##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470508 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.340028071931606 33.0 31.0 34.0 30.0 34.0 2 32.303913341511915 34.0 31.0 34.0 30.0 34.0 3 32.44670617228876 34.0 31.0 34.0 30.0 34.0 4 36.037423121805524 37.0 35.0 37.0 35.0 37.0 5 35.98301131309724 37.0 35.0 37.0 35.0 37.0 6 36.05069744605266 37.0 35.0 37.0 35.0 37.0 7 35.98955871032324 37.0 35.0 37.0 35.0 37.0 8 36.000094525157294 37.0 35.0 37.0 35.0 37.0 9 37.708109034428915 39.0 38.0 39.0 35.0 39.0 10-11 37.68412990612768 39.0 37.5 39.0 35.0 39.0 12-13 37.64476561841214 39.0 37.0 39.0 35.0 39.0 14-15 39.0192746316239 41.0 38.0 41.0 35.0 41.0 16-17 39.04622552206449 41.0 38.0 41.0 35.5 41.0 18-19 39.10725885204297 41.0 38.5 41.0 35.0 41.0 20-21 39.13180683138072 41.0 39.0 41.0 35.0 41.0 22-23 39.08048035100795 41.0 39.0 41.0 35.0 41.0 24-25 39.02496042184062 41.0 39.0 41.0 35.0 41.0 26-27 38.90562955114831 41.0 38.0 41.0 35.0 41.0 28-29 38.78976788973607 40.5 38.0 41.0 35.0 41.0 30-31 38.59326062829988 40.0 38.0 41.0 34.5 41.0 32-33 38.36243053420995 40.0 38.0 41.0 34.0 41.0 34-35 38.22802426100368 40.0 38.0 41.0 34.0 41.0 36-37 38.077639836029455 40.0 37.0 41.0 33.5 41.0 38-39 37.932261504187665 40.0 37.0 41.0 33.0 41.0 40-41 37.75030533665917 40.0 37.0 41.0 33.0 41.0 42-43 37.55490041536666 40.0 37.0 41.0 33.0 41.0 44-45 37.287200409654346 40.0 36.0 41.0 32.5 41.0 46-47 36.91167610104807 40.0 36.0 41.0 31.5 41.0 48-49 36.535984843333054 40.0 35.5 41.0 30.5 41.0 50-51 36.284595867550536 40.0 35.0 41.0 30.0 41.0 52-53 36.10128098589059 39.0 35.0 41.0 30.0 41.0 54-55 35.878386584772066 39.0 35.0 41.0 29.0 41.0 56-57 35.6518359641702 39.0 35.0 41.0 29.0 41.0 58-59 35.408734940578356 38.0 35.0 41.0 28.5 41.0 60-61 35.1452739461465 38.0 35.0 40.5 28.0 41.0 62-63 34.872574307654226 37.0 35.0 40.0 28.0 41.0 64-65 34.56710436121395 36.5 34.0 40.0 28.0 41.0 66-67 34.2520958743509 36.0 34.0 39.0 27.5 41.0 68-69 33.91473150775106 35.5 34.0 39.0 26.5 41.0 70-71 33.57084728542789 35.0 34.0 38.5 26.0 40.5 72-73 33.24721252791552 35.0 34.0 37.5 26.0 40.0 74-75 33.14563776599651 35.0 34.0 37.0 26.0 39.0 76-77 32.89960374238018 35.0 34.0 36.5 26.0 39.0 78-79 32.629290014063166 35.0 34.0 36.0 26.0 39.0 80-81 32.380076816991135 35.0 34.0 36.0 26.0 37.0 82-83 32.14976933141472 35.0 33.0 35.5 25.5 37.0 84-85 31.959263397410965 35.0 33.0 35.0 25.0 36.5 86-87 31.80461003952376 35.0 33.0 35.0 25.0 36.0 88-89 31.668440430109868 35.0 33.0 35.0 25.0 36.0 90-91 31.560842239552592 35.0 33.0 35.0 25.0 36.0 92-93 31.462240259828576 35.0 33.0 35.0 24.0 35.5 94-95 31.378973116773253 35.0 33.0 35.0 24.0 35.0 96-97 31.303495118693675 35.0 33.0 35.0 24.0 35.0 98-99 31.23571412056242 35.0 33.0 35.0 24.0 35.0 100-101 31.165119128899672 35.0 33.0 35.0 23.0 35.0 102-103 30.23232515566049 34.0 30.5 35.0 19.5 35.0 104-105 30.9402754014259 35.0 32.5 35.0 21.5 35.0 106-107 31.044696458638782 35.0 33.0 35.0 23.0 35.0 108-109 31.03018412684596 35.0 33.0 35.0 20.0 35.0 110-111 30.96865810998648 35.0 33.0 35.0 20.0 35.0 112-113 31.095879451182856 35.0 33.0 35.0 23.0 35.0 114-115 31.07246475367696 35.0 33.0 35.0 20.0 35.0 116-117 31.024743830023365 35.0 33.0 35.0 19.5 35.0 118-119 30.924118059881344 35.0 33.0 35.0 19.0 35.0 120-121 30.82050488674662 35.0 33.0 35.0 16.5 35.0 122-123 30.674295549565183 35.0 33.0 35.0 2.0 35.0 124-125 29.969104894363035 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27006722684230056 1101 2 0.31517614692855744 1101 3 0.25788008971643706 1101 4 0.14762695112441548 1101 5 0.16467995682316428 1101 6 0.16081358868841278 1101 7 