FastQCFastQC Report
Thu 26 May 2016
SRR1364178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298794
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.043.5359841
GTATCAA29150.042.0474431
CAACGCA29850.039.459925
TCAACGC30400.038.3537984
ATCAACG30300.038.2840463
AACGCAG31700.037.3341566
GTACATG78000.037.225121
TACATGG82850.033.8221742
ACATGGG82700.033.0166553
TATCAAC39200.030.8093912
ACGCAGA40200.028.9950337
CGCAGAG41950.027.5019448
CATGGGG50150.026.6893184
GTATAGG8350.024.9397741
GAGTACA59900.023.9387321
AGTACAT59000.022.3858762
GCAGAGT52550.021.8422369
CATGGGA45300.020.7484074
AGAGTAC49550.019.02383610-11
ATGGGGA32450.018.3324555
Produced by FastQC (version 0.11.5)