FastQCFastQC Report
Thu 26 May 2016
SRR1364177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858202
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30360.16338374407088144No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.055.023031
GTATCAA29600.042.814291
ATCAACG32200.037.1339263
TCAACGC32550.036.5489274
CAACGCA32500.036.4231075
AACGCAG34800.034.3466076
TATCAAC36300.033.106292
GTACATG70600.032.108581
TACATGG72650.030.135472
ACATGGG71450.029.2237263
ACGCAGA42850.027.8933457
CGCAGAG43600.027.1407558
CATGGGG39750.024.3919264
GAGTACA55500.023.0486971
TGTCGCG650.002060057422.87114586-87
CATGGGA38850.021.8947834
AGTACAT56600.021.3375912
GCAGAGT54900.021.230049
TAGCCCT15550.019.8915484
CAGAGTA51600.019.59143610-11
Produced by FastQC (version 0.11.5)