##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2477484 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.226646065120907 33.0 31.0 34.0 28.0 34.0 2 31.259894312132793 33.0 31.0 34.0 28.0 34.0 3 31.342403825816838 34.0 31.0 34.0 28.0 34.0 4 34.69385554054032 37.0 35.0 37.0 32.0 37.0 5 34.81349183284332 37.0 35.0 37.0 32.0 37.0 6 34.89320778660932 37.0 35.0 37.0 32.0 37.0 7 34.8320772202767 37.0 35.0 37.0 32.0 37.0 8 34.809667388366584 37.0 35.0 37.0 32.0 37.0 9 36.36507279159018 39.0 37.0 39.0 32.0 39.0 10-11 36.33847403252655 39.0 37.0 39.0 32.0 39.0 12-13 36.307771513357906 39.0 37.0 39.0 32.0 39.0 14-15 37.65640040460403 40.0 38.0 41.0 32.0 41.0 16-17 37.698986956121615 40.0 38.0 41.0 32.0 41.0 18-19 37.608698381099536 40.0 38.0 41.0 31.5 41.0 20-21 37.58863427574103 40.0 38.0 41.0 31.5 41.0 22-23 37.6399286534242 40.0 38.0 41.0 31.5 41.0 24-25 37.49895317184692 40.0 38.0 41.0 31.0 41.0 26-27 37.533043402096645 40.0 38.0 41.0 31.0 41.0 28-29 37.623654279906546 40.0 38.0 41.0 31.0 41.0 30-31 37.5307703298992 40.0 38.0 41.0 31.0 41.0 32-33 37.35363174898405 40.0 38.0 41.0 30.0 41.0 34-35 37.31259495520455 40.0 38.0 41.0 30.0 41.0 36-37 37.34329020893777 40.0 38.0 41.0 30.0 41.0 38-39 37.19096389724414 40.0 37.5 41.0 30.0 41.0 40-41 37.20331957744227 40.0 37.0 41.0 30.0 41.0 42-43 37.15639172644506 40.0 37.0 41.0 30.0 41.0 44-45 37.15979497748522 40.0 37.0 41.0 30.0 41.0 46-47 37.10036734848742 40.0 37.0 41.0 30.0 41.0 48-49 37.0377049861876 40.0 37.0 41.0 30.0 41.0 50-51 36.94829229976864 40.0 37.0 41.0 30.0 41.0 52-53 36.82724368754753 40.0 36.0 41.0 30.0 41.0 54-55 36.666141133504794 40.0 36.0 41.0 30.0 41.0 56-57 36.49779009672716 40.0 35.5 41.0 30.0 41.0 58-59 36.29724087017313 39.0 35.0 41.0 30.0 41.0 60-61 36.076143377717074 39.0 35.0 41.0 30.0 41.0 62-63 35.79757689656119 39.0 35.0 41.0 29.0 41.0 64-65 35.50706563594356 38.0 35.0 40.0 29.0 41.0 66-67 35.204690322924385 37.5 34.5 40.0 29.0 41.0 68-69 34.96714227014181 37.0 34.5 40.0 28.5 41.0 70-71 34.78374613115564 37.0 34.0 39.0 29.0 41.0 72-73 34.45593109783958 36.0 34.0 39.0 29.0 41.0 74-75 34.10404587880285 36.0 34.0 39.0 28.5 40.5 76-77 33.757575427328696 35.0 34.0 37.5 28.0 39.5 78-79 33.39809964463948 35.0 34.0 37.0 28.0 39.0 80-81 33.07806750719682 35.0 34.0 36.5 28.0 39.0 82-83 32.787733644293965 35.0 34.0 36.0 27.5 38.0 84-85 32.520931921255595 35.0 33.5 36.0 27.0 37.0 86-87 32.30604213791088 35.0 33.0 35.5 27.0 37.0 88-89 32.125482949637615 35.0 33.0 35.0 26.5 36.0 90-91 31.9648625379619 35.0 33.0 35.0 26.0 36.0 92-93 31.834315378020605 35.0 33.0 35.0 26.0 36.0 94-95 31.699151639324413 35.0 33.0 35.0 25.5 36.0 96-97 31.60783924336141 35.0 33.0 35.0 25.0 35.0 98-99 31.536013552458865 35.0 33.0 35.0 25.0 35.0 100-101 31.44396613661279 35.0 33.0 35.0 25.0 35.0 102-103 30.50062482744591 34.0 31.0 35.0 23.5 35.0 104-105 31.16515747427632 35.0 32.5 35.0 24.0 35.0 106-107 31.25997160829293 35.0 33.0 35.0 24.0 35.0 108-109 31.23599102960907 35.0 33.0 35.0 24.0 35.0 110-111 31.164818824258802 35.0 33.0 35.0 24.0 35.0 112-113 31.296803329506872 35.0 33.0 35.0 24.0 35.0 114-115 31.285600229910667 35.0 33.0 35.0 24.0 35.0 116-117 31.196410148360194 35.0 33.0 35.0 23.5 35.0 118-119 30.962910355828736 35.0 33.0 35.0 20.0 35.0 120-121 30.900441536655734 35.0 33.0 35.0 19.5 35.0 122-123 30.774822763739344 35.0 33.0 35.0 18.0 35.0 124-125 29.934651242954544 34.5 31.