##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364180_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3090587 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.11774138699218 31.0 31.0 34.0 28.0 34.0 2 31.15464926242167 33.0 31.0 34.0 27.0 34.0 3 31.272493542488853 34.0 31.0 34.0 28.0 34.0 4 34.67973268508539 37.0 35.0 37.0 32.0 37.0 5 34.75346916297778 37.0 35.0 37.0 32.0 37.0 6 34.820703963357126 37.0 35.0 37.0 32.0 37.0 7 34.7200758302549 37.0 35.0 37.0 32.0 37.0 8 34.70102637460133 37.0 35.0 37.0 32.0 37.0 9 36.23472595982575 39.0 37.0 39.0 32.0 39.0 10-11 36.17976051151447 39.0 37.0 39.0 32.0 39.0 12-13 36.144038171389454 39.0 37.0 39.0 31.5 39.0 14-15 37.468531706112785 40.0 38.0 41.0 31.5 41.0 16-17 37.494562036273365 40.0 38.0 41.0 31.5 41.0 18-19 37.390964564336805 40.0 38.0 41.0 31.0 41.0 20-21 37.356002597564796 40.0 38.0 41.0 31.0 41.0 22-23 37.38578674536585 40.0 38.0 41.0 31.0 41.0 24-25 37.23234599123079 40.0 38.0 41.0 30.0 41.0 26-27 37.24015971723171 40.0 37.5 41.0 30.0 41.0 28-29 37.325910417665 40.0 38.0 41.0 30.5 41.0 30-31 37.2223645540475 40.0 37.0 41.0 30.0 41.0 32-33 37.03683491194391 40.0 37.0 41.0 30.0 41.0 34-35 36.99136167336496 40.0 37.0 41.0 30.0 41.0 36-37 37.023313370566825 40.0 37.0 41.0 30.0 41.0 38-39 36.87095768538469 40.0 37.0 41.0 30.0 41.0 40-41 36.879636295629275 40.0 37.0 41.0 30.0 41.0 42-43 36.82148520653196 40.0 37.0 41.0 30.0 41.0 44-45 36.8242013572179 40.0 37.0 41.0 30.0 41.0 46-47 36.76926292642789 40.0 37.0 41.0 30.0 41.0 48-49 36.69474633783162 40.0 36.0 41.0 30.0 41.0 50-51 36.59102720615857 40.0 36.0 41.0 30.0 41.0 52-53 36.462432702913716 40.0 36.0 41.0 30.0 41.0 54-55 36.30399241309175 40.0 35.5 41.0 29.5 41.0 56-57 36.13076027304845 39.0 35.0 41.0 29.0 41.0 58-59 35.925282155137516 39.0 35.0 41.0 29.0 41.0 60-61 35.70025532366505 39.0 35.0 41.0 28.5 41.0 62-63 35.42424303214891 38.5 35.0 40.5 28.0 41.0 64-65 35.12993696666685 38.0 34.0 40.0 28.0 41.0 66-67 34.83365328334067 37.0 34.0 40.0 28.0 41.0 68-69 34.60524004016065 37.0 34.0 40.0 28.0 41.0 70-71 34.438350222789396 36.5 34.0 39.0 28.0 41.0 72-73 34.12664827102424 36.0 34.0 39.0 28.0 41.0 74-75 33.78669424287361 35.5 34.0 39.0 27.5 40.5 76-77 33.44343890011832 35.0 34.0 37.5 27.0 39.5 78-79 33.088615690158534 35.0 34.0 37.0 26.5 39.0 80-81 32.78029189924115 35.0 33.0 36.5 26.0 39.0 82-83 32.4920076671519 35.0 33.0 36.0 26.0 38.0 84-85 32.23405974334325 35.0 33.0 36.0 26.0 37.0 86-87 32.012985073709295 35.0 33.0 35.5 26.0 37.0 88-89 31.833905500799688 35.0 33.0 35.0 25.5 36.0 90-91 31.676191771983767 35.0 33.0 35.0 25.0 36.0 92-93 31.539314214419463 35.0 33.0 35.0 25.0 36.0 94-95 31.407358699172683 35.0 33.0 35.0 25.0 36.0 96-97 31.30855271183112 35.0 33.0 35.0 24.0 35.0 98-99 31.238115121819902 35.0 33.0 35.0 24.0 35.0 100-101 31.14486794903363 35.0 33.0 35.0 24.0 35.0 102-103 30.190043994878643 34.0 30.5 35.0 20.0 35.0 104-105 30.86364726183084 35.0 32.0 35.0 23.0 35.0 106-107 30.968438358150088 35.0 32.0 35.0 23.0 35.0 108-109 30.948751321350926 35.0 32.0 35.0 23.0 35.0 110-111 30.8782331641206 35.0 32.0 35.0 20.5 35.0 112-113 31.011476137057457 35.0 33.0 35.0 23.0 35.0 114-115 31.002686868222767 35.0 33.0 35.0 23.0 35.0 116-117 30.917442544086285 35.0 33.0 35.0 19.5 35.0 118-119 30.68553449555052 35.0 33.0 35.0 17.5 35.0 120-121 30.635011892562805 35.0 33.0 35.0 16.0 35.0 122-123 30.512611681858495 35.0 33.0 35.0 2.0 35.0 124-125 29.67753358827951 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5334381755246795 1101 2 0.5509014387671556 1101 3 0.5730840850933703 1101 4 0.5511673139696285 1101 5 0.5325182389519298 1101 6 0.5829475778830684 1101 7 0.5377684805786487 1101 8 0.5064351198555812 1101 9 0.6641906874294818 1101 10-11 0.6362904355653356 1101 12-13 