##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470508 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.563542666887905 31.0 31.0 34.0 26.0 34.0 2 30.614070103664854 33.0 31.0 34.0 26.0 34.0 3 30.717495586559203 33.0 31.0 34.0 26.0 34.0 4 33.96643336860459 37.0 35.0 37.0 28.0 37.0 5 34.21587437810607 37.0 35.0 37.0 30.0 37.0 6 34.079935641288586 37.0 35.0 37.0 30.0 37.0 7 34.08728752240722 37.0 35.0 37.0 30.0 37.0 8 33.98210414360207 37.0 35.0 37.0 30.0 37.0 9 35.36370288362933 39.0 37.0 39.0 28.0 39.0 10-11 35.413188503564754 39.0 37.0 39.0 29.0 39.0 12-13 35.36155124623599 39.0 37.0 39.0 28.5 39.0 14-15 36.63945554869474 40.0 37.0 41.0 28.5 41.0 16-17 36.685166282672384 40.0 37.0 41.0 29.0 41.0 18-19 36.5576419849467 40.0 37.0 41.0 28.5 41.0 20-21 36.52196859860674 40.0 37.0 41.0 27.5 41.0 22-23 36.56355354748155 40.0 37.0 41.0 27.5 41.0 24-25 36.43838183811309 40.0 37.0 41.0 27.5 41.0 26-27 36.450050934779 40.0 36.0 41.0 28.0 41.0 28-29 36.523295691012905 40.0 36.0 41.0 29.0 41.0 30-31 36.40473394228389 40.0 36.0 41.0 29.0 41.0 32-33 36.21270982544807 40.0 36.0 41.0 27.5 41.0 34-35 36.154794805604595 40.0 36.0 41.0 28.0 41.0 36-37 36.141914902877105 40.0 36.0 41.0 28.0 41.0 38-39 35.94518186912278 40.0 35.5 41.0 27.0 41.0 40-41 35.90087711185522 40.0 35.0 41.0 27.0 41.0 42-43 35.79347511200211 40.0 35.0 41.0 27.0 41.0 44-45 35.73831152227665 40.0 35.0 41.0 27.0 41.0 46-47 35.61312247196207 40.0 35.0 41.0 27.0 41.0 48-49 35.478451664322805 39.0 35.0 41.0 27.0 41.0 50-51 35.32627398150843 39.0 35.0 41.0 27.0 41.0 52-53 35.1333324946209 39.0 35.0 41.0 26.5 41.0 54-55 34.90865469620022 39.0 34.0 41.0 26.0 41.0 56-57 34.682017370867754 38.0 34.0 40.5 26.0 41.0 58-59 34.42748560361453 38.0 34.0 40.0 26.0 41.0 60-61 34.151569049607346 37.0 34.0 40.0 25.5 41.0 62-63 33.84072987022172 37.0 33.0 40.0 25.0 41.0 64-65 33.52695361058899 36.0 33.0 39.5 24.0 41.0 66-67 33.21655883545006 36.0 33.0 39.0 23.0 41.0 68-69 32.98859679784129 35.0 33.0 39.0 23.0 41.0 70-71 32.83192032957318 35.0 33.0 38.5 24.0 40.5 72-73 32.55240059897668 35.0 33.0 37.5 23.0 40.0 74-75 32.24804863353344 35.0 33.0 37.0 22.5 39.0 76-77 31.954205621458705 35.0 33.0 36.5 22.0 39.0 78-79 31.645442595348 35.0 32.5 36.0 20.5 39.0 80-81 31.390623512418838 35.0 32.0 36.0 20.0 37.0 82-83 31.157949837743146 35.0 32.0 35.0 19.5 37.0 84-85 30.949366477435007 35.0 32.0 35.0 18.5 36.5 86-87 30.770422874272022 35.0 32.0 35.0 18.0 36.0 88-89 30.61752435212865 35.0 32.0 35.0 17.0 36.0 90-91 30.49576948918333 35.0 32.0 35.0 15.0 36.0 92-93 30.380656208602744 35.0 32.0 35.0 11.0 35.0 94-95 30.259413413595844 35.0 32.0 35.0 8.5 35.0 96-97 30.176696760575254 35.0 31.0 35.0 7.0 35.0 98-99 30.105771950917642 35.0 31.0 35.0 7.0 35.0 100-101 30.005604185764376 35.0 31.0 35.0 3.5 35.0 102-103 29.00861505003713 33.5 29.5 34.5 2.0 35.0 104-105 29.708422531533323 34.0 31.0 35.0 2.0 35.0 106-107 29.827470506790853 35.0 31.0 35.0 2.0 35.0 108-109 29.78574751038417 35.0 31.0 35.0 2.0 35.0 110-111 29.715302466902592 35.0 31.0 35.0 2.0 35.0 112-113 29.893109048029658 35.0 31.5 35.0 2.0 35.0 114-115 29.891126399856375 35.0 32.0 35.0 2.0 35.0 116-117 29.805214592508168 35.0 32.0 35.0 2.0 35.0 118-119 29.559674615847044 35.0 31.0 35.0 2.0 35.0 120-121 29.507224034143302 35.0 31.0 35.0 2.0 35.0 122-123 29.36803233984446 35.0 31.0 35.0 2.0 35.0 124-125 28.467850225908325 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5598073926324361 1101 2 0.5747329947615363 1101 3 0.5393885014867834 1101 4 