FastQCFastQC Report
Thu 26 May 2016
SRR1364173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128678
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36660.17221956538283387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.059.1330451
GTATCAA40700.046.48981
ATCAACG45750.038.490553
GTACATG106500.038.047181
TCAACGC46250.037.6867754
CAACGCA47700.036.41735
TACATGG107200.036.2403452
AACGCAG49500.034.9621736
ACATGGG108700.034.589243
TATCAAC54800.033.6554262
ACCGTAC1004.4547138E-429.7330788
CATGGGA65900.028.976954
CATGGGG47400.028.8657344
ACGCAGA61350.027.8193747
CGCAGAG61950.027.45338
CATACCG1150.001006090125.8651775
ATGGGAG32300.023.7591275
GCAGAGT68750.023.7027459
GTACCGT2052.2739932E-623.2084836
GAGTACA78400.022.5405921
Produced by FastQC (version 0.11.5)