0.25370536605102245 1101 8 0.19748805066118535 1101 9 0.30182988305379865 1101 10-11 0.18561178483281537 1101 12-13 0.25136137627468713 1101 14-15 0.39250840029453826 1101 16-17 0.30288070969956493 1101 18-19 0.3501057779200565 1101 20-21 0.24143835910287237 1101 22-23 0.18888870708727978 1101 24-25 0.20345099587562032 1101 26-27 0.279927024549238 1101 28-29 0.24765662808288624 1101 30-31 0.298981924914294 1101 32-33 0.3798107995747486 1101 34-35 0.3095763436121217 1101 36-37 0.22255564637966074 1101 38-39 0.2737349927805681 1101 40-41 0.25622346123245165 1101 42-43 0.21595539311353917 1101 44-45 0.20865351286471423 1101 46-47 0.17371716810746562 1101 48-49 0.052073772763037596 1101 50-51 0.10089396807224915 1101 52-53 0.15729973857756363 1101 54-55 0.039201333436189145 1101 56-57 0.09107292260636513 1101 58-59 0.08253146515894372 1101 60-61 0.10839271650926463 1101 62-63 0.09706897878205467 1101 64-65 0.08245698136857982 1101 66-67 0.04040221933761501 1101 68-69 0.015555480928014731 1101 70-71 0.06543784228866656 1101 72-73 0.11548657746990187 1101 74-75 0.06684371850266757 1101 76-77 0.11491164033682821 1101 78-79 0.058442759939346445 1101 80-81 0.03819631706463866 1101 82-83 0.08226286269281502 1101 84-85 0.09482982890981262 1101 86-87 -0.12345479773383516 1101 88-89 0.034312695895781786 1101 90-91 0.1460147134389338 1101 92-93 0.08869756309469423 1101 94-95 0.08676680635316458 1101 96-97 0.12491127011655223 1101 98-99 0.15442367092778753 1101 100-101 0.21523730399324847 1101 102-103 0.1633353555804682 1101 104-105 0.22502195896542077 1101 106-107 0.20919587979739163 1101 108-109 0.2865674626788106 1101 110-111 0.18491986351995848 1101 112-113 0.11326412120765639 1101 114-115 0.18875891207074602 1101 116-117 0.1920168751822402 1101 118-119 0.2053383614798392 1101 120-121 0.27983202179940747 1101 122-123 0.24653128489594422 1101 124-125 0.2820364416510657 1102 1 0.18010070633485498 1102 2 0.353677897829094 1102 3 0.2525039722004081 1102 4 0.16387905404459246 1102 5 0.21049769593731327 1102 6 0.21578751749515845 1102 7 0.25187586730692146 1102 8 0.26265165199769314 1102 9 0.34131093419614444 1102 10-11 0.24385038223940114 1102 12-13 0.3475097295585954 1102 14-15 0.3846750017414138 1102 16-17 0.3835612698539492 1102 18-19 0.40445526903845774 1102 20-21 0.43025500453128984 1102 22-23 0.387329452398987 1102 24-25 0.39328707680166985 1102 26-27 0.4783905131725703 1102 28-29 0.4117409254985134 1102 30-31 0.39921437493754297 1102 32-33 0.4412665721951541 1102 34-35 0.4848152319993204 1102 36-37 0.3886119226975211 1102 38-39 0.45234591157690573 1102 40-41 0.48321809983119124 1102 42-43 0.40861565820690515 1102 44-45 0.44826232790472886 1102 46-47 0.23747536656961898 1102 48-49 0.15372132501411784 1102 50-51 0.17161864356198464 1102 52-53 0.10154484458480795 1102 54-55 0.07942622465292715 1102 56-57 0.18242210267653292 1102 58-59 0.14867537011866006 1102 60-61 0.06253015969279119 1102 62-63 0.1805335878479397 1102 64-65 0.17230644071324264 1102 66-67 0.001963012544045739 1102 68-69 0.10288812527279134 1102 70-71 0.1464277783732726 1102 72-73 0.140342341394323 1102 74-75 0.030404585290412456 1102 76-77 0.0903321407565727 1102 78-79 0.05953276659510465 1102 80-81 0.07003829118409044 1102 82-83 0.04001816472733566 1102 84-85 0.13229642467085867 1102 86-87 0.14600762396060674 1102 88-89 0.09222184974425218 1102 90-91 0.02884485100252121 1102 92-93 0.06934634950489738 1102 94-95 0.09585610395273747 1102 96-97 0.13961649338563475 1102 98-99 0.10840692783690287 1102 100-101 0.0051623514030190165 1102 102-103 -0.019397453987775037 1102 104-105 