0.4293630011053793 1101 5 0.3721817364951434 1101 6 0.324678995425856 1101 7 0.39293713916051587 1101 8 0.4218480122264978 1101 9 0.41879608242886235 1101 10-11 0.6157721565210821 1101 12-13 0.5247906144426011 1101 14-15 0.66669370990423 1101 16-17 0.5973815915052967 1101 18-19 0.7189929184435542 1101 20-21 0.8386756891157816 1101 22-23 0.7259868077779643 1101 24-25 1.0052051842242946 1101 26-27 0.9309005944146733 1101 28-29 0.7860017889818565 1101 30-31 0.8399879796437446 1101 32-33 1.0339188187974173 1101 34-35 0.9419800532004814 1101 36-37 0.8901762582198529 1101 38-39 0.8518753943653721 1101 40-41 0.7729934136834302 1101 42-43 0.6667583932455656 1101 44-45 0.6663044401601184 1101 46-47 0.6787920797121174 1101 48-49 0.6206371105269213 1101 50-51 0.5891112367390718 1101 52-53 0.5599264762358587 1101 54-55 0.5087417978891935 1101 56-57 0.3542763219252407 1101 58-59 0.3662021311426642 1101 60-61 0.3830799149085493 1101 62-63 0.33146207816442796 1101 64-65 0.3141616573866344 1101 66-67 0.24900601175242798 1101 68-69 0.2684499754110945 1101 70-71 0.29386352838965735 1101 72-73 0.3032259523544667 1101 74-75 0.32291687990817763 1101 76-77 0.444796626998766 1101 78-79 0.3632971001535381 1101 80-81 0.3806899566457318 1101 82-83 0.36939741751557875 1101 84-85 0.2676214849351979 1101 86-87 0.3056370038213032 1101 88-89 0.3365008816852537 1101 90-91 0.4258295079642629 1101 92-93 0.40101910809756447 1101 94-95 0.34518921469957675 1101 96-97 0.2592415850546459 1101 98-99 0.3435206229268921 1101 100-101 0.2321365430647262 1101 102-103 0.17738287263996355 1101 104-105 0.25298773408813346 1101 106-107 0.29385218810486435 1101 108-109 0.1450444004306739 1101 110-111 0.17608097890704855 1101 112-113 0.34587621142637914 1101 114-115 0.2197706992737274 1101 116-117 0.19732285658542992 1101 118-119 0.19889912227606743 1101 120-121 0.11367263879412093 1101 122-123 0.13359458364893229 1101 124-125 -0.039006623537183316 1102 1 0.11305182637414646 1102 2 0.20203433360677892 1102 3 0.04824267337939503 1102 4 0.14385483859772563 1102 5 0.11763190355814146 1102 6 0.027542311467577463 1102 7 0.16914228383139118 1102 8 0.06413317990825362 1102 9 0.1536960285807254 1102 10-11 0.18185385183428338 1102 12-13 0.15764265916665465 1102 14-15 0.23352760680344176 1102 16-17 0.23262803869900495 1102 18-19 0.4098139854393281 1102 20-21 0.3807073357649955 1102 22-23 0.41663261749002345 1102 24-25 0.698920003164119 1102 26-27 0.6368667001911419 1102 28-29 0.5448143205465641 1102 30-31 0.49679809640388584 1102 32-33 0.6646915228099388 1102 34-35 0.637988602009969 1102 36-37 0.5817130706109381 1102 38-39 0.6692138248761879 1102 40-41 0.49643340645908296 1102 42-43 0.468558125008272 1102 44-45 0.29307866931236504 1102 46-47 0.31872130787770203 1102 48-49 0.29776737284426247 1102 50-51 0.24118706561571912 1102 52-53 0.2343415884975144 1102 54-55 0.32020041342354943 1102 56-57 0.30195098554163025 1102 58-59 0.16829016782628514 1102 60-61 0.22496038542752927 1102 62-63 0.3233908782206143 1102 64-65 0.3569130629567354 1102 66-67 0.3522272004918108 1102 68-69 0.37429768218291315 1102 70-71 0.3588879941726759 1102 72-73 0.19550560143854057 1102 74-75 0.19407100263509847 1102 76-77 0.10545705263315597 1102 78-79 0.09857297986818025 1102 80-81 0.10014083945413077 1102 82-83 0.06820506595749265 1102 84-85 0.12131275785828066 1102 86-87 0.039685413629023 1102 88-89 0.08881685317584598 1102 90-91 0.062047228348628636 1102 92-93 0.15017643495574262 1102 94-95 0.19399268238568013 1102 96-97 