FastQCFastQC Report
Thu 26 May 2016
SRR1364185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917413
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67660.3528712906400447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60940.31782406815850317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.18634483024783915No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39550.072.563411
GTATCAA70800.055.5885431
ATCAACG92600.042.156123
CAACGCA94650.041.2951745
TCAACGC95600.041.019974
TATCAAC95850.040.9140472
AACGCAG98600.039.7594536
TGGTATC15850.034.9166682
GTGGTAT16750.034.4805371
ACGCAGA117950.033.337687
CGCAGAG119800.032.7235578
GTACATG94500.032.0703051
TACATGG94600.031.7043252
ACATGGG96550.029.7072543
GCAGAGT135650.028.5929959
GAGTACT82950.026.4645512-13
CAGAGTA126450.026.1805210-11
AGAGTAC121650.025.67314310-11
CATGGGG46200.024.6013664
AGTACTT89150.024.3237612-13
Produced by FastQC (version 0.11.5)