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30371684782315 1101 2 0.3617671587026656 1101 3 0.3750678645240413 1101 4 0.2964382710467177 1101 5 0.16399782722623257 1101 6 0.3362163414890773 1101 7 0.2821493808834461 1101 8 0.2636749702255301 1101 9 0.30811441241900184 1101 10-11 0.36179029942644547 1101 12-13 0.36427904782946285 1101 14-15 0.417261587721903 1101 16-17 0.4183397319075013 1101 18-19 0.5137574460862098 1101 20-21 0.5603798617807314 1101 22-23 0.4674003333698238 1101 24-25 0.7164563412300282 1101 26-27 0.7416370799791139 1101 28-29 0.570217115242265 1101 30-31 0.5995463399697414 1101 32-33 0.7778926834610331 1101 34-35 0.8309168572268604 1101 36-37 0.7063195224572141 1101 38-39 0.7424465553457367 1101 40-41 0.6885130496102505 1101 42-43 0.5840674711403437 1101 44-45 0.4583776682612637 1101 46-47 0.4789469239053261 1101 48-49 0.45314934071273427 1101 50-51 0.4151990526353657 1101 52-53 0.32355537847833205 1101 54-55 0.3725989031648709 1101 56-57 0.3304046674943706 1101 58-59 0.3227301387153929 1101 60-61 0.26653334105306925 1101 62-63 0.2940516358077545 1101 64-65 0.29525795082659556 1101 66-67 0.3100384564935439 1101 68-69 0.2782977459727647 1101 70-71 0.31789961643881526 1101 72-73 0.25933583759213974 1101 74-75 0.3005397946435622 1101 76-77 0.3082287119046754 1101 78-79 0.2879070568407798 1101 80-81 0.26509440790549377 1101 82-83 0.30097315249062717 1101 84-85 0.16205083852808855 1101 86-87 0.22014607736802105 1101 88-89 0.18406871812046433 1101 90-91 0.15546078333080615 1101 92-93 0.10675028174997081 1101 94-95 0.1357736380740704 1101 96-97 0.14770536060521877 1101 98-99 0.1422718838137449 1101 100-101 0.1206390968697697 1101 102-103 0.10109585386587483 1101 104-105 0.1371145178930675 1101 106-107 0.11688332460533957 1101 108-109 0.07377466449695547 1101 110-111 0.05267116535761929 1101 112-113 0.15614783421766276 1101 114-115 0.1861377119479144 1101 116-117 0.11157680108868107 1101 118-119 0.03350232219542093 1101 120-121 0.11535491648499985 1101 122-123 0.07631095550438616 1101 124-125 0.037475817083965524 1102 1 -0.02411540775787202 1102 2 0.03799536723419905 1102 3 -0.07969353436262949 1102 4 0.04379671555059872 1102 5 -0.0627757564689162 1102 6 0.016720731236304687 1102 7 0.06251037583171382 1102 8 0.10747962334926342 1102 9 0.1521844417395286 1102 10-11 0.13300868862208404 1102 12-13 0.10479211958601553 1102 14-15 0.19735049916290848 1102 16-17 0.0963177152650374 1102 18-19 0.2508424156283553 1102 20-21 0.40244957115005775 1102 22-23 0.2966230435731845 1102 24-25 0.3669556400869425 1102 26-27 0.403920206802276 1102 28-29 0.28445965158880426 1102 30-31 0.30373882561568877 1102 32-33 0.4749590058468698 1102 34-35 0.3391884708057873 1102 36-37 0.22442796925241026 1102 38-39 0.2936127941609925 1102 40-41 0.28098144817946746 1102 42-43 0.1893641217344566 1102 44-45 0.08713965705797477 1102 46-47 0.12434350092733126 1102 48-49 0.08459387875402058 1102 50-51 0.02137398595802864 1102 52-53 0.025955637438840995 1102 54-55 0.06577860992625517 1102 56-57 0.05077018675267908 1102 58-59 0.0691496847346258 1102 60-61 0.08081732941151643 1102 62-63 0.05520860103722214 1102 64-65 0.06192740873427027 1102 66-67 0.059447463286673496 1102 68-69 0.12740679850891468 1102 70-71 0.05961517148333684 1102 72-73 0.014412355089888251 1102 74-75 0.02289534375565694 1102 76-77 -0.07193264682145184 1102 78-79 -0.020646222117505886 1102 80-81 -0.004868839365961719 1102 82-83 -0.04787910587330657 1102 84-85 -0.004862123911806293 1102 86-87 -0.02560369579156685 1102 88-89 -0.005661847968205791 1102 90-91 -0.08555133732079057 1102 92-93 -0.0551526113921561 1102 94-95 -0.08375721680389248 1102 96-97 -0.10699742222040243 1102 98-99 -0.07184121393884979 1102 100-101 -0.1906690119769614 1102 102-103 -0.19178735644455358 1102 104-105 -0.03162412446397411 1102 106-107 -0.12468646100235503 1102 108-109 -0.12523815271508454 