0.5337572661575365 1105 74-75 0.5085954461076412 1105 76-77 0.5296290120979066 1105 78-79 0.5211923477795466 1105 80-81 0.4109586229381321 1105 82-83 0.4543596695171672 1105 84-85 0.5343506476793323 1105 86-87 0.4820592302028075 1105 88-89 0.556273672236582 1105 90-91 0.542003476244524 1105 92-93 0.5408367271649119 1105 94-95 0.5469572106889231 1105 96-97 0.5410100307093124 1105 98-99 0.6241796144844614 1105 100-101 0.6226350780255849 1105 102-103 0.502955480640523 1105 104-105 0.5403684621880842 1105 106-107 0.6241323071574172 1105 108-109 0.6107590223762038 1105 110-111 0.5820804411015246 1105 112-113 0.572265515179545 1105 114-115 0.6170872133244885 1105 116-117 0.7128559218239765 1105 118-119 0.6450916456832196 1105 120-121 0.6604703100793401 1105 122-123 0.6559820386478172 1105 124-125 0.6693112734810889 1106 1 0.3501644270053994 1106 2 0.38554466623582684 1106 3 0.37020612667537023 1106 4 0.2824860967083467 1106 5 0.2536731369806233 1106 6 0.30996701056240994 1106 7 0.20972698658337663 1106 8 0.25094972298128226 1106 9 0.2520272694506147 1106 10-11 0.2738012734123103 1106 12-13 0.23341504143109404 1106 14-15 0.30850181483322814 1106 16-17 0.34173029787193343 1106 18-19 0.45970085917709724 1106 20-21 0.4655691904148398 1106 22-23 0.4586398551118265 1106 24-25 0.5933551113154181 1106 26-27 0.5848301157215019 1106 28-29 0.507195334752673 1106 30-31 0.5240162221606681 1106 32-33 0.6358693197236462 1106 34-35 0.6536323640689332 1106 36-37 0.5314782121525781 1106 38-39 0.5797640933695547 1106 40-41 0.501492794513041 1106 42-43 0.46133318003087 1106 44-45 0.45545371612159613 1106 46-47 0.4327808337717727 1106 48-49 0.35496256516082525 1106 50-51 0.294856324800314 1106 52-53 0.2285497715151621 1106 54-55 0.21381835553214046 1106 56-57 0.22288367822117294 1106 58-59 0.22857637852037982 1106 60-61 0.2006729314013853 1106 62-63 0.29199610047564306 1106 64-65 0.2766882991404884 1106 66-67 0.29604816712677007 1106 68-69 0.27837927885796887 1106 70-71 0.34412231125563153 1106 72-73 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-2.345291145271993 2216 30-31 -5.061312035720519 2216 32-33 -7.789235346811942 2216 34-35 -8.170873845329673 2216 36-37 -7.367613245100102 2216 38-39 -7.354259327095203 2216 40-41 -7.185820405708007 2216 42-43 -7.675148938163332 2216 44-45 -5.78539021230911 2216 46-47 -2.918604569266705 2216 48-49 -2.3567320011198234 2216 50-51 -2.3137373550359897 2216 52-53 -2.326782659272837 2216 54-55 -2.3697279720266806 2216 56-57 -2.278441315771296 2216 58-59 -2.153983652273766 2216 60-61 -2.161140642621497 2216 62-63 -2.098169169288724 2216 64-65 -2.1737091788298812 2216 66-67 -2.160519316644809 2216 68-69 -2.3670679139469115 2216 70-71 -2.3984360892123533 2216 72-73 -2.325771867702656 2216 74-75 -2.3222222147205116 2216 76-77 -2.3994843937337507 2216 78-79 -2.3431502875494417 2216 80-81 -2.3797055048193805 2216 82-83 -2.4302017510489726 2216 84-85 -2.3889114041897557 2216 86-87 -2.3838650256742326 2216 88-89 -2.3104959694543616 2216 90-91 -2.3437962128736345 2216 92-93 -2.3456215373757274 2216 94-95 -2.3354373927838523 2216 96-97 -2.4509400007213067 2216 98-99 -2.472116250371215 2216 100-101 -2.422989920445687 2216 102-103 -2.4969848990217898 2216 104-105 -2.494260558668639 2216 106-107 -2.511815056951022 2216 108-109 -2.51150792019504 2216 110-111 -2.643124224816681 2216 112-113 -2.635038679401667 2216 114-115 -2.7138943269959803 2216 116-117 -2.5963068679920163 2216 118-119 -2.4856728766080636 2216 120-121 -2.3444902548890916 2216 122-123 -2.3866371775679376 2216 124-125 -2.1963893296932824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25671.0 3 5039.0 4 3555.0 5 3078.0 6 3792.0 7 4456.0 8 4178.0 9 4343.0 10 5205.0 11 6307.0 12 7100.0 13 6992.0 14 6700.0 15 6115.0 16 5464.0 17 4928.0 18 4656.0 19 4329.0 20 4337.0 21 4464.0 22 5110.0 23 7269.0 24 12198.0 25 18099.0 26 20439.0 27 18454.0 28 21271.0 29 25683.0 30 30803.0 31 38059.0 32 50156.0 33 67346.0 34 99416.0 35 160912.0 36 336282.0 37 628947.0 38 255378.0 39 882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84051230613955 18.300353095035064 12.618633356260677 24.240501242564704 2 15.899820657332361 20.223507821521434 39.427888019628035 24.448783501518175 3 19.007069551256464 25.414277582024585 28.826383529808314 26.752269336910633 4 12.603950239546016 16.573651921011905 36.68132501140074 34.14107282804134 5 13.533046046730771 38.941892032205736 33.81252696899068 13.712534952072811 6 32.327992214156595 36.64129104805338 17.282767771364913 13.747948966425113 7 28.769046546170664 32.92981984011749 20.671187352926452 17.629946260785392 8 26.341429832800756 35.78832520693246 19.55415969329508 18.316085266971697 9 26.79068569109948 16.614425621442436 19.011272591861662 37.58361609559642 10-11 24.895871363451448 27.215386942326347 27.30350381257367 20.58523788164853 12-13 25.353271839120623 24.748508628000344 27.20065381498121 22.69756571789782 14-15 22.73710086140733 26.131876608896142 24.509673783471754 26.621348746224776 16-17 22.927856085600943 28.956894942113177 25.365632572252917 22.749616400032963 18-19 23.036182947793577 27.77474401662327 26.826776236731426 22.362296798851723 20-21 24.077293904870444 26.88778457433977 26.4842591711208 22.550662349668986 22-23 23.657921078451572 26.90633706097535 26.37728800350943 23.058453857063647 24-25 23.28598367053376 27.073174357172974 26.620540057887748 23.020301914405515 26-27 23.0060416066266 27.319407080142245 26.417273804882058 23.2572775083491 28-29 23.146928483450033 27.430103071060174 26.390786480460886 23.03218196502891 30-31 23.249203952464033 26.99011883992208 26.343188414867903 23.417488792745992 32-33 22.909631709650693 27.185251383478164 26.39624963393667 23.50886727293447 34-35 23.46274312096733 27.33442162293556 26.208644364815054 22.99419089128206 36-37 23.703891097772324 26.8072498901354 26.157529539559732 23.331329472532545 38-39 23.166700445542062 27.11131665361159 26.25270720079546 23.46927570005089 40-41 23.43154152092169 26.97378011682547 26.371476205266298 23.22320215698654 42-43 23.18955243730393 26.879943256049255 26.295840428500277 23.63466387814654 44-45 23.337509107342253 27.072236544211485 26.37389831799058 23.21635603045568 46-47 23.601374484418397 27.295125336821442 25.813880289886136 23.289619888874025 48-49 23.200713693192768 27.24533151346089 26.037414651103173 23.516540142243166 50-51 23.152486868158476 27.28703181917631 26.17238666379122 23.388094648874 52-53 23.542039300013013 26.947183354773195 26.152791428505118 23.357985916708675 54-55 23.282799583476653 27.100721613915823 26.13475359483482 23.4817252077727 56-57 23.25532333243453 27.41627685135976 26.017479233175184 23.31092058303053 58-59 23.058671965868136 26.99760597099 26.174221679784278 23.769500383357585 60-61 22.979966846137074 27.08140118364924 26.400119554206775 23.53851241600691 62-63 23.036842423711345 27.12167065166139 26.556077847184838 23.285409077442427 64-65 22.951907658615596 27.219408317723794 26.21273952487361 23.615944498787005 66-67 22.94339217203906 27.355590998324757 26.0915167534887 23.60950007614748 68-69 23.24974716034598 