FastQCFastQC Report
Thu 26 May 2016
SRR1364181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477484
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75030.30284756632131626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62670.25295824312084353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.14696361308488773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.065.1671451
GTATCAA89500.052.2135161
ATCAACG109600.041.8571553
TCAACGC110850.041.3851554
CAACGCA112350.040.826025
AACGCAG117250.039.3727576
TATCAAC117150.039.059572
TGGTATC27250.034.7195432
ACGCAGA135750.033.874897
CGCAGAG136800.033.5279128
GTGGTAT30050.032.0927471
GTACATG108900.031.3776441
ACATGGG104200.031.0068723
TACATGG110150.030.5216032
GCAGAGT154750.029.5235639
GAGTACT96700.027.25334512-13
GTACTTT102700.025.37176114-15
CAGAGTA150700.025.24442910-11
CATGGGA66050.024.9535774
AGAGTAC145150.024.32438710-11
Produced by FastQC (version 0.11.5)