1102 110-111 -0.03320857131587118 1102 112-113 -0.04865093397019038 1102 114-115 0.03064329984576375 1102 116-117 0.01842442349293094 1102 118-119 -0.12268735887821691 1102 120-121 -0.1086444236156332 1102 122-123 -0.09711118095313864 1102 124-125 -0.15453890067157872 1103 1 0.17281480717026554 1103 2 -0.01248655575568236 1103 3 0.20100635552238444 1103 4 0.25788095791232024 1103 5 0.17685596208453802 1103 6 0.22119306294710128 1103 7 0.34698128412440354 1103 8 0.3853704215909417 1103 9 0.40674797616665614 1103 10-11 0.41240375192020906 1103 12-13 0.36728429278561237 1103 14-15 0.45303185662397993 1103 16-17 0.348382431209167 1103 18-19 0.5037904504717119 1103 20-21 0.5982311356464862 1103 22-23 0.559001583226177 1103 24-25 0.7559247095313566 1103 26-27 0.7132892167510079 1103 28-29 0.5657750498539968 1103 30-31 0.63058984891736 1103 32-33 0.8375447407984211 1103 34-35 0.6919113576896336 1103 36-37 0.5439434313315061 1103 38-39 0.7211916399288398 1103 40-41 0.7302068048536583 1103 42-43 0.6359725482097147 1103 44-45 0.5553951142270819 1103 46-47 0.5469613347568583 1103 48-49 0.49686094397775804 1103 50-51 0.4586692596501436 1103 52-53 0.44702394471482876 1103 54-55 0.4638348845211979 1103 56-57 0.5126250604802962 1103 58-59 0.4728507717302648 1103 60-61 0.4753657580139006 1103 62-63 0.4883976733444655 1103 64-65 0.4604702444230355 1103 66-67 0.5025631975744886 1103 68-69 0.47911102182242615 1103 70-71 0.445192107631847 1103 72-73 0.39988697306606014 1103 74-75 0.3782579115379505 1103 76-77 0.3930383930174628 1103 78-79 0.36333524625821667 1103 80-81 0.29881549447836164 1103 82-83 0.281664056881759 1103 84-85 0.22609222195161038 1103 86-87 0.26672651961636973 1103 88-89 0.28483203813116376 1103 90-91 0.3125703505332176 1103 92-93 0.33329415157114184 1103 94-95 0.3350836810370659 1103 96-97 0.3462602060611566 1103 98-99 0.2852914302362102 1103 100-101 0.3130048061661803 1103 102-103 0.25336398568678575 1103 104-105 0.3118536618169969 1103 106-107 0.3564885546512464 1103 108-109 0.2717368439062966 1103 110-111 0.3275611860073333 1103 112-113 0.33675691130266117 1103 114-115 0.30993052722720904 1103 116-117 0.2933170873719355 1103 118-119 0.0623047363532514 1103 120-121 0.17242779406177888 1103 122-123 0.2460830012651698 1103 124-125 0.1041232382460322 1104 1 0.21933092250962716 1104 2 0.16953782392860361 1104 3 0.2536266401997018 1104 4 0.29027967293623647 1104 5 0.21501368233357 1104 6 0.3315405563690774 1104 7 0.35575318644891496 1104 8 0.3406213521389745 1104 9 0.39901971719513085 1104 10-11 0.366044465234296 1104 12-13 0.35135094049594784 1104 14-15 0.4130516008890339 1104 16-17 0.3400335195106763 1104 18-19 0.5195580393420585 1104 20-21 0.5667420424876042 1104 22-23 0.5907584842355575 1104 24-25 0.7575013038306011 1104 26-27 0.7555949412398206 1104 28-29 0.5813796968100462 1104 30-31 0.5781243551398703 1104 32-33 0.7348889835331178 1104 34-35 0.6609556857278349 1104 36-37 0.5650244627608814 1104 38-39 0.6178776882449171 1104 40-41 0.6098414011448838 1104 42-43 0.5196133681139941 1104 44-45 0.4433728224565101 1104 46-47 0.3934732648637933 1104 48-49 0.367231442070576 1104 50-51 0.34473963049308765 1104 52-53 0.4005201342723126 1104 54-55 0.40854874786067086 1104 56-57 0.3401571966386072 1104 58-59 0.3562281727918375 1104 60-61 0.33794216201830807 1104 62-63 0.30969875081452614 1104 64-65 0.33890922796645384 1104 66-67 0.315060160537854 1104 68-69 0.360021173503398 1104 70-71 0.36225318412378016 1104 72-73 0.3693870525384497 1104 74-75 0.35588494431474516 1104 76-77 0.36239629998742373 1104 78-79 0.3543728139704214 1104 80-81 0.288140643775165 1104 82-83 