FastQCFastQC Report
Thu 26 May 2016
SRR1364183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488450
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22200.048.511191
GTATCAA37000.043.0972331
ATCAACG39300.038.9041373
CAACGCA39200.038.701415
TCAACGC39400.038.5010834
AACGCAG40550.037.2520076
TATCAAC47150.033.312222
GTACATG79550.031.7881341
TACATGG78100.031.2330342
ACGCAGA48800.030.7099297
ACATGGG79150.029.9148863
CGCAGAG50500.029.205088
CATGGGG46200.024.2072184
GTGTAGC22500.023.7999631
GCAGAGT67100.021.3608639
TAGCCCT25500.020.2958894
GAGTACA58000.020.2094541
CAGAGTA60550.019.84090410-11
AGAGTAC55850.019.80678710-11
CATGGGA38050.019.6990324
Produced by FastQC (version 0.11.5)