FastQCFastQC Report
Thu 26 May 2016
SRR1364181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477484
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.042.992741
GTATCAA47150.041.767821
ATCAACG49300.037.5280843
TCAACGC50500.036.6348464
CAACGCA51100.036.2054255
GTACATG109350.034.659011
AACGCAG54000.033.9154976
TACATGG112450.031.7949312
ACATGGG110150.031.648713
TATCAAC60550.031.5379732
ACGCAGA64400.028.1603287
CGCAGAG64950.027.6472238
CATGGGA70800.026.8869844
GAGTACA81900.023.1740681
CATGGGG45250.023.137664
GCAGAGT79050.022.1915519
AGTACAT80550.021.7870982
ATGGGAG35900.021.0447335
AGAGTAC69150.019.56396110-11
TGGTATC23200.018.4623682
Produced by FastQC (version 0.11.5)