0.2714824209292672 1104 84-85 0.28857076675726745 1104 86-87 0.32845634671767954 1104 88-89 0.3296062660028056 1104 90-91 0.34920069607039395 1104 92-93 0.3150091532311343 1104 94-95 0.3790187462937773 1104 96-97 0.381808034176327 1104 98-99 0.33106799776250995 1104 100-101 0.3896994255874766 1104 102-103 0.33803194313974316 1104 104-105 0.43372096831739526 1104 106-107 0.37102855371701793 1104 108-109 0.3143662577198221 1104 110-111 0.35094237102062564 1104 112-113 0.4409420026582893 1104 114-115 0.36533563734898067 1104 116-117 0.3315462857478799 1104 118-119 0.18364967255118714 1104 120-121 0.24456772064622 1104 122-123 0.28172056891466823 1104 124-125 0.26430815027934074 1105 1 0.07559250508536408 1105 2 0.13614462989033882 1105 3 0.1936363754086976 1105 4 0.21389244361133564 1105 5 0.07813348583174928 1105 6 0.11548329171988314 1105 7 0.08790011583464263 1105 8 0.07014575711759363 1105 9 0.2734877499693198 1105 10-11 0.2254067543870093 1105 12-13 0.1868323611562701 1105 14-15 0.14379328656202972 1105 16-17 0.20557376439702324 1105 18-19 0.30070531823575664 1105 20-21 0.3994775785207807 1105 22-23 0.4274271236535512 1105 24-25 0.6380270445916381 1105 26-27 0.5114750864237934 1105 28-29 0.39804910144098216 1105 30-31 0.41584220169706754 1105 32-33 0.6323960746581392 1105 34-35 0.618300985938788 1105 36-37 0.5547651436364234 1105 38-39 0.5876064566984667 1105 40-41 0.5660638576545196 1105 42-43 0.46115466790104165 1105 44-45 0.36541796941230587 1105 46-47 0.2999675845242109 1105 48-49 0.3231563157724864 1105 50-51 0.21502358825940604 1105 52-53 0.17186107276953067 1105 54-55 0.16432762379323407 1105 56-57 0.20032508496367285 1105 58-59 0.20321617929371882 1105 60-61 0.11934010887912194 1105 62-63 0.2752565773790181 1105 64-65 0.24995869017662642 1105 66-67 0.31843028583539024 1105 68-69 0.2866870526890324 1105 70-71 0.3112402790505726 1105 72-73 0.25727903058655244 1105 74-75 0.23718933478468074 1105 76-77 0.21638448655727416 1105 78-79 0.19868454896624854 1105 80-81 0.12469791738220692 1105 82-83 0.20152723143430507 1105 84-85 0.23302632838255732 1105 86-87 0.23846191576736686 1105 88-89 0.23449978753398426 1105 90-91 0.18302779924448132 1105 92-93 0.17081669428313617 1105 94-95 0.2496748343977231 1105 96-97 0.21883346930767544 1105 98-99 0.2639109976279883 1105 100-101 0.21574823038256952 1105 102-103 0.1713073034958228 1105 104-105 0.15111682420350903 1105 106-107 0.24823738488158753 1105 108-109 0.19810589280434954 1105 110-111 0.15264313268079022 1105 112-113 0.16903474585859968 1105 114-115 0.18899892118741235 1105 116-117 0.23764714324184766 1105 118-119 0.13122142720570196 1105 120-121 0.16983093719449727 1105 122-123 0.07319693188188481 1105 124-125 -0.030677564227609366 1106 1 0.08863504552527246 1106 2 0.12819909601404333 1106 3 0.20964455748745436 1106 4 0.1625048777073843 1106 5 0.11029157424799507 1106 6 0.21337523384890034 1106 7 0.14784006759917645 1106 8 0.23971933936832812 1106 9 0.2601025524094709 1106 10-11 0.32952471992731347 1106 12-13 0.26168202342113744 1106 14-15 0.316317816874232 1106 16-17 0.32369659112283244 1106 18-19 0.3023686601487938 1106 20-21 0.2933959575079541 1106 22-23 0.2520339437896908 1106 24-25 0.41718170788433184 1106 26-27 0.43337213583764367 1106 28-29 0.37346935223067135 1106 30-31 0.33566774622801887 1106 32-33 0.5010342623248007 1106 34-35 0.48756296301390734 1106 36-37 0.4161728607322175 1106 38-39 0.5049180966175157 1106 40-41 0.4406375267638438 1106 42-43 0.4366803794899994 1106 44-45 0.3805595923360272 1106 46-47 0.3880894558055701 1106 48-49 0.31156518229689567 1106 50-51 0.25571104501107556 1106 52-53 0.3129303453867891 