##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364176_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2025006 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94506436030313 31.0 31.0 34.0 27.0 34.0 2 30.912967171455293 33.0 31.0 34.0 27.0 34.0 3 31.049027509054294 33.0 31.0 34.0 27.0 34.0 4 34.37846653293867 37.0 35.0 37.0 30.0 37.0 5 34.54184530811267 37.0 35.0 37.0 31.0 37.0 6 34.573683238469414 37.0 35.0 37.0 31.0 37.0 7 34.49176595032311 37.0 35.0 37.0 31.0 37.0 8 34.46213542083332 37.0 35.0 37.0 31.0 37.0 9 35.89872573217067 39.0 37.0 39.0 30.0 39.0 10-11 35.88298874176175 39.0 37.0 39.0 30.0 39.0 12-13 35.84168120983345 39.0 37.0 39.0 30.0 39.0 14-15 37.1338247886673 40.0 38.0 41.0 31.0 41.0 16-17 37.17628046534183 40.0 38.0 41.0 31.0 41.0 18-19 37.0922960228266 40.0 38.0 41.0 30.5 41.0 20-21 37.080177293301844 40.0 38.0 41.0 30.0 41.0 22-23 37.13380849241928 40.0 37.5 41.0 30.0 41.0 24-25 37.00195159915576 40.0 37.0 41.0 30.0 41.0 26-27 37.03318360538191 40.0 37.0 41.0 30.0 41.0 28-29 37.13137121569022 40.0 37.0 41.0 30.0 41.0 30-31 37.02826806439092 40.0 37.0 41.0 30.0 41.0 32-33 36.84679230580058 40.0 37.0 41.0 30.0 41.0 34-35 36.804969466757136 40.0 37.0 41.0 30.0 41.0 36-37 36.827804460826286 40.0 37.0 41.0 30.0 41.0 38-39 36.663413589885664 40.0 36.5 41.0 29.5 41.0 40-41 36.661932112793735 40.0 36.5 41.0 30.0 41.0 42-43 36.58871134208985 40.0 36.0 41.0 29.0 41.0 44-45 36.575801997623714 40.0 36.0 41.0 29.5 41.0 46-47 36.50396813639071 40.0 36.0 41.0 29.0 41.0 48-49 36.415861977692906 40.0 36.0 41.0 29.0 41.0 50-51 36.31531067068443 40.0 35.5 41.0 29.0 41.0 52-53 36.18223007734298 40.0 35.0 41.0 29.0 41.0 54-55 36.02030314971906 39.0 35.0 41.0 29.0 41.0 56-57 35.84609181404895 39.0 35.0 41.0 29.0 41.0 58-59 35.63111492015332 39.0 35.0 41.0 28.0 41.0 60-61 35.408900516837974 38.5 35.0 40.5 28.0 41.0 62-63 35.1260403672878 38.0 34.0 40.0 28.0 41.0 64-65 34.82043633450962 37.5 34.0 40.0 28.0 41.0 66-67 34.51616414963709 37.0 34.0 40.0 27.0 41.0 68-69 34.28143052415647 37.0 34.0 39.5 26.5 41.0 70-71 34.1186695249298 36.0 34.0 39.0 27.0 41.0 72-73 33.80363860650289 36.0 34.0 39.0 26.5 41.0 74-75 33.4534226071429 35.5 34.0 38.5 26.0 40.0 76-77 33.10961053942556 35.0 33.5 37.0 26.0 39.5 78-79 32.75495159026689 35.0 33.0 37.0 26.0 39.0 80-81 32.445637691937705 35.0 33.0 36.5 26.0 39.0 82-83 32.1560348463165 35.0 33.0 36.0 25.5 37.5 84-85 31.89067489182748 35.0 33.0 36.0 25.0 37.0 86-87 31.67696293245551 35.0 33.0 35.0 25.0 37.0 88-89 31.492493108662394 35.0 33.0 35.0 24.5 36.0 90-91 31.322503488878553 35.0 33.0 35.0 24.0 36.0 92-93 31.179565887706012 35.0 32.0 35.0 24.0 36.0 94-95 31.03700112493494 35.0 32.0 35.0 23.0 36.0 96-97 30.933018223155884 35.0 32.0 35.0 22.5 35.0 98-99 30.85150562516852 35.0 32.0 35.0 20.0 35.0 100-101 30.747356797955167 35.0 32.0 35.0 20.0 35.0 102-103 29.75421159245948 33.5 30.0 34.5 18.5 35.0 104-105 30.43528315471658 34.5 31.5 35.0 19.0 35.0 106-107 30.534124590248126 35.0 32.0 35.0 18.5 35.0 108-109 30.50382838371837 35.0 32.0 35.0 18.0 35.0 110-111 30.41456050006765 35.0 32.0 35.0 17.5 35.0 112-113 30.546274924617506 35.0 32.0 35.0 17.5 35.0 114-115 30.527322882006274 35.0 32.0 35.0 16.0 35.0 116-117 30.42905601267354 35.0 32.0 35.0 7.5 35.0 118-119 30.170164187167842 35.0 32.0 35.0 2.0 35.0 120-121 30.092601207107535 35.0 32.0 35.0 2.0 35.0 122-123 29.943551278366584 35.0 32.0 35.0 2.0 35.0 124-125 29.05733341037014 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4080561251093968 1101 2 0.439447407592489 1101 3 0.43376134229831464 1101 4 0.3871029765164735 1101 5 0.289538085229033 1101 6 0.2870955022978947 1101 7 0.3809180515323334 1101 8 0.25008733032696995 1101 9 0.3945390074677988 1101 10-11 0.3074738277485878 1101 12-13 0.32191812136147746 1101 