FastQCFastQC Report
Thu 26 May 2016
SRR1364180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090587
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.042.3173181
GTATCAA39600.036.507991
ATCAACG37450.036.3756983
CAACGCA38400.035.3220065
TCAACGC39050.034.732374
AACGCAG41500.032.9584546
GTACATG91650.030.445091
TACATGG89150.030.2281482
TATCAAC46850.029.4586662
ACATGGG90150.028.1766783
ACGCAGA50550.026.8217287
CGCAGAG50550.026.351178
CATGGGG56350.023.224664
GAGTACA66900.020.2761551
AGTACAT69150.018.065992
GTATAGC17150.018.039151
GCAGAGT73050.017.4218679
AGAGTAC59150.017.24290810-11
CAGAGTA64600.016.84688810-11
TAGCCCT27450.016.6866444
Produced by FastQC (version 0.11.5)