0.03046563749540354 1102 106-107 0.12540416839664204 1102 108-109 0.1278763402629366 1102 110-111 0.05893797120954147 1102 112-113 0.11258550092397002 1102 114-115 0.15745374699778125 1102 116-117 0.13247822994780023 1102 118-119 0.09794377505692609 1102 120-121 0.14522773869869354 1102 122-123 0.1747460352713759 1102 124-125 0.18819051420891597 1103 1 0.13918587687639672 1103 2 0.21183753341569656 1103 3 0.139061749892889 1103 4 0.09130943632891331 1103 5 0.07062675901444493 1103 6 0.0896059676292964 1103 7 0.09298220146342118 1103 8 0.17780398140283182 1103 9 0.26809173595679425 1103 10-11 0.2354676799332438 1103 12-13 0.21975125153044672 1103 14-15 0.2379822964070044 1103 16-17 0.2633107143197222 1103 18-19 0.18380432367060706 1103 20-21 0.20657762383787315 1103 22-23 0.24731499358102127 1103 24-25 0.1783019408077493 1103 26-27 0.17242091934166126 1103 28-29 0.24678649849848 1103 30-31 0.27811036528126465 1103 32-33 0.232473990123232 1103 34-35 0.23360116045589763 1103 36-37 0.34184095208005516 1103 38-39 0.2883704403857834 1103 40-41 0.348068883976822 1103 42-43 0.41183850313928616 1103 44-45 0.39610043606009526 1103 46-47 0.35012576599979184 1103 48-49 0.28673925138930656 1103 50-51 0.24469250722258096 1103 52-53 0.26296603123151385 1103 54-55 0.15913291110982897 1103 56-57 0.28544843137365916 1103 58-59 0.28166738578024564 1103 60-61 0.28272004363838477 1103 62-63 0.25332819159528697 1103 64-65 0.30791074679660824 1103 66-67 0.2873694162223046 1103 68-69 0.32943850295076516 1103 70-71 0.3081566947296466 1103 72-73 0.32334139453367783 1103 74-75 0.3049264633159936 1103 76-77 0.22763962308823693 1103 78-79 0.21303156595875095 1103 80-81 0.2271436912753586 1103 82-83 0.28128987961138563 1103 84-85 0.2819274902302915 1103 86-87 0.35516487533504915 1103 88-89 0.28553208735509017 1103 90-91 0.3898319532250838 1103 92-93 0.3266714042052925 1103 94-95 0.3258643356691593 1103 96-97 0.2939232165227317 1103 98-99 0.34103896584781523 1103 100-101 0.3253202468133054 1103 102-103 0.2785978245044127 1103 104-105 0.2869304138528861 1103 106-107 0.2868062685506132 1103 108-109 0.17876764748509544 1103 110-111 0.14873853335473441 1103 112-113 0.2515239720425839 1103 114-115 0.32927493961567933 1103 116-117 0.3706002470168812 1103 118-119 0.367052659765978 1103 120-121 0.31797691872590406 1103 122-123 0.44153436500067045 1103 124-125 0.42722377802050104 1104 1 0.2100114409167375 1104 2 0.29462029778182597 1104 3 0.23149924756726392 1104 4 0.09738870999132132 1104 5 0.1186192839554252 1104 6 0.177060023717047 1104 7 0.14177039847753292 1104 8 0.11362803637427987 1104 9 0.2030533477250529 1104 10-11 0.23260499693279968 1104 12-13 0.22874829954364628 1104 14-15 0.2687913847226824 1104 16-17 0.2514507765843206 1104 18-19 0.24212218732520085 1104 20-21 0.27276817582632873 1104 22-23 0.16326543823953443 1104 24-25 0.24928936799000212 1104 26-27 0.2478823223209332 1104 28-29 0.20723214927927103 1104 30-31 0.19348395285480535 1104 32-33 0.3603756401763576 1104 34-35 0.3707614649157165 1104 36-37 0.3954909349221509 1104 38-39 0.3827708645788448 1104 40-41 0.3986270327420769 1104 42-43 0.4276480669150189 1104 44-45 0.4652010103331605 1104 46-47 0.5852134780446434 1104 48-49 0.6584776987434822 1104 50-51 0.6887663110270879 1104 52-53 0.6499291231276061 1104 54-55 0.6395685565572009 1104 56-57 0.6327511257851413 1104 58-59 0.6535429930254324 1104 60-61 0.5861731239994015 1104 62-63 0.6043431954609915 1104 64-65 0.5755788425997395 1104 66-67 0.5884208031186233 1104 68-69 0.6319759092541233 1104 70-71 0.6038052015783393 1104 72-73 0.6133307897071418 1104 74-75 