14-15 0.3352765418893213 1101 16-17 0.36725616371410297 1101 18-19 0.5065906613739131 1101 20-21 0.619619983746432 1101 22-23 0.5971297421025099 1101 24-25 0.8460332251246498 1101 26-27 0.7487113971584733 1101 28-29 0.6335568109516032 1101 30-31 0.7012074726712285 1101 32-33 0.8792427893179777 1101 34-35 0.9106465680540836 1101 36-37 0.8172375782584496 1101 38-39 0.8850315779295528 1101 40-41 0.7889734417011454 1101 42-43 0.636330225084869 1101 44-45 0.5854629186654208 1101 46-47 0.5384535924914431 1101 48-49 0.5458960981075762 1101 50-51 0.49743874220020956 1101 52-53 0.39047193414663184 1101 54-55 0.4128025673688924 1101 56-57 0.4721756936817485 1101 58-59 0.47573469781379174 1101 60-61 0.30703496666203733 1101 62-63 0.3008112625144008 1101 64-65 0.3320839478008253 1101 66-67 0.305478773078967 1101 68-69 0.27188156552248444 1101 70-71 0.3128518305803425 1101 72-73 0.2511766672429516 1101 74-75 0.31225490492191454 1101 76-77 0.395968471568807 1101 78-79 0.32906204850741005 1101 80-81 0.24852856061791329 1101 82-83 0.26647874960598017 1101 84-85 0.1766842400232278 1101 86-87 0.2370973248367747 1101 88-89 0.20090041003009418 1101 90-91 0.16157684029007058 1101 92-93 0.07848149861772313 1101 94-95 0.17826411975826417 1101 96-97 0.14264296082827954 1101 98-99 0.1649489532856414 1101 100-101 0.13118783311944782 1101 102-103 0.03181034553231754 1101 104-105 0.14597199952110174 1101 106-107 0.13109867511328588 1101 108-109 0.1198924194208395 1101 110-111 0.04606957270108225 1101 112-113 0.10130897100484049 1101 114-115 0.19361832413908786 1101 116-117 0.1630451604026142 1101 118-119 0.011636913580215236 1101 120-121 0.019902688737072793 1101 122-123 0.04835469189250219 1101 124-125 -0.08694588175832862 1102 1 0.19328493149520654 1102 2 0.17935075271650902 1102 3 0.23888394134586477 1102 4 0.21613322537270108 1102 5 0.18504387048457716 1102 6 0.17844171282030885 1102 7 0.2727105297582497 1102 8 0.2711621767857224 1102 9 0.27206878447370997 1102 10-11 0.24877236710904072 1102 12-13 0.2330964467516381 1102 14-15 0.2744903110466552 1102 16-17 0.30374018916545253 1102 18-19 0.46227708053133654 1102 20-21 0.5279574238688411 1102 22-23 0.500643067240496 1102 24-25 0.6179379873187756 1102 26-27 0.5937288948144186 1102 28-29 0.4543614904427926 1102 30-31 0.5000694414142544 1102 32-33 0.6494344887629993 1102 34-35 0.566492559513982 1102 36-37 0.45121687761086804 1102 38-39 0.5216591333803251 1102 40-41 0.4171891790789246 1102 42-43 0.46963161560010036 1102 44-45 0.24425565028680296 1102 46-47 0.3228363496893678 1102 48-49 0.22337288690032153 1102 50-51 0.1018018232703568 1102 52-53 0.13221680197689523 1102 54-55 0.21176121529442327 1102 56-57 0.20302425251780676 1102 58-59 0.06108619135828519 1102 60-61 0.12340024261091287 1102 62-63 0.05189214306668788 1102 64-65 0.00591870843325637 1102 66-67 0.027466025524653048 1102 68-69 0.22072636778590748 1102 70-71 0.17135908212669904 1102 72-73 0.141269086707851 1102 74-75 0.20759686423402002 1102 76-77 0.266730401968303 1102 78-79 0.3961724712038688 1102 80-81 0.2963933240030556 1102 82-83 0.2661544398655664 1102 84-85 0.18741125410702608 1102 86-87 0.20825624147088106 1102 88-89 0.25066077812802945 1102 90-91 0.16131961654430071 1102 92-93 0.2589239258910965 1102 94-95 0.22905484123046094 1102 96-97 0.29601843761585656 1102 98-99 0.27632677443705234 1102 100-101 0.26412948714165907 1102 102-103 0.14416231123095358 1102 104-105 0.22884507877433435 1102 106-107 0.21514138409921912 1102 108-109 0.1976890212478395 1102 110-111 0.21711923152869517 1102 112-113 0.17862393914202812 1102 114-115 0.185425264744282 1102 116-117 0.18256572645284663 1102 118-119 0.04431909550358171 1102 120-121 0.10234493492687591 1102 122-123 0.1117956808275693 1102 124-125 0.08816679415251372 1103 1 