26.987470941043373 26.43325789436075 23.329524004249897 70-71 23.138470011646216 26.96620511213068 26.200021800798073 23.695303075425027 72-73 23.0990610209881 27.12282091648962 26.12751411649995 23.650603946022333 74-75 22.93838392925828 27.518107226070754 26.055134629100124 23.488374215570847 76-77 23.15477045734338 27.208179990669706 26.17240804720742 23.464641504779493 78-79 23.32217556636794 27.232626923945507 26.136086857426584 23.30911065225997 80-81 23.302487929350683 27.298560649226864 26.047698459989853 23.351252961432596 82-83 23.318933505234217 27.311665268928913 26.185651909059427 23.183749316777444 84-85 23.157723435600676 27.36612276097576 26.14627948321874 23.32987432020482 86-87 23.273259842470402 27.129456908068967 26.226516013092432 23.3707672363682 88-89 23.166185670889362 27.292292358855967 26.113807069190635 23.427714901064032 90-91 23.145908902443157 27.39127827077884 26.148915585990366 23.313897240787632 92-93 23.28157910589202 27.01337018254494 26.235505161123594 23.469545550439445 94-95 23.100500518146063 26.90651414171073 26.471031540935623 23.52195379920758 96-97 23.2467154518102 26.741967062465744 26.395349504299343 23.61596798142472 98-99 22.935705397107586 27.062664435032076 26.17079307796819 23.83083708989215 100-101 22.90214085303419 27.012236501066955 26.405392844419247 23.680229801479605 102-103 22.838657015929975 26.856395525472703 26.492002414857367 23.81294504373995 104-105 22.76347428762186 27.164362054486563 26.246003223501308 23.82616043439027 106-107 22.888158212221718 27.225534323616145 25.996554139271726 23.88975332489041 108-109 22.812257646947767 27.267772644087117 25.964209848862964 23.955759860102148 110-111 22.738837973122212 27.301646906655296 26.25216929850982 23.70734582171267 112-113 22.826939116582505 27.519230638827036 26.182696676217436 23.471133568373027 114-115 22.742455461588204 27.82232684100406 25.961335047648408 23.473882649759325 116-117 22.804939042064614 27.67719075611112 26.12102776782735 23.396842433996916 118-119 22.680957906687162 27.740074669949504 25.90614386186949 23.67282356149384 120-121 22.608543775702653 27.933156917498792 25.994662620380833 23.463636686417722 122-123 22.72069540033823 27.93132066180713 25.93883636037378 23.409147577480862 124-125 22.748335595791175 27.981480372077307 25.8168196583475 23.453364373784023 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1333.0 1 1072.5 2 760.0 3 604.0 4 450.5 5 358.5 6 309.0 7 315.0 8 327.5 9 312.0 10 287.5 11 281.5 12 284.5 13 311.5 14 362.5 15 422.0 16 481.5 17 548.0 18 666.5 19 852.5 20 1149.5 21 1573.0 22 2146.5 23 3047.5 24 4195.5 25 5764.5 26 8338.0 27 11266.5 28 13678.0 29 16268.5 30 19193.5 31 23569.5 32 29113.0 33 32295.5 34 34163.0 35 37308.5 36 42319.5 37 47022.0 38 48870.5 39 50295.0 40 52772.0 41 55609.0 42 57614.0 43 59138.0 44 61584.5 45 65061.5 46 67285.0 47 67970.5 48 68282.5 49 68179.5 50 68633.0 51 69825.0 52 70791.0 53 72426.5 54 73751.5 55 70621.5 56 66092.0 57 61394.5 58 57532.5 59 51767.0 60 41967.0 61 34408.0 62 28908.0 63 21817.0 64 15513.0 65 11966.0 66 8986.5 67 6882.5 68 5282.5 69 3919.5 70 2865.5 71 1899.5 72 1394.5 73 1206.0 74 902.0 75 612.0 76 447.5 77 287.5 78 179.5 79 105.0 80 61.5 81 42.0 82 33.5 83 30.0 84 23.0 85 14.5 86 12.5 87 9.5 88 6.5 89 8.0 90 6.5 91 7.0 92 6.0 93 3.0 94 5.0 95 4.0 96 1.5 97 3.0 98 2.