FastQCFastQC Report
Thu 26 May 2016
SRR1364180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090587
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.17880098505558975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51020.16508190838827705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.063.5217971
GTATCAA72500.049.2701721
ATCAACG88250.039.8510483
TCAACGC90600.038.8830644
CAACGCA91000.038.7033845
AACGCAG94750.037.2971656
TATCAAC94450.037.2375072
CGCAGAG111300.031.6432518
ACGCAGA111900.031.5267477
TACATGG83050.027.9460282
GTACATG85150.027.8271851
TGGTATC23150.027.5060272
ACATGGG83050.026.4395283
GTGGTAT24650.026.325731
GCAGAGT134850.025.9847729
GAGTACT94800.024.16055712-13
CAGAGTA129000.023.7965810-11
AGAGTAC121250.023.25686310-11
CATGGGG52550.022.8741864
GTACTTT100600.022.56062514-15
Produced by FastQC (version 0.11.5)