0.26464417504488935 1103 2 -0.11629653265011441 1103 3 0.21664553224236371 1103 4 0.23307541143591948 1103 5 0.27896252144498845 1103 6 0.08040524377678082 1103 7 0.2568826236994397 1103 8 0.2700883639845131 1103 9 0.33302235364990196 1103 10-11 0.2845313668593903 1103 12-13 0.32889806780553954 1103 14-15 0.31304547002361716 1103 16-17 0.18212348081756602 1103 18-19 0.28315467097709046 1103 20-21 0.4316174022406756 1103 22-23 0.45839761061791506 1103 24-25 0.6507249511516804 1103 26-27 0.6672010244289979 1103 28-29 0.5292467347931051 1103 30-31 0.6575554070768987 1103 32-33 0.7282784839592296 1103 34-35 0.7412649314959836 1103 36-37 0.5368427402626921 1103 38-39 0.7827625557228259 1103 40-41 0.7308342219399151 1103 42-43 0.6504480445375123 1103 44-45 0.5245040442057629 1103 46-47 0.5783713603571385 1103 48-49 0.5018255331129993 1103 50-51 0.39030247603746915 1103 52-53 0.4403538849390145 1103 54-55 0.32499311913935713 1103 56-57 0.41996849306980266 1103 58-59 0.38744073759953324 1103 60-61 0.36314152644226994 1103 62-63 0.33532816971029433 1103 64-65 0.37235390269762547 1103 66-67 0.4336206513306493 1103 68-69 0.4403035913497675 1103 70-71 0.5216017297028088 1103 72-73 0.5336009875021972 1103 74-75 0.5197010405116487 1103 76-77 0.496877970294058 1103 78-79 0.46846731649152673 1103 80-81 0.5418642034923522 1103 82-83 0.34586592738392596 1103 84-85 0.4076456650508504 1103 86-87 0.38822435331476157 1103 88-89 0.45366319557648893 1103 90-91 0.4524996962211141 1103 92-93 0.3759694589655673 1103 94-95 0.4015421236336394 1103 96-97 0.43670845946192216 1103 98-99 0.4643835953709221 1103 100-101 0.453198299441695 1103 102-103 0.40839235420712683 1103 104-105 0.502549676906991 1103 106-107 0.5108179303666205 1103 108-109 0.4890451643871856 1103 110-111 0.5454410441605617 1103 112-113 0.5807195799526426 1103 114-115 0.5334833059199724 1103 116-117 0.5925557715208605 1103 118-119 0.5661085676805122 1103 120-121 0.53401793004155 1103 122-123 0.4681501212315169 1103 124-125 0.4076600327054294 1104 1 0.39690751031110594 1104 2 0.44135837303172565 1104 3 0.3922235702655712 1104 4 0.43310764154688997 1104 5 0.4288132317559388 1104 6 0.4506740761959307 1104 7 0.39830009658129484 1104 8 0.4294839499852685 1104 9 0.4725133028613513 1104 10-11 0.47898286308564764 1104 12-13 0.5273383870732289 1104 14-15 0.5307267493694354 1104 16-17 0.5355350288154668 1104 18-19 0.7120960043141267 1104 20-21 0.7837925185604036 1104 22-23 0.8441048742768729 1104 24-25 1.0058870750836277 1104 26-27 1.0103955810083391 1104 28-29 0.8060880706271618 1104 30-31 0.8085354429045779 1104 32-33 0.9439239370165566 1104 34-35 0.9057054315667372 1104 36-37 0.7338430510034968 1104 38-39 0.8683114201778679 1104 40-41 0.7871851838029542 1104 42-43 0.6772211168115732 1104 44-45 0.5604926392781167 1104 46-47 0.5890448679457876 1104 48-49 0.6062260646092597 1104 50-51 0.5956832514467791 1104 52-53 0.6610493963989725 1104 54-55 0.5392806995874722 1104 56-57 0.5275268933834951 1104 58-59 0.5610291327249683 1104 60-61 0.49789743537301945 1104 62-63 0.5764592824491004 1104 64-65 0.6300672225655148 1104 66-67 0.6431334240727651 1104 68-69 0.5927665244101021 1104 70-71 0.5100322990151156 1104 72-73 0.563615567350098 1104 74-75 0.5971565403003325 1104 76-77 0.4452003167201184 1104 78-79 0.5825164773526126 1104 80-81 0.5283903293035337 1104 82-83 0.5379045582007151 1104 84-85 0.5341065399157188 1104 86-87 0.5178524608384762 1104 88-89 0.5457933955141527 1104 90-91 0.5425850528378113 1104 92-93 0.5508669422424362 1104 94-95 0.5042167026307034 1104 96-97 0.49889245871466414 1104 98-99 0.39398548413865697 1104 100-101 0.5011989112126685 1104 102-103 0.5390387505847762 1104 104-105 0.5068254843475692 1104 106-107 0.5200265169860856 1104 108-109 0.4923692702906095 1104 110-111 