FastQCFastQC Report
Thu 26 May 2016
SRR1364177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858202
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72940.39252998328491734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71470.3846191103012482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38830.20896544078630847No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.10332568794996454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.071.473981
GTATCAA68550.048.8113481
ATCAACG86800.038.3242453
TCAACGC87400.038.1973234
CAACGCA88450.037.6674775
AACGCAG91000.036.9388476
TATCAAC90100.036.791462
TGGTATC17400.035.5713772
GTGGTAT19450.033.67171
ACGCAGA105750.031.7286557
CGCAGAG106400.031.4789098
GTACATG68600.030.9838981
ACATGGG69400.029.2399373
TACATGG71950.029.115782
GAGTACT83550.027.52047712-13
GCAGAGT121500.027.4687889
CAGAGTA117250.026.53638610-11
GTACTTT91450.025.33825514-15
AGAGTAC111000.024.49323310-11
CATGGGG38300.024.0832124
Produced by FastQC (version 0.11.5)