0.15747596825938004 1102 98-99 0.13404970593663634 1102 100-101 0.10895943621464 1102 102-103 -0.005545261839898785 1102 104-105 0.15873978516693654 1102 106-107 0.15242725273935065 1102 108-109 0.184946871759049 1102 110-111 0.3187193368399477 1102 112-113 0.22703906357144277 1102 114-115 0.23699741998920132 1102 116-117 0.2311324616210655 1102 118-119 0.19453608550882961 1102 120-121 0.17344640973552927 1102 122-123 0.20358545287823304 1102 124-125 0.07006294089495313 1103 1 0.2688199990610549 1103 2 0.0010249886893305415 1103 3 0.23408412113785104 1103 4 0.1408088902656104 1103 5 0.24974647369620584 1103 6 0.15930927285528895 1103 7 0.14907864797181247 1103 8 0.11014911446397235 1103 9 0.21275027687147485 1103 10-11 0.08233431963461868 1103 12-13 0.10412861193451306 1103 14-15 0.13409135142403983 1103 16-17 0.08480816400876279 1103 18-19 0.20221734807415714 1103 20-21 0.33373377109057145 1103 22-23 0.2587607720927494 1103 24-25 0.5230857421632678 1103 26-27 0.48491188079840697 1103 28-29 0.37229972311161674 1103 30-31 0.3025113554385186 1103 32-33 0.5066582419377994 1103 34-35 0.5114710658949164 1103 36-37 0.4384497495185542 1103 38-39 0.5954396255492256 1103 40-41 0.506947718129787 1103 42-43 0.49046749935517653 1103 44-45 0.4360165603722024 1103 46-47 0.29850529996161157 1103 48-49 0.14517619485578592 1103 50-51 0.10834209058810984 1103 52-53 0.07579286569310284 1103 54-55 0.07475174222729919 1103 56-57 0.15779369151581335 1103 58-59 0.268941774290532 1103 60-61 0.12623438216044036 1103 62-63 0.07192420387669785 1103 64-65 0.16938848509877857 1103 66-67 0.20174950503119504 1103 68-69 0.21127003129299737 1103 70-71 0.3206318385121989 1103 72-73 0.2915001036756024 1103 74-75 0.3577479141633404 1103 76-77 0.3210869708704607 1103 78-79 0.2355514067999671 1103 80-81 0.28261997226626434 1103 82-83 0.30190957292886367 1103 84-85 0.22409245494817753 1103 86-87 0.26333176544320125 1103 88-89 0.23453401481178204 1103 90-91 0.26310647538115717 1103 92-93 0.2500290744364193 1103 94-95 0.32128210185774364 1103 96-97 0.36404214827379633 1103 98-99 0.3199952230632981 1103 100-101 0.3644924931670275 1103 102-103 0.25023559963268127 1103 104-105 0.34177285278217084 1103 106-107 0.28558155690340215 1103 108-109 0.26477522127857966 1103 110-111 0.279562597282073 1103 112-113 0.2917840722936518 1103 114-115 0.3262218686134837 1103 116-117 0.2479575781659662 1103 118-119 0.05291590884681341 1103 120-121 0.1623657943614134 1103 122-123 0.2437473788439668 1103 124-125 0.081495323529019 1104 1 0.31093645783453283 1104 2 0.36708809670972187 1104 3 0.24895651179237177 1104 4 0.4577189373568302 1104 5 0.27516890962279206 1104 6 0.3624731494671991 1104 7 0.407922825239865 1104 8 0.36474081830610317 1104 9 0.4498717176394891 1104 10-11 0.4367329453483322 1104 12-13 0.3996214572942236 1104 14-15 0.48908027113744623 1104 16-17 0.49856301680219417 1104 18-19 0.6598625391057382 1104 20-21 0.6442252107422917 1104 22-23 0.571184646802827 1104 24-25 0.7198882732645515 1104 26-27 0.7561456169635505 1104 28-29 0.49071160382489154 1104 30-31 0.5443798514549911 1104 32-33 0.6251839229826572 1104 34-35 0.6384398839838923 1104 36-37 0.5728576570395063 1104 38-39 0.6010985305026963 1104 40-41 0.5924247906188427 1104 42-43 0.5684609338719184 1104 44-45 0.4714148718688307 1104 46-47 0.4475410462891958 1104 48-49 0.39948892767617394 1104 50-51 0.26682863920286337 1104 52-53 0.33298399236806375 1104 54-55 0.24602423872336487 1104 56-57 0.2620156734814003 1104 58-59 0.27243919647012405 1104 