0.4388841124671252 1104 112-113 0.5139707080065286 1104 114-115 0.47802422105166187 1104 116-117 0.4684309438849077 1104 118-119 0.36677764305087734 1104 120-121 0.3846076670863887 1104 122-123 0.3365768779575298 1104 124-125 0.30505579812201233 1105 1 0.20862224913868133 1105 2 0.22677351163270743 1105 3 0.2689574852888086 1105 4 0.19088001579512337 1105 5 0.08031606284831128 1105 6 0.04101081324414224 1105 7 0.18070402931737561 1105 8 0.08748096672224648 1105 9 0.10043563029130098 1105 10-11 0.054269518482414014 1105 12-13 0.1372701818520241 1105 14-15 0.1557490250882907 1105 16-17 0.1842976098272615 1105 18-19 0.3290103672487632 1105 20-21 0.474283580891111 1105 22-23 0.42411095212539607 1105 24-25 0.7429098697089032 1105 26-27 0.7078509757048934 1105 28-29 0.5469216403711528 1105 30-31 0.5237449221936572 1105 32-33 0.7469685396267138 1105 34-35 0.6743754016981924 1105 36-37 0.5094091797012723 1105 38-39 0.5961839442625774 1105 40-41 0.5249669239529311 1105 42-43 0.4784852329963343 1105 44-45 0.38477099576324036 1105 46-47 0.3490637793016589 1105 48-49 0.3976422369831454 1105 50-51 0.18357375545932797 1105 52-53 0.3086218817003612 1105 54-55 0.3727565152266763 1105 56-57 0.3410482659538161 1105 58-59 0.3336914503334043 1105 60-61 0.24058791353002107 1105 62-63 0.30095403323485925 1105 64-65 0.3704583078789483 1105 66-67 0.35519237606135334 1105 68-69 0.2723038246243945 1105 70-71 0.29185335979270377 1105 72-73 0.2788386252889694 1105 74-75 0.23547708351813412 1105 76-77 0.2673371742479915 1105 78-79 0.278274260275559 1105 80-81 0.18370483327657894 1105 82-83 0.25433241270223306 1105 84-85 0.21884665129206127 1105 86-87 0.27196999000003075 1105 88-89 0.3169791752338931 1105 90-91 0.23316072923412534 1105 92-93 0.23278213336249465 1105 94-95 0.26136238291432434 1105 96-97 0.27173690978259657 1105 98-99 0.326730936749847 1105 100-101 0.34628464074751264 1105 102-103 0.26981542241955836 1105 104-105 0.24084363799234865 1105 106-107 0.30375981075803793 1105 108-109 0.23593731077249203 1105 110-111 0.2441966862519358 1105 112-113 0.264906232088542 1105 114-115 0.3236229093316503 1105 116-117 0.2847128784003523 1105 118-119 0.29730977574728 1105 120-121 0.34493388236431954 1105 122-123 0.38711509748008055 1105 124-125 0.3218191462714124 1106 1 0.36186428798895065 1106 2 0.35511065882760917 1106 3 0.30165980455272035 1106 4 0.3798276043773967 1106 5 0.3517471706564592 1106 6 0.25959592648641916 1106 7 0.274581421042825 1106 8 0.256896343794331 1106 9 0.3939814551710228 1106 10-11 0.46694870722100745 1106 12-13 0.4738767117180487 1106 14-15 0.5228654763995806 1106 16-17 0.4796751326513373 1106 18-19 0.5825547299802594 1106 20-21 0.6129819721432881 1106 22-23 0.5249491588280861 1106 24-25 0.6772997608778013 1106 26-27 0.6163755746997452 1106 28-29 0.5290178002532144 1106 30-31 0.585845215667689 1106 32-33 0.7300971630471409 1106 34-35 0.7470568111431248 1106 36-37 0.5985033678819391 1106 38-39 0.6715261336701346 1106 40-41 0.5269094923097413 1106 42-43 0.4997004540692984 1106 44-45 0.44012017709764706 1106 46-47 0.3875637361518045 1106 48-49 0.246552139519423 1106 50-51 0.3507250564689244 1106 52-53 0.3395055304307988 1106 54-55 0.3351450607794604 1106 56-57 0.2953185907760911 1106 58-59 0.2732710882153597 1106 60-61 0.2974324437489386 1106 62-63 0.28801182271959647 1106 64-65 0.29970402078888725 1106 66-67 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2216 74-75 -2.0299295679136797 2216 76-77 -1.9006956203549983 2216 78-79 -1.9435185643464976 2216 80-81 -1.9717167910178546 2216 82-83 -1.9352220755042708 2216 84-85 -1.9752270506344871 2216 86-87 -1.974792984539956 2216 88-89 -1.8540387762441988 2216 90-91 -1.9076524180235417 2216 92-93 -1.9143149182809474 2216 94-95 -1.7993947124950296 2216 96-97 -1.9001106593993704 