FastQCFastQC Report
Thu 26 May 2016
SRR1364174_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364174_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217070
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19450.048.018881
GTATCAA31450.045.0182151
ATCAACG33150.039.6553273
TCAACGC33450.039.1209644
CAACGCA34100.038.2034075
AACGCAG35550.036.970676
TATCAAC41650.033.134132
GTACATG72250.032.7700581
TACATGG74850.030.2785992
ACATGGG74400.030.0612953
ACGCAGA44000.029.4631427
CGCAGAG44600.028.6667758
CATGGGG50750.025.4335334
GCAGAGT57950.021.1416079
TATACGG1703.9416226E-420.9949465
GTGGTAT15700.019.703151
AGAGTAC49050.019.27890210-11
CAGAGTA53800.018.79277210-11
GAGTACA55550.018.6336171
CGTTCGG3050.018.5425534-35
Produced by FastQC (version 0.11.5)