0.612600795212181 1101 14-15 0.6540456945165047 1101 16-17 0.653152692102573 1101 18-19 0.7991652987053115 1101 20-21 0.8748921084766437 1101 22-23 0.8054595629885526 1101 24-25 1.0789922780724908 1101 26-27 0.9688148136614885 1101 28-29 0.8883282363437814 1101 30-31 0.9598933589139094 1101 32-33 1.0890635891810305 1101 34-35 1.0131302974584457 1101 36-37 0.9740902300384136 1101 38-39 1.060144507128392 1101 40-41 0.9570654027733667 1101 42-43 0.8569995524554841 1101 44-45 0.8029722677781166 1101 46-47 0.7392043292870909 1101 48-49 0.7669589511086627 1101 50-51 0.7449599482261675 1101 52-53 0.6466779553181468 1101 54-55 0.6676416103166645 1101 56-57 0.6634157594915067 1101 58-59 0.6336568716567115 1101 60-61 0.5331177171534378 1101 62-63 0.4748229601501066 1101 64-65 0.5296940813722983 1101 66-67 0.48929625721520864 1101 68-69 0.4484978701949629 1101 70-71 0.46702143702955823 1101 72-73 0.467365554596995 1101 74-75 0.48707979485008934 1101 76-77 0.49158288691723584 1101 78-79 0.41833514627866464 1101 80-81 0.4610327101678209 1101 82-83 0.4314971710236293 1101 84-85 0.44445770402297313 1101 86-87 0.4540105137383321 1101 88-89 0.5151965704677259 1101 90-91 0.45049175467677216 1101 92-93 0.46633274236531363 1101 94-95 0.510372415811954 1101 96-97 0.40319666549645916 1101 98-99 0.4659546254341578 1101 100-101 0.3678651332306089 1101 102-103 0.2592507581025387 1101 104-105 0.3903090022447131 1101 106-107 0.4084060014824331 1101 108-109 0.38717386884830063 1101 110-111 0.37648031188702547 1101 112-113 0.4821496529487952 1101 114-115 0.4828427727575395 1101 116-117 0.4750174771118054 1101 118-119 0.34879347645088643 1101 120-121 0.3567574466271495 1101 122-123 0.4461220285469203 1101 124-125 0.3070557974415955 1102 1 0.14246961889105592 1102 2 0.17868126024425024 1102 3 0.09253703024061721 1102 4 0.20599790623369074 1102 5 0.14376415953119448 1102 6 0.09470884677998725 1102 7 0.1676635817702845 1102 8 0.20874235793242946 1102 9 0.21825072696996983 1102 10-11 0.3247441421094024 1102 12-13 0.3530410691520842 1102 14-15 0.35482504668962633 1102 16-17 0.2925389264229423 1102 18-19 0.4007095970082801 1102 20-21 0.45657017267107136 1102 22-23 0.41209557518394035 1102 24-25 0.5699605444465021 1102 26-27 0.6032683403464247 1102 28-29 0.45828689013391966 1102 30-31 0.469201144715754 1102 32-33 0.583581476099134 1102 34-35 0.533525542455898 1102 36-37 0.4331076340052533 1102 38-39 0.5159332820453955 1102 40-41 0.4529755174147141 1102 42-43 0.45860108791868726 1102 44-45 0.3025757069370556 1102 46-47 0.31145982782275183 1102 48-49 0.246084472201062 1102 50-51 0.18795158386051725 1102 52-53 0.16525979060630647 1102 54-55 0.15503311432591005 1102 56-57 0.156523697572716 1102 58-59 0.15836196279008163 1102 60-61 0.17531228968555723 1102 62-63 0.25683528630496255 1102 64-65 0.2322622221097106 1102 66-67 0.17849417414921476 1102 68-69 0.27347857556861044 1102 70-71 0.1977545126530842 1102 72-73 0.1895369134886522 1102 74-75 0.1933898149380937 1102 76-77 0.1517354911672868 1102 78-79 0.2205565184306124 1102 80-81 0.29147274069702434 1102 82-83 0.234045874551029 1102 84-85 0.18539218315870443 1102 86-87 0.16629356758729585 1102 88-89 0.28259226236314916 1102 90-91 0.18955775115943752 1102 92-93 0.21112726707078977 1102 94-95 0.29847377773046446 1102 96-97 0.2608686753844296 1102 98-99 0.3460683634118098 1102 100-101 0.24093864957619715 1102 102-103 0.17549621732177556 1102 104-105 0.3111477181117088 1102 106-107 0.3041865024725787 1102 108-109 0.20978857639492787 1102 110-111 0.260202969940007 1102 112-113 0.32793043910628583 1102 114-115 0.3870405481263326 1102 116-117 0.3666261580539505 1102 118-119 0.22429791711754277 1102 120-121 0.3130446533274416 1102 122-123 0.32892435991219315 1102 124-125 0.2539948620682573 1103 1 