0.6735666682186121 1104 76-77 0.6116365677405469 1104 78-79 0.6185242666132282 1104 80-81 0.5486292145501608 1104 82-83 0.5287692177709857 1104 84-85 0.5504687787684013 1104 86-87 0.6434974989236579 1104 88-89 0.5666326533592816 1104 90-91 0.46644066199959866 1104 92-93 0.5267963675121763 1104 94-95 0.5061670590216885 1104 96-97 0.4714128700983977 1104 98-99 0.5529201959250045 1104 100-101 0.5153674207200538 1104 102-103 0.5056420960375867 1104 104-105 0.528163240442268 1104 106-107 0.48612501697435917 1104 108-109 0.5245932115254313 1104 110-111 0.5103922661296423 1104 112-113 0.5168952856767142 1104 114-115 0.42255100412941005 1104 116-117 0.4049092381733246 1104 118-119 0.40755358794670116 1104 120-121 0.4385540032048212 1104 122-123 0.4661432474214635 1104 124-125 0.5703413903014294 1105 1 0.1543868747555024 1105 2 0.23371083202370357 1105 3 0.18655405045927154 1105 4 0.12938730015255118 1105 5 0.09675201396930078 1105 6 0.08213081303117065 1105 7 0.11358539825200609 1105 8 0.1823155650889703 1105 9 0.18322000567668795 1105 10-11 0.16008920909358437 1105 12-13 0.11298814217818176 1105 14-15 0.1692182281806751 1105 16-17 0.17977023439513573 1105 18-19 0.20320852158872071 1105 20-21 0.11922318173192537 1105 22-23 0.15342131483633636 1105 24-25 0.17876350052203804 1105 26-27 0.18942610376240054 1105 28-29 0.12940731256630045 1105 30-31 0.09229459099046977 1105 32-33 0.06268989930648416 1105 34-35 0.10105795017806685 1105 36-37 0.08214149408767923 1105 38-39 0.20420262930021948 1105 40-41 0.1529302941717674 1105 42-43 0.00469184416527213 1105 44-45 0.048965072297413315 1105 46-47 0.10226007337580967 1105 48-49 0.19651570371782867 1105 50-51 0.06657007200752219 1105 52-53 0.07600509316014836 1105 54-55 0.04428499016005816 1105 56-57 0.09028871383519999 1105 58-59 0.12993308327985886 1105 60-61 0.1041216249679664 1105 62-63 0.2118333231966929 1105 64-65 0.11278719018646655 1105 66-67 0.1058220535322576 1105 68-69 0.09567455661520796 1105 70-71 0.16382014618868368 1105 72-73 0.16522489133607365 1105 74-75 0.20019892981635223 1105 76-77 0.19804637258644675 1105 78-79 0.19889365874200138 1105 80-81 0.24187033090936438 1105 82-83 0.16163235204376036 1105 84-85 0.1370143983490273 1105 86-87 0.18272031532009692 1105 88-89 0.1061805868797201 1105 90-91 0.10107617357109433 1105 92-93 0.030238537578171076 1105 94-95 0.11226091947719041 1105 96-97 0.200787292062504 1105 98-99 0.12816425330687764 1105 100-101 0.04207372679477572 1105 102-103 0.08113213675606445 1105 104-105 -0.00266600580890497 1105 106-107 0.05787651013811512 1105 108-109 0.026763153778723847 1105 110-111 0.15051854489372474 1105 112-113 0.23412711194881197 1105 114-115 0.12287953408509367 1105 116-117 0.12049542095500954 1105 118-119 0.2402781333116195 1105 120-121 0.15173294216143773 1105 122-123 0.08157609227413687 1105 124-125 0.09713341632995665 1106 1 0.016522818633099234 1106 2 0.1335643520819758 1106 3 0.06033716733453076 1106 4 0.03795213972069433 1106 5 0.058798375072619535 1106 6 0.07261990673192997 1106 7 0.07137032292379075 1106 8 0.06898911859954637 1106 9 0.13290333864012638 1106 10-11 0.15552159730174253 1106 12-13 0.11948218714307757 1106 14-15 0.21069394297656885 1106 16-17 0.15363536793197596 1106 18-19 0.11001903182718564 1106 20-21 0.12081922798407163 1106 22-23 0.09583331085757152 1106 24-25 0.06915897618841171 1106 26-27 0.09434992813980614 1106 28-29 0.1168634038923031 1106 30-31 0.1602575513379847 1106 32-33 0.22536233101474323 1106 34-35 0.13192930406836467 1106 36-37 0.21472500883957935 1106 38-39 0.22391880928089591 1106 40-41 0.1933270812976744 1106 42-43 