60-61 0.20851708657725254 1104 62-63 0.29897136951458236 1104 64-65 0.27256094066392933 1104 66-67 0.1866304953111353 1104 68-69 0.19603753132400925 1104 70-71 0.21520580562690128 1104 72-73 0.19094040449711258 1104 74-75 0.2904714527438159 1104 76-77 0.306137369636744 1104 78-79 0.3290850466327697 1104 80-81 0.37956472794181195 1104 82-83 0.2523103030701357 1104 84-85 0.33482245151659384 1104 86-87 0.31676479379302336 1104 88-89 0.31488345728318023 1104 90-91 0.3134500469544328 1104 92-93 0.33061893432040534 1104 94-95 0.3013940210212631 1104 96-97 0.3504080209910718 1104 98-99 0.2342123057230623 1104 100-101 0.1812616593754619 1104 102-103 0.265102512160432 1104 104-105 0.283676801581322 1104 106-107 0.28047956038459887 1104 108-109 0.16004056933601873 1104 110-111 0.18028178230218828 1104 112-113 0.3025048697435295 1104 114-115 0.2519331982065296 1104 116-117 0.2610567095211067 1104 118-119 0.12980865332630742 1104 120-121 0.20893007862864366 1104 122-123 0.2796387217498939 1104 124-125 0.1887205592951915 1105 1 0.24973799907730765 1105 2 0.31572298399247245 1105 3 0.27748698638353986 1105 4 0.3712990536805023 1105 5 0.281555495238365 1105 6 0.22823809677188223 1105 7 0.1139814197679172 1105 8 0.09282315416785991 1105 9 0.1548871033233965 1105 10-11 0.16972187539965233 1105 12-13 0.13818408036073748 1105 14-15 0.2538614248022881 1105 16-17 0.2791146461598899 1105 18-19 0.4156935915631621 1105 20-21 0.48849949703136275 1105 22-23 0.3460225772137875 1105 24-25 0.5894761908025572 1105 26-27 0.5051666731371682 1105 28-29 0.4179205940617976 1105 30-31 0.4590270265284673 1105 32-33 0.5471883448948773 1105 34-35 0.531173286744739 1105 36-37 0.4202931036274933 1105 38-39 0.41324928904944613 1105 40-41 0.3640545594523772 1105 42-43 0.3353013622361871 1105 44-45 0.2850412310639783 1105 46-47 0.28806271457712995 1105 48-49 0.2776562250846979 1105 50-51 0.27922929286989984 1105 52-53 0.19289393751927264 1105 54-55 0.2085459581039828 1105 56-57 0.3176861432267515 1105 58-59 0.34756071124050436 1105 60-61 0.33711597746683 1105 62-63 0.3197697845236931 1105 64-65 0.2377584398366679 1105 66-67 0.2566218853954041 1105 68-69 0.40608764207139103 1105 70-71 0.4077085728146983 1105 72-73 0.330125460206105 1105 74-75 0.3121000240925582 1105 76-77 0.3286838130869256 1105 78-79 0.22253205054575176 1105 80-81 0.21673902704397818 1105 82-83 0.2991985190612674 1105 84-85 0.2610365314630769 1105 86-87 0.1526744491529044 1105 88-89 0.0926344690580514 1105 90-91 0.11382603722447016 1105 92-93 0.16484652269378586 1105 94-95 0.1590751475830352 1105 96-97 0.12223158278188606 1105 98-99 0.0435538647125604 1105 100-101 0.07167653197704738 1105 102-103 0.05691379174878364 1105 104-105 0.026746158703623735 1105 106-107 0.058990476057317665 1105 108-109 0.09590348287242634 1105 110-111 0.08088754994782477 1105 112-113 0.11533540049673974 1105 114-115 0.14588833483755081 1105 116-117 0.14615684519613836 1105 118-119 0.10284947837737235 1105 120-121 0.13652535750489392 1105 122-123 0.07890494297882711 1105 124-125 0.045784781602655755 1106 1 0.14768041995090186 1106 2 0.14658203033945227 1106 3 0.21512526970319001 1106 4 0.16275096044178383 1106 5 0.042844841546404666 1106 6 0.04454155640213031 1106 7 0.023018586531094343 1106 8 0.1221937948259324 1106 9 0.10305559964425015 1106 10-11 0.2463277575983014 1106 12-13 0.14078510814149325 1106 14-15 0.2712948581428165 1106 16-17 0.2374841791390594 1106 18-19 0.3380180627927629 1106 20-21 0.38773420995286756 1106 22-23 0.44077734436919513 