FastQCFastQC Report
Thu 26 May 2016
SRR1364175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364175_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411852
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52730.2186286720744059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.2141507853715734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.12384673686445105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38000.070.813531
GTATCAA66700.050.8757061
ATCAACG84200.039.6456953
TCAACGC86600.038.546174
CAACGCA87050.038.340555
TATCAAC90200.037.4726222
AACGCAG89850.037.2104076
GTGGTAT19150.035.1294331
ACGCAGA104600.031.9063727
TGGTATC19800.031.5570532
CGCAGAG105500.031.5214068
GTACATG81400.029.1816561
TACATGG82300.028.2715232
ACATGGG80100.027.6347583
GCAGAGT128550.025.6379859
GAGTACT84650.025.08975612-13
AGAGTAC114950.024.19462210-11
CAGAGTA122350.024.1899710-11
GTACTTT91450.023.09403614-15
CATGGGG54900.022.868964
Produced by FastQC (version 0.11.5)