0.17344379874439397 1106 44-45 0.17858408321480823 1106 46-47 0.29637767296098616 1106 48-49 0.2587889473204257 1106 50-51 0.2344769422396098 1106 52-53 0.09220575453927893 1106 54-55 0.0875868619154545 1106 56-57 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44-45 -0.6624100232471477 2216 46-47 -0.6624927031732639 2216 48-49 -0.6519313423709718 2216 50-51 -0.7220513516091245 2216 52-53 -0.6361596172751192 2216 54-55 -0.6024179950429911 2216 56-57 -0.6229728178266853 2216 58-59 -0.5701677539421937 2216 60-61 -0.5582345137500582 2216 62-63 -0.7646020229896848 2216 64-65 -0.684533295817161 2216 66-67 -0.6784999986703255 2216 68-69 -0.7159579781958598 2216 70-71 -0.5610207973292631 2216 72-73 -0.49241810241376527 2216 74-75 -0.525370490782862 2216 76-77 -0.5687735759399715 2216 78-79 -0.4983619051556687 2216 80-81 -0.5561396289306799 2216 82-83 -0.6155243488922082 2216 84-85 -0.5018876701260275 2216 86-87 -0.5679602332886553 2216 88-89 -0.56771247961073 2216 90-91 -0.6116210159461204 2216 92-93 -0.47831040247964296 2216 94-95 -0.5227428773146556 2216 96-97 -0.532369821575994 2216 98-99 -0.6566976859166047 2216 100-101 -0.7489816956558855 2216 102-103 -0.8108079899607503 2216 104-105 -0.8984943187016832 2216 106-107 -0.8238213823300278 2216 108-109 -0.7878249701705542 2216 110-111 -0.8212963001875266 2216 112-113 -0.7459693626219632 2216 114-115 -0.666349697534919 2216 116-117 -0.6763449770530201 2216 118-119 -0.6920959522541814 2216 120-121 -0.9135185939594948 2216 122-123 -1.0251174179072287 2216 124-125 -0.9953418491866408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44.0 3 10.0 4 40.0 5 143.0 6 533.0 7 1148.0 8 1631.0 9 2636.0 10 4492.0 11 6095.0 12 7369.0 13 10754.0 14 16443.0 15 15202.0 16 6278.0 17 5249.0 18 5152.0 19 4579.0 20 4262.0 21 4193.0 22 4487.0 23 4960.0 24 5870.0 25 7611.0 26 9752.0 27 12565.0 28 16204.0 29 20647.0 30 26023.0 31 32692.0 32 41392.0 33 54410.0 34 75353.0 35 127649.0 36 265392.0 37 477198.0 38 191245.0 39 805.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.46214090689825 17.667213914472857 12.887855929360809 23.982789249268087 2 17.078629525856208 20.347038981529337 37.55998204619026 25.014349446424198 3 19.20718230917061 24.61472340738061 29.807439908276223 26.37065437517256 4 13.194591348034198 16.441306168091565 35.22908684275145 35.135015641122784 5 13.710656922790251 37.482573504109595 33.195485500475364 15.611284072624795 6 32.92387018501668 35.85662193242668 16.25366721479372 14.965840667762919 7 28.506543317003374 33.07265244391734 20.401929129253293 18.01887510982599 8 27.651192649070932 33.08754525646919 19.172490051057185 20.088772043402688 9 27.693422953156325 14.423178928642347 19.08734940578358 38.79604871241775 10-11 25.888366469274565 25.43212957698972 26.679963658817226 21.99954029491849 12-13 26.81926925933079 23.165259896579958 27.584651018559576 22.43081982552968 14-15 23.37824751718454 24.33815388967622 25.99537030740397 26.288228285735272 16-17 22.82622739896689 27.478497226808695 26.51498665767204 23.180288716552376 18-19 22.621865757763903 24.55417952235002 30.14554322331793 22.678411496568156 20-21 23.25927502604542 24.11085148805719 30.259202942112523 22.37067054378487 22-23 22.922894673133364 23.938836102897774 30.43356445527668 22.70470476869218 24-25 22.740006426352807 24.40126962970267 30.373255491726496 22.485468452218026 26-27 22.567143047068733 24.668063245219223 30.238113129663173 22.52668057804887 28-29 22.310612023816283 24.52386303630756 30.633288598180286 22.53223634169587 30-31 22.549815318735416 24.133929363794042 30.574057306105075 22.74219801136547 