0.18697192694985532 1103 2 -0.11965782088646293 1103 3 0.21473044335947833 1103 4 0.23136683278914916 1103 5 0.2517096874503437 1103 6 0.21828701629846847 1103 7 0.2750305173686627 1103 8 0.27355140274632106 1103 9 0.40771348408267727 1103 10-11 0.3376157506197117 1103 12-13 0.2841211397032737 1103 14-15 0.3032806350947439 1103 16-17 0.25644316643582243 1103 18-19 0.39360596627243893 1103 20-21 0.48944068473513624 1103 22-23 0.4273779850308159 1103 24-25 0.5348332462478282 1103 26-27 0.5382084538720022 1103 28-29 0.4156191575216681 1103 30-31 0.5716896833027789 1103 32-33 0.7019984601304614 1103 34-35 0.6474165304565105 1103 36-37 0.5032535717902107 1103 38-39 0.6741524482414079 1103 40-41 0.6428368209078741 1103 42-43 0.5972652535309777 1103 44-45 0.4844434045261323 1103 46-47 0.39089913360662365 1103 48-49 0.3987583023165797 1103 50-51 0.41008922809447057 1103 52-53 0.4025425917030603 1103 54-55 0.34228069504486314 1103 56-57 0.35070235678460193 1103 58-59 0.30730314278712 1103 60-61 0.2749636749941189 1103 62-63 0.28683788149069755 1103 64-65 0.33962659407477247 1103 66-67 0.24575701045846898 1103 68-69 0.2574830812527793 1103 70-71 0.31605731640024715 1103 72-73 0.291674037107839 1103 74-75 0.2799339346731813 1103 76-77 0.28241176974982807 1103 78-79 0.29930817887619554 1103 80-81 0.2980001455428436 1103 82-83 0.28779836324737573 1103 84-85 0.2988593828507575 1103 86-87 0.3300655635612131 1103 88-89 0.2972264673844016 1103 90-91 0.23655269961857428 1103 92-93 0.25389478418008693 1103 94-95 0.2395520182418629 1103 96-97 0.3200107101362555 1103 98-99 0.28555439042698083 1103 100-101 0.26676922733237873 1103 102-103 0.21977726410453258 1103 104-105 0.2668100741932804 1103 106-107 0.2829854520928414 1103 108-109 0.2171266809785486 1103 110-111 0.21746917446904135 1103 112-113 0.28123721056902085 1103 114-115 0.3025554203548495 1103 116-117 0.3406504392111671 1103 118-119 0.14500966849717045 1103 120-121 0.13294369350312607 1103 122-123 0.09875169723585486 1103 124-125 0.02601883874471511 1104 1 0.257143660770943 1104 2 0.3039517493573243 1104 3 0.28638967668651105 1104 4 0.3626347386457738 1104 5 0.27885350838649003 1104 6 0.2446487125478285 1104 7 0.2575811488014139 1104 8 0.2546544993906892 1104 9 0.36151226500714984 1104 10-11 0.3422101033556473 1104 12-13 0.3752792418869433 1104 14-15 0.4459492959861464 1104 16-17 0.3747629195918094 1104 18-19 0.5410069731289582 1104 20-21 0.4989812215791858 1104 22-23 0.6077479761358475 1104 24-25 0.7662733525882146 1104 26-27 0.732294120812206 1104 28-29 0.5004677259727615 1104 30-31 0.5441159603834009 1104 32-33 0.6761698795167845 1104 34-35 0.6924118618731114 1104 36-37 0.5463483534253442 1104 38-39 0.605312219453424 1104 40-41 0.6292289568689284 1104 42-43 0.4904517681341005 1104 44-45 0.4520416795765243 1104 46-47 0.3809686839551176 1104 48-49 0.35624227533975983 1104 50-51 0.3930863197196288 1104 52-53 0.37517011094776365 1104 54-55 0.38010776492373566 1104 56-57 0.26600085555914177 1104 58-59 0.35742283638246874 1104 60-61 0.2265976351620651 1104 62-63 0.27269690181605455 1104 64-65 0.3035663853933954 1104 66-67 0.35950623317651065 1104 68-69 0.3173106792333513 1104 70-71 0.28474447102584577 1104 72-73 0.24444183104368733 1104 74-75 0.2964703026639981 1104 76-77 0.29147409964296145 1104 78-79 0.27116401709041327 1104 80-81 0.27271287406568945 1104 82-83 0.2766759182010645 1104 84-85 0.28373144096816105 1104 86-87 0.2561449240005018 1104 88-89 0.27556436755498126 1104 90-91 0.23344931945461767 1104 92-93 0.22453654123451727 1104 94-95 0.3544528313893487 1104 96-97 0.29685502070623215 1104 98-99 0.3082564289504255 1104 100-101 0.40722916497763606 1104 102-103 0.268427117022636 1104 104-105 0.2956621789104261 1104 106-107 0.37383447585800766 1104 108-109 