1106 54-55 0.24873592863212224 1106 56-57 0.29511849181798055 1106 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72-73 -1.9358620308997345 2216 74-75 -2.01183208949643 2216 76-77 -2.0667485252152495 2216 78-79 -1.9918260427494054 2216 80-81 -1.989473033663586 2216 82-83 -2.0768088961845095 2216 84-85 -1.9976197487515606 2216 86-87 -1.9718053929680295 2216 88-89 -1.9490172143400102 2216 90-91 -2.068379358337925 2216 92-93 -2.0645999857503696 2216 94-95 -1.9815502940349425 2216 96-97 -1.9980971244907373 2216 98-99 -2.026843639594457 2216 100-101 -1.9466301910542505 2216 102-103 -2.0674543139290655 2216 104-105 -2.0270980271482344 2216 106-107 -1.9523880349398581 2216 108-109 -1.9772064222706298 2216 110-111 -1.9996877061132032 2216 112-113 -2.0545654256975325 2216 114-115 -2.0565888510456674 2216 116-117 -2.0335879251807185 2216 118-119 -1.9530400374671117 2216 120-121 -1.8513737165370223 2216 122-123 -1.843682431978504 2216 124-125 -1.7888939110197413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31839.0 3 6260.0 4 4434.0 5 3831.0 6 4687.0 7 5110.0 8 5051.0 9 5358.0 10 5993.0 11 7075.0 12 8093.0 13 7939.0 14 7761.0 15 7229.0 16 6708.0 17 6151.0 18 5812.0 19 5366.0 20 5254.0 21 5534.0 22 6517.0 23 8996.0 24 14084.0 25 21095.0 26 24985.0 27 23031.0 28 26450.0 29 32577.0 30 39033.0 31 47521.0 32 62282.0 33 84805.0 34 121849.0 35 191139.0 36 382262.0 37 827450.0 38 416523.0 39 1400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38063019399532 19.040072118180763 12.598629366833391 23.980668320990524 2 15.567975769813225 20.913195355880866 39.98885411408379 23.52997476022211 3 19.238508514656694 25.687163141961015 28.889664215101334 26.184664128280954 4 12.2594914243325 17.26262706618203 37.827712935925895 32.650168573559576 5 13.384488152983115 38.87596692623659 34.3294955104256 13.410049410354691 6 31.230574110223607 37.29847531489115 18.12248825768034 13.348462317204902 7 27.943616696219653 32.86105064398332 21.46620485164105 17.72912780815598 8 25.147649793096544 36.2820102975438 20.315005061586675 18.255334847772982 9 25.887903204322484 17.394184218875253 19.92007155698605 36.79784101981621 10-11 24.067698639415415 27.69716205475841 28.261485570909073 19.973653734917097 12-13 24.72437572694029 25.336665561350785 27.920642490589508 22.01831622111942 14-15 22.11387377888508 26.851580685469294 25.442200980868044 25.59234455477758 16-17 22.260016993519454 29.79880238070731 26.1415023320679 21.799678293705334 18-19 22.03233807299186 28.75932167266062 27.66486965854962 21.543470595797903 20-21 22.904846166903457 28.050559360967913 27.41858753256892 21.62600693955971 22-23 22.640072875701755 28.08433428760994 27.079851010932366 22.195741825755942 24-25 22.368379359847616 28.096635396582982 27.394890075036493 22.14009516853291 26-27 22.233400239959565 28.354528954205833 27.235531778494394 22.176539027340212 28-29 22.111759396505235 28.260520307560157 27.394518395804475 22.233201900130144 30-31 22.307072064844334 28.003374617908282 27.381734476909 22.30781884033839 32-33 22.032705838379247 28.156059315004295 27.388889314959897 22.42234553165656 34-35 22.56676538696311 28.174666294177868 27.17643534681829 22.082132972040732 36-37 22.554651586673295 27.858048581789763 27.104122924337766 22.48317690719918 38-39 22.236689823576686 28.173668548329626 27.32226703141172 22.267374596681965 40-41 22.512278832171297 27.87744479192841 27.440745237064462 22.169531138835826 42-43 22.210458312806477 27.916577781428494 27.3543050694758 22.518658836289234 44-45 22.316240399503364 28.11776985932457 27.247731158646943 22.318258582525118 46-47 22.377118246410348 28.072712374605562 