32-33 22.42966376245488 24.493406360251015 30.265221270472516 22.811708606821586 34-35 22.869001732734535 24.48691200591904 29.95930657976699 22.684779681579425 36-37 23.450399453794198 24.186981641718372 29.55159033476866 22.811028569718765 38-39 22.808920454700008 24.18687963615295 29.76304787189189 23.24115203725515 40-41 22.76006752770727 24.256081658883073 30.104062713042946 22.87978810036671 42-43 23.293219803577337 24.387339534829948 29.350100972388415 22.9693396892043 44-45 22.93003506271302 24.431556985749143 29.376310771515694 23.262097180022142 46-47 23.3286388105335 24.761646995460072 28.51534979748495 23.394364396521475 48-49 23.516576780776013 24.84400454138814 28.036917310080987 23.60250136775485 50-51 23.46114194705768 25.118598632035503 27.683566065014354 23.73669335589246 52-53 23.70296744875305 24.93908988989084 27.57635739009232 23.78158527126379 54-55 23.278928098317046 24.975552666153465 27.57343040636297 24.17208882916652 56-57 23.37661542813776 25.322779610855566 27.319470550313223 23.981134410693446 58-59 23.389292928812093 24.64278197383127 27.614739385465647 24.353185711890994 60-61 23.550228900488808 24.84481553313549 27.876828959788046 23.728126606587654 62-63 23.286005587931374 24.878195404866606 27.947273375788555 23.88852563141346 64-65 23.191645336169543 24.849031763173 27.905050499555255 24.054272401102203 66-67 23.406906789991435 25.128248895514087 27.591865380057744 23.872978934436738 68-69 23.316985390903753 24.75126996071529 28.01016988689282 23.92157476148813 70-71 23.284243214403034 24.887765352223983 27.840687669729185 23.987303763643794 72-73 23.33336506839813 25.10326363406388 27.448609596139566 24.11476170139843 74-75 23.108926680074738 25.42285571477874 27.332876574923965 24.13534103022256 76-77 23.222818430930253 25.150672001376407 27.528203928539735 24.098305639153605 78-79 23.196647119987325 25.269347710331946 27.484687149990783 24.04931801968994 80-81 23.091125404749995 25.64058710383123 27.361184734647555 23.90710275677122 82-83 23.23864115488506 25.437270993768042 27.235352441781075 24.08873540956583 84-85 23.108532218961773 25.13079018147074 27.064675940251004 24.696001659316483 86-87 23.11949339955988 24.926045965067853 27.261259374311464 24.693201261060803 88-89 22.88331651374899 25.152158301756945 27.191181550865416 24.77334363362865 90-91 22.787025435355844 25.442496306509554 27.18997206718157 24.58050619095303 92-93 22.937051047732393 24.81740978450291 27.373417775321972 24.872121392442725 94-95 22.72257240954609 24.88143560594082 27.349533648416912 25.04645833609618 96-97 22.963241024469262 24.71519852458147 27.369571396900916 24.951989054048358 98-99 22.7469353791683 24.925911480073545 26.986856024965157 25.340297115793003 100-101 22.542279334875186 24.82679678884154 27.28416888813621 25.34675498814706 102-103 22.855359730091894 24.227279220205016 27.44872902396387 25.46863202573922 104-105 22.38481957225645 24.576483286145567 27.30097315840195 25.73772398319603 106-107 22.628685361408778 24.657577111899755 27.245977827799127 25.467759698892344 108-109 22.714439687790904 24.669946932343926 26.90114886390729 25.71446451595788 110-111 22.39772052486697 24.954885954822696 27.12984886709841 25.51754465321192 112-113 22.473759305126542 24.831103367323486 27.137339791325775 25.557797536224196 114-115 21.943397368372732 25.116814247155894 26.95044600567019 25.989342378801183 116-117 22.023815798018965 25.12110721937416 27.181434544637874 25.673642437968997 118-119 21.857140282528466 25.272672309347012 26.780866845461492 26.089320562663033 120-121 21.84401580950257 