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06336662993314429 2 0.050588996736748944 3 0.04610378671678976 4 0.04542578985330755 5 0.04485210019959185 6 0.00453736362484243 7 2.6076802441623166E-4 8 0.0 9 9.909184927816804E-4 10-11 0.0038072131564769827 12-13 0.002659833849045563 14-15 0.007484042300745848 16-17 0.002659833849045563 18-19 0.00526751409320788 20-21 0.009674493705842195 22-23 0.015333159835674421 24-25 0.004250518797984576 26-27 0.015150622218583059 28-29 0.007484042300745848 30-31 0.006675661425055531 32-33 0.002790217861253679 34-35 0.017601841648095636 36-37 0.014629086169750595 38-39 0.027876101810095165 40-41 0.005658666129832227 42-43 0.0014603009367308974 44-45 2.0861441953298534E-4 46-47 0.00206006739288823 48-49 0.0032596003052028956 50-51 0.0016428385538222595 52-53 0.0017992993684719984 54-55 0.005919434154248458 56-57 0.0027119874539288094 58-59 0.007457965498304226 60-61 0.015176699021024682 62-63 0.01152594667919744 64-65 0.0025816034417206935 66-67 0.004224441995542953 68-69 0.009909184927816804 70-71 0.0027119874539288094 72-73 0.003363907514969388 74-75 8.083808756903183E-4 76-77 1.3038401220811583E-4 78-79 0.0033378307125277655 80-81 0.0028945250710201717 82-83 0.0015124545416141436 84-85 0.010404644174207642 86-87 0.006388816598197676 88-89 0.0019036065782384913 90-91 9.387648878984341E-4 92-93 0.0025816034417206935 94-95 0.0 96-97 0.0013559937269644047 98-99 0.022556434112004038 100-101 0.014603009367308972 102-103 0.006023741364014952 104-105 0.012673325986628858 106-107 0.03236131183005435 108-109 7.301504683654487E-4 110-111 0.001590684948939013 112-113 0.015124545416141437 114-115 0.03194408299098838 116-117 0.05051076632942407 118-119 0.3583995727576688 120-121 0.11687622854335503 122-123 0.018723144153085434 124-125 0.3204056716002238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1917413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.79037872536607 #Duplication Level Percentage of deduplicated Percentage of total 1 82.74027287089368 56.91734706628907 2 10.235013372072018 14.081408922480405 3 3.1938753013951002 6.59123674753685 4 1.4016528051949841 3.8568090924333887 5 0.7554680978922355 2.598446828446941 6 0.43052299768188235 1.7769504036309953 7 0.2718628815458656 1.3091085408036602 8 0.18717465769704225 1.0300652472616234 9 0.1271105511799183 0.7869584660090946 >10 0.6031059799277271 7.363332492603162 >50 0.03857790033843003 1.811088817945625 >100 0.015202557289707347 1.675795293086977 >500 0.0 0.0 >1k 1.600268913609466E-4 0.20145208147221427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.2153604883246336E-5 7 5.2153604883246336E-5 0.0 0.0 0.0 1.0430720976649267E-4 8 5.2153604883246336E-5 0.0 0.0 0.0 1.0430720976649267E-4 9 5.2153604883246336E-5 0.0 0.0 0.0 1.56460814649739E-4 10-11 5.2153604883246336E-5 0.0 0.0 0.0 1.56460814649739E-4 12-13 5.2153604883246336E-5 0.0 0.0 0.0 3.911520366243475E-4 14-15 5.2153604883246336E-5 0.0 0.0 0.0 6.779968634822023E-4 16-17 5.2153604883246336E-5 0.0 0.0 2.0861441953298532E-4 8.344576781319414E-4 18-19 7.82304073248695E-5 0.0 0.0 3.3899843174110117E-4 9.38764887898434E-4 20-21 1.0430720976649267E-4 0.0 0.0 5.997664561573329E-4 0.0011213025049897963 22-23 1.0430720976649267E-4 0.0 0.0 7.82304073248695E-4 0.0012516865171979118 24-25 1.56460814649739E-4 0.0 0.0 0.0014342241342892741 0.0013038401220811582 26-27 1.56460814649739E-4 0.0 0.0 0.002372989022187708 0.0013299169245227815 28-29 1.56460814649739E-4 0.0 0.0 0.0042765956004261994 0.0013559937269644047 30-31 1.56460814649739E-4 0.0 0.0 0.011004410630364975 0.0013559937269644047 32-33 1.56460814649739E-4 0.0 0.0 0.02612895604650641 0.001408147331847651 34-35 1.56460814649739E-4 0.0 0.0 0.05030215190989109 0.00156460814649739 36-37 1.56460814649739E-4 