FastQCFastQC Report
Thu 26 May 2016
SRR1364182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470508
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77560.5274367769505504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34450.043.0158541
GGTATCA23450.040.860291
GTACATG80650.037.265371
TACATGG81600.036.6077352
ATCAACG40200.035.081783
TCAACGC40500.034.6738054
ACATGGG82400.034.591363
CAACGCA41700.033.8175435
AACGCAG42100.033.6192676
TATCAAC46300.031.2309072
CATGGGG41850.030.9959934
ACGCAGA47350.029.512897
CGCAGAG48500.028.8130998
GAACAAA17300.028.5529121
CATGGGA44700.028.0879124
ATGGGAG15450.025.4182245
GTATAGG2601.1023076E-925.1789591
CCAAGAC13050.024.623093
GCAGAGT54500.024.2250469
ATGGGAT15300.022.945125
Produced by FastQC (version 0.11.5)