25.361269710875423 27.031202822425993 25.763511657196013 122-123 21.887235375862247 25.347100027847567 26.78572096466147 25.979943631628714 124-125 21.88957210483275 25.490425527572274 26.597004039879007 26.022998327715964 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 736.0 1 537.0 2 730.0 3 1106.0 4 688.5 5 478.0 6 1193.5 7 2584.0 8 4162.0 9 4756.5 10 3379.5 11 1764.5 12 1469.5 13 1547.0 14 1640.0 15 1782.5 16 1939.5 17 2081.0 18 2223.5 19 2332.0 20 2435.0 21 2647.5 22 2950.0 23 3496.5 24 4173.0 25 5135.0 26 6737.0 27 8381.0 28 9776.5 29 11408.5 30 13253.0 31 15835.5 32 19625.0 33 21432.0 34 21831.0 35 22818.5 36 24501.0 37 26728.0 38 28303.0 39 29299.5 40 30736.5 41 34564.0 42 38062.5 43 40940.0 44 43845.0 45 47443.0 46 49081.5 47 48838.5 48 51163.5 49 52752.0 50 53104.5 51 52723.5 52 53858.5 53 56992.5 54 59011.0 55 57310.0 56 52156.5 57 48365.0 58 46410.0 59 42374.5 60 36987.5 61 31994.0 62 27771.0 63 22076.0 64 16949.0 65 13873.0 66 10385.0 67 8580.0 68 6378.5 69 3859.0 70 3025.5 71 2546.0 72 2171.0 73 1995.5 74 1595.0 75 1242.0 76 813.5 77 419.0 78 254.0 79 156.0 80 83.5 81 42.5 82 24.0 83 11.5 84 4.0 85 3.0 86 3.0 87 2.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04957470479589367 2 0.004624252299205444 3 4.0802226169459803E-4 4 0.02346128004743939 5 0.0031281706729919183 6 0.0031281706729919183 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.001156063074801361 20-21 0.0 22-23 0.0 24-25 3.400185514121651E-5 26-27 6.460352476831136E-4 28-29 1.0200556542364951E-4 30-31 1.0200556542364951E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.400185514121651E-5 42-43 0.003842209630957465 44-45 0.0 46-47 0.0 48-49 0.002210120584179073 50-51 1.3600742056486603E-4 52-53 0.007038384014231816 54-55 0.0 56-57 0.0 58-59 8.500463785304127E-4 60-61 0.0 62-63 0.0011900649299425778 64-65 0.0 66-67 0.001870102032766908 68-69 0.006018328359995322 70-71 0.010268560252647385 72-73 0.0 74-75 3.400185514121651E-5 76-77 8.500463785304127E-4 78-79 7.480408131067631E-4 80-81 1.0200556542364951E-4 82-83 0.0016320890467783921 84-85 0.0015640853364959591 86-87 0.0 88-89 0.0 90-91 0.0010540575093777116 92-93 0.004794261574911527 94-95 0.00710638772451425 96-97 6.800371028243301E-4 98-99 0.009418513874116973 100-101 0.01343073278078052 102-103 0.02570540248675968 104-105 0.009588523149823055 106-107 0.03189374012246108 108-109 0.027439497098961717 110-111 0.02346128004743939 112-113 0.005576304243159507 114-115 7.480408131067631E-4 116-117 9.180500888128456E-4 118-119 0.007310398855361548 120-121 0.0 122-123 1.7000927570608252E-4 124-125 0.045018456206970654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1470508.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.980198159539974 #Duplication Level Percentage of deduplicated Percentage of total 1 76.71093550329981 42.94293370478413 2 12.204151179244855 13.66381602766221 3 4.505350410163941 7.566312262174263 4 2.1828874056778766 4.887938781192467 5 1.2392920990731087 3.468790864183244 6 0.7713613092389562 2.5908575366279374 7 0.4963978849007244 1.9451916373903346 8 0.35380922041519075 1.584504821561179 9 0.25739221716784644 1.2967980589601453 >10 1.2013332492280302 11.289385341466138 >50 0.05482470762605584 2.1009202845290464 >100 0.018913121539523407 1.8961190638432612 >500 0.0016758462120406408 0.615153131329169 >1k 9.576264068803663E-4 0.9268843259853012 >5k 7.182198051602747E-4 3.2243941583112155 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9884 0.672148672431568 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8706 0.5920403017188618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5469 