2216 98-99 -1.9546731484718798 2216 100-101 -1.9476889877072487 2216 102-103 -2.0137687817703664 2216 104-105 -2.013160861894516 2216 106-107 -2.034844930942487 2216 108-109 -2.028055308548794 2216 110-111 -1.9887624031538031 2216 112-113 -1.9701063605973594 2216 114-115 -2.0695157295442534 2216 116-117 -1.8461755316009665 2216 118-119 -1.768908593745941 2216 120-121 -1.7463912630554113 2216 122-123 -1.7431435543358944 2216 124-125 -1.6625209461426778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32574.0 3 6501.0 4 4544.0 5 4101.0 6 4744.0 7 5111.0 8 5026.0 9 5143.0 10 5955.0 11 7150.0 12 8100.0 13 8239.0 14 7999.0 15 7785.0 16 6696.0 17 6150.0 18 5552.0 19 5357.0 20 5247.0 21 5573.0 22 6245.0 23 8834.0 24 13735.0 25 20457.0 26 23994.0 27 22237.0 28 24643.0 29 30637.0 30 36231.0 31 43529.0 32 56448.0 33 75393.0 34 107575.0 35 167201.0 36 324454.0 37 632191.0 38 282587.0 39 1068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.69598120685847 19.46087323474577 12.87961706108117 23.963528497314595 2 15.327594759590585 21.063171084070664 40.2359923397847 23.37324181655405 3 18.22296418676163 26.488446959965533 29.521268186255718 25.767320667017117 4 11.936733194569536 17.399211388183076 38.15933594952331 32.50471946772408 5 12.563325003631276 39.89823534817265 34.64637302243684 12.892066625759233 6 30.691559393985486 38.14550037483295 18.034601180699113 13.128339050482444 7 27.44193557307881 33.961418119182696 21.695394352175217 16.901251955563282 8 25.29780158676073 36.796928009102196 20.30384107503879 17.601429329098284 9 26.32110985623089 17.302910691102095 19.897599140338464 36.47838031232855 10-11 23.86917779687856 28.156335164917923 28.23396742062633 19.74051961757719 12-13 24.386929155517212 25.86938587851203 27.89417711404655 21.84950785192421 14-15 21.956165403767905 27.280070168541798 25.337446582172706 25.426317845517595 16-17 21.83872966206678 30.09254835263142 26.349600716860216 21.719121268441583 18-19 21.808629372936316 29.122380485338027 27.67640641652073 21.392583725204926 20-21 22.751202156659044 28.276517391730582 27.581855676009244 21.390424775601126 22-23 22.522053085456324 28.16838382740806 27.30100362530005 22.008559461835567 24-25 22.139714528262473 28.288991860949196 27.713070433580633 21.858223177207694 26-27 21.835661702059355 28.613153287087002 27.421647531813903 22.129537479039744 28-29 21.968004918901098 28.577185632956635 27.461098826205 21.99371062193726 30-31 22.22002179900566 28.29779101104618 27.331676266493094 22.15051092345507 32-33 21.753003040078067 28.474879354926646 27.505669281384016 22.266448323611275 34-35 22.28699974612545 28.529770001511395 27.32310031205825 21.86012994030491 36-37 22.49325939868749 28.079549714496828 27.231387222911774 22.195803663903913 38-39 21.974344687505866 28.538213266391825 27.293285004252997 22.19415704184931 40-41 22.288333429635763 28.400303031649916 27.443106252200757 21.86825728651356 42-43 21.99766465618371 28.26457386148285 27.23021260320217 22.507548879131267 44-45 22.020323930544265 28.400046024645903 27.442096353485084 22.137533691324748 46-47 22.348815009533553 28.532958542741486 26.9390696969263 22.179156750798658 48-49 21.98505680060269 28.536404510861956 27.221102707272752 22.257435981262606 50-51 22.028671169469437 28.443411996576724 27.283784724742848 22.244132109210994 52-53 22.166720659289428 28.318348734399752 27.268677826019967 22.24625278029085 54-55 22.038499128589045 28.36372283353508 27.27422455783734 22.32355348003854 56-57 22.016432605944626 28.661403129726224 27.045649557085095 22.27651470724405 58-59 22.001899906486276 28.35001739646633 27.154118369557246 22.493964327490147 60-61 21.932689455837163 28.550342143781638 27.24990189713288 22.267066503248323 62-63 21.887733822019058 28.33097213706084 