0.0 0.0 0.08973027720162531 0.001590684948939013 38-39 1.56460814649739E-4 0.0 0.0 0.1464212457097141 0.0016689153562638825 40-41 1.56460814649739E-4 0.0 0.0 0.20853618912566046 0.001721068961147129 42-43 1.56460814649739E-4 0.0 0.0 0.27046859492451547 0.001747145763588752 44-45 1.56460814649739E-4 0.0 0.0 0.34408340821721767 0.001903606578238491 46-47 1.56460814649739E-4 0.0 0.0 0.4301107794721325 0.0021382978002130997 48-49 2.0861441953298534E-4 0.0 0.0 0.5295937807869249 0.0022947586148628386 50-51 2.0861441953298534E-4 0.0 0.0 0.6416718776810213 0.002346912219746085 52-53 2.0861441953298534E-4 0.0 0.0 0.7467874683232042 0.002503373034395824 54-55 2.0861441953298534E-4 0.0 0.0 0.8611342470297219 0.00263375704660394 56-57 2.0861441953298534E-4 0.0 0.0 0.9963685444919795 0.002659833849045563 58-59 2.0861441953298534E-4 0.0 0.0 1.146075467309338 0.002685910651487186 60-61 2.0861441953298534E-4 0.0 0.0 1.3161222960311627 0.0027119874539288094 62-63 2.0861441953298534E-4 0.0 0.0 1.4866385071969366 0.0029206018734617947 64-65 2.0861441953298534E-4 0.0 0.0 1.658145636855492 0.0029988322807866638 66-67 3.12921629299478E-4 0.0 0.0 1.8555731081410212 0.003024909083228287 68-69 3.12921629299478E-4 0.0 0.0 2.0945670025184975 0.003024909083228287 70-71 3.12921629299478E-4 0.0 0.0 2.354552722861481 0.003181369897878026 72-73 3.3899843174110117E-4 0.0 0.0 2.625360316217737 0.0036768291442688663 74-75 3.6507523418272434E-4 0.0 0.0 2.89337769171274 0.0038854435638018517 76-77 3.6507523418272434E-4 0.0 0.0 3.1909661611765436 0.003937597168685098 78-79 3.6507523418272434E-4 0.0 0.0 3.528087063141848 0.003989750773568345 80-81 3.6507523418272434E-4 0.0 0.0 3.9006984932302013 0.004067981180893214 82-83 3.6507523418272434E-4 0.0 0.0 4.296413970281833 0.004433056415075938 84-85 3.911520366243475E-4 0.0 0.0 4.694111284319028 0.004485210019959184 86-87 4.172288390659707E-4 0.0 0.0 5.097910570127563 0.004615594032167301 88-89 4.172288390659707E-4 0.0 0.0 5.536000851146832 0.004745978044375416 90-91 4.172288390659707E-4 0.0 0.0 6.03239886242557 0.004798131649258663 92-93 4.172288390659707E-4 0.0 0.0 6.5606888030904145 0.005058899673674894 94-95 4.172288390659707E-4 0.0 0.0 7.075940342534446 0.005241437290766256 96-97 4.172288390659707E-4 0.0 0.0 7.600996759696528 0.005606512524948981 98-99 4.172288390659707E-4 0.0 0.0 8.146314852355752 0.0058933573518068355 100-101 4.4330564150759386E-4 0.0 0.0 8.76383961097583 0.0058933573518068355 102-103 4.69382443949217E-4 0.0 0.0 9.427702847534672 0.006049818166456574 104-105 5.215360488324633E-4 0.0 0.0 10.089297402281094 0.006154125376223067 106-107 5.215360488324633E-4 0.0 0.0 10.740278698433775 0.00618020217866469 108-109 5.215360488324633E-4 0.0 0.0 11.399474187355567 0.006310586190872806 110-111 5.215360488324633E-4 0.0 0.0 12.122662149469104 0.006388816598197676 112-113 5.215360488324633E-4 0.0 0.0 12.891588823065245 0.006414893400639299 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3655 0.0 47.855133 1 GGTATCA 2460 0.0 44.257263 1 CAACGCA 4445 0.0 38.279198 5 ATCAACG 4520 0.0 37.37689 3 AACGCAG 4655 0.0 36.665775 6 GTACATG 9585 0.0 36.062233 1 TCAACGC 4690 0.0 36.023018 4 TACATGG 9495 0.0 35.711094 2 TATCAAC 4900 0.0 35.206688 2 ACATGGG 9990 0.0 32.810238 3 ACGCAGA 5585 0.0 30.240047 7 CGCAGAG 5630 0.0 29.78709 8 CATGGGA 5605 0.0 27.807394 4 TGGTATC 1335 0.0 25.84461 2 CATGGGG 4845 0.0 25.784584 4 GTGGTAT 1525 0.0 25.74795 1 CATGGGT 1395 0.0 23.880774 4 GCAGAGT 7005 0.0 23.433306 9 TAGACCG 210 2.796336E-6 22.662369 4 GTATAGG 615 0.0 22.249554 1 >>END_MODULE