FastQCFastQC Report
Thu 26 May 2016
SRR1364184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925995
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59750.31022925812372304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.055.5549741
GTATCAA50500.048.301711
ATCAACG57650.040.656793
TCAACGC57700.040.416414
CAACGCA58000.040.309935
AACGCAG60350.038.7221766
GTACATG124850.036.882681
TATCAAC67800.035.27222
TACATGG130250.034.3003352
ACATGGG127350.032.7457733
ACGCAGA72700.031.7335917
CGCAGAG72750.031.3848558
CATGGGA86250.030.7626154
GCAGAGT81750.027.0603529
CATGGGG39450.025.1835484
ATGGGAT35200.025.182055
GAGTACA92650.024.3368191
ATGGGAG39550.023.4652735
AGTACAT92350.023.1256392
CAGAGTA81000.022.06163610-11
Produced by FastQC (version 0.11.5)