FastQCFastQC Report
Thu 26 May 2016
SRR1364176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364176_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025006
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83180.41076421501960986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74600.36839397019070563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44830.22138206010253797No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.075.391
GTATCAA83050.052.546811
ATCAACG104000.041.3707923
CAACGCA105900.040.7308735
TGGTATC17050.040.4906432
TCAACGC108250.039.911464
TATCAAC108250.039.8044622
AACGCAG109600.039.4614756
ACGCAGA128500.033.6102877
CGCAGAG129000.033.295558
GTACATG92050.031.3689481
GTGGTAT22650.031.279571
TACATGG93400.030.9040892
ACATGGG90700.029.984573
GCAGAGT144150.029.5073459
GAGTACT96150.027.65853512-13
CATGGGG42400.026.9478594
CAGAGTA139250.026.70075610-11
CATGGGA48800.025.852644
GTACTTT103050.025.66224714-15
Produced by FastQC (version 0.11.5)