0.3147545426382088 1104 110-111 0.2780109277666938 1104 112-113 0.32749399870104057 1104 114-115 0.38427304423764497 1104 116-117 0.35563901820473376 1104 118-119 0.26516505934134926 1104 120-121 0.25194749391786075 1104 122-123 0.25135809632577377 1104 124-125 0.19324804427547804 1105 1 0.41536753205202714 1105 2 0.3852965430872537 1105 3 0.4359388718635486 1105 4 0.4086174803583873 1105 5 0.3642428313607411 1105 6 0.38091262636074674 1105 7 0.38825524379744536 1105 8 0.3946124059122127 1105 9 0.4592021278920342 1105 10-11 0.41019268582605406 1105 12-13 0.46019663280505796 1105 14-15 0.46803438272950615 1105 16-17 0.46182365546541604 1105 18-19 0.621007445424695 1105 20-21 0.7517148392209734 1105 22-23 0.596993477397767 1105 24-25 0.8867017820371217 1105 26-27 0.8487751228860461 1105 28-29 0.7570800844489085 1105 30-31 0.7379833215090557 1105 32-33 0.9267794271059628 1105 34-35 0.9136583860868441 1105 36-37 0.8105697646954297 1105 38-39 0.7779946924132801 1105 40-41 0.7562879286646265 1105 42-43 0.7372448849523252 1105 44-45 0.5794095104764239 1105 46-47 0.5954651856693545 1105 48-49 0.5459086531980688 1105 50-51 0.4787453458632811 1105 52-53 0.514343201612256 1105 54-55 0.4908792740666854 1105 56-57 0.5437065039833655 1105 58-59 0.5804849352220387 1105 60-61 0.45763159138267184 1105 62-63 0.5840932136033956 1105 64-65 0.510895071996174 1105 66-67 0.5640300213645801 1105 68-69 0.6238742758419775 1105 70-71 0.6742034848723506 1105 72-73 0.6500620872355185 1105 74-75 0.6442396654313427 1105 76-77 0.5974609038747332 1105 78-79 0.5315342785191888 1105 80-81 0.4790655959632204 1105 82-83 0.5442244928959425 1105 84-85 0.46726910281915224 1105 86-87 0.4489469527579786 1105 88-89 0.4894366533577106 1105 90-91 0.44730051196740206 1105 92-93 0.5060971702354529 1105 94-95 0.5046003486460826 1105 96-97 0.46760770008744856 1105 98-99 0.4867874893290214 1105 100-101 0.5268411871249121 1105 102-103 0.48929638653339325 1105 104-105 0.521104813114178 1105 106-107 0.5472882358751399 1105 108-109 0.5064703396988435 1105 110-111 0.4372697522294011 1105 112-113 0.47865799432682365 1105 114-115 0.5134415874797611 1105 116-117 0.5622634071296986 1105 118-119 0.5267597741290331 1105 120-121 0.5273043681386227 1105 122-123 0.4780028056049588 1105 124-125 0.4700463902899976 1106 1 0.31516624345482214 1106 2 0.4096395408361886 1106 3 0.4339500609707265 1106 4 0.38973134197064496 1106 5 0.36405841435068 1106 6 0.38119077441049143 1106 7 0.3144296794509458 1106 8 0.3305636551138349 1106 9 0.46832030246433476 1106 10-11 0.4310002391585357 1106 12-13 0.41341416246974205 1106 14-15 0.5669972083330634 1106 16-17 0.5097487166903463 1106 18-19 0.6035725918278203 1106 20-21 0.5808164381488012 1106 22-23 0.5534805550751969 1106 24-25 0.7400004294934632 1106 26-27 0.7304634986765208 1106 28-29 0.7084540625426783 1106 30-31 0.6810026047910966 1106 32-33 0.8028552167875915 1106 34-35 0.7335103966699847 1106 36-37 0.6450353007912923 1106 38-39 0.6676858196141993 1106 40-41 0.5420704191735268 1106 42-43 0.5328107073608379 1106 44-45 0.4616097195965594 1106 46-47 0.4263846196061962 1106 48-49 0.4745058459106133 1106 50-51 0.4098528413775142 1106 52-53 0.42590511091638206 1106 54-55 0.46464041140768586 1106 56-57 0.39823281395315036 1106 58-59 0.31241713542164007 1106 60-61 0.2987526022467222 1106 62-63 0.31820347099548485 1106 64-65 0.3658398410999908 1106 66-67 0.31516787812299896 1106 68-69 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2216 82-83 -2.351584756835404 2216 84-85 -2.3461159797299302 2216 86-87 -2.283706096836603 2216 88-89 -2.1856890426923066 2216 90-91 -2.317846106287835 2216 92-93 -2.2923834461962116 2216 94-95 -2.1846753522884974 2216 96-97 -2.255667927578706 2216 98-99 -2.3331662294504874 2216 100-101 -2.353067919325966 2216 102-103 -2.409567667978312 2216 104-105 -2.389312776191886 2216 106-107 -2.404283941662392 2216 108-109 -2.3093755035392967 2216 110-111 -2.4096815079874716 2216 112-113 -2.5140390570261992 2216 114-115 -2.472408855387151 2216 116-117 -2.4346761708441846 2216 118-119 -2.3595408217874763 2216 120-121 -2.2901985218936183 2216 122-123 -2.2844827442338485 2216 124-125 -2.150155627033911 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48451.0 3 9419.0 4 6056.0 5 4985.0 6 5186.0 7 6019.0 8 6602.0 9 7027.0 10 8110.0 11 9457.0 12 10585.0 13 10848.0 14 10250.0 15 9789.0 16 9103.0 17 8245.0 18 7912.0 19 7679.0 20 7581.0 21 7755.0 22 9264.0 23 12370.0 24 18166.0 25 26598.0 26 31480.0 27 31691.0 28 36992.0 29 44666.0 30 53975.0 31 65154.0 32 83278.0 33 112114.0 34 157709.0 35 242681.0 36 478632.0 37 985093.0 38 497553.0 39 2112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33238058729218 18.451824377002517 12.645728701787467 24.570066333917843 2 15.74451843729939 20.08616277500075 39.92859263259068 24.240726155109183 3 19.13196550806588 24.344341842802603 29.20397153165915 27.31972111747237 4 12.455971396986584 16.5565998799036 37.74584558710469 33.241583136005126 5 13.532033025833723 37.975112148122925 35.02922098731783 13.463633838725528 6 32.40096943497085 36.79044543530737 17.61863929641542 13.189945833306368 7 28.098442459126566 32.14678035848969 21.61906983712103 18.135707345262713 8 25.222587165480213 36.170119139179704 20.314263924620146 18.293029770719933 9 25.514000366276424 16.624387409150827 19.940612742925072 37.92099948164768 10-11 24.313182901858866 26.877454304951193 28.599609511507566 20.209753281682374 12-13 24.865160275565188 24.320090366640446 28.24353887257197 22.571210485222394 14-15 22.05992872338756 26.179504649062107 25.448229321263955 26.31233730628638 16-17 22.487461575796797 28.840284743569 26.351868629671575 22.320385050962628 18-19 22.421449065458816 28.12494438506792 27.50616658366896 21.9474399658043 20-21 23.038426851907882 27.44857883327676 27.466667615869333 22.04632669894603 22-23 22.721054224943156 27.38064899452885 27.139768264550373 22.758528515977623 24-25 22.556018624878256 27.41691446987371 27.501221489148968 22.525845416099067 26-27 22.46202517615493 27.56511790127622 27.316404790579224 22.656452131989628 28-29 22.50984306907986 27.879988577428772 27.11087417455339 22.499294178937976 30-31 22.617655824133582 27.582836605465133 27.18266070539294 22.616846865008345 32-33 22.431868982396466 27.519514540613933 27.320728965648456 22.727887511341148 34-35 22.873479727789096 27.672914555374128 27.09594614033745 22.357659576499326 36-37 22.850272531545084 27.45216094878452 27.03702888675807 22.660537632912323 38-39 22.503802523493597 27.571260803349944 27.223980649407416 22.700956023749043 40-41 22.871219593011666 27.421638178518826 27.203074804362405 22.504067424107106 42-43 22.584186135304503 27.47398316964874 27.13689079362152 22.804939901425232 44-45 22.687228054905507 27.639676339330855 27.111345514963602 22.561750090800032 46-47 22.769552994896657 27.67427141805079 26.833443131326597 22.722732455725954 48-49 22.619068805304508 27.548234057692227 27.086836673512373 22.745860463490892 50-51 22.60371874305844 27.66459253717845 27.12686984302225 22.604818876740858 52-53 22.74906573906313 27.608080064855884 26.984727124945135 22.658127071135848 54-55 22.61457412140668 27.5147609243136 27.118714816709556 22.751950137570166 56-57 22.570701091178773 27.71959070846213 27.03159768724331 22.678110513115794 58-59 22.61213903881748 27.524123403098667 27.124477407162917 22.739260150920927 60-61 22.579848663937018 27.550315236271466 27.17826119669239 22.69157490309913 62-63 22.545636703653233 27.586473588782162 27.270088449984648 22.59780125757996 64-65 