27.664890069171488 22.116403971748618 64-65 21.79875636036265 28.26797389655563 27.407653596260833 22.525616146820887 66-67 21.863399250243095 28.57967932607808 27.073375135745902 22.483546287932924 68-69 22.037864835752607 28.276490148811156 27.4048897139987 22.280755301437537 70-71 21.92975070193247 28.131250939840108 27.427824600711475 22.51117375751594 72-73 21.857000983240464 28.346042370700914 27.266082846034223 22.530873800024395 74-75 21.778263490101484 28.722322813288358 27.080253563561634 22.419160133048525 76-77 21.91340976964169 28.35670167494034 27.32897201107656 22.400916544341413 78-79 22.05087520071627 28.389249929195277 27.222793022296266 22.337081847792188 80-81 22.15760307149614 28.404227973993322 27.043472613900203 22.394696340610334 82-83 22.115677354981077 28.50120878423541 27.127236794053832 22.255877066729678 84-85 21.975436137463987 28.50123693489065 27.180489765962733 22.342837161682628 86-87 21.899537822523552 28.393209149321326 27.299189109037293 22.40806391911783 88-89 22.130892729671423 28.227200144595198 27.293405535457797 22.34850159027558 90-91 22.146666162959605 28.299892369652834 27.22753954162164 22.325901925765926 92-93 22.11156686861344 28.202069575832763 27.240937580865953 22.445425974687844 94-95 21.98700201061182 28.041331245766987 27.44938223624578 22.52228450737541 96-97 22.09841523656388 27.88437464042695 27.372762375362413 22.64444774764676 98-99 21.783156334897505 28.054902445048157 27.318918251420104 22.84302296863423 100-101 21.781433946459387 28.187919628825124 27.426154710856785 22.604491713858703 102-103 21.72469258535482 28.034699494436932 27.432562370639907 22.80804554956834 104-105 21.677946935855452 28.281392729443194 27.216321623864864 22.824338710836493 106-107 21.706847058951386 28.245952115491107 27.160283728583646 22.88691709697386 108-109 21.764013208074083 28.37323550160039 26.902673047326296 22.96007824299923 110-111 21.737826061835264 28.45988144956866 27.061508838935666 22.74078364966041 112-113 21.779256096789997 28.638688143067125 27.198578337627033 22.383477422515842 114-115 21.564740929334306 28.865633589984697 26.95585206349818 22.613773417182824 116-117 21.628733006187833 28.760229884603145 26.987873350026582 22.62316375918244 118-119 21.65183544292818 28.640497728417742 26.913634276371006 22.79403255228307 120-121 21.609535239870414 28.833396576934916 26.939673020279564 22.617395162915106 122-123 21.697547054064387 28.874866268955728 26.942248933856106 22.48533774312378 124-125 21.60595542546751 28.911839806743945 26.74335707087872 22.738847696909826 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2644.0 1 2045.0 2 1347.5 3 1115.5 4 814.5 5 620.0 6 544.0 7 504.5 8 546.0 9 540.5 10 475.0 11 435.5 12 444.5 13 489.5 14 547.5 15 584.0 16 664.0 17 819.0 18 981.0 19 1207.0 20 1576.0 21 2198.0 22 2902.5 23 3912.0 24 5410.5 25 7733.5 26 11564.0 27 15524.5 28 18481.0 29 22033.0 30 26658.5 31 32675.0 32 39871.5 33 45638.0 34 50786.0 35 55875.5 36 61629.5 37 67156.0 38 67737.0 39 67229.5 40 67973.5 41 68272.0 42 67757.5 43 67942.5 44 69853.5 45 71618.5 46 71438.5 47 70975.5 48 71110.5 49 70739.5 50 69514.0 51 66357.0 52 63493.5 53 63042.0 54 62332.0 55 57333.0 56 51244.0 57 46552.5 58 43138.0 59 38619.0 60 31405.0 61 25457.0 62 21328.5 63 16468.5 64 11999.5 65 9053.5 66 6800.0 67 5471.0 68 4370.5 69 3104.5 70 2124.0 71 1433.5 72 1103.5 73 903.0 74 628.0 75 453.0 76 330.5 77 201.5 78 131.0 79 72.5 80 46.5 81 32.0 82 22.5 83 17.0 84 13.0 85 12.0 86 13.0 87 10.5 88 6.0 89 5.5 90 8.0 91 8.5 92 6.0 93 4.0 94 4.0 95 5.5 96 3.0 97 0.5 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06365413238281763 2 0.05145663765934521 3 0.04691344124412471 4 0.04656776325601011 5 0.04562949443112761 6 0.0051357872519883895 7 6.913559762292062E-4 8 0.0 9 0.0011357991038051245 