FastQCFastQC Report
Thu 26 May 2016
SRR1364175_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364175_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411852
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.053.539081
GTATCAA32200.042.6882741
ATCAACG34250.037.865993
TCAACGC35050.036.663014
CAACGCA35400.035.9658975
AACGCAG36450.034.915366
GTACATG81400.032.968911
TATCAAC41100.032.7156872
TACATGG82550.031.7841722
ACATGGG80750.031.0901813
ACGCAGA43950.028.4152667
CGCAGAG45050.027.5894348
CATGGGG52600.025.6745284
GTAAGGT9850.022.3474454
TAAGGTG11200.021.7794155
GAGTACA61600.021.3483371
GTATAGG7350.021.049341
GTGTAGC16400.020.3187351
GGTAAGG10550.020.3003443
GCAGAGT61250.019.9051789
Produced by FastQC (version 0.11.5)