22.459021485690766 27.540363731434365 27.20731319822347 22.793301584651395 66-67 22.457193103654692 27.66540122036158 26.97830675033227 22.899098925651458 68-69 22.602641650776906 27.458007751668323 27.256554981268017 22.682795616286754 70-71 22.524660850612484 27.31712983485659 27.183479446540815 22.97472986799011 72-73 22.505536726344722 27.444867730928284 27.164589413458895 22.885006129268103 74-75 22.444222514258332 27.67966274089676 27.001096726274497 22.87501801857041 76-77 22.642855582815834 27.450306617452565 27.07953448829547 22.827303311436133 78-79 22.597252283049986 27.489335843828155 27.159566451048423 22.753845422073436 80-81 22.663082148497676 27.483495458520963 27.028247413940665 22.825174979040696 82-83 22.62518057087333 27.537630492739872 27.143945947406372 22.693242988980426 84-85 22.623966708141264 27.51178304632804 27.12490433606397 22.739345909466724 86-87 22.57540835236265 27.394773568659403 27.17596743729871 22.853850641679234 88-89 22.62064908647945 27.445480058766364 27.03372400356119 22.900146851193 90-91 22.630453876483124 27.480634207558456 27.08156484630402 22.807347069654405 92-93 22.62341426669764 27.401112302565974 27.13487930533508 22.840594125401307 94-95 22.499204843610617 27.357683184456544 27.232561322493105 22.910550649439735 96-97 22.573192152261747 27.222466964984992 27.210575864171595 22.993765018581666 98-99 22.384589729776877 27.34216756846764 27.08880456580941 23.184438135946074 100-101 22.404173793504725 27.256221687150916 27.2713504588872 23.06825406045716 102-103 22.355176942276575 27.19453352405295 27.28016967380118 23.170119859869295 104-105 22.358981760426953 27.297275738133404 27.166360885858786 23.17738161558086 106-107 22.361682019147985 27.374224014603737 27.005376065665033 23.258717900583243 108-109 22.326077956459972 27.352920192256512 27.006122032635847 23.314879818647665 110-111 22.33677782084825 27.46992275141383 27.059315162194853 23.133984265543067 112-113 22.418530144655513 27.522021941037504 27.06517588427559 22.99427203003139 114-115 22.353987370656032 27.66206633156721 26.92649281629192 23.05745348148484 116-117 22.336402596315814 27.545948707207785 27.049472341104657 23.06817635537174 118-119 22.270877425419936 27.604873205568776 26.930930989440633 23.19331837957066 120-121 22.29467842393211 27.69573297561807 26.943821491357788 23.065767109092032 122-123 22.357951033421724 27.72507415795207 26.902055193063102 23.014919615563105 124-125 22.369562080722364 27.769539131172905 26.837209626930242 23.02368916117449 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 819.0 1 663.0 2 446.5 3 355.0 4 286.5 5 251.0 6 245.5 7 234.0 8 240.0 9 250.0 10 253.5 11 266.0 12 277.0 13 281.5 14 347.5 15 430.0 16 505.0 17 639.5 18 842.5 19 1159.0 20 1647.5 21 2345.5 22 3285.0 23 4597.0 24 6599.0 25 9493.5 26 13695.0 27 18795.5 28 23643.5 29 29256.5 30 35870.0 31 42586.5 32 50536.0 33 57489.0 34 63584.0 35 70502.5 36 77933.0 37 84395.5 38 89180.5 39 93614.0 40 98590.5 41 104491.0 42 108882.5 43 111148.0 44 114423.0 45 118448.5 46 119691.0 47 119608.0 48 119620.5 49 119198.5 50 116324.5 51 112340.5 52 109546.0 53 106791.0 54 104248.5 55 97983.5 56 88038.5 57 78001.0 58 70425.5 59 61993.5 60 50535.5 61 40854.5 62 33102.0 63 25653.5 64 18504.0 65 13566.5 66 10350.5 67 7903.0 68 6014.0 69 4511.0 70 3323.5 71 2359.0 72 1825.0 73 1538.5 74 1148.5 75 764.0 76 527.5 77 306.5 78 180.0 79 100.0 80 62.5 81 43.5 82 31.0 83 23.0 84 18.5 85 18.5 86 13.0 87 10.5 88 12.0 89 10.5 90 11.0 91 12.0 92 8.0 93 5.0 94 5.5 95 3.0 96 1.5 97 1.5 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06377429271526736 2 0.050152284986638464 3 0.04533119436534225 4 0.04536355067823685 5 0.04429579235271487 6 0.004109251737614893 7 3.235631289460546E-4 8 0.0 9 8.089078223651366E-4 