27.081121281810905 22.469048097173182 48-49 22.212942789877705 28.162359568136385 27.11745467278732 22.507242969198586 50-51 22.16584915743807 28.122189643842603 27.200796466035154 22.51116473268417 52-53 22.508561242958468 27.981480106109363 27.121965218991832 22.38799343194034 54-55 22.243595953724558 27.84795385373021 27.39749085712902 22.51095933541621 56-57 22.18969507769783 28.328324085802237 27.106911308467126 22.375069528032814 58-59 22.315467092347134 27.902664546337657 27.14990603637808 22.631962324937124 60-61 22.36131360414774 27.925966908595246 27.39875948106431 22.313960006192705 62-63 22.21729055906087 27.959159370092728 27.341402960612633 22.48214711023377 64-65 22.096313907209918 27.896460834254995 27.294019584890734 22.713205673644357 66-67 22.111133087644657 28.278127384061452 26.95423409313834 22.656505435155548 68-69 22.250871938124686 27.94811968158111 27.224310119326343 22.576698260967852 70-71 22.039772036946516 27.933324964408524 27.313123052660853 22.713779945984104 72-73 22.25895651510525 27.777947086068643 27.294375697429622 22.668720701396484 74-75 22.12361750852134 28.03901905038494 27.124230031889564 22.713133409204158 76-77 22.15757979394343 27.92726465453738 27.309882037154765 22.605273514364423 78-79 22.277811314220187 28.01173566932651 27.127362528000575 22.583090488452733 80-81 22.155012816789437 28.070595372716195 27.18953570345213 22.584856107042242 82-83 22.37327856716726 28.05801306347434 27.17643843932572 22.392269930032672 84-85 22.243164236547518 27.928147406124754 27.145634171984977 22.683054185342748 86-87 22.180019468568233 27.71822769425023 27.39677228657587 22.704980550605665 88-89 22.38047060376369 27.71124726001838 27.250710361868297 22.657571774349634 90-91 22.231079862254056 27.917287850719656 27.297984084099053 22.55364820292723 92-93 22.385326359417167 27.729894220685857 27.30620782410102 22.578571595795953 94-95 22.25015378504967 27.539168769606587 27.343970738055223 22.86670670728852 96-97 22.342574490418475 27.530107071809134 27.307378011136052 22.819940426636347 98-99 22.09033608356306 27.570750184506014 27.18342885657948 23.15548487535145 100-101 22.027309885451885 27.66709390926982 27.415774335166777 22.889821870111522 102-103 22.345302959723508 27.569665841314382 27.26153118321933 22.82350001574278 104-105 22.17374138779478 27.63793758399259 27.275384281875205 22.91293674633743 106-107 22.016487033918683 27.84032695468337 27.245779469922056 22.897406541475895 108-109 22.107350825332354 27.988765897605063 26.835546860257686 23.068336416804897 110-111 21.981526558473366 27.99682818835588 27.111481833022964 22.910163420147786 112-113 22.120922680551793 27.997765116848942 27.157887470242265 22.723424732356996 114-115 21.97071938165846 28.160191255302692 26.97306222681527 22.896027136223577 116-117 21.972317011610297 28.11372034326098 27.06505805148915 22.848904593639578 118-119 22.020558529954926 28.179610873757778 26.82041140554447 22.979419190742828 120-121 21.900830537429748 28.236737011297773 26.940306574490247 22.922125876782232 122-123 22.157007328752705 28.20706860186486 26.890226560132934 22.745697509249503 124-125 22.15175816297545 28.193674998648554 26.78934742155601 22.865219416819983 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1726.0 1 1400.5 2 976.5 3 749.5 4 557.0 5 475.5 6 410.5 7 371.0 8 395.5 9 398.0 10 358.5 11 343.0 12 348.5 13 362.5 14 428.5 15 503.5 16 596.0 17 709.5 18 852.5 19 1111.0 20 1494.5 21 2095.5 22 2765.0 23 3608.0 24 4920.5 25 6904.0 26 10276.0 27 14354.5 28 17624.0 29 22047.5 30 28184.5 31 34599.5 32 41745.0 33 48734.0 34 56398.0 35 63046.5 36 70408.5 37 78809.0 