FastQCFastQC Report
Thu 26 May 2016
SRR1364176_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364176_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025006
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34540.1705673958496913No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.057.004791
GTATCAA40050.046.64551
ATCAACG44150.039.6156773
CAACGCA44850.038.9992945
AACGCAG46500.037.8563736
TACATGG95950.037.201182
TCAACGC46900.037.1650284
GTACATG98300.036.980291
ACATGGG96250.035.2927743
TATCAAC51800.034.3393332
CATGGGA53100.031.5932484
CGCAGAG56850.030.5451268
ACGCAGA57650.030.2244117
CATGGGG44650.028.112584
ATGGGAT24250.025.0242085
GCAGAGT67600.024.1949
ATGGGAG26600.023.2607445
TAGCCCG1802.17986E-523.1364865
AGAGTAC60300.021.89562610-11
CAGAGTA64700.021.32580410-11
Produced by FastQC (version 0.11.5)