10-11 0.0032032749765659404 12-13 0.00213551665104396 14-15 0.007522842747995769 16-17 0.002814999221830675 18-19 0.004529883805244764 20-21 0.008865629733121896 22-23 0.015158932591122658 24-25 0.0031385623507767294 26-27 0.015547208345857922 28-29 0.006940429115892871 30-31 0.006341837327342671 32-33 0.0022972982155169876 34-35 0.01800628812584794 36-37 0.01572516806677825 38-39 0.027907319871597207 40-41 0.005791780008134377 42-43 0.0012780743593369156 44-45 1.4560340802572457E-4 46-47 0.0020384477123601436 48-49 0.0027179302831468587 50-51 0.001731062739861392 52-53 0.0013751432980207321 54-55 0.0053064353147152955 56-57 0.0024590797799900147 58-59 0.007344883027075439 60-61 0.014382381081652127 62-63 0.011842410519425597 64-65 0.0021031603381493546 66-67 0.0035430162619592975 68-69 0.009205371018515252 70-71 0.001973735086570933 72-73 0.0028311773782779778 74-75 8.250859788124391E-4 76-77 3.0738497249875184E-4 78-79 0.003041493412092913 80-81 0.0023943671542008037 82-83 0.0013427869851261266 84-85 0.011065859009955067 86-87 0.005824136321028983 88-89 0.0014074996109153374 90-91 6.956607272340173E-4 92-93 0.001973735086570933 94-95 0.0 96-97 0.0013104306722315211 98-99 0.024315769140296 100-101 0.014511806333230548 102-103 0.006034452354843917 104-105 0.013282266443235541 106-107 0.03128855456908348 108-109 9.221549174962555E-4 110-111 0.0014560340802572457 112-113 0.01575752437967286 114-115 0.030964991440137424 116-117 0.04910070481756379 118-119 0.35897711340920024 120-121 0.11609445066584438 122-123 0.01815189153387366 124-125 0.32086137681935506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3090587.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.09170450499991 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45512848497742 67.51428346590657 2 8.968104417497248 14.006691202823585 3 2.373186155672271 5.559784560123472 4 0.9407959725714535 2.9387344435817577 5 0.41306573144349773 1.6128503520513637 6 0.23614010039666272 1.1064349765174317 7 0.14553812598071267 0.7955724229808084 8 0.09636611713667728 0.6020315474985288 9 0.06394862608354669 0.4494471490455352 >10 0.28822212995214136 3.946326703059283 >50 0.014057852473080295 0.7741428858241608 >100 0.005365020280218475 0.6240194081560159 >500 0.0 0.0 >1k 8.126553513077846E-5 0.06968088243149038 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.471262578921092E-5 2 0.0 0.0 0.0 0.0 6.471262578921092E-5 3 0.0 0.0 0.0 0.0 6.471262578921092E-5 4 0.0 0.0 0.0 0.0 6.471262578921092E-5 5 0.0 0.0 0.0 0.0 6.471262578921092E-5 6 0.0 0.0 0.0 0.0 9.706893868381637E-5 7 0.0 0.0 0.0 0.0 1.2942525157842185E-4 8 0.0 0.0 0.0 0.0 1.2942525157842185E-4 9 0.0 0.0 0.0 0.0 1.2942525157842185E-4 10-11 1.617815644730273E-5 0.0 0.0 0.0 1.2942525157842185E-4 12-13 3.235631289460546E-5 0.0 0.0 3.235631289460546E-5 6.794825707867147E-4 14-15 3.235631289460546E-5 0.0 0.0 3.235631289460546E-5 8.250859788124392E-4 16-17 3.235631289460546E-5 0.0 0.0 6.471262578921092E-5 0.0010030456997327693 18-19 3.235631289460546E-5 0.0 0.0 9.706893868381639E-5 0.0011001146384165856 20-21 3.235631289460546E-5 0.0 0.0 1.4560340802572457E-4 0.0013589651415734294 22-23 3.235631289460546E-5 0.0 0.0 3.0738497249875184E-4 0.0014074996109153374 24-25 3.235631289460546E-5 0.0 0.0 5.338791627609901E-4 0.0014236777673626402 26-27 3.235631289460546E-5 0.0 0.0 0.001132470951311191 0.0015854593318356675 28-29 3.235631289460546E-5 0.0 0.0 0.0027341084395941613 0.001617815644730273 30-31 3.235631289460546E-5 0.0 0.0 0.008186147162335181 0.001617815644730273 32-33 3.235631289460546E-5 0.0 0.0 0.020756574721889402 0.0016339938011775757 34-35 3.235631289460546E-5 0.0 0.0 0.041513149443778805 0.0017148845834140892 36-37 3.235631289460546E-5 0.0 0.0 0.073481186583649 0.0017148845834140892 