0.3719122915346261 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2125 0.14450788435017015 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2002 0.1361434279854309 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3600742056486603E-4 0.0 20-21 0.0 0.0 0.0 2.0401113084729904E-4 0.0 22-23 0.0 0.0 0.0 8.840482336716292E-4 0.0 24-25 0.0 0.0 0.0 0.0025161372804500216 0.0 26-27 0.0 0.0 0.0 0.004896267140335177 0.0 28-29 0.0 0.0 0.0 0.009622525004964272 0.0 30-31 0.0 6.800371028243301E-5 0.0 0.021149153897836667 0.0 32-33 0.0 6.800371028243301E-5 0.0 0.0418222818236963 0.0 34-35 0.0 6.800371028243301E-5 0.0 0.07089386796943642 0.0 36-37 0.0 6.800371028243301E-5 0.0 0.11234212938657932 0.0 38-39 0.0 6.800371028243301E-5 0.0 0.17252541298653257 0.0 40-41 0.0 6.800371028243301E-5 0.0 0.24069913254467165 0.0 42-43 0.0 6.800371028243301E-5 0.0 0.3045886183550175 0.0 44-45 0.0 6.800371028243301E-5 0.0 0.38068477016105995 0.0 46-47 0.0 6.800371028243301E-5 0.0 0.471299714112402 0.0 48-49 0.0 6.800371028243301E-5 0.0 0.5759234223819252 0.0 50-51 0.0 6.800371028243301E-5 0.0 0.6944538894042058 0.0 52-53 0.0 6.800371028243301E-5 0.0 0.803463836986946 0.0 54-55 0.0 6.800371028243301E-5 0.0 0.9180500888128457 0.0 56-57 0.0 6.800371028243301E-5 0.0 1.0600418358825658 0.0 58-59 0.0 6.800371028243301E-5 0.0 1.21556632129849 0.0 60-61 0.0 6.800371028243301E-5 0.0 1.3962521795189144 0.0 62-63 0.0 6.800371028243301E-5 0.0 1.5713277316410383 0.0 64-65 0.0 6.800371028243301E-5 0.0 1.7460292633566086 0.0 66-67 0.0 6.800371028243301E-5 0.0 1.9476602643440226 0.0 68-69 0.0 6.800371028243301E-5 0.0 2.185503241056832 0.0 70-71 0.0 6.800371028243301E-5 0.0 2.4601362250324375 0.0 72-73 0.0 6.800371028243301E-5 0.0 2.73215106616217 0.0 74-75 0.0 6.800371028243301E-5 0.0 3.0097422115350616 0.0 76-77 0.0 6.800371028243301E-5 0.0 3.3107266332451095 0.0 78-79 0.0 6.800371028243301E-5 0.0 3.652173262573206 0.0 80-81 0.0 6.800371028243301E-5 0.0 4.034796138477315 0.0 82-83 0.0 6.800371028243301E-5 0.0 4.442682392751349 0.0 84-85 0.0 6.800371028243301E-5 0.0 4.843700272286856 0.0 86-87 0.0 6.800371028243301E-5 0.0 5.253422626738515 0.0 88-89 0.0 6.800371028243301E-5 0.0 5.694664700906081 0.0 90-91 0.0 6.800371028243301E-5 0.0 6.194457969626823 0.0 92-93 0.0 6.800371028243301E-5 0.0 6.737909620348886 0.0 94-95 0.0 6.800371028243301E-5 0.0 7.273574846243611 0.0 96-97 0.0 6.800371028243301E-5 0.0 7.799141521161395 0.0 98-99 0.0 6.800371028243301E-5 0.0 8.35939008832322 0.0 100-101 0.0 6.800371028243301E-5 0.0 8.996414844393911 0.0 102-103 0.0 6.800371028243301E-5 0.0 9.676689960204229 0.0 104-105 0.0 6.800371028243301E-5 0.0 10.347478558430147 0.0 106-107 0.0 6.800371028243301E-5 0.0 10.995043889594616 0.0 108-109 0.0 6.800371028243301E-5 0.0 11.668484632521551 0.0 110-111 0.0 6.800371028243301E-5 0.0 12.406698909492501 3.400185514121651E-5 112-113 0.0 6.800371028243301E-5 0.0 13.18510337924037 6.800371028243301E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4105 0.0 73.82497 1 GTATCAA 7510 0.0 52.799877 1 ATCAACG 9830 0.0 40.133545 3 TCAACGC 9995 0.0 39.35194 4 CAACGCA 10040 0.0 39.1689 5 TATCAAC 10075 0.0 39.15892 2 AACGCAG 10295 0.0 38.487663 6 ACGCAGA 11685 0.0 33.8075 7 GTGGTAT 1545 0.0 33.526592 1 CGCAGAG 11930 0.0 33.063343 8 GTACATG 7625 0.0 31.467615 1 TGGTATC 1685 0.0 31.430578 2 ACATGGG 7560 0.0 30.617893 3 GTAAGGT 665 0.0 30.423191 4 TACATGG 7855 0.0 30.302332 2 GCAGAGT 13040 0.0 30.203276 9 GAGTACT 9015 0.0 28.311651 12-13 CATGGGG 3655 0.0 28.164772 4 TAAGGTG 720 0.0 27.268114 5 CAGAGTA 12730 0.0 27.013018 10-11 >>END_MODULE