1106 24-25 0.533512379015157 1106 26-27 0.45659011113842496 1106 28-29 0.4441686495708339 1106 30-31 0.3525912910720521 1106 32-33 0.6135635312734493 1106 34-35 0.610707758962981 1106 36-37 0.4563840664607284 1106 38-39 0.5211198621169117 1106 40-41 0.36222608221831365 1106 42-43 0.1908823287213366 1106 44-45 0.18619158283777892 1106 46-47 0.17916244401293824 1106 48-49 0.06399868550384014 1106 50-51 0.06262308785044013 1106 52-53 0.07379656467536222 1106 54-55 -0.020455254491679398 1106 56-57 0.05438885149156647 1106 58-59 0.005907041474081609 1106 60-61 0.0719826423848886 1106 62-63 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-1.6723858891261365 2216 80-81 -1.5700423716391612 2216 82-83 -1.6052855688643604 2216 84-85 -1.6607382214955493 2216 86-87 -1.6032187894159335 2216 88-89 -1.5887752456595443 2216 90-91 -1.6021729510117204 2216 92-93 -1.6077981186530792 2216 94-95 -1.5295961323553051 2216 96-97 -1.5520792736151314 2216 98-99 -1.4934127324444333 2216 100-101 -1.4513474315451873 2216 102-103 -1.5496910909219572 2216 104-105 -1.529907884121048 2216 106-107 -1.5641607969122013 2216 108-109 -1.452101389587856 2216 110-111 -1.5351682562876263 2216 112-113 -1.6491710397690333 2216 114-115 -1.6041599506440676 2216 116-117 -1.530066598235237 2216 118-119 -1.3741800202140482 2216 120-121 -1.3593461891906458 2216 122-123 -1.3957072469543021 2216 124-125 -1.1739716382243444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32040.0 3 6574.0 4 5924.0 5 5490.0 6 6865.0 7 6481.0 8 5739.0 9 5627.0 10 6087.0 11 6547.0 12 6445.0 13 6029.0 14 5706.0 15 5580.0 16 5068.0 17 4626.0 18 4267.0 19 4203.0 20 4087.0 21 4136.0 22 4850.0 23 7047.0 24 11874.0 25 15096.0 26 17826.0 27 16788.0 28 19013.0 29 22368.0 30 26362.0 31 32377.0 32 42670.0 33 56785.0 34 83782.0 35 138288.0 36 270694.0 37 419496.0 38 147293.0 39 378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.73669100289817 18.19861985055299 11.917925951532135 24.14676319501671 2 16.454989124126477 21.16124528581741 37.47383088612005 24.909934703936067 3 18.71403077764617 26.049694080892184 27.971340961864378 27.264934179597272 4 12.691832756738 17.214993519680778 34.52217425765477 35.57099946592645 5 14.031316014164663 39.43525041585735 32.06697259253464 14.466460977443354 6 32.67688850865656 36.75279473030631 16.479897935728342 14.090418825308785 7 29.245327285524358 33.916307663013725 19.472698154715555 17.365666896746358 8 26.770748612044272 35.703579987324105 18.753043166035138 18.772628234596482 9 26.824857973285244 17.880881170868644 17.846403055291475 37.447857800554644 10-11 25.17141820533597 27.897617979759303 25.852228581182608 21.078735233722114 12-13 25.36428853651454 25.447085249672043 26.13298886273699 23.055637351076424 14-15 22.578416217183047 27.00475587785834 23.357834752797046 27.05899315216157 16-17 23.533760137970244 29.69576908481824 24.03741137139212 22.733059405819393 18-19 23.46096249265522 27.948997301473312 25.602544014276074 22.987496191595394 20-21 24.36398927743034 27.334051299803825 25.15009115136363 23.151868271402208 22-23 24.192239651383524 27.068516033460543 25.249495160409424 23.489749154746512 24-25 23.765951275609005 27.46973809798263 25.437781311832495 23.32652931457587 26-27 23.54727758227378 27.72717874355392 25.23533028537538 23.490213388796917 28-29 23.358736989863928 27.673776788774695 25.55005739965941 23.417428821701968 30-31 23.513479784998083 27.34235556110202 25.455903150525483 23.68826150337441 32-33 23.261354030584798 27.679468251285815 