10-11 0.00367900144493399 12-13 0.0027901151897821537 14-15 0.008246889145019818 16-17 0.0031851757476274146 18-19 0.00533331753091102 20-21 0.01024688321911145 22-23 0.017086369126807523 24-25 0.003802457869260634 26-27 0.01683945627815424 28-29 0.008074050150962516 30-31 0.0070617074714840345 32-33 0.00286418904437814 34-35 0.018962906776572513 36-37 0.01654316085977029 38-39 0.027259178491322988 40-41 0.006666646913638774 42-43 0.0018024637951690018 44-45 1.9753027892263037E-4 46-47 0.002617276195724852 48-49 0.0035308537357420173 50-51 0.002222215637879591 52-53 0.0018765376497649885 54-55 0.006370351495254829 56-57 0.003382706026550045 58-59 0.00837034556934646 60-61 0.017061677841942197 62-63 0.012617246566183014 64-65 0.0030864106081660994 66-67 0.00409875328764458 68-69 0.011111078189397957 70-71 0.002987645468704784 72-73 0.0034073973114153735 74-75 0.0015061683767850565 76-77 4.197518427105895E-4 78-79 0.00348147116601136 80-81 0.0031851757476274146 82-83 0.0018518463648996594 84-85 0.011999964444549795 86-87 0.006641955628773445 88-89 0.002098759213552948 90-91 9.62960109747823E-4 92-93 0.002469128486532879 94-95 2.4691284865328796E-5 96-97 0.0017283899405730156 98-99 0.02498758028371274 100-101 0.014814770919197275 102-103 0.006666646913638774 104-105 0.015086375052715895 106-107 0.03343199970765519 108-109 0.0016049335162463716 110-111 0.002222215637879591 112-113 0.016493778290039635 114-115 0.03281471758602197 116-117 0.051061577101499944 118-119 0.36343102193277455 120-121 0.1186910063476355 122-123 0.020740679286876187 124-125 0.3231595363174233 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2025006.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.35427070566309 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95888956842043 48.99359770022478 2 8.85523114096299 10.334811103219446 3 2.862509861775909 5.011190261151048 4 1.297843055283377 3.0293873992588423 5 0.6950296019674509 2.0278972770828947 6 0.4422831789688408 1.5485467412465383 7 0.29256371231531647 1.1950639446971203 8 0.203755613822302 0.9512008197428128 9 0.16796129900786397 0.8821133199341744 >10 1.013767619882382 11.646673795405203 >50 0.13335869879018014 5.460468780296004 >100 0.07469657603529141 7.837705763420007 >500 0.001899065490929809 0.6560460039333662 >1k 2.1100727676997877E-4 0.425297090387734 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3454 0.1705673958496913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9753027892263034E-4 2 9.876513946131517E-5 0.0 0.0 0.0 1.9753027892263034E-4 3 9.876513946131517E-5 0.0 0.0 0.0 1.9753027892263034E-4 4 9.876513946131517E-5 0.0 0.0 0.0 1.9753027892263034E-4 5 1.4814770919197276E-4 0.0 0.0 0.0 1.9753027892263034E-4 6 1.4814770919197276E-4 0.0 0.0 0.0 3.950605578452607E-4 7 2.4691284865328795E-4 0.0 0.0 0.0 3.950605578452607E-4 8 3.456779881146031E-4 0.0 0.0 0.0 3.950605578452607E-4 9 3.456779881146031E-4 0.0 0.0 0.0 3.950605578452607E-4 10-11 3.950605578452607E-4 0.0 0.0 0.0 3.950605578452607E-4 12-13 4.1975184271058946E-4 0.0 0.0 0.0 7.654298308251926E-4 14-15 6.913559762292062E-4 0.0 0.0 0.0 8.888862551518366E-4 16-17 7.407385459598638E-4 0.0 0.0 2.4691284865328793E-5 0.0010123426794784807 18-19 0.0010123426794784805 0.0 0.0 4.9382569730657585E-5 0.0011357991038051245 20-21 0.0012345642432664396 0.0 0.0 9.876513946131517E-5 0.0011604903886704532 22-23 0.001333329382727755 0.0 0.0 3.703692729799319E-4 0.0012839468129970974 24-25 0.0017283899405730156 0.0 0.0 9.135775400171654E-4 0.0012839468129970974 26-27 0.0017530812254383443 0.0 0.0 0.0017283899405730156 0.001308638097862426 28-29 0.002148141783283605 0.0 0.0 0.0032345583173580723 0.001333329382727755 30-31 0.0023209807773409068 0.0 0.0 0.010938239195340656 0.0013580206675930838 32-33 0.0023456720622062354 