38 82113.5 39 83050.5 40 84221.5 41 86453.0 42 88585.0 43 90775.0 44 93581.0 45 95443.0 46 94888.5 47 93206.5 48 95317.5 49 96184.0 50 93053.5 51 89249.5 52 84511.5 53 80882.5 54 76856.5 55 70219.5 56 63872.0 57 57569.5 58 51712.5 59 45728.0 60 38070.0 61 29718.5 62 22778.5 63 17227.5 64 12707.5 65 9654.5 66 7172.5 67 5683.5 68 4395.5 69 2987.0 70 2173.0 71 1681.5 72 1363.5 73 1213.5 74 965.0 75 716.5 76 492.5 77 269.0 78 165.0 79 97.0 80 56.5 81 38.5 82 26.0 83 20.0 84 16.0 85 12.0 86 10.0 87 10.0 88 10.0 89 6.5 90 4.5 91 7.5 92 8.0 93 7.0 94 5.5 95 3.0 96 2.5 97 1.0 98 1.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06248274459088333 2 0.0498085961402778 3 0.04468242781789913 4 0.04508606311887382 5 0.04359261250526744 6 0.004843623611696383 7 5.247258912671082E-4 8 0.0 9 7.265435417544573E-4 10-11 0.003753808299064696 12-13 0.002522720631091866 14-15 0.007608525423373066 16-17 0.00278508357672542 18-19 0.005065623027232466 20-21 0.00958633839814909 22-23 0.015721594972964506 24-25 0.003612535943723552 26-27 0.016145412038987943 28-29 0.007265435417544574 30-31 0.00672052776122873 32-33 0.00270435651653048 34-35 0.01824431560405637 36-37 0.015459232027330953 38-39 0.02766919988181558 40-41 0.006115074809766683 42-43 0.0015539959087525895 44-45 3.229082407797588E-4 46-47 0.0020787218000196976 48-49 0.003289627702943793 50-51 0.001614541203898794 52-53 0.001614541203898794 54-55 0.005812348334035659 56-57 0.0026639929864330104 58-59 0.007891070134055355 60-61 0.015156505551599928 62-63 0.011866877848656136 64-65 0.0025832659262380706 66-67 0.003794171829162166 68-69 0.009848701343782643 70-71 0.0026236294563355403 72-73 0.0033703547631387326 74-75 0.0011099970776804208 76-77 3.6327177087722867E-4 78-79 0.003208900642748853 80-81 0.0028052653417741545 82-83 0.0016952682640937339 84-85 0.011039425481658004 86-87 0.006357255990351501 88-89 0.0019979947398247578 90-91 9.889064873880114E-4 92-93 0.0025025388660431308 94-95 0.0 96-97 0.00139254178836271 98-99 0.02421811805848191 100-101 0.014490507304991677 102-103 0.006377437755400236 104-105 0.013360328462262521 106-107 0.032714641143999315 108-109 0.0011099970776804208 110-111 0.0014329053184601798 112-113 0.01602432144869553 114-115 0.0320688246624398 116-117 0.0498085961402778 118-119 0.35620815311017145 120-121 0.11630751197585938 122-123 0.0185470420797874 124-125 0.31901316012535297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2477484.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.25712740644931 #Duplication Level Percentage of deduplicated Percentage of total 1 84.84651393803524 53.671467421713714 2 8.97122148856828 11.349874013876791 3 2.7020270245713336 5.127674032469339 4 1.1409100147396778 2.8868276064672704 5 0.5793991458835263 1.8325562795171064 6 0.34043639146188664 1.2921016913097847 7 0.23514666921916538 1.0412291969799254 8 0.16829802710046507 0.8516839794038542 9 0.12313576232739282 0.7010293145250733 >10 0.7281837574089648 9.234818497793839 >50 0.10304421541433989 4.521221180054439 >100 0.06004304503092311 6.7112073027050645 >500 0.0014764682149085928 0.5337127562685118 >1k 1.6405202387873252E-4 0.24459672691520168 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.036353009746985E-5 0.0 3 0.0 0.0 0.0 4.036353009746985E-5 0.0 4 0.0 0.0 0.0 4.036353009746985E-5 0.0 5 0.0 0.0 0.0 4.036353009746985E-5 0.0 6 4.036353009746985E-5 0.0 0.0 4.036353009746985E-5 0.0 7 4.036353009746985E-5 0.0 0.0 4.036353009746985E-5 0.0 8 8.07270601949397E-5 0.0 0.0 4.036353009746985E-5 0.0 9 8.07270601949397E-5 0.0 0.0 4.036353009746985E-5 0.0 10-11 8.07270601949397E-5 0.0 0.0 4.036353009746985E-5 0.0 