25.27560934640581 23.78356837172358 34-35 23.834464371037043 27.657570187535175 25.127163098980958 23.380802342446817 36-37 24.20674150272686 27.385590708711703 24.80125391039329 23.606413878168144 38-39 23.54028122184542 27.41782051199204 25.082127621511297 23.959770644651236 40-41 23.45801762148991 27.45425543237462 25.579485596127093 23.508241350008383 42-43 23.84671750014876 27.556303606797066 25.029896547913534 23.56708234514064 44-45 23.45253525666745 27.582689448909285 25.3425360863161 23.622239208107164 46-47 23.709317923320565 27.859684557174653 24.834194451103304 23.596803068401485 48-49 23.68861926868637 27.653491569154898 24.892185355161825 23.76570380699691 50-51 23.551927414202364 27.810195374913167 24.87904463881024 23.75883257207423 52-53 23.836175195199136 27.529479209417566 24.95995370236565 23.674391893017646 54-55 23.36316618097612 27.4859478446561 25.0479286459945 24.102957328373282 56-57 23.423450993273224 27.891352846188607 24.847522877943838 23.83767328259433 58-59 23.53073394698193 27.113773904870524 25.008747705958744 24.3467444421888 60-61 23.43340689132631 27.439613920858676 25.231819902283153 23.89515928553186 62-63 23.250860945889375 27.52289366234326 25.20144280486598 24.02480258690138 64-65 23.188710835704455 27.471927381140205 25.050902023295922 24.288459759859418 66-67 23.44481387003035 27.688205863371167 24.758560468862953 24.108419797735525 68-69 23.376386223256585 27.216154933274932 25.35103600337876 24.05642284008972 70-71 23.281507411758508 27.258065887209337 25.305931616985756 24.154495084046403 72-73 23.46422416900267 27.39689974987453 24.998469868653526 24.14040621246928 74-75 23.245835496722698 27.758504202840744 24.99654027712465 23.9991200233119 76-77 23.50134613802216 27.36002578709462 25.146871512672874 23.991756562210345 78-79 23.576071658940656 27.447736788112337 25.220924938395356 23.75526661455165 80-81 23.566766793451613 27.8165333645252 25.12912546949307 23.487574372530123 82-83 23.709636232634573 27.517627669482174 25.094518038757922 23.67821805912533 84-85 23.56027672285276 27.23891966005419 25.101846360599367 24.098957256493684 86-87 23.56756425906972 27.031416300840238 25.303230720578885 24.097788719511158 88-89 23.534136109205082 27.12285979893654 25.190566896138066 24.15243719572031 90-91 23.385076662934363 27.43544607922976 25.21463003456998 23.9648472232659 92-93 23.595632841213607 26.781065036445696 25.376791384827563 24.246510737513137 94-95 23.345707741814394 26.88938108463198 25.50669564531441 24.258215528239223 96-97 23.482836415938305 26.840101803319662 25.42376396862934 24.253297812112688 98-99 23.09280839912866 27.207347537075233 25.171844417500903 24.5279996462952 100-101 22.938953038575395 27.228614519801198 25.422319556689864 24.410112884933543 102-103 23.192097964779794 26.83020879947443 25.639075194198064 24.338618041547708 104-105 22.665245202558634 27.474452753321565 25.32059456647045 24.539707477649348 106-107 22.8331812388799 27.6910089219298 25.268404477597215 24.207405361593086 108-109 22.95915470209709 27.73318372308503 24.895757359379118 24.411904215438756 110-111 22.715272951318003 28.099901936915924 25.129107326713278 24.055717785052792 112-113 22.84336513561169 27.943235398639377 25.226546599037942 23.98685286671099 114-115 22.36147396039358 28.296620601011064 25.098033080063498 24.243872358531863 116-117 22.569181860396288 28.238037648811805 25.29859314483413 23.89418734595778 118-119 22.46230001716466 28.269211541342443 24.898760902376743 