0.0 0.0 0.028641890443781404 0.001456785807054399 34-35 0.0024444372016675505 0.0 0.0 0.054518356982645975 0.0016296248011117005 36-37 0.002518511056263537 0.0 0.0 0.09622193712018631 0.0016296248011117005 38-39 0.002765423904916825 0.0 0.0 0.15565385979103272 0.0017283899405730156 40-41 0.002765423904916825 0.0 0.0 0.21888824033113974 0.0017283899405730156 42-43 0.003111101893031428 0.0 0.0 0.2791596666874073 0.0017283899405730156 44-45 0.003358014741684716 0.0 0.0 0.3570359791526544 0.0017777725103036732 46-47 0.004197518427105895 0.0 0.0 0.4523937213025542 0.0018271550800343307 48-49 0.004493813845489841 0.0 0.0 0.551060095624408 0.0019506115043609747 50-51 0.004493813845489841 0.0 0.0 0.6603437224383533 0.0019753027892263036 52-53 0.004518505130355169 0.0 0.0 0.7686643891425506 0.002172833068148934 54-55 0.004592578984951155 0.0 0.0 0.8861455225317851 0.002172833068148934 56-57 0.004938256973065758 0.0 0.0 1.0249105434749328 0.002172833068148934 58-59 0.004938256973065758 0.0 0.0 1.1785644091918739 0.002172833068148934 60-61 0.005012330827661745 0.0 0.0 1.351354020679445 0.002172833068148934 62-63 0.005037022112527074 0.0 0.0 1.5249337532827063 0.00266665876545551 64-65 0.005086404682257731 0.0 0.0 1.7083899998320993 0.00266665876545551 66-67 0.0052839349611803615 0.0 0.0 1.9144881546029988 0.0027407326200514963 68-69 0.0052839349611803615 0.0 0.0 2.156610893992413 0.0028148064746474823 70-71 0.005333317530911019 0.0 0.0 2.4371779639171436 0.002839497759512811 72-73 0.005382700100641677 0.0 0.0 2.71660923473807 0.0029382628989741266 74-75 0.005382700100641677 0.0 0.0 2.9928059472416377 0.0030370280384354417 76-77 0.005382700100641677 0.0 0.0 3.3046815663756055 0.0031604844627620855 78-79 0.005382700100641677 0.0 0.0 3.649100298962077 0.0031604844627620855 80-81 0.0059259083676789105 0.0 0.0 4.031395462531963 0.003185175747627414 82-83 0.0059259083676789105 0.0 0.0 4.442999181236994 0.0034073973114153735 84-85 0.006172821216332199 0.0 0.0 4.847812796604059 0.0034320885962807026 86-87 0.0064197340649854865 0.0 0.0 5.272206600869331 0.00348147116601136 88-89 0.006469116634716144 0.0 0.0 5.731168203946062 0.0035061624508766886 90-91 0.006938251047157391 0.0 0.0 6.240327189154007 0.0035061624508766886 92-93 0.00716047261094535 0.0 0.0 6.785041624568025 0.003728384014664648 94-95 0.00716047261094535 0.0 0.0 7.343805401070417 0.0037777665843953057 96-97 0.00716047261094535 0.0 0.0 7.887630950229283 0.004345666136297868 98-99 0.007506150599059954 0.0 0.0 8.467407010152069 0.004740726694143128 100-101 0.007851828587174557 0.0 0.0 9.101380440354252 0.004740726694143128 102-103 0.008296271714750475 0.0 0.0 9.785872239390896 0.004765417979008457 104-105 0.009555527242882243 0.0 0.0 10.477623276177948 0.004790109263873786 106-107 0.009876513946131517 0.0 0.0 11.150979305740329 0.004790109263873786 108-109 0.010074044225054148 4.9382569730657585E-5 0.0 11.839051341082445 0.004790109263873786 110-111 0.010148118079650135 4.9382569730657585E-5 0.0 12.586283694961892 0.004839491833604444 112-113 0.010271574503976779 4.9382569730657585E-5 0.0 13.366923357264128 0.004839491833604444 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2150 0.0 57.00479 1 GTATCAA 4005 0.0 46.6455 1 ATCAACG 4415 0.0 39.615677 3 CAACGCA 4485 0.0 38.999294 5 AACGCAG 4650 0.0 37.856373 6 TACATGG 9595 0.0 37.20118 2 TCAACGC 4690 0.0 37.165028 4 GTACATG 9830 0.0 36.98029 1 ACATGGG 9625 0.0 35.292774 3 TATCAAC 5180 0.0 34.339333 2 CATGGGA 5310 0.0 31.593248 4 CGCAGAG 5685 0.0 30.545126 8 ACGCAGA 5765 0.0 30.224411 7 CATGGGG 4465 0.0 28.11258 4 ATGGGAT 2425 0.0 25.024208 5 GCAGAGT 6760 0.0 24.194 9 ATGGGAG 2660 0.0 23.260744 5 TAGCCCG 180 2.17986E-5 23.136486 5 AGAGTAC 6030 0.0 21.895626 10-11 CAGAGTA 6470 0.0 21.325804 10-11 >>END_MODULE