FastQCFastQC Report
Thu 26 May 2016
SRR1364179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229026
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63450.19649888232550622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58210.18027107864724531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.10433486754210092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.074.132391
GTATCAA86600.056.650841
ATCAACG113750.042.6371273
TCAACGC117650.041.2231064
CAACGCA118150.041.0907445
TATCAAC118300.041.0488172
AACGCAG124500.039.2804646
GTGGTAT21700.034.2963071
ACGCAGA143200.034.0657587
CGCAGAG146050.033.3602688
GTACATG114150.030.4081291
TGGTATC22750.030.3439272
ACATGGG112500.029.3048153
TACATGG117000.028.9924032
GCAGAGT171300.028.0608779
AGAGTAC149500.025.92510210-11
GAGTACT109700.025.38048612-13
CAGAGTA159850.024.87917710-11
GTACTTT114850.024.03519814-15
AGTACTT115100.023.80208412-13
Produced by FastQC (version 0.11.5)