24.369727539116155 120-121 22.5042850699143 28.344643875371272 25.15134339282219 23.99972766189224 122-123 22.65893217331804 28.208477629563927 25.052330941725693 24.08025925539234 124-125 22.76036084468165 28.385818364655613 24.878614365155006 23.975206425507732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6105.0 1 4952.0 2 3359.5 3 2372.0 4 1470.5 5 950.0 6 752.5 7 725.5 8 809.0 9 840.0 10 692.5 11 564.5 12 530.0 13 541.5 14 530.0 15 551.5 16 627.0 17 693.5 18 784.0 19 958.5 20 1216.0 21 1515.5 22 1935.0 23 2538.5 24 3278.0 25 4425.0 26 6023.5 27 7651.0 28 9205.0 29 10859.5 30 12591.5 31 15410.5 32 19508.0 33 21494.5 34 22394.0 35 23626.5 36 25333.5 37 27817.5 38 29231.0 39 29965.0 40 31479.5 41 35565.5 42 39056.0 43 41837.0 44 44685.0 45 47701.0 46 49300.0 47 49157.0 48 51088.5 49 52667.5 50 53157.5 51 53081.0 52 54443.0 53 57705.0 54 59790.5 55 58030.0 56 53143.5 57 49141.5 58 47040.0 59 43127.5 60 37539.5 61 32418.5 62 28141.5 63 22491.0 64 17179.5 65 14143.5 66 10800.5 67 8842.0 68 6595.0 69 4091.0 70 3176.0 71 2652.0 72 2264.0 73 2004.0 74 1601.0 75 1301.5 76 944.0 77 513.0 78 300.5 79 196.0 80 103.5 81 56.0 82 45.5 83 35.0 84 20.5 85 13.5 86 12.0 87 12.5 88 10.0 89 6.0 90 3.0 91 3.0 92 5.0 93 5.5 94 3.0 95 2.0 96 1.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0650795507402884 2 0.05039074931928286 3 0.04590250444064228 4 0.045766497020077415 5 0.044406422814428755 6 0.0042162300375108465 7 2.0401113084729901E-4 8 0.0 9 6.800371028243301E-4 10-11 0.0037062022103925988 12-13 0.002652144701014887 14-15 0.007956434103044663 16-17 0.0031281706729919183 18-19 0.005168281981464909 20-21 0.0103025621077886 22-23 0.01625288675750149 24-25 0.003638198500110166 26-27 0.015912868206089326 28-29 0.007888430392762229 30-31 0.006970380303949384 32-33 0.002652144701014887 34-35 0.017034929425749468 36-37 0.015470844089253508 38-39 0.02635143773444279 40-41 0.005950324649712888 42-43 0.0013940760607898768 44-45 4.0802226169459803E-4 46-47 0.002414131715026372 48-49 0.003026165107568269 50-51 0.001870102032766908 52-53 0.0015300834813547427 54-55 0.006324345056266271 56-57 0.0028221539767209697 58-59 0.008330454509598044 60-61 0.016286888612642707 62-63 0.012070658575131859 64-65 0.0024481335701675884 66-67 0.0038762114860986818 68-69 0.009928541701235218 70-71 0.002754150266438537 72-73 0.0031281706729919183 74-75 9.860537990952786E-4 76-77 3.4001855141216504E-4 78-79 0.002890157687003403 80-81 0.002652144701014887 82-83 0.0014620797710723098 84-85 0.010608578804059549 86-87 0.006324345056266271 88-89 0.0018361001776256913 90-91 8.500463785304127E-4 92-93 0.002210120584179073 94-95 0.0 96-97 0.0017340946122020417 98-99 0.024175319005404934 100-101 0.013974762463039983 102-103 0.006936378448808167 104-105 0.013124716084509571 106-107 0.0325397753701442 108-109 0.0013940760607898768 110-111 0.0017680964673432583 112-113 0.015334836668688644 114-115 0.031859738267319866 116-117 0.05035674746414165 118-119 0.35963762182864695 120-121 0.11870047629798683 122-123 0.02023110380902382 124-125 0.32131753108449596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1470508.