FastQCFastQC Report
Thu 26 May 2016
SRR1364179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229026
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43450.046.2877851
GGTATCA29250.045.5681761
ATCAACG52600.037.4387323
TCAACGC54300.035.8283544
CAACGCA54800.035.5025525
GTACATG117500.034.638391
AACGCAG56550.034.2838066
TATCAAC59150.033.595212
TACATGG120900.032.9711532
ACATGGG119200.032.0932463
ACGCAGA66850.028.2884777
CGCAGAG70900.026.3370598
CATGGGG80600.025.6875764
GTAAGGT17700.023.8651394
TAAGGTG19650.023.3142015
GTATAGG7900.022.596111
GTGTAAG16100.022.5446471
GAGTACA83850.022.282611
GTGTACG3451.2842065E-920.6967241
AGGTAAG19850.020.3814012
Produced by FastQC (version 0.11.5)