FastQCFastQC Report
Thu 26 May 2016
SRR1364184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925995
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124040.6440307477433742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108900.5654220286137814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66880.34724908423957485No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.15508866845448716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.1151612543127059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.074.227671
GTATCAA108700.054.5535471
ATCAACG140950.041.8428543
TCAACGC141350.041.808654
CAACGCA143300.041.1449055
TATCAAC146200.040.259922
AACGCAG148550.039.9269376
ACGCAGA168800.034.996167
CGCAGAG169250.034.7273648
TGGTATC24650.033.801432
GTACATG119350.033.5726661
TACATGG124150.031.9265142
GCAGAGT183350.031.7971869
ACATGGG120550.031.4474123
GTGGTAT29200.029.157241
CATGGGA85100.028.5327264
GAGTACT121700.028.52290712-13
CAGAGTA182000.027.16271410-11
AGAGTAC178050.026.37871710-11
AGTACTT126300.026.28296712-13
Produced by FastQC (version 0.11.5)