38-39 3.235631289460546E-5 0.0 0.0 0.11701660558334065 0.0017634190527559975 40-41 3.235631289460546E-5 0.0 0.0 0.16325377670973185 0.0018119535220979058 42-43 3.235631289460546E-5 0.0 0.0 0.21065577510032885 0.0018281316785452085 44-45 3.235631289460546E-5 0.0 0.0 0.27002960926192987 0.0019737350865709326 46-47 3.235631289460546E-5 0.0 0.0 0.3420547617653216 0.002119338494596657 48-49 3.235631289460546E-5 0.0 0.0 0.41666841930028176 0.002184051120385868 50-51 4.853446934190819E-5 0.0 0.0 0.49920937349442035 0.0022972982155169876 52-53 6.471262578921092E-5 0.0 0.0 0.5788706158409389 0.002572326875121134 54-55 6.471262578921092E-5 0.0 0.0 0.6726715669224002 0.0026208613444630422 56-57 6.471262578921092E-5 0.0 0.0 0.7847376566328661 0.002637039500910345 58-59 6.471262578921092E-5 0.0 0.0 0.910102190943015 0.0026532176573576477 60-61 6.471262578921092E-5 0.0 0.0 1.0437984758235248 0.0026855739702522532 62-63 6.471262578921092E-5 0.0 0.0 1.1798244152324462 0.002944424473409097 64-65 6.471262578921092E-5 0.0 0.0 1.3224833987847617 0.0029767807863037023 66-67 6.471262578921092E-5 0.0 0.0 1.4937130066230138 0.003041493412092913 68-69 6.471262578921092E-5 0.0 0.0 1.687025798011834 0.0030738497249875184 70-71 6.471262578921092E-5 0.0 0.0 1.9012407675305694 0.0030738497249875184 72-73 6.471262578921092E-5 0.0 0.0 2.1199370863852076 0.003300343915249757 74-75 6.471262578921092E-5 0.0 0.0 2.3456709032944225 0.003640085200643114 76-77 6.471262578921092E-5 0.0 0.0 2.5978236496820832 0.0037856886086688386 78-79 6.471262578921092E-5 0.0 0.0 2.8883833394756397 0.003882757547352655 80-81 6.471262578921092E-5 0.0 0.0 3.2091476473563114 0.00402836095537838 82-83 6.471262578921092E-5 0.0 0.0 3.5420941070418017 0.004319567771429829 84-85 6.471262578921092E-5 1.617815644730273E-5 0.0 3.876949589188073 0.004335745927877131 86-87 6.471262578921092E-5 3.235631289460546E-5 0.0 4.233661760694652 0.004643130900375884 88-89 6.471262578921092E-5 3.235631289460546E-5 0.0 4.62395978498583 0.004966694029321938 90-91 6.471262578921092E-5 3.235631289460546E-5 0.0 5.046484696920035 0.0049828721857692404 92-93 6.471262578921092E-5 3.235631289460546E-5 0.0 5.493066527491379 0.0051770100631368735 94-95 6.471262578921092E-5 3.235631289460546E-5 0.0 5.944534161309809 0.0052417226889260845 96-97 6.471262578921092E-5 3.235631289460546E-5 0.0 6.412066704480411 0.005694711069450561 98-99 6.471262578921092E-5 3.235631289460546E-5 0.0 6.907474211209715 0.00598591788550201 100-101 6.471262578921092E-5 3.235631289460546E-5 0.0 7.453438456836841 0.00598591788550201 102-103 6.471262578921092E-5 3.235631289460546E-5 0.0 8.024770051773336 0.006131521293527734 104-105 6.471262578921092E-5 3.235631289460546E-5 0.0 8.604514288062429 0.006244768388658854 106-107 6.471262578921092E-5 3.235631289460546E-5 0.0 9.183384903903368 0.006244768388658854 108-109 6.471262578921092E-5 3.235631289460546E-5 0.0 9.782057583235806 0.006277124701553459 110-111 6.471262578921092E-5 3.235631289460546E-5 0.0 10.421822779944392 0.006374193640237275 112-113 6.471262578921092E-5 3.235631289460546E-5 0.0 11.10080382788124 0.006471262578921092 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2615 0.0 42.317318 1 GTATCAA 3960 0.0 36.50799 1 ATCAACG 3745 0.0 36.375698 3 CAACGCA 3840 0.0 35.322006 5 TCAACGC 3905 0.0 34.73237 4 AACGCAG 4150 0.0 32.958454 6 GTACATG 9165 0.0 30.44509 1 TACATGG 8915 0.0 30.228148 2 TATCAAC 4685 0.0 29.458666 2 ACATGGG 9015 0.0 28.176678 3 ACGCAGA 5055 0.0 26.821728 7 CGCAGAG 5055 0.0 26.35117 8 CATGGGG 5635 0.0 23.22466 4 GAGTACA 6690 0.0 20.276155 1 AGTACAT 6915 0.0 18.06599 2 GTATAGC 1715 0.0 18.03915 1 GCAGAGT 7305 0.0 17.421867 9 AGAGTAC 5915 0.0 17.242908 10-11 CAGAGTA 6460 0.0 16.846888 10-11 TAGCCCT 2745 0.0 16.686644 4 >>END_MODULE