FastQCFastQC Report
Thu 26 May 2016
SRR1364182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470508
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98840.672148672431568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87060.5920403017188618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54690.3719122915346261No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.14450788435017015No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.1361434279854309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.073.824971
GTATCAA75100.052.7998771
ATCAACG98300.040.1335453
TCAACGC99950.039.351944
CAACGCA100400.039.16895
TATCAAC100750.039.158922
AACGCAG102950.038.4876636
ACGCAGA116850.033.80757
GTGGTAT15450.033.5265921
CGCAGAG119300.033.0633438
GTACATG76250.031.4676151
TGGTATC16850.031.4305782
ACATGGG75600.030.6178933
GTAAGGT6650.030.4231914
TACATGG78550.030.3023322
GCAGAGT130400.030.2032769
GAGTACT90150.028.31165112-13
CATGGGG36550.028.1647724
TAAGGTG7200.027.2681145
CAGAGTA127300.027.01301810-11
Produced by FastQC (version 0.11.5)