12-13 1.614541203898794E-4 0.0 0.0 4.036353009746985E-5 0.0 14-15 1.614541203898794E-4 0.0 0.0 6.054529514620478E-5 0.0 16-17 1.8163588543861433E-4 0.0 0.0 1.2109059029240955E-4 0.0 18-19 2.421811805848191E-4 0.0 0.0 1.2109059029240955E-4 0.0 20-21 2.421811805848191E-4 0.0 0.0 1.614541203898794E-4 0.0 22-23 2.421811805848191E-4 0.0 0.0 4.238170660234334E-4 0.0 24-25 2.82544710682289E-4 0.0 0.0 0.0010090882524367464 0.0 26-27 2.82544710682289E-4 0.0 0.0 0.002260357685458312 0.0 28-29 3.027264757310239E-4 0.0 0.0 0.0052472589126710805 0.0 30-31 3.229082407797588E-4 0.0 0.0 0.012835602570995412 0.0 32-33 3.430900058284937E-4 0.0 0.0 0.030413919928443535 0.0 34-35 3.6327177087722867E-4 0.0 0.0 0.0611911116277643 0.0 36-37 3.6327177087722867E-4 0.0 0.0 0.10668081004761282 0.0 38-39 4.036353009746985E-4 0.0 0.0 0.16851773815693663 0.0 40-41 4.036353009746985E-4 0.0 0.0 0.231303209223551 0.0 42-43 4.641805961209033E-4 0.0 0.0 0.29554176737367427 0.0 44-45 5.65089421364578E-4 0.0 0.0 0.37804482289290264 0.0 46-47 7.265435417544573E-4 0.0 0.0 0.4761887463249006 0.0 48-49 7.669070718519272E-4 0.0 0.0 0.5805688351569576 0.0 50-51 7.669070718519272E-4 0.0 0.0 0.6874110993249603 0.0 52-53 7.669070718519272E-4 0.0 0.0 0.7935268199512087 0.0 54-55 7.669070718519272E-4 0.0 0.0 0.9129221419795244 0.0 56-57 8.476341320468669E-4 0.0 0.0 1.0575446703187588 0.0 58-59 8.476341320468669E-4 0.0 0.0 1.2229342348931416 0.0 60-61 8.476341320468669E-4 0.0 0.0 1.4030161244230035 0.0 62-63 8.476341320468669E-4 0.0 0.0 1.5801111127256524 0.0 64-65 8.476341320468669E-4 0.0 0.0 1.7681647994497645 0.0 66-67 8.476341320468669E-4 0.0 0.0 1.9942005679955954 0.0 68-69 8.879976621443367E-4 0.0 0.0 2.2500246217533597 0.0 70-71 9.081794271930717E-4 0.0 0.0 2.5384220442997814 0.0 72-73 9.283611922418066E-4 0.0 0.0 2.8304320027899275 0.0 74-75 9.283611922418066E-4 0.0 0.0 3.121876871858708 0.0 76-77 9.283611922418066E-4 0.0 0.0 3.4444622043976874 0.0 78-79 9.283611922418066E-4 0.0 0.0 3.812658325946807 0.0 80-81 0.001130178842729156 0.0 0.0 4.21607162750597 0.0 82-83 0.001130178842729156 0.0 0.0 4.635267069333243 0.0 84-85 0.001150360607777891 0.0 0.0 5.0525452434808855 0.0 86-87 0.0011907241378753606 0.0 0.0 5.491760996236504 0.0 88-89 0.0012109059029240955 0.0 0.0 5.969766101415791 0.0 90-91 0.0012512694330215654 0.0 0.0 6.493079269129488 0.0 92-93 0.0012512694330215654 0.0 0.0 7.048279625620186 0.0 94-95 0.0012512694330215654 0.0 0.0 7.607819061596361 0.0 96-97 0.0012512694330215654 0.0 0.0 8.169316128782265 0.0 98-99 0.0013319964932165052 0.0 0.0 8.770268546638444 0.0 100-101 0.0014732688485576497 0.0 0.0 9.435176977934066 0.0 102-103 0.001614541203898794 0.0 0.0 10.13352659391544 0.0 104-105 0.0018970859145810831 0.0 0.0 10.825458408611317 0.0 106-107 0.0019374494446785528 0.0 0.0 11.52382820635774 0.0 108-109 0.0019374494446785528 0.0 0.0 12.243227403284944 0.0 110-111 0.0019374494446785528 0.0 0.0 13.01637064053693 0.0 112-113 0.0019374494446785528 0.0 0.0 13.821037794795043 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2920 0.0 42.99274 1 GTATCAA 4715 0.0 41.76782 1 ATCAACG 4930 0.0 37.528084 3 TCAACGC 5050 0.0 36.634846 4 CAACGCA 5110 0.0 36.205425 5 GTACATG 10935 0.0 34.65901 1 AACGCAG 5400 0.0 33.915497 6 TACATGG 11245 0.0 31.794931 2 ACATGGG 11015 0.0 31.64871 3 TATCAAC 6055 0.0 31.537973 2 ACGCAGA 6440 0.0 28.160328 7 CGCAGAG 6495 0.0 27.647223 8 CATGGGA 7080 0.0 26.886984 4 GAGTACA 8190 0.0 23.174068 1 CATGGGG 4525 0.0 23.13766 4 GCAGAGT 7905 0.0 22.191551 9 AGTACAT 8055 0.0 21.787098 2 ATGGGAG 3590 0.0 21.044733 5 AGAGTAC 6915 0.0 19.563961 10-11 TGGTATC 2320 0.0 18.462368 2 >>END_MODULE