FastQCFastQC Report
Thu 26 May 2016
SRR1364183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488450
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.2606441760935522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.2508388756052965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.13610882276115654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.073.831141
GTATCAA80150.050.433951
ATCAACG100200.040.087543
TCAACGC102150.039.3805434
CAACGCA103300.038.8183365
TATCAAC104950.038.3298952
AACGCAG107050.037.9016086
TGGTATC19150.035.735712
ACGCAGA123450.032.8176277
GTGGTAT21850.032.695391
CGCAGAG123600.032.6334048
TACATGG77200.029.4455322
GTACATG82100.028.2799191
GCAGAGT143400.027.9201099
ACATGGG76750.027.6022823
GAGTACT102950.026.87116612-13
CAGAGTA136700.026.80793810-11
AGAGTAC131000.024.84089510-11
GTACTTT115100.023.95710414-15
TAGGACG2251.9112667E-723.8030914
Produced by FastQC (version 0.11.5)