FastQCFastQC Report
Thu 26 May 2016
SRR1364178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298794
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49750.2164178260426989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42710.18579307236751097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26960.11727888623339021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.066.988021
GTATCAA62600.051.2614821
ATCAACG74550.042.2275353
TCAACGC76200.041.2341654
CAACGCA76550.041.117115
AACGCAG79950.039.7380526
TATCAAC82700.038.356392
GTACATG80450.033.8934821
ACGCAGA94800.033.448327
CGCAGAG96500.032.7974248
TACATGG85100.031.190432
ACATGGG82850.030.957973
GCAGAGT110500.028.2652249
TGGTATC18250.026.4142462
GAGTACT71650.026.19792712-13
GTGGTAT20500.025.5567461
CAGAGTA109050.024.8222510-11
GTACTTT76650.024.21732714-15
AGAGTAC105500.024.19135910-11
CATGGGG50800.024.0142154
Produced by FastQC (version 0.11.5)