FastQCFastQC Report
Thu 26 May 2016
SRR1364174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364174_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217070
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56470.254705534782393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53550.24153499889493793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28930.13048753535071062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34750.068.705331
GTATCAA60300.049.6651231
ATCAACG73450.040.6675643
TCAACGC74950.039.853674
CAACGCA77450.038.5602765
AACGCAG79050.038.1543776
TATCAAC79200.037.8695872
GTGGTAT16200.034.547261
ACGCAGA93000.032.302567
CGCAGAG94050.031.9419218
GTACATG69800.031.0489391
TGGTATC17750.029.838492
ACATGGG71900.029.1306383
TACATGG73750.029.0486352
GCAGAGT109200.027.2380399
GAGTACT73950.025.6226612-13
CATGGGG48850.024.4831754
CAGAGTA105550.024.43201810-11
GTACTTT79600.023.58001914-15
AGAGTAC101250.023.0901110-11
Produced by FastQC (version 0.11.5)