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.77996270010312 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44184206215628 51.30577686251914 2 10.477183849360797 13.364687902287088 3 3.7868953379463095 7.245841302102302 4 1.7891696520864249 4.5645269469691465 5 1.0024275248186523 3.196739507124517 6 0.6151614378887739 2.3540984137852683 7 0.4037733339378292 1.8026853724495715 8 0.2826324901451965 1.4421031743438315 9 0.20745510264290928 1.1908330837659737 >10 0.950791406219096 10.123767829674224 >50 0.03426368550214943 1.5179086882650201 >100 0.00818862710879889 0.8250496838577691 >500 0.0 0.0 >1k 0.0 0.0 >5k 2.154901867140908E-4 1.0659812328561316 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7756 0.5274367769505504 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3600742056486603E-4 0.0 20-21 0.0 0.0 0.0 2.0401113084729904E-4 0.0 22-23 0.0 0.0 0.0 8.500463785304127E-4 0.0 24-25 0.0 0.0 0.0 0.002380129859885155 0.0 26-27 0.0 0.0 0.0 0.004692256009487878 0.0 28-29 0.0 0.0 0.0 0.009350510163834539 0.0 30-31 0.0 0.0 0.0 0.02036711122958869 0.0 32-33 0.0 0.0 0.0 0.04056421318347129 0.0 34-35 0.0 0.0 0.0 0.06909176964695193 0.0 36-37 0.0 0.0 0.0 0.1100300032369766 0.0 38-39 0.0 0.0 0.0 0.16973726086495278 0.0 40-41 0.0 0.0 0.0 0.23716293960998514 0.0 42-43 0.0 0.0 0.0 0.3005763994483539 0.0 44-45 0.0 0.0 0.0 0.3759245104412897 0.0 46-47 0.0 0.0 0.0 0.46487336349071207 0.0 48-49 0.0 0.0 0.0 0.56851101796114 0.0 50-51 0.0 0.0 0.0 0.6868714757077146 0.0 52-53 0.0 0.0 0.0 0.7955074028839013 0.0 54-55 0.0 0.0 0.0 0.9087335805041523 0.0 56-57 0.0 0.0 0.0 1.0494672589336473 0.0 58-59 0.0 3.400185514121651E-5 0.0 1.2045157183775947 0.0 60-61 0.0 6.800371028243301E-5 0.0 1.3838075005372295 0.0 62-63 0.0 6.800371028243301E-5 0.0 1.5564349190891855 0.0 64-65 0.0 6.800371028243301E-5 0.0 1.7293003506271303 0.0 66-67 0.0 6.800371028243301E-5 0.0 1.9294352699883306 0.0 68-69 0.0 6.800371028243301E-5 0.0 2.165408144668373 0.0 70-71 0.0 6.800371028243301E-5 0.0 2.4390210729897426 0.0 72-73 0.0 6.800371028243301E-5 0.0 2.7093018195072727 0.0 74-75 0.0 6.800371028243301E-5 0.0 2.98566889809508 0.0 76-77 0.0 6.800371028243301E-5 0.0 3.284851221482644 0.0 78-79 0.0 6.800371028243301E-5 0.0 3.6239857246611376 0.0 80-81 0.0 6.800371028243301E-5 0.0 4.004364478125927 0.0 82-83 0.0 6.800371028243301E-5 0.0 4.4098706025400745 0.0 84-85 0.0 6.800371028243301E-5 0.0 4.807862316968014 0.0 86-87 0.0 6.800371028243301E-5 0.0 5.21415048405041 0.0 88-89 0.0 6.800371028243301E-5 0.0 5.651074322615042 0.0 90-91 0.0 1.0200556542364952E-4 0.0 6.147501407676803 0.0 92-93 0.0 1.3600742056486603E-4 0.0 6.687280858043614 0.0 94-95 0.0 1.3600742056486603E-4 0.0 7.217743800101734 0.0 96-97 0.0 1.3600742056486603E-4 0.0 7.736340094715567 0.0 98-99 0.0 1.3600742056486603E-4 0.0 8.29036632238655 0.0 100-101 0.0 1.3600742056486603E-4 0.0 8.921440753807527 0.0 102-103 0.0 1.3600742056486603E-4 0.0 9.595833548678415 0.0 104-105 0.0 1.3600742056486603E-4 0.0 10.260467811123775 0.0 106-107 0.0 1.3600742056486603E-4 0.0 10.901368778680565 0.0 108-109 0.0 1.3600742056486603E-4 0.0 11.564166940948297 0.0 110-111 0.0 1.3600742056486603E-4 0.0 12.290548572330106 0.0 112-113 0.0 1.3600742056486603E-4 0.0 13.058004444722503 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3445 0.0 43.015854 1 GGTATCA 2345 0.0 40.86029 1 GTACATG 8065 0.0 37.26537 1 TACATGG 8160 0.0 36.607735 2 ATCAACG 4020 0.0 35.08178 3 TCAACGC 4050 0.0 34.673805 4 ACATGGG 8240 0.0 34.59136 3 CAACGCA 4170 0.0 33.817543 5 AACGCAG 4210 0.0 33.619267 6 TATCAAC 4630 0.0 31.230907 2 CATGGGG 4185 0.0 30.995993 4 ACGCAGA 4735 0.0 29.51289 7 CGCAGAG 4850 0.0 28.813099 8 GAACAAA 1730 0.0 28.552912 1 CATGGGA 4470 0.0 28.087912 4 ATGGGAG 1545 0.0 25.418224 5 GTATAGG 260 1.1023076E-9 25.178959 1 CCAAGAC 1305 0.0 24.62309 3 GCAGAGT 5450 0.0 24.225046 9 ATGGGAT 1530 0.0 22.94512 5 >>END_MODULE